new technology and workflow for integrated collection, stabilization and purification of circulating...

2

PAXgene® Tissue System (RUO)Two worlds in one sample

PAXgene is a tradmark of PreAnalytiX GmbH. All other brands are trademarks of theire respective owners. © 2010-2016 PreAnalytiX.

01.05.2023

New workflows & applicationsDr. Tomasz Krenz

01.05.2023 3

PAXgene Tissue System* (RUO) Agenda

About PreAnalytiXPAXgene Tissue SystemWorkflow 1: PF-tissueWorkflow 2: PFPE-tissueWorkflow 3: PFCE-tissueLaser capture microdissectionReferences / Web resources

*The PAXgene Tissue applications presented here are for research use only. Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease

01.05.2023 4

About PreAnalytiX GmbHA QIAGEN/BD Joint Venture since 1998

BD• Specimen collection & stabilization

• Storage & transport

QIAGEN• Isolation of nucleic acids & other

biomolecules

• Automation

• Analysis

01.05.2023 5

PreAnalytiX GmbHOur Mission

Preanalytical errors cost ~ $460,000 / year in a typical German hospital Frost & Sullivan, 2011 survey on behalf of BD

Postanalytics

Preanalytics

68%

13%

19%

Analytics

“Preanalytical errors still account for nearly 60–70% of all problems occurring in laboratory diagnostics, … attributable to mishandling procedures during collection, handling, preparing or storing the specimens.” Lippi, G. et al. (2011) Preanalytical quality improvement: from dream to reality. Clin Chem Lab Med 49, 1113−1126. Epub 2011 Apr 25.

01.05.2023 6




01.05.2023 7

Morphology Nucleic Acid Preservation Protein Preservation

PAXgene Tissue System √ √ √Competitor A

Morphology destroyed or compromised √ -

Competitor B Morphology destroyed or compromised

√ √

Formalin √ Crosslinking of biomolecules;Lack of standardization; no stabilization of biomolecules

PAXgene Tissue: Product PositioningSimultaneous preservation of morphology and biomolecules

PAXgene Tissue enables combined histological and multimodal analysis of biomolecules from the same sample

01.05.2023 8

PAXgene Tissue SystemSystem components

Collection, fixation and stabilizationPAXgene Tissue Container (10) containing PAXgene Tissue FIX and STABILIZER

dual chamber: 1 histocassette

PAXgene Tissue FIX Container (10 x 50 ml) + PAXgene Tissue STABILIZER concentrate

(8 x 150 ml for 4 liters of STABILIZER reagent each) single chamber: up to 4 histocassettes or larger tissue

sample

+

RNA RNA & miRNA DNA

Protein

Qproteome FFPE Tissue KitPAXgene Tissue Kits

Extraction

01.05.2023 9




01.05.2023 10

AnalysisBiomolecule purification

Storage at −20 to −80°C

PAXgene Tissue: Workflows1. PF-Tissue

Tissue fixation, stabilization & transport

Tissue resection

Fixation2–48 hours (depending on tissue size)

Stabilization & storageUp to 7 days at RTUp to 4 weeks at 2–8°CUp to 5 years at –20°C (studies ongoing)Up to 6 years at –80°C (studies ongoing)

Biomolecule purificationPAXgene Tissue RNA kitPAXgene Tissue miRNA kitPAXgene Tissue DNA kitQproteome® FFPE kit & supplementary protocol

PF-Tissue

≤ 15 x 15 x 4 mm

PAXgene TissueContainer

PAXgene TissueFIX Container (50ml)

Cryo Vial

RNA, DNA, Protein

01.05.2023 11

1. PF-Tissue WorkflowHigh-quality NA from different tissue types including fibrous or fatty tissues

Φ RIN

- 21kb

RNA

DNA

01.05.2023 12




01.05.2023 13

AnalysisBiomolecule purification& Staining

LaserCaptureMicrodisection

SectioningProcessing & embedding

Storage at -20 to -80°C


Paraffin-Embedding

PFPE-Tissue

RNA DNA Protein

H&E IHC ISH

PF-Tissue



Microtome

Cryo Vial

LCM

Processing and paraffin embeddingPosition #1 of tissue processor: STABILIZER or ≥ 80% EtOHLow melting point paraffin (m.p. 54°C)Inkubation in paraffin < 3 hours

StainingHistochemical (H&E, PAS, …)ImmunohistochemicalIn situ hybridization

PAXgene Tissue: Workflows2. PFPE-Tissue

Biomolecule purificationPAXgene Tissue RNA kitPAXgene Tissue miRNA kitPAXgene Tissue DNA kitQproteome® FFPE kit & supplementary protocol

01.05.2023 14

2. PFPE-Tissue WorkflowPreservation of tissue morphology

Liver Spleen Kidney Intestine Lung Brain

H&E staining

01.05.2023 15

2. PFPE-Tissue WorkflowIHC & ISH with clinical samples

PFPE

FFPE

IHC of human tonsil:

Ki-67, clone MIB-1

ISH of human breast cancer: HER2 FISH

01.05.2023 16

2. PFPE-Tissue WorkflowEvaluation of RNA quality with clinical samples

Viertler et al. (2012) J Mol Diagn 14, 458.

RNA integrity from human liver:

PFPE vs. FFPE vs. snap frozen

Gene expression from human liver:qRT-PCR on a TaqMan array plate92 cancer pathway genes PFPE: Strong correlation (R² = 0.99)

to snap-frozen FFPE: large gene-to-gene variation (R² = 0.81)

01.05.2023 17

2. PFPE-Tissue WorkflowEvaluation of DNA quality with clinical samples

DNA integrity from human colorectal cancer:

PFPE: High molecular mass, suited for multiplex and long-range PCRFFPE: No recovery of large DNA molecules

Viertler et al. (2012) J Mol Diagn 14, 458.

01.05.2023 18

2. PFPE-Tissue WorkflowAnalytical protein techniques

Reverse-phase protein arrays

Western blot

Maldi-imaging mass spectrometry

2D-PAGE

ELISA (denatured proteins)

Ergin, B. et al. (2010) J Proteome Res 9, 5188−5196.

Ergi, B. et al. (2010)

Gündisch, S. et al.(2013) PLoS One 8, e60638.

Gündisch, S. et al. (2013)

Gündisch, S. et al. (2013)

01.05.2023 19

PAXgene-Tissue WorkflowAutomated purification of genomic DNA and RNA from PF & PFPE

QIAcube protocols available: significant savings of hands-on time

RNA from sections of PFPE tissueRNA from PF tissueDNA from section of PFPE tissueDNA from PF Tissue

01.05.2023 20




01.05.2023 21

AnalysisBiomolecule purification& staining

LaserCaptureMicrodissection

SectioningProcessing & embedding

Storage at −20 to −80°C

PAXgene Tissue: New WorkflowsPFCE: PAXgene fixed, cryo-embedded


Cryo-Embedding

PFCE-Tissue

RNA DNA Protein

PF-Tissue



Cryotome

Cryo Vial

LCM

Processing and cryo embeddingWash in 30% [w/v] sucrose at 2-8°COverlay Tissue with cryo-embedding media (e.g. Leica FCS22)Freeze in pre-chilled Isopentane or liquid N2

H&E IHC ISH

Biomolecule purification and Staining

Supplementary Protocols

01.05.2023 22

3. PFCE-Tissue WorkflowA. Preparation in isopentane on dry ice

< 2 x 15 x 15 mm

1. Tissue fixation and sucrose incubationTissue resection Tissue fixation &

stabilizationON incubation in 30% [w/v] sucrose

Freezing in isopentanecooled on dry ice

2. Snap-freezing in isopentane and embedding in cryo-media

Frozen tissue added to cryomold filled with cryo-embedding medium

Specimen disk placed on top

Cryomold submerged into isopentane

PFCE specimen

01.05.2023 23

< 2 x 15 x 15 mm

1. Tissue fixation and sucrose incubation Tissue resection Tissue fixation &

stabilizationON incubation in 30% [w/v] sucrose

2. Embedding in cryo-media and snap-freezing in liquid nitrogen

Tissue placed in cryomold filledwith cryo-embedding media

Specimen disk Placed on top

PFCE specimen

Snap freezing in liquid nitrogen

3. PFCE-Tissue WorkflowB. Preparation with liquid nitrogen

01.05.2023 24

3. PFCE-Tissue WorkflowKits & Protocols

Object PAXgene Tissue Supplementary Protocols Kits / ProductsWorkflow -Preparation of PFCE samples

Cryo-embedding tissue specimens fixed and stabilized with the PAXgene® Tissue System

PAXgene Tissue ContainerPAXgene Tissue FIX Container + Stabilizer

DNAPurification of genomic DNA from sections ofPAXgene® Tissue fixed, cryo-embedded (PFCE)tissue

PAXgene Tissue DNA Kit

RNAPurification of total RNA from sections ofPAXgene® Tissue fixed, cryo-embedded (PFCE)tissue

PAXgene Tissue RNA Kit

miRNAPurification of total RNA, including miRNA, from sections of PAXgene® Tissue fixed, cryo-embedded (PFCE) tissue

PAXgene Tissue miRNA Kit

ProteinPurification of full-length proteins from sections of PAXgene® Tissue fixed, cryo-embedded (PFCE) tissue

Qproteome FFPE Kit

25Date Text, Location

Liver Spleen

Heart muscleIntestine

3. PFCE-Tissue WorkflowHistomorphology

H&E staining

01.05.2023 26

3. PFCE-Tissue WorkflowHigh-quality NA from different tissue types

RIN: 8.5 RIN: 7.6RIN: 8.9 RIN: 9.1RIN: 8.6

RNA

- 21kb

DNA

Liver Spleen Intestine

Heart muscle

Lung

01.05.2023 27

Data kindly provided by Karl-Friedrich Becker, Technical University of Munich, Germany

Liver StomachSection of PFCE tissue

mounted on slide

Cryo-medium removed

Area of interest removed manually or by laser microdissection

Collected into Qproteome FFPE buffer EXB Plus

Incubated for 10 min @95°C

3. PFCE-Tissue WorkflowFull-length (phospho-)proteins from PFCE tissue

SDS-PAGE & western blot

12.5 µg protein per lane

01.05.2023 28




01.05.2023 29

BiomoleculePurificationLaser Capture MicrodissectionStaining (H&E)

Sectioning/ removal of embedding medium

Processing & embedding

Paraffin-Embedding

Cryo-Embedding

PFPE-Tissue

PFCE-Tissue

Microtome

Cryotome

RNA DNALMD (e.g. Leica 6500)H&E

PFPE workflow

PFCE workflow

PAXgene Tissue: New WorkflowsLaser Capture Microdissection (LCM)

01.05.2023 30

MicrodissectionSet Up

4. Laser Capture Microdissection (LCM)Procedure

Sample Processing

Slidepreparation

PFPE/PFCE tissue sections Microscope + Sample

LCM Software

Collection tubesstain

Definition of region

Cutting

Collection

Collection in Lysis buffer

Purification of genomic DNA Purification of total RNAPurification of total RNA, including miRNA

P1000877.MP4

01.05.2023 31

Object PAXgene Tissue Supplementary Protocols Kits / Products

Workflow -Preparation of slides

Preparation of sections from PAXgene® Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues for manual or laser microdissection (LMD)

PAXgene Tissue ContainerPAXgene Tissue FIX Container + Stabilizer

DNAPurification of genomic DNA from microdissected PAXgene® Tissue-fixed, paraffin-embedded (PFPE) and PAXgene Tissue-fixed, cryo-embedded (PFCE) tissues

PAXgene Tissue DNA Kit

RNAPurification of total RNA from microdissected PAXgene® Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues

PAXgene Tissue RNA Kit

miRNAPurification of total RNA, including miRNA, from microdissected PAXgene® Tissue fixed, paraffin embedded (PFPE) and PAXgene Tissue fixed, cryoembedded (PFCE) tissues

PAXgene Tissue miRNA Kit

4. Laser Capture Microdissection (LCM)Kits & Protocols

01.05.2023 32

liver

sple

en

colo

n

liver

sple

en

colo

n

liver

sple

en

colo

n

PFPE PFCE FFPE[nt] High-quality and high-molecular−weight RNA Agilent Bioanalyzer: Gel and corresponding RIN values of total RNA from an area of 1 mm² (4,000−12,000 cells) ß-act RT PCR

4. Laser Capture Microdissection (LCM)RNA from PFPE & PFCE tissue

# cells

01.05.2023 33

PFPE PFCEliv

er

sple

en

colo

n

liver

sple

en

colo

n

High-quality miRNA Agilent Bioanalyzer Small RNA: Gel with calculated miRNA content and ElectropherogramsArea of 1 mm² (4,000−12,000 cells) selected

[nt]

4. Laser Capture Microdissection (LCM)miRNA from PFPE & PFCE tissue

01.05.2023 34

Total yield and performance (ß-act PCR)Area of 0.005−1 mm² (20−12,000 cells) selected

4. Laser Capture Microdissection (LCM)DNA from PFPE & PFCE tissue

01.05.2023 35




01.05.2023 36

ReferencesProjects / Collaborations

SPIDIA

STRATFix UK

Genotype Tissue Expression Project (GTEx)

CBmed Austria

Barret Syndrome Project

Genesis

Enabling stratified medicine with novel fixatives for improved pre-analytical pathology workflows

Next generation sequencing in molecular pathology diagnostics

Identification of Biomarkers for the Development of Adenocarcinoma from Barrett-Esophagus

Genetic Biopsy for Prediction of Surveilance Intervals after Endoscopic Resection of Colonic Polyps

Standardization and Improvement of Generic Pre-analytical Tools and Procedures for In Vitro Diagnostics

7 research organizations, 8 companies, 1 standards organization (CEN), Coordinated by QIAGEN

8 clinical sides, 1 company, Coordinated by QIAGEN

Establish a resource database and tissue bank to study the relationship between genetic variation and gene expression

Over 700 donors, >21,000 collected tissue samples

Up to 2000 patients

220 samples, 100 patients; follow up project with ~10.000 patients

01.05.2023 37

ReferencesScientific articles in peer-reviewed journals

Author Titel Journal ContentYamaguchi, et al. Comprehensive DNA Methylation and Extensive Mutation Analyses of HER2-Positive Breast

Cancer.Oncology. 2015 Jan; [Epub ahead of print]

409 cancer-related genes/ bench-top NGS/ DNA methylation statuses/ bead array with 485,512 probes

Keen J and Moore H The Genotype-Tissue Expression (GTEx) project: Linking Clinical Data with Molecular Analysis to Advance Personalized Medicine.

J.Pers. Med. 2015 Feb;5:22-29

Whole genome and RNA sequencing

Andersen GB, et al. Improved reproducibility in genome-wide DNA methylation analysis for PAXgene® fixed samples compared to restored FFPE DNA.

Anal Biochem. 2014 Sep; 468C:50-58. Genome-wide DNA methylation analysis/ Illumina 450K BeadChip

Gündisch S, et al. Evaluation of colon cancer histomorphology: a comparison between formalin and PAXgene tissue fixation by an international ring trial.

Virchows Arch. 2014 Nov; 465(5):509-19.

Blinded, randomized ring trial by 20 pathologists; virtual microscopy; histological grading and quality parameter

Kap M, et al. Inactivation of Influenza A virus, Adenovirus, and Cytomegalovirus with PAXgene Tissue Fixative and Formalin.

Biopreservation and Biobanking. 2013 Aug; 11(4): 229-234

Viral inactivation characteristics by PAXgene Tissue fixative

Staff S, et al. Preservation of nucleic acids and tissue morphology in paraffin-embedded clinical samples: comparison of five molecular fixatives.

J Clin Pathol. 2013 Sep;66(9):807-10

Comparison study Z7, RCL2, PAXgene Tissue, Allprotect, RNAlater, Formlin; DNA, RNA, Morphology, IHC, FISH, CISH

Oetjen J, et al. MRI-compatible pipeline for three-dimensional MALDI imaging mass spectrometry using PAXgene fixation.

J Proteomics. 2013 Sep;90:52-60

Computational pipeline for molecular analysis combining 3D MALDI imaging, magnetic resonance imaging and H&E

Lonsdale, et al. The Genotype-Tissue Expression (GTEx) project. Nature Genetics 2013 Jun; 45, 580-585

Description of GTEx project

Gündisch S, et al. The PAXgene Tissue System Preserves Phosphoproteins in Human Tissue Specimens and Enables Comprehensive Protein Biomarker Research.

PLoS One. 2013;8(3):e60638

Proteome including phosphoproteome analysis; fresh frozen, PFPE, FFPE, western blotting, 2D-PAGE, ELISA

Belloni B, et al. Will PAXgene substitute formalin? A morphological and molecular comparative study using a new fixative system.

J Clin Pathol. 2013 Feb;66(2):124-35

Melanoma fresh frozen, PFPE, FFPE, morphology, IHC, DNA, RNA

Groelz D, et al. Non- Formalin Fixative versus Formalin-fixed Tissue: A Comparison of Histology and RNA Quality.

Exp Mol Pathol. 2013 Feb;94(1):188-94

Morphology and RNA preservation; comparison PFPE and FFPE; RT-qPCR

Viertler C, et al. A New Technology for Stabilization of Biomolecules in Tissues for Combined Histological and Molecular Analyses.

J Mol Diagn. 2012 Sep;14(5):458-66

RNA, DNA, miRNA preservation, fresh frozen, PFPE, FFPE, expression profiling

Trede D, et al. Exploring three-dimensional matrix-assisted laser desorption/ionization imaging mass spectrometry data: three-dimensional spatial segmentation of mouse kidney.

Anal Chem. 2012 Jul;84(14):6079-87.

3D MALDI imaging, 3D spatial segmentation

Baker M Biorepositories: Building better biobanks. Nature 2012 Jun;486(7401):141-6.

Efforts to improve biobanking

Kap M, et al. Histological Assessment of PAXgene Tissue Fixation and StabilizationReagents.

PLoS ONE. 2011;6(11)

Quality assessment PFPE vs FFPE, 20 different human tissue types, H&E, IHC, 4 pathologists, histological grading and quality

Ergin B, et al. Proteomic analysis of PAXgene-fixed tissues. J Proteome Res. 2010 Oct;9(10):5188-96

Proteome analysis from fresh frozen, PFPE and FFPE; western blotting, reverse-phase microarrays, MALDI imaging MS

Loibner et al. (2016) Pathogen Inactivating Properties and Increased Sensitivity in Molecular Diagnostics by PAXgene, a Novel Non-Crosslinking Tissue Fixative.

PLoS One. 2016 Mar 14;11(3)

Oberauner-Wappis et al. (2016)

Protocol for HER2 FISH determination on PAXgene-fixed and paraffin-embedded tissue in breast cancer

Accepted in International Journal of Experimental Pathology

Carithers et al. (2015)

A novel approach to high-quality postmortem tissue procurement: the GTEx project

Biopreserv Biobank. 2015 Oct;13(5):311-9.

Hara et al. (2015) Surgical Specimens of Colorectal Cancer Fixed with PAXgene Tissue System Preserve High-Quality RNA


Gillard, M. et al. (2015)

Next-gen tissue: preservation of molecular andmorphological fidelity in prostate tissue.

Am J Transl Res 7:1227-1235

Yamaguchi, T. et al. (2015)

Comprehensive DNA Methylation and Extensive Mutation Analyses of HER2-Positive Breast Cancer.

Oncology. 2015;88(6):377-84

Loibner et al. (2016) Pathogen Inactivating Properties and Increased Sensitivity in Molecular Diagnostics by PAXgene, a Novel Non-Crosslinking Tissue Fixative.

PLoS One. 2016 Mar 14;11(3)

Oberauner-Wappis et al. (2016)

Protocol for HER2 FISH determination on PAXgene-fixed and paraffin-embedded tissue in breast cancer

Accepted in International Journal of Experimental Pathology

Carithers et al. (2015)

A novel approach to high-quality postmortem tissue procurement: the GTEx project


Hara et al. (2015) Surgical Specimens of Colorectal Cancer Fixed with PAXgene Tissue System Preserve High-Quality RNA


Gillard, M. et al. (2015)

Next-gen tissue: preservation of molecular andmorphological fidelity in prostate tissue.

Am J Transl Res 7:1227-1235

Yamaguchi, T. et al. (2015)

Comprehensive DNA Methylation and Extensive Mutation Analyses of HER2-Positive Breast Cancer.

Oncology. 2015;88(6):377-84

01.05.2023 38

Web Resources Technical Support

Technical notesPI3K mutation analysisIHC assay optimization (Ki67)Formalin contamination during processingVacuum sealing for fixed and stabilized tissues for transportRNA yield, purity and integrity from PFPE rat tissueKRAS and BRAF mutational status using Pyrosequencing technology…

Supplementary protocolsManual processingDeparaffinization with Deparaffinization SolutionPurification of proteins from sections of PFPE tissueAllprep: Simultaneous purification of RNA and DNA from sections of PFPE tissuePurification of RNA/DNA from microdissected PFPE and PFCE tissue

IHC application list

Manual stainingBenchmark XTMicrowaveLeica Bond MaxDako Autostainer

OtherHandbooksPostersBrochuresList of references in peer reviewed journalsFAQ ListSafety data sheets

Constantly growing list of technical support: http://www.preanalytix.com/products/tissue/fixation-stabilization/paxgene-tissue-container

01.05.2023 39

Web ResourcesTissue Atlas

http://www.preanalytix.com/products/tissue/tissue-atlasHuman large intestine Rat retina Rat tongue

Rat kidney Human large intestine Human stomach

H&E

PAS

IHC

Human tonsil Human colorectal cancer Human breast cancer Human tonsil

Human liver

Rat skin sebaceous glands

http://www.preanalytix.com/products/tissue/tissue-atlas

40

Q&A session

01.05.2023

Thank you for your attention!

For up-to-date licensing information and product-specific disclaimers for QIAGEN products, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.com or www.preanalytix.com or can be requested from QIAGEN Technical Services or your local distributor.

41

Q&A session

01.05.2023

Supplement

For up-to-date licensing information and product-specific disclaimers for QIAGEN products, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.com or www.preanalytix.com or can be requested from QIAGEN Technical Services or your local distributor.

01.05.2023 42

1. PF-Tissue WorkflowFull-length (phospho-)proteins from PF tissue

Data kindly provided by Karl-Friedrich Becker, Technical University of Munich, Germany

12.5 µg protein per lane

Up to 100 mg of PF tissue washed with PBS

Disrupted and homogenized with TissueLyser

Incubated for 10 min @95°C

Collected into Qproteome FFPE buffer EXB Plus

Liver Stomach

SDS-PAGE & western blot

01.05.2023 43

ReferencesSPIDIA

Main FactsFor “Standardization and Improvement of Generic Pre-analytical Tools and Procedures for In Vitro Diagnostics”Funded by FP7-HEALTH (EC)7 public research organizations, 8 companies, 1 standards organization (CEN)Coordinated by QIAGENRun time from Oct. 2008 – March 2013

Main GoalsNew preanalytical tools & technologies (blood, plasma, tissue, swabs)Sample quality markers (blood, tissue)Pan-European guidelines for preanalytics (blood, tissue) Training and dissemination

01.05.2023 44

ReferencesThe Genotype Tissue Expression Project (GTEx)

NIH common fund initiativeAim: establish a resource database and tissue bank to study the relationship between genetic variation and gene expressionOver 700 donors (>21,000 tissue samples) have been collected to datePFPE-tissue for histological examinationRNA from PF-tissue for RNA sequencing

”PAXgene is at least as good as formalin for histology. It is now our only morphologic fixative.” OBBR pathologists Philip Branton, John Madden James Robb, Leslie Sobin, Carolyn Comptonhttp://www.genome.gov/Pages/About/NACHGR/September2011AgendaDocs/NACHGR_Sep122011_%20GTExUpdate_Struewing.pdf

“Each tissue is preserved in PAXgene® tissue fixative …sections are examined by a team of board certified pathologists …images and histological data will be available to researchers …PAXgene® tissue-fixed samples are then shipped to … for whole genome and RNA sequencing.”Keen, J.C. and Moore, H.M. (2015) The Genotype-Tissue Expression (GTEx) Project: Linking clinical data with molecular analysis to advance personalized medicine. J Pers Med 5, 22−29.

01.05.2023 45

ReferencesThe Barret Syndrome Project

endoscopic tissue retrieval

PAXgene Tissue fixation & stabilization,transport and processing

H&E, PAS – staining for diagnosisIHC stainingDNA, RNA purification

Whole genome amplificationNext-generation sequencing

m4 Cluster Munich fund initiative — collaboration with Technical University MunichIdentification and Analysis of Biomarkers for the Development of Adenocarcinoma from Barrett-EsophagusAims: Optimization of pathogenesis, diagnosis and therapy of esophageal cancer

Identification of predictive biomarkers in Barret syndrome Implementation of PAXgene Tissue for clinical application Validation of tumor evolution theory (dysplasia caused by cardia stem cells) Up to 2000 patients

01.05.2023 46

ReferencesThe STRATFix Project

“Enabling stratified medicine with novel fixatives for improved preanalytical pathology workflows”Formaldehyde free workflows in clinical routineSolid tissue, fine needle biopsies, bloodPartners:

new technology and workflow for integrated collection, stabilization and purification of circulating...

Healthcare