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The Octave™ Chromatography System is designed for highly productive
puri�cation of chemical and biological compounds. This bench top
8-column system is capable of simulated moving bed chromatography
(SMBC) and other continuous multicolumn protocols that increase
productivity up to 20-fold vs. conventional batch methods. Rather than
�owing �uids sequentially through one static column bed and collecting
fractions as in batch chromatography, SMBC uses a series of interconnected
smaller columns and “moves” the beds in the opposite direction of the �uid
to achieve countercurrent �ow, which greatly facilitates mass transfer and
resolution. Simulated movement of the beds is achieved via multiport valves
that enable periodic switching of the input and output �uids between
adjacent columns in the direction of �uid �ow. All �uid streams are
simultaneously applied and withdrawn at appropriate points between the
columns. When running at a steady state the various stages of separation
are performed simultaneously in di�erent columns in a continuous cycle.
The Octave System overcomes many of the previous limitations of
continuous chromatography, including size and complexity of equipment
and tedious method development. Proprietary valve blocks and control
software automate multicolumn protocols. Intuitive software tools facilitate
conversion of single column data to SMBC parameters. With its small footprint
and modular design, the Octave System brings the high resolution and
e�ciency of SMBC to the bench top, packing more puri�cation capability
into this space than any other similar-size instrument.
• Higher recovery and purity
• Up to 20-fold greater productivity than batch systems
• Up to 90% cost reduction in adsorbents and solvents/bu�ers
• Easy-to-use method development tools and control software
• Robust design with proven performance for many separation chemistries
• Easily customized for speci�c applications
• Available in 3 sizes to obtain grams to kilograms of product per day
ChemistriesA�nity
Chiral
Hydrophobic interaction
Ion exchange
Ion exclusion
Mixed mode
Normal phase
Reversed phase
Size exclusion
TargetsAPIs
Amino acids
Antibiotics
Enantiomers
Fatty acids
Natural products
Oils
Oligosaccharides
Organic acids
Proteins
Sugars
Sugar alcohols
OctaveTM Chromatography SystemContinuous (SMB) chromatography made simple
The power of SMBC on your benchThe power of SMBC on your benchhe power of SMBC on your bench
Modes of operation: Isocratic and StepIsocratic mode is the “classical” SMBC method that utilizes true countercurrent
separation. It is characterized by continuous �ow of a single solvent between
usually 3 or 4 separation zones de�ned by Feed and Desorbent inlets and
Extract (slow moving component) and Ra�nate (fast moving component)
outlets. Isocratic mode is widely used for separating closely related small
molecules such as sugar isomers, enantiomers, and fatty acids.
Step mode is characterized by operation of four or more distinct zones having
di�erent solvent conditions in a bind-elute strategy. Step mode can be used
when the separation factor is large; for example, in a�nity puri�cation of
recombinant proteins and antibodies or fractionating crude preparations by
sequential step elution. A�nity capture e�cency is dramatically enhanced by
having 2 or more columns in the capture zone and loading the �rst column to
near static capacity at high �ow rates; unbound target molecules are captured
by the downstream columns.
Isocratic ModeSeparation of vitamin B12 (1,355 Da; red) and Blue Dextran (2 x 106 Da; blue) by size exclusion on Sephadex® G-25 using eight 5-ml columns in a 3-zone isocratic con�guration on the Octave™ 10 System.
Step ModeProductivities obtained with �ve di�erent Protein A capture resins on the Octave™ 10 System. The 5-step process shown above was used with eight 1-ml columns to purify a human IgG1 mAb from culture �uid at 5 and 7.5 g/L concentrations.
Speci�cations: Octave™ System Dimensions (W / D / H) 21.5 / 21 / 19.5 in (55 / 53 / 50 cm)
Weight 100 lb/45 kg (without pumps)
Power requirements 100–120/220–240 VAC; 2/1 A
Gas inlet supply 330–350 psi (23–24 bar); recommend 99.99% N2
Valve pressure At least 15 psi (1 bar) above �uid pressure
Maximum �uid pressure 270 psi (18.6 bar)
Pumps Four in standard con�guration with maximum of 8; stepper motor dual piston; PEEK™ or stainless steel �ow path; self-�ush pistons; accuracy +/– 3%
Flow rate range 0–12, 0–100, 0–300 ml/min (depending on system)
Valve block 72 pneumatic two-way valves; Ultem® or PCTFE �ow path
Inlets 4 ; each can be shared by 2 pumps
Outlets Maximum 4
Columns Maximum 8
UV-VIS detector Octave™ 4XE UV-VIS Detector available separately; wavelength range 190–840 nm with up to 4 wavelengths measured simultaneously
Ordering Information Maximum Typical OutputCon�guration Cat. No. Pump Speed Column Size Per Day
Octave 10 System H1001K 12 ml/min 1–10 ml 1–30 gOctave 10-HS System H1020K
Octave 100 System H1002K 100 ml/min 10–100 ml Up to 300 gOctave 100-HS System H1014K
Octave 300 II System H1036K 300 ml/min 50–1,000 ml Up to 1 kgOctave 300 II-HS System H1081K
Octave 4XE UV-VIS Detector H1034K
HS systems include PCTFE (rather than Ultem) valve blocks for use with most organic solvents. Users may specify Octave 12-S, 100-S, or 300-S pumps containing stainless steel rather than PEEK (non-metal) �ow paths. Please inquire for custom con�gurations.
Please inquire about Semba’s ProPD™ and ProGMP™ Systems for bioprocess development and single-use manufacturing.
ContactUSA [email protected] +1 866 634 1114
United Kingdom & Republic of Ireland [email protected] +44(0) 7900 300 567
Germany, Austria & Switzerland [email protected] +49 89 66055696
Taiwan & China [email protected] +886-2-85228321 Ext 10
Rest of World [email protected] +1 866 634 1114
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