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New Fluorochrome Reagents for Immunology and Cell Signaling ResearchFeaturing Alexa Fluor® 488, Alexa Fluor® 647, PE-Cy7, and APC-Cy7 Conjugated Antibodies
Table of Contents
2 www.bdbiosciences.com
* These conjugated products are sold under license to the following patents: US Patent Nos. 4,520,110; 4,859,582; 5,055,556; 5,268,486; 5,486,616; 5,569,587;5,569,766; 5,627,027; and 4,542,104; European Patent No. 76,695; and Canadian Patent No. 1,179,942.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. Cy7 is a trademark of Amersham Biosciences Limited.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details.Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Becton Dickinson and Company is strictly prohibited.BD, BD Logo and all other trademarks are the property of Becton, Dickinson and Company. ©2003 BD
PE-Cy7, APC-Cy7, Alexa Fluor® 488, and Alexa Fluor® 647 Fluorochrome Conjugated Monoclonal Antibodies . . . . . . . . . . . . . . 3
Introductory Offer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Multicolor Analysis Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
New Fluorochrome Reagents for Immunology and Cell Signaling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Antibodies to Non-Human Primate Cell Surface Markers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Antibodies to Human Cell Surface Markers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Isotype Controls for use with Antibodies to Human Cell Surface Markers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Antibodies to Human Cytokines and Chemokine Receptors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Isotype Controls for use with Antibodies to Human Cytokines and Chemokine Receptors . . . . . . . . . . . . . . . . . . . . . . . 7
Antibodies to Mouse Cell Surface Markers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Isotype Controls for use with Antibodies to Mouse Cell Surface Markers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Antibodies to Mouse Cytokines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Isotype Controls for use with Antibodies to Mouse Cytokines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
BD™ PhosFlow Antibody Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Second Step Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
BD Biosciences is continually adding new colors to an expanding panel of reagents including over 100 new PE-Cy7,APC-Cy7, Alexa Fluor® 488, and Alexa Fluor® 647-conjugated monoclonal antibodies for flow cytometric analysis of mouse,human and non-human primate cytokines, chemokine receptors, cell surface antigens, and protein phosphorylation.
New PE-Cy7, APC-Cy7, Alexa Fluor® 488, and Alexa Fluor® 647 Fluorochromes
PE-Cy7, APC-Cy7, Alexa Fluor® 488, and Alexa Fluor® 647Fluorochrome Conjugated Monoclonal Antibodies
3Unless otherwise specified, all products are for Research Use Only.Not for use in diagnostic or therapeutic procedures. Not for resale.
PE-Cy7
PE-Cy7* is a tandem fluorochrome composed of R-phycoerythrin(PE) (excited by the 488-nm line of a laser and serves as an energydonor), coupled to the cyanine dye Cy7™ (acts as an energyacceptor and fluoresces at 785 nm).1 PE-Cy7-labeled antibodiescan be used with FITC and PE-labeled reagents on single-laserflow cytometers with no significant spectral overlap between PE-Cy7 and FITC.1
APC-Cy7
APC-Cy7* is a tandem fluorochrome composed ofAllophycocyanin (APC) (excited by laser lines from 595 to 647nm and serves as an energy donor), coupled to the cyanine dyeCy7™ (acts as an energy acceptor and fluoresces at 785 nm).1,2
Alexa Fluor® 488
Alexa Fluor® 488 is a green fluorescent dye with a fluorescencespectrum almost identical to that of FITC, with excitation/emission maxima of 494/519 nm and a fluorescence lifetime of~4.1 nanoseconds. Alexa Fluor® 488 can be analyzed in the sameinstrument channel as FITC.
Alexa Fluor® 647
Alexa Fluor® 647 can be excited by long-wavelength excitationsources, such as a red HeNe laser (at 633 nm), a krypton-ion laser (at 647 nm), or laser diodes. The fluorescence quenching isminimal, and Alexa Fluor® 647 can be analyzed in the sameinstrument channel as APC.
Please visit www.bdbiosciences.com/colors for additional fluorochromes to meet your multicolor analysis needs.
PE-Cy7/Alexa Fluor® 647
Alexa Fluor® 488/PE-Cy7
PE-Cy7/PE
Multicolor Analysis Data
4 www.bdbiosciences.comUnless otherwise specified, all products are for Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale.
10000
1000
100
10
1100001000100101
Anti-mouse CD3e APC-Cy7 (145-2C11)
Mo
use
CD
4 PE
(R
M 4
-5)
Mo
use
CD
8a P
E (5
3-6.
7)
10000
1000
100
10
1100001000100101
Isotype Control APC-Cy7 (A19-3)
Mo
use
CD
4 PE
(R
M 4
-5)
Mo
use
CD
8a P
E (5
3-6.
7)65.765.7
34.3
65.7
0.014
0
100006.496.49
67.767.7
5.395.39
20.420.4
6.49
67.7
5.39
20.4
1000
100
10
1100001000100101
Human CD8 PE (RPA-T8)
Hu
man
IFN
-γ P
E-C
y7 (
B27
)
100000.010.01
73.873.8
0.070.07
26.126.1
0.01
73.8
0.07
26.1
1000
100
10
1100001000100101
Human CD8 PE (RPA-T8)
Iso
typ
e C
on
tro
l PE-
Cy7
(M
OPC
-21)
100000.050.05
7171
0.050.050.05
71
0.05
28.9
1000
100
10
1100001000100101
Human CD8 PE-Cy7 (RPA-T8)
Iso
typ
e C
on
tro
l A
lexa
Flu
or®
488
(M
OPC
-21)
100003.29
68.468.468.4
3.52
24.7
1000
100
10
1100001000100101
Human CD8 PE-Cy7 (RPA-T8)
Hu
man
IFN
-γ
Ale
xa F
luo
r® 4
88 (
B27
)
100000.11
6.14
0.38
93.4
1000
100
10
1100001000100101
Mouse CD4 PE-Cy7 (RM4-5)
Iso
typ
e C
on
tro
lA
lexa
Flu
or®
647
(R
3-34
)
100001.14
5.17
24
69.7
1000
100
10
1100001000100101
Mouse CD4 PE-Cy7 (RM4-5)
Mo
use
IL-4
A
lexa
Flu
or®
647
(11
B11
)
Figure 2. Expression of IFN-γ by stimulated human peripheral blood lym-phocytes. Human PBMCs were stimulated for 4 hrs with PMA (5 ng/ml,Sigma, Cat. No. P-8139) and Ionomycin (500 ng, Sigma, P-8139) in the pres-ence of Brefeldin A (BD GolgiPlug™, Cat. No. 555029). Cells were har-vested, fixed, permeabilized, and stained with anti-human CD8-PE (Cat. No.555367) and either anti-human IFN–γ-PE-Cy7 antibody (Cat. No. 557643)(Panel A), or immunoglobulin isotype control-PE-Cy7 (Cat. No. 557646)(Panel B). (Donor 100)
Figure 3. Expression of IFN-γ by stimulated human peripheral bloodlymphocytes. Human PBMCs were stimulated for 4 hrs with PMA (5 ng/ml,Sigma, Cat. No. P-8139) and Ionomycin (500 ng, Sigma, P-8139) in thepresence of Brefeldin A (BD GolgiPlug™, Cat. No. 555029). Cells wereharvested, fixed, permeabilized, and stained with anti-human CD8-PE-Cy7 (Cat. No. 557746) and either anti-human IFN–γ Alexa Fluor® 488 (Cat.No. 557718) (Panel A), or immunoglobulin isotype control-Alexa Fluor®488 (Cat. No. 557702) (Panel B). (Donor 200)
Figure 4. Expression of IL-4 by stimulated CD4+ BALB/c spleen cells.Splenocytes from BALB/c mice were enriched for CD4+ cells by positiveselection using anti-CD4 coated plates (GK1.5, Cat. No. 553726) for 1 hrat 4°C. Cells were harvested and stimulated with plate-bound anti-mouseCD3e (145-2C11, Cat. No. 553057) and soluble anti-CD28 (37.51, Cat. No.553294) antibody in the presence of recombinant mouse IL-2 (Cat. No.550069) and IL-4 (Cat. No. 550067) for 2 days. The cells were subse-quently washed and expanded in IL-2 and IL-4 for 3 days. Followingexpansion, the cells were washed and re-stimulated for 4 hrs with PMA(Sigma, Cat. No. P-8139) and Ionomycin (Sigma, Cat. No. P-8139) in thepresence of Brefeldin A (BD GolgiPlug™, Cat. No. 555029). Followingincubation, the cells were harvested and stained with anti-mouse CD4-PE-Cy7 (Cat. No. 552775) and either anti-mouse IL–4-Alexa Fluor® 647(Cat. No. 557739) (Panel A) or immunoglobulin isotype control AlexaFluor® 647 (Cat. No. 557731) (Panel B).
Figure 1. Expression of CD3e-APC-Cy7 on mouse splenic lymphocytes.Mouse spleen cells were stained with both anti-mouse CD4-PE (RM4-5,Cat. No. 553048) and anti-mouse CD8-PE (53-6.7, Cat. No. 553032), along with either anti-mouse CD3e-APC-Cy7 (145-2C11, Cat. No. 557596)(Panel A), or immunoglobulin isotype control-APC-Cy7 (A19-3, Cat. No.557662) (Panel B).
A. B.
A. B.
A. B.
APC-Cy7/PEA. B.
APC-Cy7/APC
Please visit www.bdbiosciences.com/colors for fluorochrome spectra and fluorochrome combinations for typical flow cytometer instrument configurations.
Also Available
5Unless otherwise specified, all products are for Research Use Only.Not for use in diagnostic or therapeutic procedures. Not for resale.
100000
26.926.926.9
0.03
73
1000
100
10
1100001000100101
Human CD3 APC (UCHT1)
Iso
typ
e C
on
tro
l A
PC-C
y7 (
G15
5-17
8)
100003.89
21.121.121.1
12.9
62.1
1000
100
10
1100001000100101
Human CD3 APC (UCHT1)
Hu
man
CD
195
APC
-Cy7
(2
D7/
CC
R5)
Figure 5. Expression of CD195 (CCR5) on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stainedwith APC-conjugated mouse anti-human CD3 (UCHT1, Cat. No. 555335)and either APC-Cy7-conjugated mouse anti-human CD195 (2D7/CCR5,Cat. No. 557755) (Panel A) or an APC-Cy7-conjugated mouseimmunoglobulin isotype control (MOPC-21, Cat. No. 557873) (Panel B).
Figure 6. Multicolor analysis of human peripheral blood mononuclearcells stained with anti-human-CD3, anti-human-CD4, and anti-phospho-p44/42 (T202/Y204) Alexa Fluor® 647. PBMCs were depleted of adherentcells and stimulated by CD3/CD28 crosslinking (15 min at 4°C) orPMA/Ionomycin treatment (500 ng/ml for 30 min at 37°C). Followingtreatment, cells were washed, fixed, permeabilized, and stained with allthree antibodies simultaneously. Phospho-p44/42 (T202/Y204) was up-reg-ulated in only the CD3+/CD4+ double positive cells.
Data provided by Omar Perez PhD, Nolan Laboratory, Stanford University.
References:
1. Roederer, M., A.B. Kantor, D.R. Parks, and L.A. Herzenberg. Cy7PE and Cy7APC: Bright new probes for immunofluorescence. Cytometry 24: 191 – 197. 1996.
2. Beavis, A.J., and K.J. Pennline. ALLO-7: A new fluorescent tandem dye for use in flow cytometry. Cytometry 24:390 – 394. 1996.
3. Ingrid Schmid, M. D. Dagarag, M. Hausner, J. L. Matud, T. Just, R. B. Effros, and B. D. Jamieson. Simultaneous Flow Cytometric Analysis of Two Cell SurfaceMarkers, Telomere Length, and DNA Content. Cytometry 49:96 – 105. 2002
4. William G. Telford, W. G. Cox,and V. L. Singer. Detection of Endogenous and Antibody-Conjugated Alkaline Phosphatase With ELF-97 Phosphate in MulticolorFlow Cytometry Applications. Cytometry 43:117 – 125. 2001
• RUO (GMP) or ASR PE-Cy7 and APC-Cy7 conjugates and custom conjugation
Visit www.bdbiosciences.com for more information.
A. B.
ControlControlCD3/CD28 Cross Link
PMA/ionomycin
104
3.34
47.747.747.7
23.4
25.6
103
102
101
100
104103102101100
104103102101100 104103102101100
Human CD4 FITC (RPA-T4)
Hu
man
CD
3 Pe
rCP
(SK
7)
Cel
l Nu
mb
er80
100
60
40
20
0
80
100
60
40
20
0
Phospho-p44/42 (ERK1/2) (T202/Y204)Alexa Fluor® 647 (20A)
Alexa Fluor® 647/FITC/PerCP
6 www.bdbiosciences.comUnless otherwise specified, all products are for Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale.
Applications: FCM: Flow CytometryReactivities: Bab: Baboon; Cyno: Cynomolgus; Hu: Human; Ms: Mouse; Rhe: Rhesus
CD3 Bab, Cyno, Rhe SP34-2 FCM APC-Cy7 100 tests 557757
FCM PE-Cy7 100 tests 557749
CD8 Bab, Cyno, Rhe RPA-T8 FCM APC-Cy7 100 tests 557760
FCM PE-Cy7 100 tests 557750
Antibodies to Non-Human Primate Cell Surface Markers
DESCRIPTION REACT CLONE APPS FORMAT SIZE CAT. NO.
Mouse IgG1, κ MOPC-21 FCM PE-Cy7 100 tests 557872
FCM APC-Cy7 100 tests 557873
Mouse IgG2a, κ G155-178 FCM APC-Cy7 100 tests 557751
Isotype Controls for use with Antibodies to Human Cell Surface Markers
DESCRIPTION REACT CLONE APPS FORMAT SIZE CAT. NO.
CD195/CCR5 Hu 2D7/CCR5 FCM APC-Cy7 100 tests 557755
FCM PE-Cy7 100 tests 557752
CCR7 Hu 3D12 FCM Alexa Fluor® 488 100 tests 557723
FCM Alexa Fluor® 647 100 tests 557734
FCM PE-Cy7 100 tests 557648
Antibodies to Human Cytokines and Chemokine Receptors
DESCRIPTION REACT CLONE APPS FORMAT SIZE CAT. NO.
CD3 Hu SK7 FCM PE-Cy7 100 tests 557851
FCM APC-Cy7 100 tests 557832
CD4 Hu SK3 FCM PE-Cy7 100 tests 557852
RPA-T4 FCM APC-Cy7 100 tests 557871
CD8 Hu RPA-T8 FCM PE-Cy7 100 tests 557746
SK1 FCM APC-Cy7 100 tests 557834
CD11b/Mac-1 Hu ICRF44 FCM PE-Cy7 100 tests 557743
FCM APC-Cy7 100 tests 557754
CD14 Hu M5E2 FCM PE-Cy7 100 tests 557742
MφP9 FCM APC-Cy7 100 tests 557831
CD16 Hu 3G8 FCM APC-Cy7 100 tests 557758
FCM PE-Cy7 100 tests 557744
CD19 Hu SJ25C1 FCM PE-Cy7 100 tests 557835
FCM APC-Cy7 100 tests 557791
CD25 Hu M-A251 FCM APC-Cy7 100 tests 557753
FCM PE-Cy7 100 tests 557741
CD45 Hu HI30 FCM PE-Cy7 100 tests 557748
2D1 FCM APC-Cy7 100 tests 557833
CD56 Hu B159 FCM PE-Cy7 100 tests 557747
CD69 Hu FN50 FCM APC-Cy7 100 tests 557756
FCM PE-Cy7 100 tests 557745
Antibodies to Human Cell Surface Markers
DESCRIPTION REACT CLONE APPS FORMAT SIZE CAT. NO.
New Fluorochrome Reagents for Immunology and Cell Signaling
7Unless otherwise specified, all products are for Research Use Only.Not for use in diagnostic or therapeutic procedures. Not for resale.
Applications: FCM: Flow CytometryReactivities: Bab: Baboon; Cyno: Cynomolgus; Hu: Human; Ms: Mouse; Rhe: Rhesus
IFN-γ Hu B27 FCM Alexa Fluor® 488 100 tests 557718
FCM Alexa Fluor® 647 100 tests 557729
FCM PE-Cy7 100 tests 557643
IL-4 Hu MP4-25D2 FCM Alexa Fluor® 488 100 tests 557727
FCM Alexa Fluor® 647 100 tests 557738
TNF Hu MAb11 FCM Alexa Fluor® 488 100 tests 557722
FCM Alexa Fluor® 647 100 tests 557733
FCM PE-Cy7 100 tests 557647
Antibodies to Human Cytokines and Chemokine Receptors (continued)
DESCRIPTION REACT CLONE APPS FORMAT SIZE CAT. NO.
Mouse IgG1, κ MOPC-21 FCM Alexa Fluor® 488 100 tests 557721
FCM Alexa Fluor® 647 100 tests 557732
FCM PE-Cy7 100 tests 557646
Isotype Controls for use with Antibodies to Human Cytokines and Chemokine Receptors
DESCRIPTION REACT CLONE APPS FORMAT SIZE CAT. NO.
CD3 e (CD3 ε chain) Ms 145-2C11 FCM APC-Cy7 0.1 mg 557596
FCM PE-Cy7 0.1 mg 552774
CD4 (L3T4) Ms GK1.5 FCM APC-Cy7 0.1 mg 552051
RM4-5 FCM PE-Cy7 0.1 mg 552775
CD8a (Ly-2) Ms 53-6.7 FCM APC-Cy7 0.1 mg 557654
FCM PE-Cy7 0.1 mg 552877
CD11b (Integrin αm chain) Ms M1/70 FCM APC-Cy7 0.1 mg 557657
FCM PE-Cy7 0.1 mg 552850
CD19 Ms 1D3 FCM APC-Cy7 0.1 mg 557655
FCM PE-Cy7 0.1 mg 552854
CD25 (IL-2 Receptor α chain) Ms PC61 FCM APC-Cy7 0.1 mg 557658
FCM PE-Cy7 0.1 mg 552880
CD45 (LCA, Ly-5) Ms 30-F11 FCM APC-Cy7 0.1 mg 557659
FCM PE-Cy7 0.1 mg 552848
CD45R/B220 Ms RA3-6B2 FCM APC-Cy7 0.1 mg 552094
FCM PE-Cy7 0.1 mg 552772
FCM PE-Texas Red 0.1 mg 551489
CD49b (Integrin α2 chain) Ms HMα2 FCM APC-Cy7 0.1 mg 557656
CD69 (Very Early Activation Ag) Ms H1.2F3 FCM PE-Cy7 0.1 mg 552879
CD95 (Fas) Ms Jo2 FCM PE-Cy7 0.1 mg 557653
IgM Ms R6-60.2 FCM PE-Cy7 0.1 mg 552867
Ly-6G and Ly-6C (Gr-1) Ms RB6-8C5 FCM PE-Cy7 0.1 mg 552985
NK-1.1 (NKR-P1B & NKR-P1C) Ms PK136 FCM PE-Cy7 0.1 mg 552878
Antibodies to Mouse Cell Surface Markers
DESCRIPTION REACT CLONE APPS FORMAT SIZE CAT. NO.
Applications: FCM: Flow CytometryReactivities: Bab: Baboon; Cyno: Cynomolgus; Hu: Human; Ms: Mouse; Rhe: Rhesus
Hamster IgG1, κ (anti-TNP) A19-3 FCM APC-Cy7 0.1 mg 557662
FCM PE-Cy7 0.1 mg 552811
Hamster IgG2, λ Ha4/8 FCM APC-Cy7 0.1 mg 557664
FCM PE-Cy7 0.1 mg 557652
Rat IgG1, λ (anti-KLH) A110-1 FCM APC-Cy7 0.1 mg 557663
FCM PE-Cy7 0.1 mg 552869
Rat IgG2a, κ R35-95 FCM APC-Cy7 0.1 mg 552770
FCM PE-Cy7 0.1 mg 552784
Rat IgG2b, κ A95-1 FCM APC-Cy7 0.1 mg 552773
FCM PE-Cy7 0.1 mg 552849
Mouse IgG2a, κ G155-178 FCM PE-Cy7 0.1 mg 552868
Isotype Controls for use with Antibodies to Mouse Cell Surface Markers
DESCRIPTION REACT CLONE APPS FORMAT SIZE CAT. NO.
IFN-γ Ms XMG1.2 FCM Alexa Fluor® 488 0.1 mg 557724
FCM Alexa Fluor® 647 0.1 mg 557735
FCM PE-Cy7 0.1 mg 557649
IL-2 Ms JES6-5H4 FCM Alexa Fluor® 488 0.1 mg 557725
FCM Alexa Fluor® 647 0.1 mg 557736
IL-4 Ms 11B11 FCM Alexa Fluor® 488 0.1 mg 557728
FCM Alexa Fluor® 647 0.1 mg 557739
TNF Ms MP6-XT22 FCM Alexa Fluor® 488 0.1 mg 557719
FCM Alexa Fluor® 647 0.1 mg 557730
FCM PE-Cy7 0.1 mg 557644
Antibodies to Mouse Cytokines
DESCRIPTION REACT CLONE APPS FORMAT SIZE CAT. NO.
Rat IgG1, κ R3-34 FCM Alexa Fluor® 488 0.1 mg 557720
FCM Alexa Fluor® 647 0.1 mg 557731
FCM PE-Cy7 0.1 mg 557645
Rat IgG2b, κ A95-1 FCM Alexa Fluor® 488 0.1 mg 557726
FCM Alexa Fluor® 647 0.1 mg 557737
FCM PE-Cy7 0.1 mg 557651
Isotype Controls for use with Antibodies to Mouse Cytokines
DESCRIPTION REACT CLONE APPS FORMAT SIZE CAT. NO.
New Fluorochrome Reagents for Immunology and Cell Signaling (continued)
8 www.bdbiosciences.comUnless otherwise specified, all products are for Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale.
9Unless otherwise specified, all products are for Research Use Only.Not for use in diagnostic or therapeutic procedures. Not for resale.
Applications: FCM: Flow CytometryReactivities: Bab: Baboon; Cyno: Cynomolgus; Hu: Human; Ms: Mouse; Rhe: Rhesus
Caveolin1 (pY14) Hu, Ms, Rat 56 FCM PE 50 tests 612568
ERK1/2 (pT202/pY204) Hu, Ms, Rat 20A FCM Alexa Fluor® 488 50 tests 612592
ERK1/2 (pT202/pY204) Hu, Ms, Rat 20A FCM Alexa Fluor® 647 50 tests 612593
ERK1/2 (pT202/pY204) Hu, Ms, Rat 20A FCM PE 50 tests 612566
p38 MAPK (pT180/pY182) Hu, Ms, Rat 36 FCM Alexa Fluor® 488 50 tests 612594
p38 MAPK (pT180/pY182) Hu, Ms, Rat 36 FCM Alexa Fluor® 647 50 tests 612595
p38 MAPK (pT180/pY182) Hu, Ms, Rat 36 FCM PE 50 tests 612565
Lck (pY505) Hu, Ms, Rat 4 FCM PE 50 tests inquire
Stat1 (pY701) Hu, Ms 4A FCM Alexa Fluor® 488 50 tests 612596
Stat1 (pY701) Hu, Ms 4A FCM Alexa Fluor® 647 50 tests 612597
Stat1 (pY701) Hu, Ms 4A FCM PE 50 tests 612564
Stat3 (pY705) Hu, Ms 4 FCM Alexa Fluor® 488 50 tests inquire
Stat3 (pY705) Hu, Ms 4 FCM Alexa Fluor® 647 50 tests inquire
Stat3 (pY705) Hu, Ms 4 FCM PE 50 tests 612569
Stat5 (pY694) Hu 47 FCM Alexa Fluor® 488 50 tests 612598
Stat5 (pY694) Hu 47 FCM Alexa Fluor® 647 50 tests 612599
Stat5 (pY694) Hu 47 FCM PE 50 tests 612567
Stat6 (pY641) Hu 18 FCM Alexa Fluor® 488 50 tests 612600
Stat6 (pY641) Hu 18 FCM Alexa Fluor® 647 50 tests 612601
Stat6 (pY641) Hu 18 FCM PE 50 tests 612701
BD™ PhosFlow Antibody Reagents
DESCRIPTION REACT CLONE APPS FORMAT SIZE CAT. NO.
Streptavidin FCM APC-Cy7 0.1 mg 554063
FCM PE-Cy7 0.1 mg 557598
FCM PE-Texas Red 0.1 mg 551487
Second-Step Reagents
DESCRIPTION REACT CLONE APPS FORMAT SIZE CAT. NO.
Visit www.bdbiosciences.com/colors for an updated product listing of Alexa Fluor® conjugated antibodies to mouse, human, and non-human primate cell surface markers, and more.
BD™ Phospho-Specific Solutions
Introducing BD™ PhosFlow Reagents
Direct fluorescent conjugates for multiparameter, phospho-specific flow cytometry.Now the company synonymous with flowcytometry brings you the finest set of anti-body conjugates for the study of proteinphosphorylation.
BD Biosciences Pharmingen is developingan extensive line of phospho-specific antibodies for multi-color flow cytometry.Featuring the high quality BD TransductionLaboratories™ brand of antibodies, conju-gated to Alexa Fluor® dyes and PE, thesereagents allow you to study severalparameters simultaneously in 3-, 4-, or even 6-color experiments.
• Study multiple phosphorylationevents in a single experiment
• Evaluate the effects of proteinphosphorylation in complex cellpopulations
• Monitor intracellular kinases inconjugation with surface markers
• Study the phosphorylation ofERK1/2, p38 MAPK, Stats 1, 3, 5,and 6, and more
To get the most out of your studies ofphosphorylation, visit www.bdphosflow.com
BD Biosciences Pharmingenwww.bdbiosciences.com
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BD™ Instrument and Reagent Solutions
Explore the Future of BD FACS™ SystemsBD FACSAria™ Cell Sorter
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Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.* Patents — PE and APC: US 4,520,110; 4,859,582; 5,055,556; Europe 76,695; Canada 1,179,942
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