TABLE 1. compares obese and nonobese patients.

COMPARISON BY BMI

BMI<30 (n¼92) BMI>30 (n¼25) p

BMI 23.6 (3) 35.1 (5)Peak E2 3059.3 (1003) 2506.8 (861.4) 0.009Total FSH 1831.2 (931) 2478.3 (1224.2) 0.003Days stim 8.6 (1.7) 9.3 (2) 0.087Fert embryos 10.6 (4.3) 9.52 (3.5) 0.205Mature oocytes 11.7 (3.8) 11.3 (4) 0.617Blast formation rate 57.9% (0.2) 53.3% (0.3) 0.403

Results are mean (SD).

or absence of multinucleation (r¼0.047) or the percentage of multinucleatedembryos obtained from a single patient (r¼0.088).

CONCLUSION: Despite previously documented independent associa-tions of obesity and embryo multinucleation with poor IVF cycle character-istics, obesity did not correlate with the presence of multinucleated humanembryos in our dataset.

P-664 Wednesday, October 16, 2013

NEONATAL OUTCOMES FOR OBESE WOMEN UNDERGOING INVITRO FERTILIZATION (IVF). M.-Y. M. Leung,a S.-H. Chang,a

L. M. Pollack,c J. S. Rhee,b E. S. Jungheim.b aPublic Health Sciences,Department of Surgery, Washington University, St. Louis, MO; bReproduc-tive Endocrinology and Infertility, Department of Obstetrics and Gynecol-ogy, Washington University, St. Louis, MO; cGeorge Warren BrownSchool of Social Work, Washington University, St. Louis, MO.

OBJECTIVE: Previous work investigating IVF outcomes in obese womenfocuses largely on clinical pregnancy rates, live birth rates andmaternal com-plications, but little data exists regarding neonatal outcomes for obesewomen using IVF. Therefore, we investigated gestational age and birthweight in children born to obese women after IVF.

DESIGN: Retrospective cohort.MATERIALS AND METHODS: Inclusion criteria were women with a

singleton delivery after their first IVF cycle in our center between 2001and 2008.Women using donor oocytes were excluded. Associations betweenmaternal bodymass index (BMI), gestational age and birth weight at deliverywere investigated after classifying women into one of three BMI groups:normal (less than 25 kg/m2), overweight (25-29 kg/m2), and obese (greaterthan or equal to 30 kg/m2). T- tests and chi-square analysis were used todetermine differences in neonatal outcomes between the normal-weightgroup and the other groups.

RESULTS: 206 women met study criteria. Obese women had a signif-icantly higher fraction of preterm births than normal-weight women (37vs. 21%, p¼0.01). Overweight women had a larger fraction of large forgestational age (LGA) infants (31 vs. 20%, p¼0.05) when compared tonormal-weight women. Overweight women had heavier infants comparedto normal-weight women, although this difference was not statisticallysignificant (7.02 vs. 6.74 lbs, p¼0.1). Overall infants born to obesewomen weighed significantly less when compared to infants born tonormal weight women (6.29 vs. 6.74 lbs, p¼0.05), which may be attrib-uted to the significantly higher fraction of preterm births among obesewomen.

CONCLUSION: Our findings provide evidence that higher maternalBMI in IVF patients translates into adverse neonatal outcomes includinghigher incidence of preterm birth, and for infants born at term an increasedrisk of LGA. This is concerning as preterm birth and LGA at term areknown to adversely impact chronic health conditions and development inchildren.

Supported by: K12HD063086.

P-665 Wednesday, October 16, 2013

OVARIAN UTILIZATION OF STORED LIPID DURING INDUCEDFOLLICULOGENESIS IN THE PLIN2-NULLMOUSE. S. M. Stephens,a E. S. Bales,b D. J. Orlicky,c A. J. Polotsky,a

J. Monks,b J. L. McManaman.b aObstetrics and Gynecology, University of

S340 ASRM Abstracts

Colorado, Denver, Aurora, CO; bBasic Reproductive Sciences, Universityof Colorado School of Medicine, Aurora, CO; cPathology, University of Col-orado School of Medicine, Aurora, CO.

OBJECTIVE: Intracellular lipid droplets (LD) have been identified in theovary and may serve as reservoirs of cholesterol for sex hormone productionduring folliculogenesis. Perilipin 2 (Plin2) is a LD-associated protein thatmoderates the storage and metabolism of lipid within LDs. Our objectivewas to evaluate the utilization of LDs in the ovary during folliculogenesisin the Plin2-null mouse.DESIGN: Comparative.MATERIALS AND METHODS: C57Bl6 (WT) and Plin2-null mice

received 5 IU pregnant mare’s serum (PMS) IP at 23 days of age followedby 5 IU human chorionic gonadotropin (hCG) IP 46-48 h later. Mice wereeuthanized at 48 h after PMS or 12 h after hCG. Ovaries were frozen,sectioned and slide mounted. BODIPY 493/503 and Hoescht were used tostain LDs and nuclei respectively. Slides were imaged by laser confocal mi-croscope at 630x. LDs were quantified by BODIPY staining normalized tonuclear staining within the granulosa and theca of antral follicles using Slide-book 5.5. The median and interquartile range (IQR) was calculated andcompared by Mann-Whitney test (STATA 11.0). p<0.05 was considered sig-nificant. 8-12 follicles/strain were imaged per timepoint.RESULTS: Intracellular LD stores did not differ between WT and Plin2-

null mice. WT mice demonstrated a significant decrease in LD in theca cellsduring folliculogenesis. Plin2-null mice did not show a decrease in LD withstimulation in either granulosa or theca.

Intracellular Lipid Stores During Ovarian Stimulation as Measured by Area of

BODIPY Stain (mm2)

48 h p PMS 12 h p hCG p value

Vo

l. 100, No. 3, Su pplement, Septem

Granulosa

WT 0.03 (0.01-0.06) 0.001 (0.000-0.04) 0.08 Plin2-null 0.01 (0.001-0.04) 0.01 (0.003-0.03) 0.66

Theca

WT 0.06 (0.02-0.14) 0.001 (0.000-0.05) 0.02 Plin2-null 0.03 (0.01-0.09) 0.02 (0.01-0.05) 0.53

Data presented as median (25%-75% IQR).

CONCLUSION: Theca cells utilize intracellular LD during folliculogen-esis. Plin2-null mice have impaired ability to utilize LD in the ovary. The ef-fects of altered ovarian lipid utilization during folliculogenesis on sexhormone production and fertility warrant further investigation.Supported by: UCD Academic Enrichment Fund.

P-666 Wednesday, October 16, 2013

OBESITY AND POLYCYSTIC OVARIAN SYNDROME: IS THEREA LINK BETWEEN DYSFUNCTIONAL SLEEP AND IVFOUTCOMES? R. Flyckt,a S. Ibrahim,b J. Tantibhedhyangkul.a aRepro-ductive Endocrinology and Infertility, Cleveland Clinic, Cleveland, OH;bNeurology, Cleveland Clinic, Cleveland, OH.

OBJECTIVE: The area of sleep disorders and infertility is essentially un-explored. However, previous research has established a link between polycy-stic ovarian syndrome (PCOS), a common cause of anovulation andinfertility, and obstructive sleep apnea (OSA). The present study aims toassess the relationship between sleep patterns and clinical outcomes within vitro fertilization.DESIGN: Retrospective cohort study.MATERIALS AND METHODS: Women >21 years old who were seen

for infertility visits at our institution between 2009 and 2011 were identified.Patients were contacted by email to complete an online questionnaire assess-ing demographics, sleep patterns, and fertility treatments. Spearman andPearson correlation coefficients for scores on standardized sleep measuresand IVF variables were generated for ordinal and ratio data, respectively.RESULTS: 79 patients completing 131 fresh and 90 frozen IVF cycles

participated in the online survey. Most patients were Caucasian (94%). Me-dian age and BMI were 36 [32,40] and 23.49 [22, 27, respectively]. 12 re-spondents (15%) indicated their diagnosis as anovulation or PCOS.Relevant findings are noted in Table 1. There is a positive association be-tween daytime sleepiness and more retrieved oocytes and clinical pregnan-cies which persisted when first cycles were independently analyzed.

ber 2013