Novocastra HD - HematopathologyHighly Definitive Antibodies

Trust the ‘HD’ difference – Highly Definitive antibodies for diagnostic confidence.• Independently benchmarked by external QA* vs leading equivalents• Simplify antibody selection with Novocastra HD, a new trusted standard in IHC, delivering high quality staining for your diagnostic service.

*Independent analysis commissioned by Leica Biosystems and conducted by NordiQC according to the manufacturers’ instructions for use and on the corresponding staining platform.

Anaplastic Large Cell LymphomaStrong nuclear and cytoplasmic staining in the neoplastic cells.

Human Follicular LymphomaNeoplastic cells show a moderate, predominantly cytoplasmic staining reaction.

Human Diffuse Large B Cell LymphomaThe majority of neoplastic cells show a moderate to strong nuclear staining reaction.

Human ThymomaNeoplastic cells show a moderate to strong and distinct membrane staining reaction.

Human Lymph Node, T Cell LymphomaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells show a strong staining reaction.

T Cell LymphomaNote membrane staining of tumor cells.

T Cell LymphomaTumor cells show a strong membrane staining reaction.

T Cell LymphomaNote intense membrane staining of tumor cells.

BOND 7mL PA0306

Liquid 0.5mL NCL-L-ALK

Anaplastic Lymphoma Kinase (ALK)

Clone 5A4

BOND 7mL PA0117

Liquid 1mL NCL-L-bcl-2

Bcl-2 Oncoprotein Clone Bcl-2/100/D5

BOND 7mL PA0204

Liquid 1mL NCL-L-Bcl-6-564

Bcl-6 Oncoprotein Clone LN22

BOND 7mL PA0235

Liquid 0.5mL NCL-L-CD1a-235

Liquid 1mL NCL-L-CD1a-235

CD1a Clone MTB1

CD3 Clone LN10

BOND 7mL PA0553

BOND 30mL PA0122

Liquid 1mL NCL-L-CD3-565

CD4 Clone 4B12

BOND 7mL PA0427

Liquid 0.5mL NCL-L-CD4-368

Liquid 1mL NCL-L-CD4-368

BOND 7mL PA0168

Liquid 0.5mL NCL-L-CD5-4C7

Liquid 1mL NCL-L-CD5-4C7

CD5 Clone 4C7 CD7 Clone

LP15

BOND 7mL PA0266

Liquid 1mL NCL-L-CD7-580

Novocastra HD - Hematopathology

BOND 7mL PA0843

Liquid 0.5mL NCL-L-CD19-163

Liquid 1mL NCL-L-CD19-163

Human Lymph Node, T Cell LymphomaNeoplastic cells show a moderate to strong, distinct membranous staining reaction.

Human Lymphoblastic LymphomaIntense membrane staining of neoplastic lymphoid cells.

Human Hairy Cell LeukemiaMembrane staining of malignant cells.

Pre B Acute Lymphoblastic LymphomaNeoplastic cells show a moderate to strong predominantly membrane staining reaction.

Mantle Cell LymphomaNote intense membrane staining of malignant lymphocytes.

Follicular B Cell LymphomaFollicular B cell lymphoma showing a strong membrane staining reaction.

Human TonsilNK cells and CD4/CD8 double positive T cells show a weak to moderate and distinct membrane staining reaction while the majority of lymphocytes are unstained.

Mantle Cell LymphomaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells show a strong staining reaction.

Human TonsilGerminal centre macrophages show a strong cytoplasmic staining reaction, while the interfollicular macrophages show correct weak to moderate staining reaction.

Hodgkin’s LymphomaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells show a strong staining reaction.

Human LymphomaNote membrane staining of endothelial cells.

Acute Myeloid LeukemiaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells, as expected, show a strong staining reaction.

CD19 Clone BT51ECD8 Clone 4B11

BOND 7mL PA0183

Liquid 0.5mL NCL-L-CD8-4B11

BOND 7mL PA0270

BOND 30mL PA0131

Liquid 1mL NCL-L-CD10-270

CD10 Clone 56C6

BOND 7mL PA0554

Liquid 1mL NCL-L-CD11c-563

CD11c Clone 5D11

CD20 Clone L26

BOND 7mL PA0200

BOND 30mL PA0359

Liquid 0.5mL NCL-L-CD20-L26

Liquid 1mL NCL-L-CD20-L26

BOND 7mL PA0169

Liquid 0.5mL NCL-L-CD23-1B12

Liquid 1mL NCL-L-CD23-1B12

CD23 Clone 1B12

BOND 7mL PA0790 NEW

Liquid 1mL NCL-L-CD30-591 NEW

CD30 Clone JCM182 CD31 Clone

JC70A

BOND 7mL PA0414 NEW

Liquid 1mL NCL-L-CD31-607 NEW

BOND 7mL PA0555

Liquid 1mL NCL-L-CD33 NEW

CD33 Clone PWS44

BOND 7mL PA0042

Liquid 0.5mL NCL-L-LCA

Liquid 1mL NCL-L-LCA

CD45 Clone X16/99

BOND 7mL PA0191

Liquid 1mL NCL-L-CD56-504

CD56 Clone CD564 CD68 Clone

514H12

BOND 7mL PA0273

Liquid 1mL NCL-L-CD68

Human AppendixIntense staining of plasma cells and secreted immunoglobulin A.

Myeloid SarcomaNote a selective medium to strong staining of neoplastic cells.

Mantle Cell LymphomaMantle cell tumor cells show moderate staining, with a stronger reaction seen in heavy chain expressing plasma cells.

Control Tissue, Human ColonIntense staining of the epithelium in the crypts.

Human TonsilEndothelial cells show a moderate to strong staining reaction.

B Cell Chronic Lymphocytic LeukemiaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells show a strong staining reaction.

High Grade B Cell Immunoblastic LymphomaIntense nuclear staining in proliferating B cells.

PlasmacytomaNote intense membrane staining of tumor cells.

Human Gastrointestinal Stromal TumorNote intense membrane staining of tumor cells.

BOND 7mL PA0007 NEW

Liquid 1mL NCL-L-CD117-032 NEW

CD117 (c-kit Oncoprotein)

Clone EP10

BOND 7mL PA0088 NEW

Liquid 1mL NCL-L-CD138-153 NEW

CD138 Clone MI15

Liquid 1mL NCL-L-CD163

CD163 Clone 10D6

Cytokeratin 20 Clone Ks20.8

BOND 7mL PA0022

BOND 30mL PA0037

Liquid 0.5mL NCL-L-CK20

Liquid 1mL NCL-L-CK20

Factor VIII related antigen (von Willebrand Factor)

Clone 36B11

BOND 7mL PA0400

Liquid 1mL NCL-L-vWF

Immunoglobulin A Clone N1CLA

Liquid 1mL NCL-L-IgA

Immunoglobulin D Clone DRN1C

BOND 7mL PA0061 He Only

Liquid 1mL NCL-L-IgD NEW

Kappa Light Chain Clone CH15 Ki67 Clone

MM1

BOND 7mL PA0606 He Only

Liquid 1mL NCL-L-KAP-581

BOND 7mL PA0118

BOND 30mL PA0410

Liquid 1mL NCL-L-Ki67-MM1

Lambda Light Chain Clone SHL53

BOND 7mL PA0570 He Only

Liquid 1mL NCL-L-LAM-578

B Cell Chronic Lymphocytic LeukemiaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells show a strong staining reaction.

BOND 7mL PA0129

Liquid 1mL NCL-L-MUM1

Multiple Myeloma Oncogene 1 (MUM-1)

Clone EAU32

Hodgkin’s LymphomaA moderate to strong predominantly nuclear staining reaction is seen in the Reed-Sternberg cells and plasma cells.

Invasive Breast CarcinomaIntense staining of invasive tumor cells.

Cytokeratin 7 Clone RN7

BOND 7mL PA0942

BOND 30mL PA0138

Liquid 1mL NCL-L-CK7-560

Novocastra HDHighly Definitive Antibodies

Benefits of Ready-to-Use antibodies for BOND

✓ Optimized for staining performance on BOND

✓ Validated antibody concentrations

✓ Plug and play

✓ Standardized assay

✓ Eliminate variability in antibody preparation

Benefits of concentrated liquid antibodies

✓ Flexibility of use on various platforms1

✓ Adaptable to a variety of detection systems1

✓ Adjustable concentration to suit user’s tissue1

✓ Economical option that retains confidence in clone selection

1. The performance of antibodies should be validated when used with automated platforms or manual staining systems other than stated on Instructions for Use.

Improve workflow efficiency and streamline validation.

95.11988 Rev D · 07/2015 · Copyright©2015 Leica Biosystems Newcastle Ltd. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. Novocastra and BOND are trademarks of Leica Biosystems and its affiliates. Other logos, product and/or company names might be trademarks of their respective owners. The EP clones have been created by Epitomics Inc., using Epitomics’ proprietary rabbit monoclonal antibody technology covered under Patent No.’s 5, 675, 063 and 7, 402, 409

Pre B Cell Acute Lymphoblastic LeukemiaTumor cells show a strong, nuclear staining reaction.

Pre B Cell Acute Lymphoblastic LeukemiaNeoplastic cells show a strong and distinct nuclear staining reaction.

Human Kidney, Wilms’ TumorNuclear staining of invasive tumor cells.

BOND 7mL PA0552

Liquid 1mL NCL-L-PAX-5

Pax-5 Clone 1EW

BOND 7mL PA0339

Liquid 0.5mL NCL-L-TdT-339

Liquid 1mL NCL-L-TdT-339

Terminal Deoxynucleotidyl Transferase (TdT)

Clone SEN28

BOND 7mL PA0562

Liquid 1mL NCL-L-WT1-562

Wilms’ Tumor Clone WT49