neuropathology of olfactory ensheathing cell transplantation in als
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DESCRIPTION
Although a large number of amyotrophic lateral sclerosis (ALS) patients have undergone transplantation procedures with olfactory ensheathing cells (OECs) in the Bejing Hospital, to our knowledge, no post-mortem neuropathologic analyses have been performed. We examined the post-mortem brain of two Italian patients affected by ALS who underwent cellular transplantation in Beijing with their consent. Our aim was to assess the events following the graft procedure to possibly support the rationale of the treatment strategy.TRANSCRIPT
- 1. NEUROPATHOLOGY OF FETAL OLFACTORY ENSHEATHING CELLS
TRANSPLANTATION IN ALS PATIENTS
Silvia Grifoni
Dipartimento di Neuroscienze
Laboratorio di Neuropatologia
Universit di Torino
2. NEUROPATHOLOGY OF FETAL OLFACTORY ENSHEATHING CELLS
TRANSPLANTATION IN ALS PATIENTS
Amyotrophic lateral sclerosis (ALS)
Progressive loss of motor neurons
Aetiologyunknown
Leadtodeathwithinfewyears
Incurabledisease
3. NEUROPATHOLOGY OF FETAL OLFACTORY ENSHEATHING CELLS
TRANSPLANTATION IN ALS PATIENTS
XiShan HospitalBeijing- China
olfactory ensheathing cell transplantation procedure
2.000.000 OECs
4. NEUROPATHOLOGY OF FETAL OLFACTORY ENSHEATHING CELLS
TRANSPLANTATION IN ALS PATIENTS
5. NEUROPATHOLOGY OF FETAL OLFACTORY ENSHEATHING CELLS
TRANSPLANTATION IN ALS PATIENTS
SecondPatient
First Patient
6. NEUROPATHOLOGY OF FETAL OLFACTORY ENSHEATHING CELLS
TRANSPLANTATION IN ALS PATIENTS
7. NEUROPATHOLOGY OF FETAL OLFACTORY ENSHEATHING CELLS
TRANSPLANTATION IN ALS PATIENTS
Entry hole in frontalcortex
CD68
H&E
vimentin
GFAP
8. NEUROPATHOLOGY OF FETAL OLFACTORY ENSHEATHING CELLS
TRANSPLANTATION IN ALS PATIENTS
The track in the whitematter
H&E
MBP
HLA-DR
Luxol
GFAP
9. NEUROPATHOLOGY OF FETAL OLFACTORY ENSHEATHING CELLS
TRANSPLANTATION IN ALS PATIENTS
The cells inside the track
H&E
CD68
Nestin
-tubulin
10. NEUROPATHOLOGY OF FETAL OLFACTORY ENSHEATHING CELLS
TRANSPLANTATION IN ALS PATIENTS
The inflamation
The glialreaction
GFAP
CLA
GFAP
CD-20
11. NEUROPATHOLOGY OF FETAL OLFACTORY ENSHEATHING CELLS
TRANSPLANTATION IN ALS PATIENTS
What did we find ?
Persistence of quiescent cells within the delivery track
Ongoing inflammation and gliosis in the surroundings
Neither neurites nor myelin within the delivery track
No change in motor cortex and spinal cord
12. NEUROPATHOLOGY OF FETAL OLFACTORY ENSHEATHING CELLS
TRANSPLANTATION IN ALS PATIENTS
What were we not able to do ?
Comparing the phenotype of transplanted cells before and after
transplantation
Tracing the heterologous cells
Assessing the moving of transplanted cells towards pathological
areas, as reported in animal models
13. NEUROPATHOLOGY OF FETAL OLFACTORY ENSHEATHING CELLS
TRANSPLANTATION IN ALS PATIENTS
What the clinical history of the patients tell us ?
No adverse reaction after transplantation
No change of the course of illness
14. NEUROPATHOLOGY OF FETAL OLFACTORY ENSHEATHING CELLS
TRANSPLANTATION IN ALS PATIENTS
CONCLUSIONS
It was an opportunity to study the long-term fate of supposed
neural precursor cell transplanted into the human brain.