ocean ecosystem transformation caused by rapid …
TRANSCRIPT
The Pennsylvania State University
The Graduate School
OCEAN ECOSYSTEM TRANSFORMATION CAUSED BY RAPID WARMING AND
SEA LEVEL RISE IN THE PLEISTOCENE CARIACO BASIN (MIS 9-7)
A Thesis in
Geosciences
by
Adriana I. Rizzo
Submitted in Partial Fulfillment
of the Requirements for the Degree of
Master of Science
August 2019
ii
The thesis of Adriana I. Rizzo was reviewed and approved* by the following:
Timothy J. Bralower Professor of Geosciences Thesis Co-Advisor
Katherine H. Freeman Evan Pugh University Professor Departments of Geosciences and Chemistry Thesis Co-Advisor
Mark E. Patzkowsky Professor of Geosciences Associate Head for Graduate Programs and Research, Department of
Geosciences
*Signatures are on file in the Graduate School
iii
ABSTRACT
Local phytoplankton community structure has implications for larger ocean ecosystems and the
global carbon cycle. Understanding the response of these ecosystems to warming in the tropics is
important for understanding future global change. We examined changes in phytoplankton
community composition over a full glacial cycle in MIS 9-7 (330-230 kya) in the sediments from
the Cariaco Basin (ODP Site 1002). Phytoplankton communities were reconstructed using both
calcareous nannoplankton assemblages and sterol and alkenone biomarkers. These data were
integrated with alkenone, microfossil, and sterol proxies for temperature and circulation.
Emiliania huxleyi occurred definitively in the Cariaco Basin for the first time between 250 and
240 kya, possibly due to increased connectivity caused by rising sea level. At the same time,
environmental variability increased during a period of rapid warming from 250-240 kyr,
inducing ecological turnover at both species- and larger clade levels. In general, during
interglacials, the basin was more productive and more stratified, suggesting higher nutrient
fluxes from land and greater rainfall associated with a more northerly ITCZ. This suggests that
current warming may cause similar species introductions, productivity changes, and disruptions
to phytoplankton populations.
iv
TABLE OF CONTENTS
LIST OF FIGURES...................................................................................................................... v
LIST OF TABLES........................................................................................................................ vi
ACKNOWLEDGEMENTS.......................................................................................................... vii
Introduction................................................................................................................................... 1
Pleistocene Climate Changes in the Cariaco Basin........................................................... 3
Methods......................................................................................................................................... 7
Taxonomy.......................................................................................................................... 7
Stratigraphy and age model............................................................................................... 8
Organic geochemistry methods......................................................................................... 11
Statistical analyses............................................................................................................ 13
Results........................................................................................................................................... 15
Discussion..................................................................................................................................... 22
Ecological response of taxa to environmental change...................................................... 22
Glacial/Interglacial trends in productivity and nutrient sourcing..................................... 24
Biomarkers and Nannofossils: a new approach for paleoceanographic reconstruction... 27
Sea level change, biogeography, and dispersal of E. huxleyi......................................... 29
Implications for future change.......................................................................................... 31
Conclusions................................................................................................................................... 33
References..................................................................................................................................... 34
Appendix A: Rarefaction analysis of nannofossils ...................................................................... 41
Appendix B: Nannofossil preservation......................................................................................... 44
Appendix C: Multivariate Analysis.............................................................................................. 46
Appendix D: Biomarker data........................................................................................................ 50
Appendix E: Data plotted against depth....................................................................................... 54
v
LIST OF FIGURES
Figure 1: Seasonal changes in the ITCZ position over South America......................................... 4
Figure 2: Map showing location and modern bathymetry of the Cariaco Basin............................ 6
Figure 3: Sedimentation rate and stratigraphy for studied interval of Hole 1002D...................... 10
Figure 4: Climate trends globally and at Site 1002 ...................................................................... 15
Plate 1: SEM micrograph of morphologies that would be identified as E. huxleyi/R. parvula in
the light microscope...................................................................................................................... 16
Figure 5: Relative abundances of calcareous nannofossils........................................................... 17
Figure 6: Rolling variance for major coccolith species, excluding the Emiliania complex......... 19
Figure 7: Percent organic carbon and biomarker Mass Accumulation Rates............................... 21
Figure 8: Schematic showing ecological response of major phytoplankton clades...................... 22
Figure 9: Schematic summarizing interpretation of glacial-interglacial environmental changes. 26
Figure A1: Rarefaction and species accumulation curves for calcareous nannofossils................ 42
Figure A2: Nannofossil rank-abundance curves........................................................................... 43
Figure B1: Coccolith preservation over time................................................................................ 45
Figure C1: NMDS of nannofossil data set, with fitted environmental variables.......................... 47
Figure C2: DCA of nannofossil data set, with fitted environmental variables............................. 48
Figure E1: Relative abundances of calcareous nannofossil and Pielou's evenness metric vs.
depth.............................................................................................................................................. 54
Figure E2: Temperature and biomarker Mass Accumulation Rates plotted against depth........... 55
vi
LIST OF TABLES
Table 1: First differences correlations (ρ) between phytoplankton biomarkers, paleoceanographic
proxies, and nannoplankton relative abundances......................................................................... 20
Table 2: First differences correlations (ρ) between phytoplankton biomarkers........................... 20
Table D1: Concentrations for measured biomarkers.................................................................... 51
Table D2: Parameters used in mass accumulation rate calculations............................................. 52
Table D3: Mass accumulation rates for measured biomarkers..................................................... 53
vii
ACKNOWLEDGEMENTS
I would like to thank my advisors, Kate Freeman and Tim Bralower, for their feedback
and assistance as well as their willingness to support a project slightly out of their usual realm of
research. Thanks as well to Mark Patzkowsky, my third committee member, for his helpful
feedback and for facilitating an excellent environment for scientific conversations with my peers.
This research was supported by several Penn State Geosciences department scholarships:
the Tait Scholarship in Microbial Biogeochemistry, the Shell Research Facilitation Award, and
several Krynine Travel Grants. Thank you to the donors to the department for your generosity.
Additional funding was provided by the National Science Foundation, award # 1416663.
I would also like to thank Yongsong Huang of Brown University for access GC-FID
instruments in his lab, and to Richard Vachula and Xiangming Zhao, also of Brown University,
for assistance running my samples.
Many thanks to the Freeman lab group, particularly Troy Ferland and Allison Karp, for
their thoughtful feedback and assistance with unruly instruments. Thanks also to Sara Lincoln,
Denny Walizer and Margaret Davis for assisting with various instrument and facilities issues.
Finally, thank you to my family and friends for supporting me through this difficult
journey. In particular, thank you Ashwin for your many sacrifices and the support I've had
through my troubles. I couldn't have done it without you.
1
Introduction
Marine phytoplankton ecosystem structures are important components of the Earth
system and are sensitive to climate and ocean circulation. Marine phytoplankton are key
components of the global carbon cycle and the basis for marine food webs. Coastal and
upwelling zones have the highest rates of carbon fixation per square meter (Knauer, 1993). Due
to their productive fisheries and proximity to human settlements, these regions are of particular
importance for human society but also more subject to human influence (Shackeroff et al., 2009).
Three main clades of phytoplankton, dinoflagellates, diatoms, and coccolithophores dominated
these environments throughout the Cenozoic. Each of these groups has distinct preferences for
nutrients and water column stability, an ecological framework referred to as Margalef's Mandala
(Margalef, 1978).
Changes in phytoplankton species distributions and in group rank abundances can affect
the movement of carbon through the ocean and disrupt food webs at higher trophic levels.
Diatoms and coccolithophores enhance carbon flux to the deep sea by ballasting organic matter
with mineral tests (Honjo, 1976), but the rates of this flux are size- and species-dependent (Ziveri
et al. 2007). In particular, coccolithophores are more efficient at ballasting (Thunell et al., 2007)
and provide a significant flux of inorganic carbon to the deep sea via calcification (Ridgewell,
2005). Harmful Algal Blooms (HAB), monospecific assemblages of toxin-producing diatoms
and dinoflagellates, can kill fish and marine mammals, posing economic threats (Hoagland and
Scatasta, 2006) and additional burdens for the conservation of threatened and endangered species
(Fire and Van Dolah, 2012).
Our rapidly warming modern climate is likely to substantially change the geographic
distribution of many phytoplankton, including HAB species (Hallegraeff, 2010; Kordas et al.,
2
2011). However, disentangling the influence of climate and anthropogenic nutrient inputs
presents a challenge. Additionally, it is not clear how coccolithophores, which are mostly
insensitive to temperature (Hagino et al., 2000) and cosmopolitan in their distribution (Okada
and Honjo, 1973), will respond to climate change. In this study, we seek to understand
oceanographic and climate mechanisms driving the distribution of greater phytoplankton groups
and species distribution of coccolithophores during a warming climate.
These predictive challenges can be mitigated by studying the middle Pleistocene record
of climate-ecosystem interaction, which contains all major species of modern coccolithophores.
The glacial cycle from marine isotope stage 9 to stage 7 is of particular interest for biogeographic
studies because it contains the global first occurrence of Emiliania huxleyi (Raffi et al., 2006).
During this time, global temperatures increased by 5°C (Hönisch et al., 2009) and global sea
level rose by 100 m (Dutton et al., 2009; Lea et al., 2002) within 10 kyr. Although the rate of
change is orders of magnitude below the pace of anthropogenic climate change, ecological
changes in high resolution during the relatively swift warming at the onset of the MIS 7
interglacial can provide meaningful context for understanding the future.
The Cariaco Basin, southern Caribbean Sea, has hydrography sensitive to major changes
in climate and ocean circulation. Moreover, high sedimentation rates enable high temporal
resolution sampling on sub-millennial intervals. Geographically, the region experiences a climate
regime that is sensitive to changes in major patterns of ocean and atmospheric circulation. These
changes have been well characterized by previous research on sediments from Ocean Drilling
Program (ODP) Leg 165 at Site 1002, the source of the samples analyzed in this study. We
hypothesize that changes in water column structure and nutrient delivery associated with
3
circulation and sea level changes provided the primary control on phytoplankton ecosystem
structure.
Pleistocene Climate Changes in the Cariaco Basin
The Cariaco Basin is a pull-apart basin located at 10°N on the Caribbean margin of South
America (Figures 1, 2). Today, the Cariaco Basin is noted for its permanent anoxia below 300 m
(Richards and Vaccaro, 1956). During the Northern Hemisphere winter months, when insolation
is at a minimum at 10° north, the Intertropical Convergence Zone (ITCZ) shifts south (Figure
1A), creating strong easterly trade winds that cause advection-driven upwelling on shallow areas
of the Tortuga Bank, the sill on the northern part of the basin (Astor et al., 2003). During the
summer months (Figure 1B), when insolation is at a maximum at 10°N, the ITCZ moves north,
initiating the rainy season nearby in northern South America and weakening the trade winds
(Astor et al., 2003; Muller-Karger et al., 2001). This shuts down upwelling and reduces
thermocline mixing in the basin; increased fresh water inputs cause a transient decrease in
salinity, which is above 36 ppt other times of the year (Astor et al., 2003; Richards and Vaccaro,
1956). Volumetrically, the fresh water flux from small rivers that empty into the Cariaco Basin is
small compared to that of the Orinoco River, which does not directly flow into the basin but is a
major source of terrestrial inputs to the Eastern Caribbean in general (Richards, 1975).
4
Paleoceanographic studies indicate that climate and oceanographic conditions during
previous interglacial periods broadly resembled the present. Multiple proxies indicate anoxia set
in around 14.5 kya in association with increased productivity after the Last Glacial Maximum
(LGM) (Peterson et al., 2000b, 1991; Yarincik et al., 2000a). In contrast, during glacial and
cooler periods, the basin was generally less productive (Herbert and Schuffert, 2000; Peterson et
Figure 1: Seasonal changes in the ITCZ position over South America. The Cariaco Basin is indicated by the blue box. A) ITCZ position in January, when insolation is at a minimum at 10° N. The ITCZ shifts south, creating a pressure difference that causes dry conditions and strong easterly winds over the Cariaco Basin, resulting in upwelling. B) ITCZ position in July, when insolation is at a maximum at 10° N. The zone of low pressure moves towards the Caribbean coast, resulting in high precipitation and runoff and a shutdown of trade winds and upwelling in the Cariaco Basin.
5
al., 2000a, 2000a), even though it also experienced more intense upwelling (González et al.,
2008b; Mertens et al., 2009a, 2009b). This pattern was driven by a combination of lower sea
level and a more southern ITCZ, which strengthened winter upwelling conditions. During the
Younger Dryas, a short period of cooling following the Last Glacial Maximum, the basin was
characterized by both high productivity and enhanced upwelling (Dahl et al., 2004; Herbert and
Schuffert, 2000; Werne et al., 2000), consistent with higher sea level and lower summer
insolation. Reduced rainfall during cold periods decreased forest cover on land (González et al.,
2008a; Hughen et al., 2004; Makou et al., 2007), consistent with a significant shift of the ITCZ to
a more southern location. This southward shift caused decreased rainfall in northernmost South
America and reduced terrestrial runoff to the basin during glacial periods compared to warmer
and wetter interglacials (González et al., 2008a; Haug et al., 2001; Peterson and Haug, 2006).
Cariaco Basin sediments may have also received increased aeolian inputs (Yarincik et al., 2000b)
during warm periods. Thus, terrestrial nutrient sourcing contributed to increased interglacial
productivity.
Sea level varied considerably both globally and in the Cariaco Basin between the MIS 8
glacial period and MIS 9 and 7 interglacials, with implications for the ventilation of the basin.
During the lowest point of the MIS 8 glacial period at 285 kya, sea level dropped up to 100 m
below current levels, before rising to 80 m below modern levels (Grant et al., 2014; Rohling et
al., 2009, 2009), although others estimate the decrease was -60 m (Siddall et al., 2007). During
the MIS 7 interglacial period, sea level rose, reaching 15 m lower than modern by 248 kya
(Dutton et al., 2009). In modern times, the deepest connections across the sill between the
Cariaco Basin and Caribbean Sea are 120 m across the north sill and 146 m on the western edge
6
(Richards and Vaccaro, 1956).
Figure 2: Map showing location of ODP site 1002 and modern bathymetry of the Cariaco Basin, adapted from Peterson et al. (2000b). The modern shoreline is highlighted in red, while a hypothetical MIS 8 maximal lowstand shoreline, assuming -100 m sea levels and modern bathymetry, is shown in blue.
The Cariaco Basin is a pull-apart basin formed through transtension between the El Pilar
and San Sebastian faults, transform faults that run East-West through the basin (Schubert, 1982)
and form part of the boundary between the South American and Caribbean plates. Much of the
subsidence in the Cariaco Basin occurred before early Pliocene, and since then tectonic activity
has shifted towards widening of the eastern lobe of the basin (Escalona et al., 2011). Given this
context, it is unlikely that the sill connections were substantially deeper in the Middle
Pleistocene. Thus it is possible that the Cariaco Basin's only access to the open ocean during
cold glacial periods was through a narrow channel less than 50 m (Figure 2).
This climate dynamic provides several important factors other than temperature that
could have substantially influenced phytoplankton populations. To better understand these
changes and their ecological impact, we coupled high resolution nannofossil assemblage data
7
with biomarker proxies for coccolithophore, diatom, and dinoflagellate productivity and organic
matter sourcing, as well as the alkenone unsaturation index sea surface temperature proxy, UK37'
(Brassell et al., 1986; Prahl and Wakeham, 1987). While other studies have reconstructed
phytoplankton communities in the Cariaco Basin using sterols (Dahl et al., 2004; Werne et al.,
2000) and alkenones (Herbert and Schuffert, 2000), none have examined the MIS 9-7 glacial
cycle. Likewise, Mertens et al. (2009b) presented data on calcareous nannofossil assemblages
but not during the MIS 9-7 glacial cycle. Thus we lack knowledge of phytoplankton during a
key interval for coccolithophore evolution: the global first appearance of Emiliania huxleyi,
previously identified for this site at 248 kya (Peterson et al., 2000b). The combination of
nannofossil and organic geochemical data allows us to assess how sea level, productivity,
temperature, and ecological interactions across both closely and distantly related phytoplankton
fostered range expansion and altered community composition.
Methods
Taxonomy
For nannofossil assemblages, smear slides were counted in cross-polarized light at 1600x
magnification. 300 specimens were counted per slide. This number was selected based on
rarefaction curves (Appendix A) that confirmed that 300 specimens are sufficient to capture the
diversity of the assemblages.
Taxonomy generally follows Okada (2000). Gephyrocapsa was split by size, into small
(2-3 µm), medium (3-5 µm), and large (> 5 µm) categories. We grouped these taxa in
Gephyrocapsa spp. Also following Okada, we separately counted "small placoliths", or
placoliths < 2 µm with thick rims. This category includes small Gephyrocapsa taxa variously
referred to as G. ericsonii (Hagino et al., 2000), G. protohuxleyi (McIntyre, 1970) and G. Minute
8
(Bollmann, 1997) as many specimens had recognizable bridges. This taxonomic grouping is
associated with tropical upwelling zones and has been used as a proxy for paleoproductivity
(Gartner, 1988; Kameo, 2002; Okada, 2000).
Emiliania huxleyi was combined with other, small placoliths (< 2µm) with a thin central
area and lacking a bridge. The miniscule E. huxleyi coccoliths are highly prone to dissolution,
and are difficult to distinguish in the light microscope from species such as Reticulofenestra
parvula. Similar approaches have been taken by other nannofossil workers (Cabarcos et al.,
2014; Rogalla and Andruleit, 2005). Additionally, recent phylogenetic work suggesting
extensive hybridization between Emiliania, R. parvula, and Gephyrocapsa (Bendif et al., 2016,
2015) casts further doubt on the evolutionary significance of dividing species within the
Noelaerhabdaceae. However, we continued to separate Emiliania and Gephyrocapsa due to their
distinct ecologies and biogeography in the modern ocean (Okada and Honjo, 1973).
Helicosphaera, Pontosphaera, and Syracosphaera were identified at the genus level only.
Other species counted were Florisphaera profunda, Rhabdosphaera claviger, Calcidiscus
leptoporus, Umbilicosphaera hulburtiana, and Reticulofenestra sp. >3 µm.
Stratigraphy and age model
Five holes (A-E) were drilled at Site 1002, which is located on the central saddle of the
basin (Figure 2), where seismic stratigraphy suggests continuous, flat deposition free from
slumping (Sigurdsson et al., 1997). Each hole was cored to 180 m, equivalent to 600 kyr, which
demonstrates an average sedimentation rate of 35 cm/kyr. The section consists of a single grey-
brown silty clay unit, which was divided into six subunits on the basis of microfossils. All of our
samples come from subunit E, a thick and homogenous greenish gray to dark greenish gray
9
nannofossil silty clay, with the exception of the lowermost 0.5 m, which come from subunit F, a
dark olive green nannofossil silty clay with diatoms. The carbonate content in the studied
interval averaged 27.8%.
10
We selected the core from Hole D for this study because it had better recovery over the
key interval previously identified as containing the first occurrence of Emiliania huxleyi, as well
as better sample availability relative to other cores. Sediments at Hole 1002D were correlated to
Hole 1002C, which has a published age model and is where most previous studies have been
10 20 30 40 50 60
320
300
280
260
240
sedimentation rate (cm/kyr)
age
(kya
)
9590
8085
Subunit E
Subunit F
dept
h (m
bsf)
MIS
9M
IS 8
MIS
7
Figure 3: Sedimentation rate and stratigraphy for studied interval of Hole 1002D. Green line indicates the boundary between lithologic subunit E and F.
11
conducted, using high-resolution magnetic susceptibility data (Sigurdsson et al., 1997). The
Hole 1002C age model (Peterson et al., 2000c) was based on oxygen isotopes of the planktonic
foraminifera Globigerinoides ruber and the SPECMAP time scale (Imbrie et al., 1984). The age
model had an average resolution of 7 kyr over the interval of this study, so dates were linearly
interpolated. Cores were correlated using the R package Astrochron (Meyers, 2014). The average
sedimentation rate in the dataset was 19.0 cm/kyr, with a minimum of 11.5 cm/kyr and a
maximum of 61.9 cm/kyr (Figure 3).
Organic Geochemistry Methods
Extraction methods for biomarkers generally follow the Bligh-Dyer method modified by
Wakeham and Pease (2004). Approximately 5 g of powdered, dry sediment were sonically
extracted three times in a 3:1 by volume azeotrope of dichloromethane and methanol. The total
lipid extract (TLE) was concentrated under N2 to near-dryness. One third of this lipid extract was
archived, and the remainder was saponified for two hours using 0.5 N potassium hydroxide in a
3:1 azeotrope of methanol to water. This mixture was then diluted to half its original
concentration with 5% NaCl, then extracted three times with hexane to produce the neutral
fraction. The pH of the aqueous phase was adjusted with hydrochloric acid to less than two, and
extracted three times with hexane to produce the acid fraction, which was archived without
analysis.
For sterol analysis, aliquots of the saponified neutral fraction were derivitized with
bistrimethylsilyltrifluoroacetamide (BSTFA) and pyridine for 60 minutes at 60° C to stabilize
sterols and alcohols. Androstane was added as internal standard to a concentration of 10 ng/µL.
The derivitized neutral fractions were analyzed on an HP 5973 mass spectrometer coupled to an
12
Agilent 6890 series gas chromatograph. Samples were injected with a splitless injection onto a
30 m, 0.25 mm diameter DB-XLB column with a 0.25 µm film thickness (Agilent), with an
initial oven temperature of 90°C followed by a ramp of 8°/minute to temperature of 300°,
followed by a ramp of 1°/minute to a final temperature of 320°. Sterols were identified by
retention time and mass spectra comparison to a standard mix consisting of cholesterol (Baker),
13% brassicasterol, 26% campesterol, 7% stigmasterol, 53% β-sitosterol (Indofine). Dinosterol
was identified by comparison to previously published retention order and spectra (Volkman et
al., 1990).
Alkenones were quantified on an Agilent 6890N gas chromatograph with Flame
Ionization Detection using splitless injection on a 60 m by 0.25 mm by 0.25 µm RTX-200 ms
column (Restek) at Brown University, with an initial oven temperature of 50°C held for 2 min,
followed by a 20°C/min ramp to 255° and then a ramp of 3°C /min to 320°C, held for 25 min, as
per Zheng et al. (2017).
Biomarker concentrations were calculated based on the ratio of peak areas to the internal
standard, androstane. Concentrations were converted to Mass Accumulation Rates (MARs) by
multiplying biomarker concentrations in nanograms per gram sediment times linear
sedimentation rates (cm/year; calculated for hole D based on the age model and correlation with
Hole 1002C) and bulk density (g/cm3) measured by the original ODP sampling party (Sigurdsson
et al., 1997).
Sea surface temperatures were estimated using the UK'37 alkenone unsaturation index
(Prahl and Wakeham, 1987):
UK'37 = C37:2/(C37:2+C37:3) (1)
13
Sea Surface Temperatures were calculated based on the calibration for Gephyrocapsa oceanica
(Volkman et al., 1995) because this species is numerically more abundant throughout the section
and in modern tropical environments than Emiliania (Okada and Honjo, 1973). The calibration
equation is as follows:
SST (°C) = (Uk’37 +0.520) × 20.41 (2)
These values were compared to mean daily insolation on February 15 at 10° N and
obliquity, which were calculated with the solution of Laskar et al. (2004) using the software
program Acycle (Li et al., 2019), as well as globally averaged trends in temperature and ice
volume via the LR04 benthic oxygen isotope stack (Lisiecki and Raymo, 2005).
Organic carbon, organic nitrogen, and carbonate contents were not measured in this
study. Instead, we employed previously measured values from Hole C (Haug et al., 1998). These
measurements were at a slightly higher resolution than our biomarker data set (average
resolution 2.1 vs. 4.6 kyr) and each point in our data was compared to the closest point in time.
Statistical Analyses
Statistical analyses, which included calculations of correlation and moving window
variance, were performed in R. All correlation coefficients given are Spearman's ρ, and all
correlations or regressions were performed on the first differences of the data to avoid spurious
relationships caused by temporal autocorrelation. Rolling variance was performed using the
package roll.
Pielou's evenness metric, J, which is defined as follows, was also calculated.
J = H/ln 𝑆 (3)
H is the Shannon Diversity Index, defined as
14
H = - 𝑝! ln𝑝!!!!! (4)
where pi is the proportional abundance of the ith species. S is the total number of species in the
assemblage. J ranges from 0 to 1, with higher values indicating a more even assemblage.
15
Results
Sea surface temperatures estimated from alkenones for Site 1002 followed global
temperature trends (Figure 4); the gradual cooling trend from MIS 9 into MIS 8 was interrupted
by transient warming around 280 kya. After a temperature minimum of 21.4° C at 260 kya, sea
surface temperatures increased rapidly to a maximum of 27.7° at 242 kya. These trends slightly
lead but generally follow the LR04 stack, which represents globally averaged deep sea
temperature and ice volume. Trends in SST generally followed winter (February 15) insolation,
370 400
320
300
280
260
240
insolation (W/m2
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37 alkenone unsaturation index. Marine Isotope Stage (MIS) chronology is indicated on the right.
16
which makes sense as winter insolation determines the strength of the southern ITCZ and the
strength of upwelling in the Cariaco Basin.
Plate 1: SEM micrographs of morphologies that would be identified as E. huxleyi/R. parvula in the light microscope. A-C from 241.0 kya, proximal view; A and B show Emiliania huxleyi sensu stricto features, notably a perforated, "hammerhead" distal shield. A-C are almost identical in proximal view. D-F from 250.9 kya; the thick inner cycle of D coupled with thin rim would closely resemble Emiliania huxleyi sensu stricto in the light microscope. G-I from 300.7 kya, showing morphology typical of R. parvula.
SEM imaging (Plate 1) revealed that many specimens that resembled Emiliania huxleyi in
the light microscope were likely in actuality Reticulofenestra parvula, a closely related species.
Henceforth, we refer to these two species together as the Emiliania complex. Preservation was
17
adequate but not perfect throughout this section (Appendix 2), and if dissolution removed the
fragile distal shield of E. huxleyi, it would leave only the thicker inner rim and the specimen
woul resemble R. parvula (Plate 1H), as the proximal shields of the two are virtually identical
(Plate 1B, C). The only unambiguous specimens of E. huxleyi were observed at 241.0 kya.
Nevertheless, there is a substantial increase in the relative abundance of the Emiliania complex
(Figure 5), which suggests a shift in ecology in the group.
Figure 5: Relative abundances of calcareous nannofossil and Pielou's evenness metric
Nannofossil abundances broadly follow ocean temperatures. While the 280 kya warming
was not accompanied by change in nannofossil assemblage data, the warming at 260 kya
correlated with pronounced upheaval in the abundance of all major nannofossils (Figure 5).
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MIS 7
MIS 9
Emiliania complex small placoliths F. profunda
Gephyrocapsa spp. other
320
300
280
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18
Notably at this time, the Emiliania complex, a minor component of the assemblage for most of
the studied interval, increased in abundance to 30%. The abundances of other major components
of the assemblage, the small placoliths, Florisphaera, and Gephyrocapsa, fluctuated
significantly. As seen in Figure 6, temporal variability in nannofossil abundances increased
during this interval.
19
Mass Accumulation Rates of all major biomarker classes increased during warm periods
but individual compound groups followed the trend of SST and global δ18O values to different
degrees (Figure 7). Brassicasterol and brassicastanol, two structurally similar biomarkers for
diatoms were highly correlated (ρ = 0.834) with each other and the pair are hereby referred to as
diatom sterols. This compound group had the strongest relationship with temperature. Dinosterol,
by far the most abundant of any biomarker here, weakly correlated with temperature and other
Figure 6: Rolling variance for major coccolith species, excluding the Emiliania complex, which sharply increases following the temperature minimum at 260 kya. Calculated with a rolling window with a constant n=7. Since temporal resolution decreases with age in this data set, the amount of time averaged decreases up section.
0.00 0.02 0.04 0.06 0.08
320
300
280
260
240
age
(kya
)
small placoliths
F. profunda Gephyrocapsa spp. other
MIS 7
MIS 8
MIS 9
20
biomarkers. Similarly, the combined abundances of C37:2 and C37:3 alkenones (hereafter referred
to as ΣC37) weakly correlated with alkenone temperature estimates. Additionally, dinosterol
strongly correlated with the relative abundance of F. profunda. None of the biomarkers
correlated well with other nannofossil taxa, % total organic carbon (TOC), or each other (Table
2).
Table 1: First differences correlations (ρ) between phytoplankton biomarkers, paleoceanographic proxies, and nannoplankton relative abundances.
SST (°C)
β-sitosterol MAR
% TOC Emiliania complex relative abundance
small placoliths relative abundance
F. profunda relative abundance
Gephyro-capsa spp. relative abundance
dinosterol MAR
0.160 0.276 0.126 -0.211 -0.205 0.537 -0.375 ΣC37
MAR 0.199 0.201 -0.117 0.103 0.291 -0.007 0.216
Brassica-stanol + stenol MAR
0.564 0.912 0.122 0.103 0.456 -0.015 -0.401
Table2:First differences correlations (ρ) between phytoplankton biomarkers.
dinosterol MAR ΣC37 MAR Brassicastanol + stenol MAR
dinosterol MAR 1 -- -- ΣC37 MAR 0.170 1 -- Brassicastanol + stenol MAR
0.375 0.062 1
21
Figure 7: Percent organic carbon and biomarker Mass Accumulation Rates. Left: Alkenone unsaturation index (UK
37') estimates of Sea Surface Temperature (SST). Second Left: Percent Total Organic Carbon (Haug et al., 1998). All others are Mass Accumulation Rates (MAR) of different biomarker classes: Dinosterol is a C30 sterol produced by dinoflagellates. Total C37 alkenones (the sum of the C37:3 and C37:2 alkenones) are produced by Noelaerhabdacean haptophytes, likely Gephyrocapsa. Brassicasterol and brassicastanol, a diagenetic product of the former, are both C28 stenols associated with diatoms, particularly Thalassiosira. β-sitosterol is a C29 sterol produced predominantly by plants and interpreted as a marker for terrestrially-sourced organic matter.
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22
Discussion
This data set shows evidence for profound changes in ecology and climate between cool
and warm periods in the Cariaco Basin.
Ecological Response of taxa to environmental change
Figure 8: Schematic showing ecological response of major phytoplankton clades in accordance with Margalef's Mandala. Top right shows a rendering of Margalef's Mandala (Margalef 1978) with proxies used in this study superimposed on the plot. F. profunda relative abundance is a proxy for turbulence and β-sitosterol mass accumulation rate as a proxy for nutrient inputs. Smaller plots cross plot the first differences of proxies for phytoplankton clades against these proxies: A) diatoms (brasssicastanol+stenol) vs. nutrient input (β sitosterol); coccolithophores (C37 alkenones) vs nutrient input (B); and coccolithophores (C37 alkenones) vs stratification (% F. profunda (C); and D) dinoflagellates (dinosterol) vs. stratification (% F. profunda).
23
While all indicators for phytoplankton groups responded to ocean warming, each major
plankton group responded differently and in accord with existing models of their ecology (Figure
8). Margalef's Mandala (Margalef, 1978) delineates a classic view of the ecological preferences
of the three main plankton groups: dinoflagellates occupy a low turbulence, low nutrient zone,
diatoms a high turbulence, high nutrient zone, and coccolithophores an intermediate zone for
both properties. Taking plant-derived β-sitosterol MAR as a proxy for terrestrial-sourced nutrient
inputs, and the relative abundance of F. profunda as a proxy for stratification, our data support
the Margalef model of ecological response as a key driver of changes in abundance of the three
types of phytoplankton over time. Diatom sterol and β-sitosterol MARs were highly correlated
while dinosterol (Table 1), suggesting that diatom production of organic matter was strongly
driven by surface nutrient inputs. Total C37 alkenone abundances were uncorrelated with β-
sitosterol, consistent with an intermediate or generalist affinity to nutrient inputs. In contrast,
dinosterol MARs were highly correlated with F. profunda relative abundance, suggesting that
dinoflagellate production was enhanced during warm periods characterized by stratified
conditions. F. profunda is a deep thermocline dweller that has been used as a proxy for
stratification and a deep nutricline (McIntyre and Molfino, 1996; Molfino and McIntyre, 1990)
or turbidity (Ahagon et al., 1993). In contrast, diatom and coccolithophore biomarker MARs
showed little relationship with F. profunda abundance, suggesting insensitivity to stratification.
Biomarker mass accumulation rates suggest that dinoflagellates were the principle
primary producers throughout MIS 9-7 and especially during interglacials. These findings are
consistent with the results of González et al. (2008b), who observed an increase in autotrophic
dinocysts during interglacials in MIS 3-4 and interpreted this as a signal of stratification. We
caution that β-sitosterol can be produced by certain diatoms (Volkman, 1986), complicating this
24
interpretation. Further work should incorporate additional proxies for plant organic matter such
as n-alkanoic acids in order to verify the relationship between diatoms and terrestrial inputs.
In contrast to other nannofossil taxa, the Emiliania complex shows a monotonic increase
with little short-term variation despite dramatic environmental changes in the 250-240 kya
warming period. This supports an interpretation of Emiliania as a generalist (as opposed to an
obligate eutrophic taxon)-- the exact ecology one would expect this variable environment to
select for. This is consistent with a range of modern studies (Andruleit and Rogalla, 2002; Balch,
2004; Ziveri and Thunell, 2000) that have shown insensitivity or contrary response to upwelling.
As Emiliania does not have a consistent relationship with upwelling, we recommend against its
use as a proxy for upwelling (e.g. Mertens et al., 2009b; Stoll et al., 2007).
Glacial/Interglacial trends in productivity and nutrient sourcing
The large increase in biomarker mass accumulation rates starting around 250 kya
suggests an increase in productivity and nutrient delivery to the Cariaco Basin, but what was the
mechanism for this? While biomarker compound mass accumulation rates generally increased
with warmer temperatures, only diatom sterols have a meaningful correlation with temperature,
suggesting other oceanographic factors may also be significant. Anomalously low SSTs at 260
kya coupled with very low relative abundances of F. profunda suggest upwelling was enhanced
in the basin during this cold period. This is consistent with previous models (González et al.,
2008b; Mertens et al., 2009a, 2009b) and suggests a stronger southern winter ITCZ during
glacial periods. This is supported by modern climatology: southern displacement of the ITCZ
position during winter months fosters upwelling conditions in the Cariaco. The paradox that
more intense upwelling was coupled with indicators of low productivity such as lower biomarker
25
accumulation rates during glacial periods can be resolved by isolation of the Cariaco Basin from
Caribbean waters. During the glacial low stand, the shallow sill (Tortuga Bank) was more
exposed such that only a shallow conduit connected the basin to the open ocean. With depths
less than 70 m, only nutrient-depleted surface waters could be delivered to the basin across the
shallow connection to the open ocean (Haug et al., 1998; Peterson et al., 1991). The lack of
connectivity restricted marine nutrient delivery during cool periods and resulted in lowered net
production. As the sill prevents lateral advection of deep marine waters both today and in the
past, any water upwelled in the Cariaco Basin during glacial periods would have been recycled
from nutrient-depleted water sourced from the basin itself.
During warm periods, increased terrestrial inputs suggest a mechanism for increased
productivity. The concentration of β-sitosterol, a compound produced by higher plants (Meyers,
1997) increased during interglacials and the transient 280 kya warming period. This suggests
increased terrestrial runoff carried more organic matter and nutrients into the basin during
warmer times. This scenario is consistent with evidence from pollen and n-alkanes for increased
forest cover (González et al., 2008a; Hughen et al., 2004), and evidence from sedimentology
(Peterson and Haug, 2006) for increased riverine fluxes, that together reinforce that interglacials
the northern reaches of South America were wetter, likely due to a more northern ITCZ
enhancing the summer climate regime.
During the warming period from 250-240 kya, the basin was more stratified than it was
during glacial maximum, as evidenced by increased abundances of F. profunda, a proxy for
stratification, and stronger terrestrial signals. However, nannofossil abundances during the 250-
240 kya interval were highly variable, including the small placolith group. This taxa has been
considered an upwelling or eutrophication indicator (Gartner, 1988; Kameo, 2002; Okada, 2000).
26
In our data, they had a strong anti-correlation (ρ = -0.606) with Florisphaera. This anti-
correlation suggests alternating periods of stratification and upwelling fueled variability in
productivity.
This inferred alternation between stratification and mixing induced high turnover in the
entire nannofossil assemblage, creating a rapid succession of taxa during the 250-230 kya
interval. The same ecological variability and intense stratification was not observed during the
InterglacialGlacial
Inte
rpreta
tion
Evid
ence
sill sill
Lower β sitosterol MAR
Lower sedimentation rate
Lower biomarker accumula-tion rates
Lower F. profunda abun-dance
Anomalously low SSTs
Higher β sitosterol MAR (pollen & plant waxes from other interglacials)
Higher sedimentation rate
Lower biomarker accumula-tion rates
Higher F. profunda abun-dance
Increased connectivityHigher freshwater inputs
More nutrient inputsStratifiedMore intense July ITCZ
RestrictedHigher salinityNutrient StarvedUpwelling Enhanced
More intense Feb. ITCZ
Figure 9: Schematic summarizing interpretation of glacial-interglacial environmental changes.
27
earlier (280 kya) warm period, although this may be a product of lower temporal resolution in
the nannofossil data set over this interval. This suggests that in this climate regime, rapid
warming increases variability and causes a shift between stratified and mixed oceanographic
modes, destabilizing the entire phytoplankton ecosystem.
Biomarkers and Nannofossils: a new approach for paleoceanographic reconstruction
Integrating biomarker and nannofossil proxies presents a promising approach for
paleoceanographic reconstruction. However, a comparison between two different methods of
reconstruction underscores the limitations that come from differences in the nature of the
proxies. Coccolithophore ecology was evaluated using two different methods: total C37
alkenones, which in marine environments are produced by coccolithophores in the family
Noelrhabdaceae, principally G. oceanica and E. huxleyi, and calcareous nannofossil relative
abundances. The ΣC37 alkenone accumulation rate had no correlation with either G. oceanica or
the E. huxleyi complex, emphasizing that production or preservation biases influence organic and
inorganic forms differently. Of all nannoplankton species, alkenones had the strongest
correlation with Calcidiscus leptoporus (ρ = 0.443), an unrelated species that is not known to
produce alkenones today. This points to the complications of comparing these disparate metrics
of similar phenomena; relative abundance versus mass accumulation rates. Additionally, not all
genotypes of alkenones-producers create coccoliths (Rokitta et al., 2011), so coccolith counts
may not accurately estimate alkenone production. This is consistent with previous studies that
have shown that the UK'37 alkenone proxy is not sensitive to nannofossil assemblage changes
(Jordan et al., 1996; McClymont et al., 2005).
28
Due to production and dissolution biases, nannofossil assemblages may not be
representative of total production, but they still provide a wealth of information about relative
changes in oceanography that biomarkers cannot. Biomarker proxies for temperature and organic
matter sourcing are well developed, but are seasonally and spatially averaged in marine settings.
Nannofossil proxies can provide additional valuable information about water column structure.
Combining both provides a more complete, three-dimensional reconstruction of
paleoceanographic variables than either alone.
The sterol biomarker approach to community reconstruction, while coarse, allows for
comparison across disparate clades, moving us closer to an accounting of the ecology of the
entire phytoplankton community. Diatom, dinoflagellate, and coccolithophore microfossils all
have different production and preservation conditions, making quantitative comparisons difficult,
and these conditions vary considerably even within coccolithophores (Young et al., 2005). Our
combined biomarker-nannofossil approach shows promise in elucidating the ecologies of extinct
species of calcareous nannofossils, particularly if combined with multivariate methods. Placing
extinct species in a turbidity- surface productivity space, as we have done here, could help test
hypotheses about important problems such as mechanisms for floral change during other periods
global change (i.e. Eocene hyperthermals) or long term trends in diversity among other algal
clades. These methods should be applicable throughout the Cenozoic, as the long-ranging
Cenozoic genus Discoaster was replaced by F. profunda and is thought to share a similar
ecology (Schueth and Bralower, 2015).
29
Sea level change, biogeography, and dispersal of E. huxleyi
Due to taxonomic uncertainty, we cannot definitively identify the first occurrence of
Emiliania huxleyi sensu stricto, but the sudden increase in abundance at 250 kya is highly
suggestive of dramatic ecological or evolutionary changes. This increase should not be confused
with the Emiliania huxleyi acme, which occurred diachronously at 60-80 kya (Raffi et al., 2006;
Thierstein et al., 1977), above the interval of this study. Globally, E. huxleyi first occurred at
open ocean sites at 291 kya (Raffi et al., 2006), later than our first observations of the Emiliania
complex (at 330.1 kya) but earlier than this increase in abundance. Our SEM images provided no
evidence for Emiliania huxleyi, sensu stricto, from 250.9 kyr or before (Plate 1). Previous work
at Site 1002 (Peterson et al., 2000b) also reported the first occurrence of Emiliania huxleyi at 248
kya. Two explanations are compatible with the later appearance: either environmental instability
associated with warming at 250 kya allowed Emiliania huxleyi, sensu stricto, a previously rare
species, to become more abundant, or sea level rise at 250 kya introduced E. huxleyi s.s. into the
Cariaco Basin for the first time.
The first, ecological hypothesis posits that Emiliania huxleyi, sensu stricto was
cryptically introduced somewhere near the global datum, but only became ecologically important
once climate warmed and disrupted the coccolithophore ecosystem around 250 kya. The increase
in abundance of the Emiliania complex at 250 kyr coincides with dramatic changes in circulation
and nutrient supply that led to high variability in the abundance of other nannofossil taxa.
Emiliania is a generalist (Andruleit and Rogalla, 2002; Balch, 2004; Ziveri and Thunell, 2000;
this study), an ecology that would have succeeded in this highly variable, unstable environment.
Alternately, E. huxleyi s.s. may have been introduced late, around 250 kya, due to
biogeographic barriers. Studies of Emiliania huxleyi first occurrences elsewhere (Rio et al.,
30
1990; Sato et al., 1991; Thierstein et al., 1977) generally observed a sudden first appearance, as
opposed to the long period of rarity before a sudden increase we see in the Emiliania complex. If
E. huxleyi s.s. followed this a similar in the Cariaco Basin, the long period of rarity may have
consisted solely of R. parvula or similar forms, while the sudden increase corresponded to the
first occurrence of E. huxleyi s.s.
Specifically, reduced connectivity with the open ocean due to lower sea level delayed the
dispersal of Emiliania huxleyi sensu stricto into the basin. During the MIS 8 glacial period, sea
levels likely ranged between -80 m and -100 m below modern (Grant et al., 2014), and the
Cariaco Basin is separated from the Caribbean Sea by a 100 m deep sill, which would have
restricted marine inflow to two narrow channels <50 m deep, with some very shallow flow over
the sill depending on the magnitude. During glacial periods, the smaller volume of ocean water
reaching the Cariaco Basin due to low sea levels would have statistically reduced the likelihood
that new coccolithophore populations would reach the basin in sufficient abundance to compete
with established populations. As sea level rose, the volume of exchange with the open ocean
increased concurrently with other changes in the oceanographic regime.
Under 80-100 m sea level changes, the Cariaco Basin would not have been fully isolated
but would have experienced reduced marine influence, decreasing the likelihood of introducing
new populations and altering oceanographic conditions (discussed in the the previous section).
Reduced connectivity to the Caribbean Sea would have reduced nutrient inputs and increased
salinity (Peterson et al., 1991), due to decreased marine and freshwater input in a tropical
environment with high evaporation. Nannofossil relative abundances (Figure 5,6) were not
sensitive to the onset of lower sea level but changed dramatically when the climate warmed and
sea level rose. Sea level fell much more gradually than it rose (e.g., Grant et al., 2014), so the
31
lack of response to slow cooling suggests that coccolithophores are able to adapt to a range of
conditions if the pace of change is slow, but not to swifter changes. Modern coccolithophore
community composition in the Arabian Sea, which has variable upwelling and salinity ranges
similar to the Cariaco Basin, is controlled more by productivity than by salinity (Andruleit and
Rogalla, 2002), so we suggest that the changes in community composition at 250 kyr in the
Cariaco Basin were similarly driven by rapid changes in the volume and spatial distribution in
nutrient inputs rather than lowered salinity. While cell abundance in modern E. huxleyi is not
sensitive to salinity, coccolith number and shape is (Bollmann and Herrle, 2007; Fielding et al.,
2009). However, while E. huxleyi produces larger and more abundant coccoliths under higher
salinity conditions, compelling evidence for E. huxleyi s.s. appears in the Cariaco Basin
concurrently with interpreted decreases in salinity, suggesting that the trend is not caused by
salinity-driven preservational differences.
Disentangling the influence of sea level and climate changes associated with sea level
remains a challenge in paleoceanography, as does disentangling ecological and environment in
paleobiology. Despite these limitations, the hypothesis that E. huxleyi s.s. emerged later in the
Cariaco Basin than in the open ocean due to the basin's relative isolation during glacial periods is
consistent with all available evidence.
Implications for future change
In this study, we found that warming in the Cariaco Basin was associated with increased
productivity and nutrient loading, and ecological variability in phytoplankton. While some of
these changes were enhanced by insolation and may not directly apply to anthropogenic climate
change, others may be of use for policymakers and forecasters of the future. In particular,
32
stratification and nutrient delivery in tropical coasts like the Cariaco Basin may be enhanced by
future warming due to higher sea surface temperatures and increased precipitation (Hallegraeff,
2010; Wells and Karlson, 2018). These temperature-triggered stressors may be further enhanced
by anthropogenic nutrient loading and land use changes that increase erosion (Glibert et al.,
2018). An important takeaway from this study is that intervals of rapid warming coincide with
highly variable environments which lead to ecological variability in phytoplankon. Together with
the enhanced prospects for introduction of new populations due to sea level rise, this heightened
variability and nutrient loading may increase the frequency of harmful algae blooms in
environments like the Cariaco Basin.
33
Conclusions
Both calcareous nannofossil and organic geochemical reconstructions of plankton
ecosystems in the Cariaco Basin showed pronounced ecological disruption following the
initiation of rapid warming at 250 kya, but were fairly insensitive to gradual cooling or lower-
amplitude warming earlier in MIS 8. This coincided with the introduction of Emiliania to the
Cariaco Basin, which was enabled by rising sea level and increased connection to the open
ocean. Sea level rise and increasing terrestrial runoff increased both productivity and
stratification, consistent with a more intense northern ITCZ during interglacial periods. Overall,
the period of warming at the end of MIS 8 was characterized by increased environmental
variability. The entire interval was dominated by dinoflagellate productivity.
34
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Appendix A
Rarefaction analysis of nannofossils
To validate sampling choices, species accumulation curves (Figure A1) were constructed
by counting 500 individuals for three samples, two with similar high diversity and one with
visibly lower diversity from Hole D. The species richness was recorded every 25 and then 50
individuals. These curves were compared to rarefaction curves estimated from the maximum
diversity, created using the iNEXT package (Hsieh et al., 2016) using the R software platform
version 3.4.1 (R Core Team, 2017) . Bootstrap-based 95% confidence intervals (Chao et al.,
2014) confirm that the two samples in the same interval have the same diversity, which differs
from the third sample.
This comparison demonstrates that both higher and lower diversity samples in this core
have leveled off in species accumulation by 200 individuals, so counting to this level captures
most of the diversity in the assemblage. One caveat is that the distribution of species in these
assemblages is fairly skewed (Figure 2). On the one hand, this means that the most significant
contributors to the assemblage will definitely be captured by counting to 200. On the other hand,
rare constituents will likely be missed this way. Fortunately, the taxa of interest to this study,
Geophyrocapsa, Emiliania, and Florisphaera, are all relatively abundant, and together make up
35-65% of each assemblage.
42
Figure A1: Rarefaction (solid line) and species accumulation (dotted line) curves for calcareous nannofossils. The y-axis shows species richness. Shaded regions indicate a 95% confidence interval around rarefaction curves.
0 100 200 300 400 500
24
68
1012
14
10H3W_109-11110H3W_74-769H5W_68-69
Number of individuals
Spe
cies
div
ersi
ty
43
Figure A2: Nannofossil Rank-abundance curves, counted to 500.
References: Chao, Anne, Nicholas J. Gotelli, T. C. Hsieh, Elizabeth L. Sander, K. H. Ma, Robert K. Colwell, and Aaron M.
Ellison. 2014. “Rarefaction and Extrapolation with Hill Numbers: A Framework for Sampling and Estimation in Species Diversity Studies.” Ecological Monographs 84 (1): 45–67. https://doi.org/10.1890/13-0133.1.
Hsieh, T. C., K. H. Ma, and Anne Chao. 2016. “iNEXT: An R Package for Rarefaction and Extrapolation of Species Diversity (Hill Numbers).” Methods in Ecology and Evolution 7 (12): 1451–56. https://doi.org/10.1111/2041-210X.12613.
R Core Team. 2017. R: A Language and Environment for Statistical Computing. Vienna, Austria.
rank
abundance
0
50
100
150
200
250
5 10 15
10H3W_109-111 10H3W_74-760
50
100
150
200
250
9H3W_68-69
44
Appendix B
Nannofossil preservation
Preservation of nannofossil samples was evaluated using the Gephyrocapsa bridge index,
defined as the percentage of Gephyrocapsa (> 2.5 um) with an intact bridge. Specimens with
bridges visible upon rotation of 90°, regardless of thickness, were considered intact, while
specimens with partial, disconnected bridge fragments were not considered intact. This metric
was used for several reasons: Gephyrocapsa is easy to identify and is abundant throughout the
assemblage, consisting of >20% of the assemblage in all samples. Gephyrocapsa is dissolution-
resistant (Roth and Coulborn 1982) and obviously broken, as opposed to etched, coccoliths were
rare throughout samples. The Gephyrocapsa bridge appears to be more sensitive to dissolution.
Finally, the Gephyrocapsa bridge is an important taxonomic distinguisher, and etched, bridgeless
Gephyrocapsa can resemble Reticulofenestra or Emiliania, so the bridge index is also a metric of
taxonomic uncertainty.
Coccolith preservation was relatively constant and moderate through the interval, and
was not related to oceanographic changes. Note however that two points of exceptionally poor
preservation at 250 and 240 kya correspond to peaks in the abundence of Florisphaera. Overall,
Florisphaera relative abundance and preservation have a moderate inverse correlation (detrended
ρ = -0.464), consistent with the observation that Florisphaera is a dissolution-resistant species
(Fernando et. al 2007). Nevertheless, the Florisphaera signal is not solely due to dissolution, as
it correlates with geochemical signals independent of dissolution, such as dinosterol, in a
coherent fashion and the 250 kya increase in Florisphaera abundance is accompanied by an
increase in the abundance of the Emiliania group, a highly dissolution-prone morphology.
45
Figure B1: Coccolith preservation over time for the sampled interval.
References Fernando, Allan Gil S., Alyssa M. Peleo-Alampay, Edward S. Lucero, and Martin G. Wiesner. 2007. “Surface
Sediment Distribution of Florisphaera Profunda in the South China Sea: An Effect of Dissolution?” Journal of Nannoplankton Research 29 (2): 102–7.
Roth, Peter H., and William T. Coulbourn. 1982. “Floral and Solution Patterns of Coccoliths in Surface Sediments of the North Pacific.” Marine Micropaleontology 7 (1): 1–52. https://doi.org/10.1016/0377-8398(82)90014-7.
20 40 60 80
320
300
280
260
240
Gephrocapsa bridge index
age
(kya
)
46
Appendix C
Multivariate Analysis
Nannofossil assemblages were analyzed using Nonmetric Multidimensional Scaling
(NMDS) and Detrended Correspondence Analysis (DCA), two ordination methods used to
visualize variation within a data set. NMDS is a brute force ordination method that randomly
assigns points to the ordination space, then moves them to minimize stress, a quantity which
measures the non-monotonicity of the difference between the original and ordination space. This
NMDS converged after twenty tries, with a maximum allowed of 50. DCA is an ordination
method based on reciprocal averaging that is often used for ecological data sets because it avoids
treating samples with absent species as similar in composition, and it is distinguished from
Correspondence Analysis by the inclusion of a detrending algorithm that avoids certain artifacts.
Both ordinations used the Bray-Curtis dissimilarity metric, and all analyses were performed in R
using the package vegan (Oksanen et al., 2018).
In order to fit to sample attributes, the nannofossil assemblage relative abundance data
discussed in the main text was subset to data points that included corresponding measurements
from the biomarker data set. As data were highly skewed, a logarithmic transform was applied to
the data, which was then standardized by the column maximum. Environmental data, which
includes the biomarker data discussed in the text as well as carbon and nitrogen data from Haug
et al. (1998), was standardized to a mean of zero and standard deviation of one to account for the
diverse scales and data types in this data set. These data were fit to the ordinations using the
function envfit from vegan.
47
Figure C1: NMDS of nannofossil data set, with fitted environmental variables
-0.6 -0.4 -0.2 0.0 0.2 0.4 0.6
-0.6
-0.4
-0.2
0.0
0.2
0.4
NMDS1
NMDS2
age
sedrate
desmosterol
diatoms
B.sitosteroldinosterol
cholesterol
C37
llSST
CaCO3
TOC
C/N
δ15N
cholesterol
desmosterol
48
Figure C2: DCA of nannofossil data set, with fitted environmental variables.
None of the available environmental variables clearly explain the axes of variance in the
NMDS, though axis 1 may correspond to SST, and axis 2 corresponds to percent carbonate. In
DCA, DCA1 likely represents a productivity variable, as it aligns well with total organic carbon
and β-sitosterol (terrestrial inputs). Curiously, these two are anti-correlated, which is surprising,
as we would expect productivity (and thus sedimentary organic carbon) to increase with
terrestrial nutrient delivery. Axis 2 may correspond to stratification, as it corresponds well with
-1.0 -0.5 0.0 0.5 1.0
-1.0
-0.5
0.0
0.5
DCA1
DCA2
agesedrate
cholesterolB. sitosterol
desmosteroldiatoms
desmostanol
C37
SST
CaCO3
TOC
C/N
δ15N
dinosterol
cholesterol
49
δ15N. The δ15N signal has previously been interpreted as predominantly driven by denitrification
(Haug et al. 1998), which occurs at the oxic-anoxic interface under stratified conditions. This is
largely consistent with our conclusions from the main body of the paper.
Reference:
Oksanen, J., Blanchet, F.G., Friendly, M., Kindt, R., Legendre, P., McGlinn, D., Minchin, P.R., O’Hara, R.B., Simpson, G.L., Solymos, P., Stevens, M.H.H., Szoecs, E., Wagner, H., 2018. vegan: Community Ecology Package.
51
sample
ageC
_37:4 m
ethylC
_37:3 m
ethylC
_37:2 m
ethylC
_38:3 ethyl
C_38:2 ethyl
desmo-
steroldesm
o- stanol
chole- sterol
chole- stanol
brassica- sterol
brassica-stanol
B
sitosteroldinosterol
9H5W
_113-115233.3
BD
L21.70
51.5932.54
40.182.58
0.86304.46
2.826.13
3.188.01
194.039H
6W_64-66
239.3B
DL
25.5451.79
30.7944.47
1.621.41
186.506.45
5.405.25
5.391686.49
9H6W
_104-106241.0
BD
L23.14
50.7919.02
44.426.56
2.27461.26
6.849.23
4.088.08
433.069H
6W_138-140
242.4B
DL
18.7097.82
15.5868.41
9.235.67
598.8118.12
16.8212.65
11.48575.77
9H7W
_26-28244.1
BD
L23.79
77.2921.98
66.125.78
2.81368.03
9.449.90
5.926.80
838.139H
7W_75-77
246.2B
DL
32.2096.72
32.0686.52
3.382.20
315.018.23
8.486.92
5.11522.78
10H2W
_14-16249.3
BD
L0.57
1.912.91
2.292.94
5.28239.03
13.837.93
12.046.59
282.9210H
2W_114-116
254.5B
DL
15.1032.64
18.5633.17
0.540.56
59.361.36
0.821.26
0.6161.06
10H3W
_1-3256.7
BD
L34.06
44.6442.23
23.590.28
0.3429.97
1.070.63
1.130.46
76.4510H
3W_44-46
259.5B
DL
21.5924.38
21.0825.70
0.150.16
18.950.48
0.390.37
0.2827.03
10H3W
_109-111265.1
BD
L24.21
28.7829.84
28.210.19
0.1521.56
0.490.55
0.490.24
39.0710H
3W_139-141
267.7B
DL
45.3361.32
39.0264.13
0.330.33
35.320.87
1.080.93
0.6368.47
10H5W
_9-11277.1
BD
L51.29
112.4355.76
23.592.28
2.91178.02
7.125.36
6.404.36
249.9110H
5W_49-51
279.0B
DL
55.49169.35
49.37147.42
3.696.52
323.5319.66
10.0015.36
8.70459.56
10H5W
_129-131283.0
BD
L43.28
158.2347.01
127.953.16
5.19265.47
13.877.58
12.006.70
549.9210H
6W_79-81
288.6B
DL
40.7279.88
51.2012.56
0.571.14
47.152.75
1.732.47
1.56159.76
10H6W
_109-111290.1
BD
L13.78
27.8418.08
4.690.87
1.31122.11
3.242.41
3.692.73
12.5710H
7W_29-31
292.8B
DL
49.48129.36
51.0817.98
2.373.80
191.389.64
5.837.09
5.06292.18
10H7W
_129-131297.7
BD
L85.44
216.9178.65
202.642.00
2.83228.70
6.195.36
5.334.71
299.3911H
1W_36-38
300.7B
DL
24.2671.18
19.1860.88
2.885.38
239.3413.89
7.3110.78
7.24515.05
11H4W
_23-27322.5
BD
L58.25
227.6762.32
174.225.59
5.29351.85
13.409.28
10.188.62
501.1911H
5W_48-50
330.9B
DL
43.27117.87
46.0197.82
6.130.88
419.112.78
5.011.28
6.10145.94
Table D1: Concentrations for measured biomarkers. C_37 etc. are all akenones. C37:4 was near detection limit for samples, and so concentrations are not reported
52
Table D2: Parameters used in mass accumulation rate calculations. Density is Boyce corrected density based on gamma ray attenuation porosity (Sigurdsson et al., 1997). Sediment mass is the dry mass of extracted sediment.
sample age depthsedimentation rate (cm/kyr)
density (g/cm^3)
sediment mass (g)
Fraction injected
(alkenones)
Fraction injected (sterols)
9H5W_113-115 233.3 78.01 14.50 1.65 5.016 0.00634 0.006349H6W_64-66 239.3 79.03 26.58 1.228 5.09 0.00634 0.006349H6W_104-106 241.0 79.42 29.00 1.453 5.07 0.00634 0.003179H6W_138-140 242.4 79.76 24.17 1.795 5.06 0.00634 0.006349H7W_26-28 244.1 80.16 24.17 1.636 5.04 0.00634 0.006349H7W_75-77 246.2 80.65 21.75 1.662 5.02 0.00634 0.0063410H2W_14-16 249.3 81.41 24.17 1.682 4.98 0.00507 0.0031710H2W_114-116 254.5 82.41 18.13 1.738 4.95 0.00634 0.0031710H3W_1-3 256.7 82.81 18.13 1.516 4.98 0.00634 0.0031710H3W_44-46 259.5 83.21 11.50 1.534 5.09 0.00634 0.0031710H3W_109-111 265.1 83.86 11.50 1.598 5.06 0.00634 0.0031710H3W_139-141 267.7 84.16 11.50 1.5 4.96 0.01011 0.0031610H5W_9-11 277.1 85.86 24.37 1.691 5.01 0.00688 0.0034410H5W_49-51 279.0 86.26 20.38 1.708 5.09 0.00677 0.0033910H5W_129-131 283.0 87.06 20.38 1.388 4.99 0.00653 0.0032710H6W_79-81 288.6 88.2 20.34 1.586 5.01 0.00600 0.0060010H6W_109-111 290.1 88.5 20.34 1.674 5.04 0.01105 0.0025110H7W_29-31 292.8 89.06 24.44 1.527 5.01 0.00634 0.0063410H7W_129-131 297.7 90.06 22.40 1.287 5.00 0.00923 0.0023111H1W_36-38 300.7 90.56 16.07 1.413 4.99 0.00655 0.0065511H4W_23-27 322.5 94.06 16.13 1.752 4.99 0.00634 0.0063411H5W_48-50 330.9 95.81 59.47 1.409 5.02 0.00685 0.00685
53
Sample
ageC
37:4 m
ethylC
37:3 m
ethylC
37:2 m
ethylC
37:3 ethyl
C38:2
ethyldesm
o- sterol
desmo-
stanolchole- sterol
chole- stanol
brassica- sterol
brassica- stanol
B
sitosteroldinosterol
9H5W
_113-115233.3
NA
16.3238.79
24.4730.22
1.940.65
228.952.12
4.612.39
6.02145.91
9H6W
_64-66239.3
NA
25.8252.36
31.1344.97
1.641.42
188.586.52
5.465.31
5.451705.30
9H6W
_104-106241.0
NA
30.3266.56
24.9358.20
17.185.95
1208.8217.92
24.2010.70
21.181134.92
9H6W
_138-140242.4
NA
25.28132.21
21.0592.46
12.477.66
809.3724.50
22.7417.10
15.52778.23
9H7W
_26-28244.1
NA
29.4295.59
27.1881.78
7.153.47
455.1711.67
12.257.32
8.411036.59
9H7W
_75-77246.2
NA
36.56109.80
36.4098.23
3.832.50
357.639.35
9.637.85
5.80593.51
10H2W
_14-16249.3
NA
0.923.07
4.693.68
7.5713.59
615.2135.60
20.4030.98
16.97728.16
10H2W
_114-116254.5
NA
15.1532.75
18.6233.28
1.091.13
119.112.72
1.652.53
1.23122.52
10H3W
_1-3256.7
NA
29.6338.83
36.7320.52
0.490.58
52.141.87
1.101.97
0.81133.01
10H3W
_44-46259.5
NA
11.8013.32
11.5214.04
0.170.18
20.710.52
0.430.40
0.3029.54
10H3W
_109-111265.1
NA
13.8616.48
17.0916.15
0.220.17
24.690.57
0.630.57
0.2844.74
10H3W
_139-141267.7
NA
15.6021.10
13.4322.07
0.360.37
38.900.96
1.191.02
0.6975.41
10H5W
_9-11277.1
NA
61.37134.51
66.7128.22
5.466.97
425.9717.03
12.8215.32
10.44597.97
10H5W
_49-51279.0
NA
56.01170.93
49.83148.80
7.4413.16
653.1139.68
20.1931.01
17.57927.71
10H5W
_129-131283.0
NA
37.56137.32
40.80111.05
5.489.00
460.7924.08
13.1520.82
11.62954.53
10H6W
_79-81288.6
NA
43.7085.72
54.9413.48
0.611.22
50.602.95
1.852.65
1.67171.44
10H6W
_109-111290.1
NA
8.4317.02
11.052.87
2.353.53
328.558.71
6.499.94
7.3533.81
10H7W
_29-31292.8
NA
58.11151.93
59.9921.12
2.784.47
224.7811.33
6.848.32
5.95343.16
10H7W
_129-131297.7
NA
53.38135.51
49.14126.60
4.997.07
571.5015.46
13.3813.32
11.76748.17
11H1W
_36-38300.7
NA
16.8549.43
13.3242.27
2.003.74
166.209.65
5.087.49
5.03357.66
11H4W
_23-27322.5
NA
52.01203.30
55.65155.57
4.994.72
314.1811.97
8.289.09
7.70447.54
11H5W
_48-50330.9
NA
105.45287.25
112.12238.40
14.932.13
1021.416.78
12.203.11
14.86355.66
Table D3: Mass accumulation rates for measured biomarkers
54
Appendix E Data plotted against depth
Figure E1: Relative abundances of calcareous nannofossil and Pielou's evenness metric (right) vs. depth.
●●
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9590
8580
E. huxleyi + R. parvula
0 40 80
dept
h (m
bsf)
●●
●
●●
●●
●● ●●
●●●● ● ●●●●●●●● ●●
● ●●●● ●● ● ●●● ●● ● ●●●●● ●● ●●●●●●●● ●●●●●● ●●● ●●●●●●●●●●● ●● ●●●●
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small placoliths
●●●
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●●●● ●●● ●●●●●●●● ● ●●●●●●●●●●●●●●●●●●●●●●●●●● ●●●●●● ●●●●●●●●● ●
●●●
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F. profunda
●●
●
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●●
●●●●
●● ●●●● ●● ● ●●● ●● ●
●● ● ● ●● ●●● ●●● ●●● ●●● ●● ●●●●● ●● ●●●●●●●●● ● ●● ●● ●● ●●● ●●●● ●●●●●● ●●
●●●
●●
●
●●●
●
●
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●
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Geophyrocapsaspp.
●●●
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●●●●●●●●●●●●●●●●●●●●●
●●● ●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●
●●●●●
●
●●●●
●
●●
●
●
●
●
●
●
●
●●●
other nannofossils
●●
●
●●
●●
●●●●●● ●●●● ●● ●●●●●●●
●● ● ●● ●● ●●●●●●●● ●●●●● ●●●●●●● ● ●●●●●●●● ●●● ● ●● ●●●●●● ●●●●●●●
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●
●●●
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●
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●
●
●
●
●
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●
●●
●
0.0 0.4 0.8Pielou's
evenness (J)
0 40 80 0 40 80 0 40 80 0 40 80
relative abundance, %
55
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
22 24 26
9590
8580
SST (°C)
dept
h (m
bsf)
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
0 500 1500dinosterol
MAR (ug/cm/kyr)
●
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●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
0 200 400Σ C37
alkenones
●
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●
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●
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●
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●
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●
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●
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●
●
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brassicastanol+stenol
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●
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●
0 10 20β sitosterol
0 20 40
Figure E2: Temperature and biomarker Mass Accumulation Rates plotted against depth. Left: UK'37 Sea Surface Temperature estimates. All others are Mass Accumulation Rates (MAR) of different biomarker classes: Dinosterol is a C30 sterol produced by dinoflagellates. Total C37 alkenones (the sum of the C37:3 and C37:2 alkenones) are produced by Noelaerhabdacean haptophytes. Brassicasterol and brassicastanol, a diagenetic product of the former, are both C28 stenols associated with diatoms, particularly Thalassiosira. β-sitosterol is a C29 sterol produced predominantly by plants.