Manuel Del Valle Ortiz

RISE Program : Summer 2010

Proposal

What is nanotechnology?

Tissue regeneration is actual full scale project in nanotechnology that has not yet reach its total development.

Biotechnology is also a main component in this research.

In this research, tissue regeneration will be exposed to many test on “nanotech” working as a extracellular matrix and a scaffold sending different messages to the cell. The final result of this tests should be an acceleration on tissue regeneration.

By making a correct design of a scaffold for tissue regeneration we can recapitulate extracellular matrix (ECM) function in a temporally coordinated and spatially organized structure which will allowed us to accelerate the process for tissue regeneration.

To obtain a new class of hybrid materials, capable of directly interacting with the cell.

To establish the necessary procedure to grow skin tissue in the designed material.

Nanofabricated and microfabricated tissue engineering scaffolds have the potential to direct cell fate as well as to regulate processes.

Synthetic polymeric nanofibers, obtained by electrospinning, can be used to make a highly porous non-woven structure comprising tiny fibers where we can control cells growth.

Controlled growth factor delivery systems can be used, in combination with techniques to produce porous scaffolds, to provide guidance in morphogenesis.

In the guiding of a cell though a tailor made scaffold, the ability to elicit a specific response to signal gradient is a key design objective.

The appropriate attachment of the requisite signal species to the scaffold help cell guidance through the scaffold and all aspects of cell response—adhesion and migration, proliferation and phenotype choice—proceed perfectly.

For scaffold materials, there must be the correct balance between architectural features notably porosity and physical properties.

For this experiment I will be using tissue culture.

Skin tissue cells will be purchased to grow them in the scaffold material.

We will apply different growth factors to control cell division.

This experiment is necessary to be repeated many times before getting to any conclusion.

Many different results may occur during the cell regeneration process.

Therefore I must keep track of any changes to the tissue. This changes are to be compared to other tissue culture cells in normal growing conditions.

If the experiment show some failure rate then it must be reorganized and changes in materials and procedure must be done.

If the experiment is successful then other steps will be taken to use the tissue in experimental animals and latter on humans.

This is an important experiment to acknowledge the regenerative functions of a scaffold.