ncrna jeremy logue. the expanding repertoire of rna adapted from soares and valcarcel (2006) embo
TRANSCRIPT
ncRNAncRNA
Jeremy LogueJeremy Logue
The Expanding Repertoire of RNA
Adapted from Soares and Valcarcel (2006) EMBO
Adapted from Huttenhofer, A et al. (2005) Trends in Genetics
The Expanding World of Non-coding RNAs
HAR1-associated transcripts in genomic context
Of the 49 HARS identified (Human Accelerated Regions),96% are in non-coding regions.
Predicted RNA secondary structure for HAR1F
Expression of HAR1F and HAR1R in the developing neocortex
Cajal-Retzius cells establish early neuronal circuitry in the developing brain
Expression of HAR1F in other parts of the developing brain
What does this all mean?
Fill in the blank…
X - InactivationX - Inactivation
Dosage Compensation
Adapted from Straub and Becker (2007) Nature Reviews Genetics
Imprinted Versus Random X-inactivation
Adapted from Avnir and Heard (2001) Nature Reviews Genetics
The X-inactivation Center
Adapted from Avnir and Heard (2001) Nature Reviews Genetics
Differentiation
Xist Transcription in Embryonic Stem Cells
Adapted from Avnir and Heard (2001) Nature Reviews Genetics
48 hrs.
Adapted from Avnir and Heard (2001) Nature Reviews Genetics
Potential Roles for Xist Antisense Transcription
What does a silenced X look like?
Adapted from Chow, JC et al. (2005) Annu. Rev. Genomic Hum. Genet.
Co-localization and 3D - Microscopy
How confident are we that these co-localize?
Figure 1G from Chaumeil, J. et al. (2007) Gen Dev
Problems Associated with Co-localization
Resolution = λ/(2NA)
z-axis problem
Calculating a cross-correlation can also aid in determining co-localization
3D - Microscopy