non-invasive genetic methods to study wildlife populations ... manseau.pdf · non-invasive genetic...
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Micheline Manseau, PhD
Parks Canada
&
Natural Resources Institute, University of Manitoba
www.lecol-ck.ca
Non-invasive genetic methods to study wildlife
populations – new possibilities
Information required to support wildlife management …
Wildlife conservation
Methods?
• Species distribution
• Population boundaries
• Population size and trend
• Impact of land use activities
• Historical perspectives
DNA extraction & marker amplification, hormones analysis
Ball, Pither, Manseau, Petersen, Clark, Morrill and Wilson. 2007. Conservation Genetic. 8: 577-586
Nuclear DNA (microsatellite)
Mitochondrial DNA
Gender marker
Hormones and isotopes analysis
Cullingham, Curteanu, Ball , Manseau and Wilson. 2010. Journal of Wildlife Management. 74: 849-859
Morden et al. 2011. Journal of Wildlife Management . 75:1426–1435.
Genetic, hormones and isotopes analysis
Species identification and distribution, biodiversity, diet analysis
Molecular Ecology - Special Issue – Environmental DNA
Volume 21, April 2012
Banfield. 1961. National Museum of Canada Bulletin.
Weckworth, Musiani, McDevitt, Hebblewhite and Mariani. 2012. Molecular Ecology. Early View.
Klutsch, Manseau and Wilson. 2012. Proceedings of the Royal Society B (submittted).
Species and subspecies characterization, conservation units
Population genetic structure and characteristics
Polfus, Galpern, Manseau and Wilson. 2012. Joint Congress on Evolutionary Biology.
Population genetic structure and characteristics
Ball, Fennigan, Manseau and Wilson. 2010. Conservation Genetics. 11:2131–2143
Amount and directionality of animal movement between populations
Ball, Fennigan, Manseau and Wilson. 2010. Conservation Genetics. 11:2131–2143.
Frequency-based assignment tests
Thompson, Manseau and Wilson. 2012. Global Change (in prep.)
Current and historical gene flow, historical ecology
Petersen, Manseau and Wilson. 2010. Journal of Mammalogy. 91: 698-711.
Population size, trend, individual fitness, sociogenetic
Kendall et al. USGS Glacier Field Station, Montana
Population size, trend, individual fitness, sociogenetic
Scoring genetic profiles
Galpern, Manseau, Hettinga, Wilson and Smith. 2011. Molecular Ecology Resources. 12: 771–778.
Finding unique genotypes and replicates - Allelematch
Galpern, Manseau, Hettinga, Wilson and Smith. 2011. Molecular Ecology Resources. 12: 771–778.
Population parameters
Individual genotypes - markers • Population sizes
• Population trends
• Survival rate
• Pregnancy rate (hormones)
• Sex ratio
Individual genotypes – alleles • Genetic diversity, inbreeding coefficient
• Effective population sizes
• Individual-based analysis, spatial autocorrelation
in measures of relatedness
• Pedigrees, fine-scale social organization,
sociogenetic
• Mitochondrial DNA, Y-chromosome
Capture Mark Recapture
Closed and Open Population Models
Estimates of survival (Φ), population growth trend (λ) and recruitment
derived from sampling intervals over long timeframe. Can be gender
specific.
Used to estimate population rate of change
Hettinga , Arnason, Manseau, Cross, Whaley and Wilson. 2011. Journal of Wildlife Management. DOI:
10.1002/jwmg.380.
North Interlake herd
Population parameters Sex ratio 60F:40M 2005-2010
Population estimates – CMR models
2007 : 98 (lower), 41 (upper)
2008 : 79 (lower), 32 (upper)
2009 : 73 (lower), 31 (upper)
Survival rates: 75% (F), 65% (M)
Lambda (5 years): 0.83 (0.07) (F), 0.95 (0.06) (M)
Pregnancy rate: 85%
Expected heterozigozity: 0.63 (lower), 0.69 (upper)
Inbreeding coefficient: 0.05 (lower), 0.07 (upper)
Genetic cluster: small geographical area
Population assignment: 100% (lower), 96% (upper)
Gene flow: limited and northward
Historical gene flow: significant and bidirectional
Haplotypes: 9
Haplogroup origin: south of the Laurentian ice sheet
Hettinga , Arnason, Manseau, Cross, Whaley and Wilson. 2011. Journal of Wildlife Management. DOI:
10.1002/jwmg.380.
Ball, Fennigan, Manseau and Wilson. 2010. Conservation Genetics. 11:2131–2143.
If animals are not
moving freely and
mixing their genes…
individuals in the same
habitat cluster should be
more closely related than
individuals in separate
clusters
Galpern, Manseau and Fall. 2011.
Biological Conservation. 144:44-55
Galpern, Manseau and Wilson. 2012.
Molecular Ecology. Early View.
Landscape genetic
A
collaborative
research
framework
that could
involve
citizens.
… new methods to better understand the species’ biology and
ecology at different spatial and temporal scales.
- non-invasive method
- large sample size
- longer time frame, both genders
- a marker and a source of information
Collaborators & Contributors Paul Wilson, Paul Galpern, Corneylia Klutsch, Jean Polfus, Laura Thompson, Peter Hettinga,
Laura Fennigan, Marina Kerr, Karen Smith, Mark Bradley, Leyla Neufeld, Saakje Kazenburg,
Maria Arlt, Casidhe Dyke, Sones Keobouasone, Dan Frandsen, Tim Trottier, Al Arsenault, Fiona
Moreland, Brad Tokaruk, Ed Kewal, Mark Ball, Andrew Fall, Marie-Josée Fortin, Dan O’Brien,
Jeff Clark, Stephen Petersen, Richard Pither, Jennifer Keeney, Glen Brown, Kent Whaley, Dale
Cross, Vicki Trim, Daryll Hedman, Fiona Scurrah
Project funded by CRD-NSERC, Strategic NSERC, Parks Canada (Jasper, Banff, Prince Albert, Grasslands, Wood
Buffalo, Quttinirpaaq,Wapusk National Parks), Endangered Species at Risk Funds, Sask
Environment, Prince Albert Model Forest, Nunavut Government, Weyerhaeuser, Manitoba
Conservation, Manitoba Hydro, Habitat Stewardship Program, NSERC, GEOIDE Centre of
Excellence, Trent University, University of Manitoba