nsta web seminar: teach science concepts and inquiry with food:
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LIVE INTERACTIVE LEARNING @ YOUR DESKTOP. NSTA Web Seminar: Teach Science Concepts and Inquiry with Food: The Chemistry of Color Additives. Tuesday, December 2, 2008. The Chemistry of Color Additives. Bhakti Petigara Harp, Ph.D. Chemist, Color Technology Team - PowerPoint PPT PresentationTRANSCRIPT
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NSTA Web Seminar:
Teach Science Concepts and Inquiry with Food:
The Chemistry of Color Additives
LIVE INTERACTIVE LEARNING @ YOUR DESKTOP
Tuesday, December 2, 2008
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The Chemistry of Color Additives
Chemist, Color Technology TeamOffice of Cosmetics and Colors
U.S. Food and Drug Administration
Bhakti Petigara Harp, Ph.D.
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Modules Basic concepts of color History of color additives Definition of color additives
Properties of color additives Certified color additives
Structures Uses Specifications
Certification-exempt color additives Structures Uses
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Module 1
Basic Concepts of Color
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Electromagnetic spectrum
www.owlnet.rice.edu/~psyc351/Images/Electroma
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Basic concepts of color Interaction of visible light with a
compound Wavelengths of light Types of atoms in the compound
Dye molecules contain electrons at many energy levels
Some of the electrons can absorb visible light These electrons undergo transitions from
lower to higher energy levels Absorption removes some visible
light We see the complementary color
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Production of complementary colors
25.198 Peak 20236.9
316.1
493.8
660.7
nm
300.00 400.00 500.00 600.00 700.00
D&C Red No. 6
• 400-430 nm (violet) see yellow• 430-480 nm (blue) see orange• 480-550 nm (green) see red• 550-600 nm (yellow) see violet• 600-700 nm (red) see blue
Absorption bands
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What color would you see?
25.722 Peak 10
306.6 406.8
630.2
719.5 790.5
nm
200.00 300.00 400.00 500.00 600.00 700.00 800.00
• 400-430 nm (violet) see yellow• 430-480 nm (blue) see orange• 480-550 nm (green) see red• 550-600 nm (yellow) see violet• 600-700 nm (red) see blue
Absorption bands
A. RedB. YellowC. Blue
Multiple Choice
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What color would you see?
A. RedB. YellowC. Blue
Multiple Choice
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Absorptivity value Measure of dye’s ability to absorb light High absorptivity value: more efficient, more
economical Dye absorbs a large amount of energy so you only need a
small amount in the product Low absorptivity value: not as efficient, less
economical Dye absorbs less energy so more is needed in the product
400.0 500 600 700 750.0
0.00
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
1.08
NM
A
629.56
408.42
400.0 500 600 700 750.0
0.00
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.00
nm
A
644.94
FD&C Blue No. 1, α = 0.16 D&C Green No. 5, α = 0.02
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Let’s pause for two questions…
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Module 2
History of color additives
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Why do we use food coloring?
Use your chat box to respond.
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Purpose of food coloring Provides identity to foods
Red coloring of maraschino cherries (naturally beige) Flavors of Lifesaver candies
For effect Children's cereals Green ketchup Cake decoration
To mask natural variation in color Citrus Red No. 2 in orange skins
For enhancing naturally occurring colors Astaxanthin in salmon feed
To protect flavors and vitamins from damage or color loss
Light, air, moisture Temperature extremes, other storage conditions
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Early food and cosmetic uses Foods colored with spices and minerals
Paprika, turmeric, saffron, iron oxides Wine artificially colored in 300 B.C.
Cosmetics from vegetable and mineral sources White lead Kohl (contains lead or antimony) Copper ore for eye shadow Vegetable extracts for the skin
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Early hair dyes Used by ancient Egyptians First hair dyes were from plants
Nutgalls from oak trees Pyrogallol, tannic acid Rastik
Lawsonia plant Henna
Metallic dyes Lead comb dipped in vinegar darkened hair Copper salts made hair dark and brittle
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Development of synthetic dyes
Mauveine – first synthetic organic dye 1856, William Henry Perkins Accidental discovery, trying to
synthesize quinine Proved good for dyeing silk and other
textiles Coal-tar dyes
By-products of coal processing Made from petroleum or coal sources
Today hundreds of synthetic dyes
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Let’s pause for two questions…
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Module 3
Definition of color additives
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What is a color additive?
Imparts color to a food, drug, cosmetic, or medical device
Includes black, white, intermediate grays
May form after reacting with skin (e.g., dihydroxyacetone)
May have other functions in product (e.g., TiO2 or ZnO in sunscreens)
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What is a color additive, from a regulatory standpoint?
Straight colors 21 CFR Part 73 – exempt from certification 21 CFR Part 74 – subject to certification
Lakes 21 CFR Part 82 – provisionally listed lakes,
subject to certification FD&C Red No. 40 lakes – listed in Part 74 Carmine – listed in Part 73
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What is a straight color? 21 CFR Part 73-certification exempt
Synthetic organic dyes Inorganic pigments Plant (botanical) derivatives Insect extract
21 CFR Part 74-certified color additives Synthetic organic dyes (also called coal-tar
dyes) Carbon pigments
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What is a lake?
21 CFR Parts 73, 74, and 82 all contain listed lakes
A lake is a water-insoluble pigment composed of a water-soluble straight color an insoluble substratum a cation precipitant
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How many color additives are permitted in the U.S.?
20 64 109
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Color additives permitted for use in various countries
Country No. of color additives permitted
U.S. (straight colors) 64Certified for food 9Certified for drugs 36Certified for cosmetics 35Certified for medical devices 7Certification-exempt for food 32Certification-exempt for drugs 28Certification-exempt for cosmetics 29Certification-exempt for medical devices 21
E.U. (straight colors and lakes) 154Japan (straight colors and lakes) 83
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Let’s pause for two questions…
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Module 4
Certified color additives Structures Uses Specifications
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Properties of coal-tar dyes Conjugation from many double bonds
N=N (azo group) C=O (carbonyl group) C6H6 (phenyl group)
Functional groups added for water solubility SO3
- (sulfonate group) COO- (carboxylate group)
Water-soluble dyes Na+ salts
Insoluble pigments and lakes Ca+2 or Ba+2 salts Alumina or rosin substrata
“Oil-soluble” dyes Soluble in organic solvents
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Types of certified coal-tar dyes Triphenylmethane Indigoid Fluoran Xanthene Anthraquinone Pyrene Monoazo Disazo Quinoline Carbon pigments
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What color do you think is in blue Gatorade?
Multiple Choice
A. FD&C Blue No. 1B. FD&C Green No. 3C. D&C Blue No. 4
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What color do you think is in blue Gatorade?
Multiple ChoiceA. FD&C Blue No. 1B. FD&C Green No. 3C. D&C Blue No. 4
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Color Additive R1 R2
FD&C Blue No. 1 SO3Na H
FD&C Green No. 3 SO3Na OH
D&C Blue No. 4 SO3NH4 H
TRIPHENYLMETHANE DYES
C+
N
C2H5
N
C2H5
SO3-
R2
R1
R1
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Color Additive
FD&C Blue No. 2
D&C Blue No. 6
D&C Red No. 30
INDIGOID DYES
SCl
CH3 O
S Cl
CH3O
N
N
O
O
S
S
NaOO
O
ONa
O
OH
H
N
N
O
OH
H
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Color Additive R1 R2 R3
D&C Orange No. 5 H Br H
D&C Orange No. 10 H I H
D&C Red No. 21 Br Br H
D&C Red No. 27 Br Br Cl
D&C Yellow No. 7H H H
FLUORAN DYESOHO OH
R1
R2 R2
R1
R3
R3
R3
R3
O
O
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Color Additive R1 R2 R3
D&C Orange No. 11 H I H
D&C Red No. 22 Br Br H
D&C Red No. 28 Br Br Cl
D&C Yellow No. 8 H H H
FD&C Red No. 3 I I H
XANTHENE DYESO ONaO
R1
R2R2
R1
R3
R3
R3
R3
CO2Na
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Color Additive R1 R2_______
D&C Green No. 5
Ext D&C Violet No. 2 OH
D&C Green No. 6
D&C Violet No. 2 OH
ANTHRAQUINONE DYES
O
O R1
R2
HN
CH3NaO3S HN
CH3NaO3S
HN
CH3HN
CH3
HN
CH3
HN
CH3NaO3S
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Color Additive
D&C Green No. 8
PYRENE DYE
SO3NaNaO3S
SO3NaHO
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Which color additive is a suspected allergen?
FD&C Red No. 40
FD&C Blue No. 1 FD&C Yellow No. 5
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R1NNR2
Color Additive R1 R2
FD&C Red No. 40
FD&C Yellow No. 5
FD&C Yellow No. 6
D&C Orange No. 4 NaO3S
HO
MONOAZO DYES
NaO3S
H3C
O
H3C
HO
SO3Na
NaO3S
N
N
NaO2C
SO3Na
HO
NaO3S
HO
SO3Na
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R1NNR2
Color Additive R1 R2
D&C Red No. 6
D&C Red No. 7
D&C Red No. 34
MONOAZO DYES
H3C
SO3NaHO CO2Na
H3C
SO3Ca1/2
HO CO2Ca1/2
SO3Ca1/2
HO CO2Ca1/2
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R1NNR2
Color Additive R1 R2____________
D&C Red No. 36
D&C Red No. 31
MONOAZO DYES
Cl
N+
–O
O
HO
HO CO2Ca1/2
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Color Additive R1 R2 R3
D&C Brown No. 1
D&C Red No. 17
DISAZO DYESR1
NNR2
NNR3
NaO3S
OH
OHCH3
CH3
HO
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Color Additive R
D&C Yellow No. 10 SO3Na (mixture of 6'- and 8'- isomers)
D&C Yellow No. 11 H
QUINOLINE DYES
N
O
OR
6'
8'
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Color Additive Identity
D&C Black No. 2 high-purity carbon black
D&C Black No. 3 high-purity bone char
CARBON PIGMENTS
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Manufacture of D&C Red No. 6
CH3
R1
N+
OH
R2
CH3
N
R1
N
OH
R2
3a, R2 = CO2H 3-Hydroxy-2-naphthoic acid3b, R2 = H 2-Naphthol
2a, R1 = SO3-
Diazotized PTMS2b, R1 = H Diazotized p-toluidine
4a, R1 = SO3-, R2 = CO2
-, Na2 salt D&C Red No. 64b, R1 = SO3
-, R2 = CO2-, Ca salt
D&C Red No. 74c, R1 = H, R2 = CO2
-, Na or Ca1/2 salt Unsulfonated subsidiary color4d, R1 = R2 = H 4-Methyl-Sudan I (Uncarboxylated- unsulfonated subsidiary color)
CH3
R1
NH2
H+, NO2-
1a, R1 = SO3H
PTMS1b, R1 = H p-Toluidine
N
Na+, Ca+2
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Specifications for D&C Red No. 6
(21 CFR 74.1306) Sum of volatile matter, < 10% Ether-soluble matter, pass test (Appendix A) 2-Amino-5-methylbenzenesulfonic acid, sodium
salt, <0.2% 3-Hydroxy-2-naphthalenecarboxylic acid, sodium
salt, <0.4% 3-Hydroxy-4-[4-(methylphenyl)azo]-2-
naphthalenecarboxylic acid, sodium salt, 0.5% p-Toluidine, <15 ppm Lead, < 20 ppm Arsenic, < 3 ppm Mercury, < 1 ppm Total color, > 90%
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Let’s pause for two questions…
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Module 5
Certification-exempt color additives Structures Uses
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Certification-exempt color additives (21 CFR Part 73) Not required to be certified by FDA Manufacturers are responsible for compliance with
CFR specifications Can be inorganic or organic compounds Plant, animal, and mineral sources Have less coloring power Some are less stable and more variable in shade Can vary in composition from batch to batch Can introduce undesirable flavors and odors in
products Can be contaminated with undesirable trace
metals and bacteria
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What are some examples of certification-exempt color additives?
Use your chat box to respond.
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ANNATTO
CO2CH3
CO2H
CH3CH3
CH3
H3C
CO2H
CO2H
CH3CH3
CH3
H3C
+BIXIN
NORBIXIN
Annatto tree (Bixa orellana) produces seed with colored coating Seeds extracted with approved solvents Imparts butter yellow to peach color – used in butter, cheese,
and ice cream
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-CAROTENE
CH3CH3
CH3CH3
CH3
H3C
H3C
CH3
CH3
CH3
Isomer of carotene Synthetically produced from acetone as all trans form Has nutritional value – converted by humans to
Vitamin A Imparts yellow to orange shades in butter, cheese,
juice, baked goods
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COCHINEAL EXTRACT AND CARMINE
Cochineal extract Solution obtained after removing alcohol from extract of
dried bodies of a female insect (Coccus cactic L.) Carminic acid – hydroxyanthraquinone linked to a glucose
unit Typically acidic, orange to red shade
Carmine Calcium or calcium-aluminum lake formed by
precipitating carminic acid onto an aluminum hydroxide substrate
Pigment that imparts red to pink shades in food Both have microbial specification for Salmonella
O
O
HO
OH
OH
OH
HO
O O
OH
OH
OH
OH
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HENNA
Dried leaf of lawsonia plant Imparts a brown, orange-brown, or reddish-brown tint Used for hair coloring Not permitted for use on skin
O
O
OH
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Spices
Saffron
Turmeric
Paprika
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Do you think chicken is artificially colored? How about salmon?
Raise your hand if you think they are.
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Certification-exempt color additives for food use
Tagetes (Aztec marigold) meal and extract – for chicken feed
Astaxanthin – for fish feed Dehydrated beets (beet powder) Ferrous gluconate – for olives Riboflavin Tomato lycopene
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Inorganic color additives Iron oxides
FeO – green Fe2O3 – reddish brown FeO(OH)·H2O – yellow or brown Fe3O4 – black
White color additives Mica – K2Al4(Al2Si6O20)(OH)4
Talc – Mg3Si4O10(OH)2
Titanium dioxide – TiO2
Zinc oxide – ZnO Ultramarines (blue, green, violet) – Nav(AlwSixOy)Sz
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Certification-exempt color additives for drug and cosmetic use
Bismuth citrate (for coloring hair) Dihydroxyacetone (for skin tanning) Guanine (comes from fish scales) Lead acetate (for coloring hair) Pyrogallol (for sutures) Luminescent zinc sulfide (for holiday
make-up)
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Questions?
www.fda.gov search color additives
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Thanks to our presenter, Dr. Bhakti Petigara Harp,
and to the FDA for sponsoring this program
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http://www.elluminate.com
Elluminate logo
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http://learningcenter.nsta.org
NLC screenshot
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• NSDL: Chemistry Comes Alive III: Water
December 9, 2008
• Sally Ride Science: Igniting Students’ Interests in Science Careers
December 10, 2008
• NSTA: Force and Motion: Stop Faking It!
December 11, 2008
http://learningcenter.nsta.org
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National Science Teachers AssociationDr. Francis Q. Eberle, Executive Director
Zipporah Miller, Associate Executive Director Conferences and Programs
Al Byers, Assistant Executive Director e-Learning
LIVE INTERACTIVE LEARNING @ YOUR DESKTOP
NSTA Web SeminarsFlavio Mendez, Senior Director
Jeff Layman, Technical Coordinator