Nucleic Acid PurificationNucleic Acid Purification

Stratagene’s Products forStratagene’s Products forNucleic Acid Purification Nucleic Acid Purification

D N A E xtraction:D N A E xtraction K it

D N A M icroExtraction K itR ecoverE ase D N A Iso lation K it

M in ip rep Purif ication:S trataP rep P lasm id M in ip rep K itS trataP rep P lasm id M id ip rep K it

C learC ut M in ip rep K it

G el Iso la tion:S trataP rep G el Extraction K it

Q uik-P ik E lectroe lution C olum n

PC R P roduct P urif ication:S trataP rep PC R P urif ication K itQ uik-P ik E lectroe lution C ap sule

D N A P urif ication

Tota l R N A Purif ication:R N A Iso lation K it

M icro R N A Iso lation K it

M essenger R N A Iso lation:M essenger R N A Iso lation K it

Poly(A ) Q uik m R N A Iso lation K it

R N A P urif ica iton

Probe P urif ica iton:N ucTrap P robe P urif ication C loum ns

Push C olum n B eta Shie ld D ev ice

Lam bda P urif ication:Lam bda D N A Purif ication K it

D eg radation of N ucle ic A c ids:R N ace-It R ibonuclease C ockta il

P rote in R em oval:S tratac lean R esin

P rote inase K

R elated P roducts

N ucle ic Ac id Purif ica itonProducts

Strataprep Miniprep KitsStrataprep Miniprep Kits Kits for isolation of DNA from plasmid Kits for isolation of DNA from plasmid

minipreps, PCR amplifications and agarose gels minipreps, PCR amplifications and agarose gels utilize a special silica-based fiber matrx to utilize a special silica-based fiber matrx to capture DNA in the presence of a chaotropic capture DNA in the presence of a chaotropic salt (guandinium)salt (guandinium)

High yields of extremely pure DNAHigh yields of extremely pure DNA Fast, easy protocol- simply capture, wash and Fast, easy protocol- simply capture, wash and

elute DNAelute DNA Convenient microspin format compatible with Convenient microspin format compatible with

virtually all microfuges virtually all microfuges No phenol, resin slurries or alcohol precipitationNo phenol, resin slurries or alcohol precipitation DNA elutes in low ionic strength buffer or water DNA elutes in low ionic strength buffer or water

and is application readyand is application ready

Key Selling PointsKey Selling Points

Plasmid Miniprep KitPlasmid Miniprep Kit– Works on all sizes of DNA 2.9 kb plasmids to 45 kb cosmidsWorks on all sizes of DNA 2.9 kb plasmids to 45 kb cosmids– Up to 20 ug DNA from 1.5-3 ml of cultureUp to 20 ug DNA from 1.5-3 ml of culture– DNA transforms E. coli as easily as cesium purified DNADNA transforms E. coli as easily as cesium purified DNA

– Takes as little as 2.5 minutes/prep for 30 cultures at onceTakes as little as 2.5 minutes/prep for 30 cultures at once PCR Purification KitPCR Purification Kit

– Up to 10 ug of PCR Product rapidly purifiedUp to 10 ug of PCR Product rapidly purified– Nearly quantitative removal of PCR primers and efficient removal of nonspecific amplification Nearly quantitative removal of PCR primers and efficient removal of nonspecific amplification

products smaller than 100 bpproducts smaller than 100 bp– Easy procedure does not require removal of oil overlayEasy procedure does not require removal of oil overlay

DNA Gel Extraction KitDNA Gel Extraction Kit – Rapidly purify up to 10 ug DNA/prepRapidly purify up to 10 ug DNA/prep– conventional and low melt agaroses and all neutral gel buffersconventional and low melt agaroses and all neutral gel buffers– 75-80% recovery of fragments 100 bp to 23 kb75-80% recovery of fragments 100 bp to 23 kb

Market PotentialMarket Potential

The market potential worldwide for The market potential worldwide for small-scale DNA purification is small-scale DNA purification is estimated at over $100 millionestimated at over $100 million– 30% of the market is comprised of 30% of the market is comprised of

commercial kits and reagents commercial kits and reagents – 70% of the market relies on traditional 70% of the market relies on traditional

methods and procedures (phenol extraction methods and procedures (phenol extraction and ethanol precipitation)and ethanol precipitation)

Who is the Competition? Who is the Competition?

Qiagen and Promega have about 30% market Qiagen and Promega have about 30% market share in the small scale purification market share in the small scale purification market (1997) (1997) – Qiagen ca. $13 million Qiagen ca. $13 million – Promega ca. $8 millionPromega ca. $8 million

Clonetech has recently entered the market Clonetech has recently entered the market promoting NucleoBond, NucleoSpin and promoting NucleoBond, NucleoSpin and NucleoTrap nucleic acid purification productsNucleoTrap nucleic acid purification products

Competing claims about “miniprep speed”Competing claims about “miniprep speed”

Competitive Comparison Competitive Comparison StrataPrep Plasmid Miniprep Kit was tested side-by-StrataPrep Plasmid Miniprep Kit was tested side-by-

side with Qiagen and Promega and found to be as side with Qiagen and Promega and found to be as fast, and in some cases faster! fast, and in some cases faster!

StrataPrep purified PCR products yield nearly TWICE StrataPrep purified PCR products yield nearly TWICE the transformation efficiency as inserts prepared the transformation efficiency as inserts prepared with Qiagens silica-gel membrane methodwith Qiagens silica-gel membrane method

Side-by-side testing with Qiagens kit shows that Side-by-side testing with Qiagens kit shows that StrataPrep PCR purification procedure is superior at StrataPrep PCR purification procedure is superior at removing contaminantsremoving contaminants

Compared to Qiagens Qiaex II method, DNA purified Compared to Qiagens Qiaex II method, DNA purified using StrataPrep DNA Gel Extraction Kit has higher using StrataPrep DNA Gel Extraction Kit has higher cloning efficienciescloning efficiencies

Product PositioningProduct Positioning

StrataPrep Kits address our customers evolving StrataPrep Kits address our customers evolving DNA purification needsDNA purification needs

High yields of DNA can be used immediately in High yields of DNA can be used immediately in todays demanding molecular studiestodays demanding molecular studies – CloningCloning– MutagenesisMutagenesis– SequencingSequencing– Probe GenerationProbe Generation

Convert customers by superior performance Convert customers by superior performance (yield, purity, speed) and value (economically (yield, purity, speed) and value (economically priced and volume pricing for 250 count kits)priced and volume pricing for 250 count kits)

StrataPrep EF Plasmid Midiprep StrataPrep EF Plasmid Midiprep KitKit

More than just a MidiprepMore than just a Midiprep Yields up to 350 ug plasmid DNAYields up to 350 ug plasmid DNA Fast and easy protocolFast and easy protocol Yields endotoxin-free DNA (optional step)Yields endotoxin-free DNA (optional step) No additional ethanol precipitations No additional ethanol precipitations

needed needed DNA is ready for ALL applications DNA is ready for ALL applications

including automated sequencing and including automated sequencing and mammalian transfectionsmammalian transfections

StrataPrep EF Plasmid Midiprep StrataPrep EF Plasmid Midiprep KitKit

Utilizes the same silica-based fiber matrix Utilizes the same silica-based fiber matrix technology as other StrataPrep kitstechnology as other StrataPrep kits

Processing time is 90 minutes or less Processing time is 90 minutes or less (includes endotoxin-free step) vs. 3+ (includes endotoxin-free step) vs. 3+ hourshours

Unique color indication protocol to aid in Unique color indication protocol to aid in successful purificationsuccessful purification

Few kits available with endotoxin-free Few kits available with endotoxin-free puritypurity

StrataPrep EF Plasmid Midiprep StrataPrep EF Plasmid Midiprep kitkit

CompetitionCompetition Qiagen Qiagen

gravity drip columns gravity drip columns purchase endotoxin removal buffer separatelypurchase endotoxin removal buffer separately

PromegaPromeganot transfection grade not transfection grade drip columnsdrip columns

ClontechClontechnot really transfection grade not really transfection grade

Who Are Our Customers?Who Are Our Customers?

Researchers utilizing small scale Researchers utilizing small scale DNA purification technologies can DNA purification technologies can be found across the life science be found across the life science spectrumspectrum– Academic, Government and Private Academic, Government and Private

Research LabsResearch Labs– Diagnostic Testing LabsDiagnostic Testing Labs– BiotechBiotech– PharmaceuticalPharmaceutical

Purification Product Sales Purification Product Sales ToolsTools

CatalogCatalog Product ProfilesProduct Profiles Strategies ArticlesStrategies Articles

The StrataPrep family The StrataPrep family grows...grows...

Not only do we have DNA Not only do we have DNA purification...purification...

StrataPrep Total RNA Miniprep StrataPrep Total RNA Miniprep KitKit

High yields of quality Total RNAHigh yields of quality Total RNA Fast and easy protocolFast and easy protocol Purifies total RNA from cells Purifies total RNA from cells andand tissue tissue Microspin cup configuration Microspin cup configuration

(same StrataPrep microspin cup chemistry)(same StrataPrep microspin cup chemistry) DNase digestion performed on column DNase digestion performed on column

(no phenol extraction or ethanol ppt)(no phenol extraction or ethanol ppt) RNA ready for all applications (Northerns, RNA ready for all applications (Northerns,

RT-PCR, RNase protection assays, etc.)RT-PCR, RNase protection assays, etc.)

StrataPrep Total RNA Miniprep StrataPrep Total RNA Miniprep KitKit

CompetitionCompetition QiagenQiagen

not DNA free, but good yields of RNAnot DNA free, but good yields of RNA

PromegaPromegasimilar spincup config. but varying results (odd similar spincup config. but varying results (odd protocol)protocol)

(also recommend as a DNA purification(also recommend as a DNA purification kit)kit)

Boehringer MannheimBoehringer MannheimDNA free but only recommended for cells, not DNA free but only recommended for cells, not tissuetissue

StrataPrep Total RNA Miniprep StrataPrep Total RNA Miniprep KitKit

Features and BenefitsFeatures and Benefits High yields of quality Total RNAHigh yields of quality Total RNA Fast and easy protocolFast and easy protocol Purifies total RNA from cells Purifies total RNA from cells andand tissue tissue Microspin cup configuration Microspin cup configuration

DNase digestion performed on column DNase digestion performed on column RNA ready for all applications (Northerns, RNA ready for all applications (Northerns,

RT-PCR, RNase protection assays, etc.)RT-PCR, RNase protection assays, etc.)

Genomic DNAGenomic DNADNA Extraction DNA Extraction

Gentle digestion of cellular Gentle digestion of cellular proteins followed by removal of proteins followed by removal of proteins by “salting-out”proteins by “salting-out”

100 to 350 kb range from blood100 to 350 kb range from blood 100 kb from tissue 100 kb from tissue No OrganicsNo Organics

Genomic DNA Genomic DNA DNA MicroExtraction KitDNA MicroExtraction Kit

Samples as small as 1 ul of blood Quick (1 hour) No Organics Very reliable Strategies article Quick Isolation of DNA Suitable for

PCR Using Minute Amounts of Whole Blood vol. 5 pg. 68

StrataClean ResinStrataClean Resin

Patented hydroxylated silica particles. Patented hydroxylated silica particles. Offers a rapid, nontoxic alternative to Offers a rapid, nontoxic alternative to phenol/chloroform for small volume phenol/chloroform for small volume extractions. extractions.

Extracts unwanted enzymes from DNAExtracts unwanted enzymes from DNARE, PCR, and sequencing template prepRE, PCR, and sequencing template prep

Concentrates dilute proteins for micro-Concentrates dilute proteins for micro-analysisanalysis

ClearCut™ Miniprep KitClearCut™ Miniprep Kit

Simple 20-minute plasmid prep method Simple 20-minute plasmid prep method (modif. alkaline lysis, silica resin)(modif. alkaline lysis, silica resin)

No phenol/chlororform or cesium No phenol/chlororform or cesium bandingbanding

Produces pure plasmid for:Produces pure plasmid for:RE, ligation, seq, transformationsRE, ligation, seq, transformations

Buffer exchanges and PCR cleanup in 5 Buffer exchanges and PCR cleanup in 5 minutesminutes

RecoverEase DNA RecoverEase DNA Isolation Isolation

High-molecular weight genomic DNA High-molecular weight genomic DNA from whole tissuefrom whole tissue

No organic extractions or ethanol No organic extractions or ethanol precipitationsprecipitations

DNA is ready to use the next day DNA is ready to use the next day Ideal for use with the Big Blue system Ideal for use with the Big Blue system

and transgenic mutation assaysand transgenic mutation assays

Stratagene has the Stratagene has the Purification Solution! Purification Solution!

Stratagene has a method for all your Stratagene has a method for all your customers nucleic acid purification customers nucleic acid purification needsneeds

The new StrataPrep Kits quickly purify The new StrataPrep Kits quickly purify high quality DNA from minipreps, PCR high quality DNA from minipreps, PCR amplifications and agarose gelsamplifications and agarose gels

When only small amounts of material When only small amounts of material are available use the Micro DNA and are available use the Micro DNA and RNA Kit formatsRNA Kit formats