For pathway key and background please visit:

www.cellsignal.com/nfkb

NF-κB Signaling

ub

TRADD

TRAF6

TRAF6

Pellino

β-TrCP

TAK1

JNK

ub

TAB1/2

ub

A20

RNF11

IL-1R

IKKβIKKγ/NEMO

IKKα

RIP

Tax

c-IAP1/2

β-TrCP

GF-Rs

TRAF2/5

TNFR

Ras

Cot

Akt

PI3K

PDK1

ub

TRAF3

LTβR,CD40,BR3

NIK

TRAF2TRAF2/6

SHARPIN

IKKαIKKα

ub

RelBNF-κB2p100ub

IKKαIKKαRelBNF-κB2

p52

RelBNF-κB2p52

H3PARP1

IKKαIKKα

NAP1

PCAF

acIKKγ/NEMO

ATM

SUMO

SUMO

IKKγ/NEMO

NAK

HDACPIASg

CBP/p300

RSK1

PKA C CK2NF-κBp50/52

p65/RelA

NF-κBp50/52

p65/RelA

GSK-3β

MSK1

IKKα/β/θ

PKCζ

RelA/cRel

NF-kB1p50

IκBα/β/θ IκBαNF-κB2

p52

p65/RelA

ub

p38

CK2

NF-κB1p50

RelA/cRel IκBα/θNF-κBp50/52

NF-κBp50/52

Bcl-3

CYLD

ub

ub

ub

ub

ub

ub

ITCH

ELKS

ubc13

IκBζ

Ubc13

HOIPHOIL1

UEV1A

ub

ubIRAK1/4

ProteasomalDegradation

Survival, Proliferation, Inflammation, Immune Regulation Lymphogenesis, B Cell Maturation

ProteasomalProcessing

GenotoxicStress

Stress: ROIs,UV, metals,

ischemia, shear

IL-1

LT, CD40L,BAFF/BLys

Growth Factors:BMP, EGF, HGH,

Insulin, NGF, TGF-a

TNF

UV

For detailed signaling,see BCR Pathway.

Ag-MHC

For detailed signaling,see TCR Pathway.

LPS, CpG, ssRNA, dsRNA

For detailed signaling,see TLR Pathway.

CYLD

LUBAC

K48-ubiquitin

K63-ubiquitin

ub

ub

K48-ubiquitin

K63-ubiquitin

ub

ub

Nucleus

Cytoplasm

ub

c-IAP1/2BCR

TAX1BP1

MyD88

Ag

TCRTLRs

Nuclear-cytoplasmicshuttling of non-

phosphorylated forms

rev. 04/09

Nf-κB Signaling

www.cellsignal.com/cellbiologyFor Research Use Only. Not For Use In Diagnostic Procedures.

www.cellsignal.com/cellbiologyFor pathway key and background please visit:For pathway key and background please visit:

ANTIBODIES & KITS APPLICATIONS REACTIVITY

#5630 A20/TNFAIP3 (D13H3) Rabbit mAb H, M, R, Mk H, M, R, Mk

#4664 ABIN-1 Antibody WB, IP H, M, R, (Mk)

#12629 Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb

WB, IP H, M, (Mk)

#3045 Acetyl-NF-κB p65 (Lys310) Antibody WB, IP H, M, (R, Mk, B, Dg)

#9242 IκBα Antibody WB, IP H, M, R, Mk, B, Dg, Pg

#4812 IκBα (44D4) Rabbit mAb WB, IP H, M, R, Hm, Mk, Mi,

#4814 IκBα (L35A5) Mouse mAb (Amino-terminal Antigen)

WB, IP, IHC-P, IF-IC, F H, M, R, Mk, B, Pg, GP

#7523 IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (PE Conjugate)

F H, M, R, Mk, B, Pg, GP

#2859 Phospho-IκBα (Ser32) (14D4) Rabbit mAb WB, IP H, M, R, Mk, (C, B, Dg, Pg, GP)

#9246 Phospho-IκBα (Ser32/36) (5A5) Mouse mAb

WB H, M, R, Mk, (B, Dg, Pg, GP)

#2682 IKKα Antibody WB, IP H, M, R, Mk, (B)

#11930 IKKα (3G12) Mouse mAb WB, IF-IC, F H, Mk

#2078 Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb

WB H, M, (R, Mk, B)

#7073 PathScan® Phospho-IKKα (Ser176/180) Sandwich ELISA Kit

ELISA H

#2697 Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb

WB, IHC-P, IHC-F, F H, M, R, Mk, (B)

#14938 Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb (PE Conjugate)

F H, M, R, Mk, (B)

#8943 IKKβ (D30C6) Rabbit mAb WB, IP H, M, R, Mk

#2678 IKKβ (L570) Antibody WB, IP H, M, R, Hm, Mk, B, GP

#2684 IKKβ Antibody WB H, Mk

#2695 IKKγ (DA10-12) Mouse mAb WB H, R,

#2685 IKKγ Antibody WB H, M, R, Mk

#3416 IKKε (D61F9) XP® Rabbit mAb WB, IP, IF-IC, F M, R,

#8766 Phospho-IKKε (Ser172) (D1B7) Rabbit mAb WB, IP H, (M, R, Mk, Dg)

#8242 NF-κB p65 (D14E12) XP® Rabbit mAb WB, IP, IHC-P, IF-IC, F, ChIP, ChIP-seq

H, M, R, Hm, Mk, Dg

#49445 NF-κB p65 (D14E12) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)

IF-IC, F H, M, R, Hm, Mk, Dg

#6956 NF-κB p65 (L8F6) Mouse mAb WB, IP, IHC-P, IF-IC, F, ChIP

H, M, R, Hm, Mk, Mi, B, Dg, Pg

#7174 PathScan® Total NF-κB p65 Sandwich ELISA Kit

ELISA H, M

#3039 Phospho-NF-κB p65 (Ser468) Antibody WB, IP H, M, R

#3036 Phospho-NF-κB p65 (Ser536) (7F1) Mouse mAb

WB H, M, R, Mk, Mi, (Dg)

#3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb

WB, IP, IF-IC, F H, M, R, Hm, Mk, Pg, (Dg)

#4887 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor® 647 Conjugate)

F H, M, R, Hm, Mk, Pg, (Dg)

#3031 Phospho-NF-κB p65 (Ser536) Antibody WB H, M, R, Mk, (Dg, Pg)

#7173 PathScan® Phospho-NF-κB p65 (Ser536) Sandwich ELISA Kit

ELISA H, M

#13586 NF-κB1 p105/p50 (D4P4D) Rabbit mAb WB, IP, IF-IC, F, ChIP H, M, R

#3035 NF-κB1 p105/p50 Antibody WB, IP, ChIP H, Mk

#4806 Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb

WB, IP H, M, R, Mk

#3017 NF-κB2 p100/p52 (18D10) Rabbit mAb WB, IHC-P, F H, Mk

ANTIBODIES & KITS APPLICATIONS REACTIVITY

#4882 NF-κB2 p100/p52 Antibody WB, IP H, M, R, Mk

#4810 Phospho-NF-κB2 p100 (Ser866/870) Antibody

WB, IP H, M, (R, B, Dg)

#4994 NIK Antibody WB H, M, (R, Mk, B, Hr)

#67489 c-Rel (D3B8S) Rabbit mAb W, IF-IC H, M

#12659 c-Rel (D5G1A) Rabbit mAb (ChIP Formulated)

ChIP, ChIP-seq H, (R, Mk)

#10544 RelB (D7D7W) Rabbit mAb WB, IP, IHC-P, F, ChIP H, M, R

#5025 Phospho-RelB (Ser552) (D41B9) XP® Rabbit mAb

WB, IP, IF-IC, F H, M, (R, Mk, B, Dg)

#4142 RIP2 (D10B11) Rabbit mAb WB H, M, R, Mk

#14397 Phospho-RIP2 (Ser176) (E1I9J) Rabbit mAb WB, IP H

#3226 TAB1 (C25E9) Rabbit mAb WB, IP H, Mk

#2141 TANK Antibody WB, IP H, M, R, (Mk, B, Dg)

#3504 TBK1/NAK (D1B4) Rabbit mAb WB, IP H, M, R, Mk

#3013 TBK1/NAK Antibody WB, IP H, M, R, Mk

#5483 Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb

WB, IP, IF-IC, F H, M, (R, Mk, X, B, Dg)

#13498 Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb (PE Conjugate)

F H, M, (M, R, Mk, X, B, Dg)

#4729 TRAF3 Antibody WB H, M, R, Mk

#18527 TRAF4 (D1N3A) Rabbit mAb WB H, M

APPLICATIONS & REACTIVITY KEY: WB Western Blotting / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F Flow Cytometry / ChIP Chromatin Immunoprecipitation / -IC Immunocytochemistry / -P Paraffin / -F Frozen / E-P Peptide ELISA / H human / M mouse / R rat / Hm hamster / Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebrafish / B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all species expected / ( ) 100% sequence homology

www.cellsignal.com/nfkb

© 2018 Cell Signaling Technology, Inc. Cell Signaling Technology, CST, and XP are trademarks of Cell Signaling Technology, Inc. U.S. Patent No. 5,675,063. All other trademarks are the property of their respective owners.

Key Products for NF-κB SignalingVisit www.cellsignal.com for full listing of antibodies, related kits, and reagents.

18-IM

M-P

WH-

8361

-E1

For R

esea

rch

Use

Only

. Not

For

Use

In D

iagn

ostic

Pro

cedu

res.

Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033: Confocal IF analysis of HeLa cells, serum starved (left) or TNF-α treated (#8902 at 20 ng/ml for 20 min, right), using #3033 (green). Actin filaments have been labeled with Alexa Fluor® phalloidin 555 (red).

74,610 kb

[0-30]

IL-8

mb 20 mb 40 mb 60 mb 80 mb 100 mb 120 mb 140 mb 160 mb 180 mb[0-20]

NF-κB p65 (D14E12) XP® Rabbit mAb #8242: Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated with hTNF-α #8902 (30 ng/ml, 1 hr) and #8242, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared from 5 ng enriched ChIP DNA using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®, and sequenced on the Illumina NextSeq. The figure shows binding across IL-8, a known target gene of NFκB. For additional ChIP-seq tracks, please download the product data sheet.