Discoversuperior nucleic acid purification

Applications Products to use Cat. #

PCR clean-up

Removal of nucleotides

GeneJET PCR Purification Kit K0701/2

FastAP Thermosensitive Alkaline Phosphatase

EF0651/2/4

Removal of primers

GeneJET PCR Purification Kit K0701/2

Exonuclease I EN0581/2

Silica Bead DNA Gel Extraction Kit K0513

Extraction of DNA from agarose gel

Standard agarose gel

GeneJET Gel Extraction Kit K0691/2

Silica Bead DNA Gel Extraction Kit K0513

TopVision Agarose R0491/2

Low melting point agarose gel

Silica Bead DNA Gel Extraction Kit K0513

GeneJET Gel Extraction Kit K0691/2

Agarase EO0461

TopVisionTM Low Melting Point Agarose

R0801

Removal of RNA from DNA preparations

RNase A, DNase and protease-free EN0531

RNase T1 EN0541/2

RNase A/T1 Mix EN0551

RNase I EN0601/2

Removal of DNA from RNA preparations

DNase I, RNase-free EN0521/3/5

Removal of proteins from DNA and RNA preparations

Proteinase K (recombinant), PCR grade

EO0491/2

DNA and RNA precipitation

Glycogen, RNA grade R0551

Glycogen, molecular biology grade R0561

3 M Sodium Acetate Solution, pH 5.2

R1181

* available in EU countries only

for all your molecular biology needs

thermoscientific.com/onebio

Applications Products to use Cat. #

Isolation of plasmid DNA

GeneJETTM Plasmid Miniprep Kit K0502/3GeneJET Plasmid Midiprep Kit K0481/2GeneJET Plasmid Maxiprep Kit K0491/2MagJETTM Plasmid DNA Kit K2791/2

Isolation of RNA

GeneJET RNA Purification Kit K0731/2GeneJET Whole Blood RNA Purification Mini Kit

K0761

GeneJET Plant RNA Purification Mini Kit

K0801/2

MagJET RNA Kit K2731/2MagJET Whole Blood RNA Kit K2751/2MagJET Plant RNA Kit K2771/2

Isolation of genomic DNA

GeneJET Genomic DNA Purification Kit

K0721/2

Genomic DNA Purification Kit K0512GeneJET Whole Blood Genomic DNA Purification Mini Kit

K0781/2

GeneJET Plant Genomic DNA Purification Mini Kit

K0791/2

Proteinase K (recombinant), PCR grade

EO0491/2

MagJET Genomic DNA Kit K2721/2MagJET Whole Blood Genomic DNA Kit

K2741/2

MagJET Plant Genomic DNA Kit K2761/2

Isolation of viral DNA and RNA

GeneJET Viral DNA and RNA Purification Kit

K0821

Viral DNA/RNA Purification Kit, (IVD)* DK0011MagJET Viral DNA and RNA Kit K2781/2

Thermo ScientificTM manual and automated nucleic acid purification solutions provide high quality DNA and RNA that is ready to use in routine or very demanding downstream applications.

• Find your solution and request your free sample at thermoscientific.com/yieldpurityvaluefis

Thermo Scientific GeneJET Nucleic Acid Purification Kits• Trusted silica membrane technology in the form of a convenient spin column• Superior purity and yields compared to other kits on the market• Extensively tested on a wide variety of sample types and include specialized protocols

thermoscientific.com/onebio © 2013 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

Plasmid DNA purification

DNA,

µg

250

200

150

100

50

0Thermo

Scientificvendor A vendor B vendor C

1st elution

2nd elution

vendor D

DNA,

ng/

µg

900

700

500

300

1000

Thermo Scientific

vendor A vendor B vendor C

1st elution

2nd elution

vendor D

200

400

600

800

Yields of high-copy plasmid DNA obtained using Thermo ScientificTM GeneJETTM Plasmid Midiprep Kit and other kits for midscale plasmid DNA purification at maximal recommended bacterial cell culture volumes.

Concentrations of eluted DNA obtained after the purification of high-copy plasmid DNA using different kits for midscale plasmid DNA purification at maximal recommended bacterial cell culture volumes.

Genomic DNA purification

M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M

Genomic DNA from different plant material samples purified using the Thermo ScientificTM GeneJETTM Plant Genomic DNA Purification Mini Kit.1- Arabidopsis, 2- Tobacco leaves, 3- Tomato leaves, 4- Spinach leaves, 5- Wheat leaves, 6- Grape leaves, 7- Onion leaves, 8- Rice leaves, 9- Corn seeds, 10- Sunflower stalk, 11- Corn roots, 12- Beef roots, 13- Cucumber fruit, 14 - Sugar cane.

Total RNA purification

Total RNA purified from 50 mg of tomato leaves using the Thermo ScientificTM GeneJETTM Plant RNA Purification Kit and kits from other vendors. M- Thermo Scientific™ RiboRuler™ High Range RNA Ladder. 1- Thermo ScientificTM product, 2-5 other vendors.

Thermo Scientific MagJET Nucleic Acid Purification Kits • Compatible with a wide variety of sample

types, including cells and tissues, whole blood, plants, serum, plasma, and bacterial culture

• Protocols for automated and manual workflows provided

• Nucleic acid isolation via magnetic beads

M 1 2 3 4 5 6

Total RNA purified from various sources using the Thermo ScientificTM GeneJETTM RNA Purification Kit. M- RiboRuler High Range RNA Ladder (#SM1821) 1- mouse liver, 2- mouse spleen, 3- HeLa cells, 4- Cos-7 cells, 5- Saccharomyces cerevisiae, 6- Escherichia coli DH5α.

M 1 2 3 4 5

“Surprisingly, the cheapest kit we tested turned out

to have the best performance. It has the highest

yield on each of the tested plasmids. The purity of

the GeneJET elutions are also appreciable, closely

following that of the QIAprep kit. Finally, its buffers,

like the innuPrep kit’s, are named instead of coded,

which makes using the kit very easy and intuitive ...

we conclude that the GeneJET kit, of all those we

compared, delivers the biggest bang for its buck.”

-iGEM team member

Manual and automated workflows were used to purify RNA from N.tobacum leaf samples (each 50 mg) with the Thermo Scientific™ MagJET™ Plant RNA Kit. The Thermo Scientific™ KingFisher™ protocol was used for the automated workflow. Isolated RNA was analyzed by agarose gel electrophoresis (1%), M – Thermo Scientific™ RiboRuler ™ High Range RNA Ladder (Cat #SM1823).

Austria: +43 (0) 800 20 88 40 Belgique/België: +32 (0) 056 260 260 Denmark: +45 070 27 99 20 España: +34 902 239 303 Finland: +358 (0) 9 8027 6280 France: +33 (0) 3 88 67 53 20 Germany: +49 (0) 180 5 258221 Ireland: +353 (0) 1 885 5854 Italia: +39 02 953 28 258 Netherlands: +31 (0) 20 487 70 00 Norway: +47 (0) 22 95 59 59 Portugal: +351 21 425 33 50/4 Suisse/Switzerland: +41 (0) 56 618 41 11 Sweden: +46 (0) 31 352 32 00 UK: +44 (0)1509 23 11 66M

B134

0CCG

• Find your solution and request your free sample at thermoscientific.com/yieldpurityvaluefis