oie pvs laboratory · june 2017 dr natalie bloch (team leader) dr ibrahim diallo, dr valentyna...
TRANSCRIPT
June
2017
Dr Natalie Bloch (Team Leader)
Dr Ibrahim Diallo, Dr Valentyna Sharandak
PVS Laboratory Mission Report
The Kyrgyz Republic
OIE PVS PATHWAY LABORATORY
REPORT FOR
THE VETERINARY SERVICES OF
KYRGYZ REPUBLIC
(26/06/2017- 08/07/2017)
Dr Natalie Bloch (Team Leader)
Dr Ibrahim Diallo (Technical Expert)
Dr Valentyna Sharandak (Technical Expert)
Disclaimer
This mission has been conducted by an OIE PVS Team authorised by the OIE. However, the views
and the recommendations in this report are not necessarily those of the OIE.
The results of this report remain confidential between the evaluated country and the OIE until such
time as the country agrees to release the report and states the terms of such release.
World Organisation for Animal Health
12, rue de Prony
F-75017 Paris, FRANCE
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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Table of Contents
Table of Contents ................................................................................................................. i
Acronyms and Abbreviations ............................................................................................ iii
Acknowledgements ............................................................................................................. v
Executive Summary ............................................................................................................ 7
I Introduction .................................................................................................................. 9
II Conduct of the OIE PVS Pathway Laboratory Mission ............................................ 11
II.1 Method, objectives and scope of the mission ................................................... 11
II.2 Availability of data relevant to the mission ....................................................... 13
II.3 Context of the mission ........................................................................................ 15
II.3.A General organisation of the national Veterinary Laboratory Network (VLN) .... 15
II.3.B OIE PVS Evaluation for veterinary laboratory Critical Competencies .............. 16
II.3.C Proposals of the OIE PVS Follow-Up Mission for the national Veterinary
Laboratory Network (VLN) ............................................................................................ 18
II.4 Organisation of the mission ............................................................................... 19
III Analysis of the Demand for Veterinary Laboratory Analysis ..................................... 19
III.1 Official Programmes for disease control .............................................................. 19
III.2 Routine diagnostic tests and disease investigation ............................................. 20
III.3 Food safety tests at the national level ................................................................... 21
III.4 Opportunities for demand ...................................................................................... 21
III.4.1 Aquatic diseases .......................................................................................... 22
III.4.2 Honeybee diseases ...................................................................................... 22
III.4.3 Avian diseases ................................................................................................. 22
III.4.4 Diseases of other species ................................................................................ 22
III.5 Estimation of total demand of tests and cost of reagents for the two regions .. 23
IV Analysis of the current resources of the veterinary laboratory network and
budgets .............................................................................................................................. 23
IV.1 Physical resources and budget estimation ....................................................... 24
IV.1.1 Buildings .......................................................................................................... 24
IV.1.2 Information technology ................................................................................... 24
IVI.1.3. Vehicles .......................................................................................................... 24
IV.1.4 Equipment ........................................................................................................ 25
IV.2 Reagents and consumables .................................................................................. 25
IV.3 Human Resources .................................................................................................. 26
IV.4 Estimated budgets for the North and the South regions .................................. 26
V. Sustainability of the budget: estimated cost of tests and proposed tariffs .............. 28
V.1 Estimation of costs of tests .................................................................................... 28
V.2 Estimation of tariffs of tests ................................................................................... 29
VI. Reorganisation of the Kyrgyz veterinary laboratory network and estimation of
number of staff required ................................................................................................... 31
VI.1 Central laboratories - RCVDES .............................................................................. 31
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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VI.2 Regional laboratories ............................................................................................. 32
VI.3 Estimation of number of staff required by the veterinary laboratory network ... 32
VI.3.1 Central laboratories ......................................................................................... 32
VI.3.2 Regional laboratories....................................................................................... 33
VI.4 Training and on-going education .......................................................................... 33
VII Optimisation of the VLN in the next 5 years ............................................................... 35
VII.1 Equipment and sample collection ........................................................................ 35
VII.2 Establish efficient human resources management ............................................. 35
VII.2.1 Establish private laboratories ........................................................................ 35
VII.2.2 Develop awareness programmes................................................................... 35
VII.3 Technical expertise and Quality Assurance ........................................................ 36
VII.4 Establish relevant data management ................................................................... 37
VII.5 Establish a relevant tariff for all tests ................................................................ 37
VII.6 Further reduction the number of regional laboratories ...................................... 37
Conclusions ....................................................................................................................... 41
Annexes............................................................................................................................. 43
Annex 1: Data Recapitulation table for all laboratories of the national network .... 45
Annex 2: Main events and timeline of the mission ......................................................... 47
Annex 3: Analytical Line Tool (equipment required in the regional laboratories) .. 53
Annex 4: Calculation of reagent cost ............................................................................ 55
Annex 5: Demand Tool .................................................................................................... 57
Annex 6: Calculation Tool .............................................................................................. 61
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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Acronyms and Abbreviations
AGID Agarose Gel Immuno Diffusion
Agg Agglutination
AH Animal Health
BBAT Buffered Brucella antigen test
BSL-1, 2, 3 Biological Safety Laboratory Level 1, 2, 3
CC Critical Competency (of the OIE PVS Tool)
CEC Cost Estimation Card (of a CC in the PVS Gap Analysis)
CFT Complement Fixation Test
CIF Cost, Insurance and Freight (Incoterm)
CVO Chief Veterinary Officer
ELISA Enzyme-linked immunosorbent assay
FMD Foot and Mouth Disease
FOB Free On Board (Incoterm)
FS Food Safety
GC Gas Chromatography
GMO Genetically modified organism
IFA Immunofluorescent assay
IT Information Technology
LC-MS Liquid Chromatography Mass Spectrometry
LIMS Laboratory Information Management System
LMPD Livestock and Market Development Project
MAT Microscopic Agglutination Test
NDV Newcastle Disease Virus
NSP Non-structural Protein
OIE World Organisation for Animal Health
PPR Peste des Petits Ruminants
PVS OIE Tool for evaluation of the Performance of Veterinary Services (OIE
PVS Tool)
PCR Polymerase Chain Reaction
qPCR Quantitative Polymerase Chain Reaction
RCVDE Republican Centre for Veterinary Diagnostic and Expertise
RT-PCR Reverse Transcription Polymerase Chain Reaction
SIVPS State Inspectorate for Veterinary and Phytosanitary Security
VLN Veterinary Laboratory Network
VS Veterinary Services
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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Acknowledgements
The OIE PVS Pathway Laboratory Team would like to thank Dr Zhumakanov Kalysbek, Chief
Veterinary Officer, Dr Kasymbekov Joldoshbek, Animal Health Specialist and OIE focal point,
Dr Toroev Mamarasul, Director of the Bishkek Republican Center for Veterinary Diagnostic
and Expertise (RCVDE), Dr Kydyrjychev Asan, Deputy Director of the Bishkek RCVDE, Dr
Abdumomonov Islam, Director of the Osh Republican Center for Veterinary Diagnostic and
Expertise, Dr Alimbekov Shabdan, Chief Specialist and Ms Aalieva Chynara Talantbekovna
– CVO Assistant for their help in the preparation of the mission and also for their continuous
support and patience during the whole course of the mission.
The OIE PVS Pathway Laboratory Team would like to thank all the people from the Bishkek
and the Osh RCVDEs, and all Veterinary Services they met during the mission, for their
welcoming attitude and their professionalism.
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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Executive Summary
This OIE PVS Pathway Laboratory Mission is part of the OIE PVS Pathway and follows the
OIE PVS Evaluation (“diagnosis”) and PVS Gap Analysis (“prescription”) as a part of the
complement of tools that the OIE can provide to support compliance (“treatment”) of the
quality of Veterinary Services (VS) with international standards as defined in the OIE
Terrestrial Code.
The objective of the OIE PVS Pathway Laboratory Mission in the Kyrgyz Republic was to
provide decision makers with information to allocate appropriate resources to the Veterinary
Laboratory Network (VLN) and to make strategic decisions to support accurate and timely
diagnosis, as well as efficient laboratory analysis while ensuring the sustainability of the
laboratory network.
The OIE PVS Pathway Laboratory Team met and discussed with various members of the
State Inspectorate for Veterinary and Phytosanitary Security (SIVPS). The OIE PVS Pathway
Laboratory came to the following conclusions:
The number of clinical samples currently submitted to the entire VLN is low and is
mainly made of brucellosis serology using Buffered Brucella Agglutination Test (BBAT),
microscopic examination of slides for detection of bacteria and blood parasites and
Ascoli test for the detection of Bacillus anthracis in hides. Regional laboratories
perform microscopic examinations, BBAT and preliminary identification of bacteria and
parasites. All other tests are performed exclusively in the two Republican Centre for
Veterinary Diagnostic and Expertise (RCVDE) of Bishkek and Osh .
Now that the Kyrgyz Republic has joined the Eurasian Economic Commission (EEC),
testing for food safety (FS) is going to be mandatory for all food stuff (microbiological
tests, hormones, pesticides and antibiotic residues) and there is also a plan to
undertake a very large scale testing of food stuff for genetically modified organisms
(GMO) and meat origin.
No official data was available for planned FS programmes. Therefore, the OIE PVS
Pathway Laboratory Team used a hypothetical number of tests derived from various
discussions with epidemiology unit staff and members of the SIVPS.
Based on the OIE PVS Pathway Laboratory Team evaluation, both central laboratories
have enough capacity in terms of staff and equipment to process all demand, current
and prospective. Furthermore, it is essential to limit the implementation of complex
analyses such as PCR (Polymerase Chain Reaction) , Enzyme Linked Immunosorbent
Assay (ELISA) and food safety testing in the two central laboratories to ensure that
these tests are performed under controlled and uniform conditions.
Currently, The Kyrgyz Republic benefits from a vast VLN comprising 26 laboratories:
the two RCVDEs in Bishkek and Osh and 24 regional veterinary laboratories. The
regional laboratories will be reduced to a total of 17 in the next few months and both
RCDVEs will have the same status. The analysis presented here has therefore been
based on these 19 laboratories, the 2 central and 17 regional laboratories. The report
analyses the budget of the VLN with 2 central and 17 regional laboratories and offers a
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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number of options to maximise the efficiency of the VLN. In both central laboratories,
the number of sections needs to be reduced, staff need to be relocated and trained to
be multiskilled e.g. most staff should be able to process FS samples.
After assessing the current demand in the Kyrgyz Republic, the OIE PVS Pathway
Laboratory Team also made some recommendations about increasing the demand
for testing in the domain of aquaculture, honey production and poultry production with
a fee recovery scheme.
The Kyrgyz Republic is characterised by a mountainous terrain, which means that
transportation of samples from the field to the laboratories can be lengthy and may
impact on the turn-around time for testing and reporting of results. Therefore,
laboratories must be located strategically to allow a good coverage of the whole
territory. The OIE PVS Pathway Laboratory Team estimates that the number of
regional laboratories can be reduced and still achieve a good coverage of the whole
country in a cost-effective manner.
Consideration should be given to the implementation of private laboratories to perform
tests on clinical cases on a fee for service basis. They will not play any role in the
national programmes which will be the responsibility of the central and regional
laboratories. However, they may play a role in the collection and transport of samples
for further testing. These laboratories could be staffed with former laboratory staff
willing to set up such ventures.
On a more general note, the OIE PVS Pathway Laboratory recommends the following:
The strengthening, and where applicable the finalisation, of a quality management
system across the VLN.
The establishment of relevant data management which will assist in more effective
overall laboratory management, including the analysis of laboratory analysis demand,
the inventory of equipment, the management of human resources and laboratory
analysis related costs.
The establishment of national tariffs for laboratory analysis, using international
reference tariffs as a benchmark, in order to maintain an adequate supply of reagents
and consumables.
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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I Introduction
Following a request to the OIE from the Government of the Kyrgyz Republic, an OIE PVS
Pathway Laboratory Mission was conducted from 26th June to 8th July 2017 by a team of OIE
Experts. This mission was based on the national priorities of the Veterinary Services as
defined in the 2008 OIE PVS Gap Analysis Report and the 2016 OIE PVS Evaluation Follow-
Up mission.
According to the annual reports submitted to the OIE, the following diseases were reported
from 2014 to 2017: rabies, brucellosis (B. abortus and melitensis), anthrax, Echinococus
granulosus and multilocularis, Dourine, Foot and Mouth Disease, Sheep and Goat Pox.
The laboratory Critical Competencies pillar of the 2016 OIE PVS Evaluation Follow-up
Mission recommended as a priority to reorganise sampling methods, kits and transportation
to laboratories; to develop skills of veterinarians for clinical diagnosis and submission of
samples to laboratories for confirmation, develop a laboratory information system (LIMS) and
suggested that food safety should be considered as a main investment for the next decade.
The recent report (2017) on the second stage of the Livestock and Market Development
Project (LMDP) identified Echinococcosis and Brucellosis as priority diseases: and it
provided assistance to prepare the Strategic Plan for Foot and Mouth Disease (FMD) and
Peste des Petits Ruminants (PPR).
These reports do not provide enough detail in the analysis of the National Veterinary Laboratory Network budget.
Given the variety of issues to be addressed, the country requested a specific OIE PVS
Pathway Laboratory Mission. The OIE PVS Pathway Laboratory Mission, for which the
methodology, strategies and recommendations are outlined in this report, builds upon the
2016 OIE PVS Follow-up report, recognizing that laboratories are an integral component of
the Veterinary Services and their priority should meet the needs of the Veterinary Services.
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II Conduct of the OIE PVS Pathway Laboratory Mission
II.1 Method, objectives and scope of the mission
This OIE PVS Pathway Laboratory Mission is part of the OIE PVS Pathway and follows the
OIE PVS Evaluation (“diagnosis”) and PVS Gap Analysis (“prescription”), as a part of the
complement of tools that the OIE can provide to support compliance (“treatment”) of the
quality of VS with international standards as defined in the OIE Terrestrial Code.
Objective:
The purpose of many laboratory-related expert technical evaluation missions is to assess or
evaluate technical capacity and suggest improvements. As these types of missions are
generally constrained by the context and terms of reference, they often do not allow for the
in-depth examination of substantive systemic or strategic issues. The most frequent outcome
of these kinds of missions is at best partial examination of structures and systems, to the
detriment of an overarching or strategic view. Often this leads simply to technical
recommendations and over-investment in structures that are systematically unable to
implement them properly.
The overall objective of the OIE PVS Pathway Laboratory Mission is to provide the decision
makers of Veterinary Services (VS) with information to allocate appropriate resources to the
National Veterinary Laboratory system and to make strategic decisions to support accurate
and timely diagnosis, while ensuring the sustainability of the laboratory system.
Method:
Based on established procedures and practices of the OIE PVS Pathway, an OIE PVS
Pathway Laboratory Mission was undertaken in the Kyrgyz Republic to:
Analyse the demand for veterinary laboratory analysis, mainly through current
laboratory activities, the OIE PVS Gap Analysis and described national
programmes, as well as other partners and/or from prospective demand, including
an overall estimation of the number of samples and tests, of the geographical
distribution of demand, of the cost of consumables (including sampling kits, samples
transport and reagents) and of the international cost;
Analyse the existing or potential service delivery or “supply”, laboratory veterinary
analysis at the national level, mainly delivered by the national veterinary laboratory
infrastructures, as well as by other laboratories (from other public institutions or the
private sector), including their existing capacity and their potential access to new
markets to make the laboratory function a more coherent investment; and
Present a range of sustainable management, organizational, budgetary and
financial solutions, with different options and their costs, in order to assist decision
making at the highest levels related to the cost of autonomy and distribution of the
national laboratory network, including options for externalization through official
delegation to the private sector or to international Reference Laboratories.
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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Outcomes:
The Veterinary Services decision makers of the Kyrgyz Republic should have a better
understanding of:
the laboratory analysis demand in their country (current and prospective);
sustainable ways to organize the national VLN to make the laboratory function a more
coherent investment; and
how to better allocate or advocate for sufficient resources to support accurate and
timely diagnosis of priority animal diseases and veterinary public health issues.
Outputs:
The present report presents the main outputs of the OIE PVS Pathway Laboratory Mission,
which include:
An estimate of the demand for laboratory tests for the next five years, inclusive of the
related costs of consumables
An estimate of the current delivery, or “supply”, of laboratory analysis, including
prospects for potential new markets;
Proposals of sustainable management and organizational solutions of the national
veterinary network; and
Estimates of human, physical and financial resources needed for the implementation of
the best solutions adapted to the veterinary laboratory network.
Tools:
In order to facilitate the analysis of current and prospective data, the OIE PVS Pathway
Laboratory Mission used several tools, from which are extracted the tables presented below
and in annex. Here is a brief description of the tools and the way they were used during the
mission.
1. Demand Tool
The Laboratory Analysis Demand Tool (the first tool used during an OIE PVS Pathway
Laboratory Mission) validates the estimation made during the OIE PVS Gap Analysis Mission
of the budget required for laboratory analyses for consumables in the national laboratory
network (including cost of reagents, sample kits and local shipment). During this portion of
the mission, the current demand, the OIE PVS Gap Analysis demand, the possible
prospective demand for veterinary laboratory analyses as well as the limiting factors and
opportunities for demand are examined with the assistance of the country team.
2. Supply Tool
The purpose of this tool is to mainly collect data of the full array of veterinary laboratory
analyses offered by the laboratory network. This tool establishes automatic ratios for human,
physical and financial resources. It assesses if the laboratory analyses offered by the
laboratory function meet the demand. In the Kyrgyz Republic all data had to be collected
during the mission and the supply tool could not be used.
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3. Calculation Tool
The Calculation Tool is used to simulate different scenarios depending on the distribution of
the budget. It highlights real costs, possible benefits, or required subsidies.
The tables of the first spreadsheet are incorporated directly into Chapter IV of this report.
The second spreadsheet allows the estimation of the “true costs” of laboratory analyses by
taking into account their respective numbers, and the costs of capital, staff and functioning of
the laboratory.
The third spreadsheet allows an estimation and calculation of a national tariff of laboratory
analyses.
4. Analytical Line Tool
The Analytical Line Tool proposes a pre-established check-list of equipment lines, including a
set of equipment and proposed international prices.
II.2 Availability of data relevant to the mission
The Supply Tool was sent to the nominated country authorities for distribution to all
laboratories, to be completed before the mission and returned to the OIE PVS Pathway
Laboratory Mission. The tool was not disseminated so information had to be gathered during
the first week of the mission.
All the laboratory system’s records are kept on monthly paper based records, and sent
quarterly to the Republican Centre for Veterinary Diagnostic and Expertise (RCVDE) by
email.
Data concerning staff, building, equipment and test numbers were extracted from records
provided by the RCVDE in Bishkek. Except for the RCVDE in Bishkek and the RCVDE in
Osh, the value of existing equipment could not be assessed and the OIE PVS team was told
that most of the equipment was obsolete and would be replaced. Given that no data was
provided to the OIE PVS Pathway Laboratory Team before the mission, it was impossible to
use the OIE supply tool, therefore all the required information was entered in a general data
recapitulation table generated by the OIE PVS Pathway Laboratory Team as shown in Annex
1.
The estimated number of animals in the country (Table 1) was provided by the Deputy
Director of the Bishkek RCVDE. Numbers were rounded.
Table 1: Animal Census Estimate (June 2016) from the Statistical Committee of the
Kyrgyz Republic
Cattle Sheep/goats Horses Rabbits Pigs Poultry
TOTAL 1 500 000 6 000 000 467 000 26 000 51 000 5 7 00 000
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The present organizational chart for the State Inspectorate for Veterinary and Phytosanitary
Security (SIVPSS) was supplied during the mission (Figure 1).
Figure 1: Organisational structure of the SIVPSS
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II.3 Context of the mission
II.3.A General organisation of the national Veterinary Laboratory Network (VLN)
The national VLN of the Kyrgyz Republic has been established as a part of the State Inspectorate for Veterinary and Phytosanitary Security (SIVPSS).
The present VLN of the Kyrgyz Republic consists of two central laboratories, the Bishkek Republican Center for Veterinary Diagnostics and Expertise (RCVDE) and the Osh RCVDE, 5 zonal veterinary laboratories and 19 district diagnostic and inter-district laboratories as well as 4 branches laboratories under the control of the district laboratories (see figure 2 below).
Figure 2: Map of the Kyrgyz Republic with the location of the existing laboratories
The 2 Central laboratories are tagged in red
The Zonal laboratories are tagged in purple
The district and inter-district laboratories are tagged in Pink
The branch laboratories are tagged in Yellow
Based on the recommendation of the OIE PVS Gap Analysis Report and the OIE PVS Evaluation Follow-Up Mission, the Kyrgyz State Inspectorate for Veterinary and Phytosanitary Security have reduced the number of laboratories from 26 to 19. This will be implemented in the next 12 months. The reorganisation of the Veterinary Laboratory Network has been approved by the Government of the Kyrgyz Republic Decree № 346 as of 07 June 2017.
There won’t be a distinction any longer between zonal, district, interdistrict and branch laboratories.
Furthermore, for the purpose of the VLN organisation the country has been divided in 2 zones, the North and South regions. There are 9 laboratories in the North region (8 regional laboratories and the Bishkek RCVDE) and 10 laboratories in the South region (9 regional laboratories and the Osh RCVDE). All the regional laboratories located in the
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North region report directly to the RCVDE in Bishkek while all the regional laboratories located in the South region report to the Osh RCVDE. As part of this reorganisation, a new laboratory will be built and equipped in Balykchy in the North. The Talas and Tokmok laboratories in the North and the Batken laboratory in the South will be completely refurbished and equipped, and 7 district and inter-district laboratories will be closed as well as all four branch laboratories. It is worth noting that some of the remaining laboratories are still less than 100 km apart both in the North and the South.
II.3.B OIE PVS Evaluation for veterinary laboratory Critical Competencies
In Table 2, the level of advancement identified by the Veterinary Services through the
2016 OIE PVS Follow-Up mission are indicated in yellow.
Table 2: Critical Competencies (CC) II-1.A & B, II-2 and II-10
II-1 Veterinary laboratory diagnosis
A. Access to veterinary laboratory diagnosis
The authority and capability of the VS to have access to laboratory diagnosis in order to identify and record pathogenic agents, including those relevant for public health, that can adversely affect animals and animal products.
Levels of advancement
PVS 2007 II-1
1
Disease Diagnosis is almost always conducted by clinical means only, with laboratory diagnosis capability hampered by outdated laboratory equipment and techniques.
Gap target 2008 II-1
3
For diseases of zoonotic or economic importance not present in the country, but known to exist in the region or which could enter the country, the VS can collect samples and ship them to a laboratory which results in a correct diagnosis.
PVS 2016
1 Disease diagnosis is almost always conducted by clinical means only, with no access to and use of a laboratory to obtain a correct diagnosis.
2 For major zoonoses and diseases of national economic importance, the VS have access to and use a laboratory to obtain a correct diagnosis.
3 For other zoonoses and diseases present in the country, the VS have access to and use a laboratory to obtain a correct diagnosis.
4
For diseases of zoonotic or economic importance not present in the country, but known to exist in the region and/ or that could enter the country, the VS have access to and use a laboratory to obtain a correct diagnosis.
5
In the case of new and emerging diseases in the region or world, the VS have access to and use a network of national or international reference laboratories (e.g. an OIE Reference Laboratory) to obtain a correct diagnosis.
Although, the OIE PVS has identified some strengths, some weaknesses were also identified:
Lack of organization to ease shipping of samples;
Old methods for sampling kits and transportation with reusable glass tubes, imposing a cost of transportation and manpower which cannot fit with a national programme;
Lack of initiative of private veterinarians to initiate laboratory diagnosis
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II-1 Veterinary laboratory diagnosis
B. Suitability of national laboratory infrastructures
The sustainability, effectiveness and efficiency of the national (public and private) laboratory infrastructures to service the needs of the VS.
Levels of advancement
PVS 2007
-
Gap target 2008
-
PVS 2016
1 The national laboratory infrastructure does not meet the need of the VS.
2
The national laboratory infrastructure meets partially the needs of the VS, but is not entirely sustainable, as organisational deficiencies with regard to the effective and efficient management of resources and infrastructure (including maintenance) are apparent
3
The national laboratory infrastructure generally meets the needs of the VS. Resources and organisation appear to be managed effectively and efficiently, but their regular funding is inadequate to support a sustainable and regularly maintained infrastructure
4
The national laboratory infrastructure generally meets the needs of the VS and is subject to timely maintenance programmes but needs new investments in certain aspects (e.g. accessibility to laboratories, number or type of analyses).
5 The national laboratory infrastructure meets the needs of the VS, and is sustainable and regularly audited.
II-2 Laboratory quality assurance
The quality of laboratories (that conduct diagnostic testing or analysis for chemical residues, antimicrobial residues, toxins, or tests for, biological efficacy, etc.) as measured by the use of formal QA systems including, but not limited to, participation in relevant proficiency testing programmes.
Levels of advancement
PVS 2007
-
Gap target 2008
2 Some laboratories used by the public sector VS are using formal QA systems.
PVS 2016
1 No laboratories used by the public sector VS are using formal QA systems.
2 Some laboratories used by the public sector VS are using formal QA systems.
3 All laboratories used by the public sector VS are using formal QA systems.
4 All the laboratories used by the public sector VS and most or all private laboratories are using formal QA systems.
5
All the laboratories used by the public sector VS and most or all private laboratories are using formal QA programmes that meet OIE, ISO 17025, or equivalent QA standard guidelines.
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II-10 Residue testing
The capability of the VS to undertake residue testing programmes for veterinary medicines (e.g. antimicrobials and hormones), chemicals, pesticides, radionuclides, metals, etc.
Levels of advancement
PVS 2007
-
Gap target 2008
3 A comprehensive residue testing programme is performed for all animal products for export and some for domestic consumption.
PVS 2016
1 No residue testing programme for animal products exists in the country.
2 Some residue testing programme is performed but only for selected animal products for export.
3 A comprehensive residue testing programme is performed for all animal products for export and some for domestic consumption.
4 A comprehensive residue testing programme is performed for all animal products for export and domestic consumption.
5 The residue testing programme is subject to routine quality assurance and regular evaluation.
II.3.C Proposals of the OIE PVS Follow-Up Mission for the national Veterinary Laboratory Network (VLN)
The proposed strategy with regards to the laboratory services was to:
Reorganize sampling methods, transportation and use modern kits and labelling methods of samples for national programmes;
Develop skills of field veterinarians for clinical diagnosis, for necropsy and submission of samples to laboratories for confirmation
Reorganize the laboratory network, possibly with a support of an OIE PVS Pathway Laboratory Mission in order to determine a strategy for sustainable and effective laboratory network;
Ensure capacity of anatomic-pathology and biochemistry in central veterinary laboratories
Implement a computerised laboratory information system and improve the registration of samples in the laboratories using spreadsheet software (e.g. Excel) allowing the analysis of data.
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II.4 Organisation of the mission
During the mission, the OIE PVS Pathway Laboratory Team visited the RCVDE in Bishkek,
the RCVDE in Osh and the Chuiskiy Interdistrict Center for Veterinary Diagnostic and
Expertise
The main events and timeline of the mission are described in Annex 2.
III Analysis of the Demand for Veterinary Laboratory Analysis
As the new organisation of the VLN is taking effect immediately and will be implemented
within the next 12 months, the following analysis and calculations have been made taking
into account the new structure.
III.1 Official Programmes for disease control
The official surveillance programme will involve the analysis of samples for 12 diseases.
Discussion with laboratory staff and the epidemiology department of the State Inspectorate
on Veterinary and Phytosanitary Security under the Government of the Kyrgyz Republic have
indicated that the geographical distribution of animals in the country is such that 65% of all
official programme testing will be performed in Osh region and 35% in Bishkek region with
the exception of Leucosis for which 65% of testing will be done in Bishkek (because of the
high number of breeding and dairy cattle in the region).Table 3 shows the number of samples
required for the official programmes.
Table 3: Official programmes
Disease and technique Total Number Bishkek region Osh region
1. BRUCELLOSIS
Brucellosis by BBAT 1 200 000 420 000
780 000
Brucellosis by CFT
(Complement Fixation Test) (10%
of BBAT)
120 000 42 000 78 000
2. PPR ELISA 60 000 21 000 39 000
3. Echinococcosis
(identification at
the family level –
Taenidae)
20 000 7000 13 000
4. FMD
FMD NSP (Non structural
Protein) ELISA
12 000 4 200 7 800
FMD SP (Structural Protein )
ELISA
8 000 2 800 5 200
5. Anthrax (soil
examination)
1000 350 650
6. Leucosis by AGID 50 000 32 500 17 500
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(Agar Gel Immuno
Diffusion)
7. Sheep and Goat
Pox by AGID
30 000 10 500 19 500
8. Glanders by CFT 28 200 9870 18330
9. Dourine by CFT 28 200 9870 18330
10. Leptospirosis by
MAT (MicroAgglutination
Test) (8 serovars)
40 000 14 000 26 000
11. Avian Influenza by
ELISA
2000 700 1400
12. Rabies by IFA
(immunofluorescence
Assay)
500 175 325
The cost of reagents for these official programmes only is approximately € 185,000 for the
North region and €337,000 for the South. The cost of reagents for these programmes will
have to be budgeted for by the government.
III.2 Routine diagnostic tests and disease investigation
The number of tests performed in each laboratory for clinical samples, as shown in the
Recapitulation table in Annex 1, was supplied by the Bishkek RCVDE. However the data
provided were not consistent as a number of regional laboratories were shown to perform
Ascoli, and/or rabies testing, while discussion with the Chief Veterinary Officer confirmed that
regional laboratories do not perform such tests. Further inconsistencies of number of
performed tests were noted. For instance, since the 1st January 2017 the Bishkek RCVDE
registry had only 776 entries, some of which could be for multiple samples and analyses. The
number of parasitological tests also seemed very high with values of 100,000 tests for the
country whereas examination of registries showed a much lower number of samples.
Overall, the bulk of tests performed are faecal and blood parasitological examinations,
staining and microscopic examination of slides for bacteria, ring test on milk and antrax
testing of hides using Ascoli test. Discussions with the epidemiology team indicated that an
estimated number of 250,000 Ascoli tests will be performed on hides for the whole country.
The number of ring tests performed in 2016 was 25,000 for the whole country. It is estimated
that this number will not change significantly over the next years.
There are currently approximately 5000 tests performed for bee diseases and this number
will probably not change.
The number of other diagnostic tests such as Aujezski, Equine Arthritis, Gumboro, Newcastle
disease, egg drop syndrome etc. is very low and it is estimated that this number will not
change significantly over the next years.
The estimation of the number of tests for the coming years is shown in the Demand tool in
Annex 5.
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
21
All these tests should be performed at customers’ expense and official tariffs should be used
as recommended in chapter V.2
III.3 Food safety tests at the national level
Since 2014 Kyrgyz Republic has been part of the Eurasian Economic Commission (EEC)
which requests from its members a strict control on food stuff. The Kyrgyz Republic is also
preparing a reform of the legislation concerning food safety (FS), which will make testing
compulsory for all exporters of food stuffs as well as all local manufacturers, at the expense
of the businesses. FS testing is currently limited to antibiotic detection in exported food stuffs
using colorimetric detection. The OIE PVS Pathway Laboratory Team was informed that 51
establishments preparing food staff have been approved for the export of food stuff to
member states of the EEC .Therefore member states have to abide to the technical
regulation of the Custom Union ТР ТС 034/2013 on milk and meat products. Testing must be
done for the followings:
Hormones
Heavy Metals (Arsenic, Mercury, Cadmium, Lead etc.)
Pesticides (an average of 6 parameters)
Radiological residues (Cesium and Strontium)
Aflatoxin
It is also anticipated that both central laboratories will be able to provide testing for the
identification of the origin of meat by genotyping and the detection of Genetically Modified
Organisms in food stuffs.
The proposed planning consists in taking 10 samples from each batch of food stuff. Based
on a production of 5 batches per month and per producer, the total yearly sample number for
each producer will be 600 and therefore 30,600 for the whole country for hormone, heavy
metals and pesticides. However no number has been suggested for GMP and genotyping for
meat origin.
Since the repartition of the 51 establishments could not be ascertained, calculations were
made on the basis of equal distribution of the tests between the 2 central laboratories.
This mandatory test will have to be performed at clients’ expenses and the government might
choose to partially subsidize the cost of the tests.
III.4 Opportunities for demand
Aquatic animal diseases, honey bee diseases as well as certain avian and equine diseases
are worth servicing. Most of the diseases of interest can be diagnosed using conventional
methods e.g. microscopy, floatation, bacterial isolation and identification. However, the use
of polymerase chain reaction either conventional or real-time will also ensure an improved
diagnosis of a variety of diseases, and a better diagnostic turn-around time. The 2 central
laboratories are already equipped with real-time PCR machines which are supposed to be
used for food safety testing. It would be more cost efficient to also use real-time PCR for the
diagnosis of some animal diseases. The OIE PVS Pathway Laboratory Team came to the
conclusion that the adaptation of PCR techniques would greatly improve diagnostic outcome
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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at the central level, while the use of the above mentioned conventional techniques would
achieve not only a better diagnostic servicing of common diseases in Kyrgyz Republic, but
also an efficient use of staff at the regional level.
III.4.1 Aquatic diseases
In a mission commissioned by the Ministry of Agriculture and Water Resources and
Production Integrity (MAWRPI) in 2008 ,it was noted that the fisheries production has
declined from more than 1,361 Tons in 1991 to 71 tons in 2006 since the independence in
1991 (Sarieva et al. 2008). The will to develop fisheries production in a bid to alleviate
poverty and ensure food security can be supported by the provision of laboratory diagnostic
services to the fisheries. Some of the most common diseases that affect fish, such as
parasitic and bacterial diseases can be investigated in a bid to help improve the quality of
fish sold in Issyk-Kul lake region and other parts of the country. This seems to be currently
lacking and present an opportunity for developing some fee-for-service agreement with the
MAWRPI.
III.4.2 Honeybee diseases
Although there are national programmes on Varroasis and both the European and American
foulbroods, there is a niche for more fee-for-service testing of local honey production. This
production is already subjected to testing due to food safety requirements. Therefore, it will
be very fitting to accompany that with a sound testing regime of honeybees for the
abovementioned diseases under a fee-for-service regime devised by the SIVPS. More
importantly, the diagnosis of honeybee diseases does not require expensive equipment and
could be performed in all 17 laboratories if required. Such tests consist of microscopy and
mite identification. Other diseases relevant to the context of the Kyrgyz Republic might
considered once the routine diagnosis of varroasis and the 2 common foulbroods have been
implemented.
III.4.3 Avian diseases
According to discussions held with SIVPS staff, there are a number of chicken farms
especially in the Chuy region and also the Talas region. These farms may have private
laboratories that perform basic bacteriological and serological testing of the farm flocks. The
farms will benefit from accessing the expertise of the 2 central laboratories in bacteriology
and virology. The service provided by the two central laboratories would be based on a fee-
for-service, and the farms wouldl send carcasses of dead birds to the central laboratories for
full investigations of the causes of mortality.
III.4.4 Diseases of other species
Currently the Veterinary Research Institute is conducting some research on equine
respiratory viruses such as equine herpesvirus, an important pathogen of horses. It would be
fitting to transfer the technology to the central laboratories once the methodology has been
validated.
Similarly, the diagnosis of echinococcosis is only done to the family level (Taenidae) and no
speciation of the parasite is done. To improve the diagnosis of such an important zoonotic
disease, the Veterinary Research Institute could adopt a published real-time PCR method for
the detection and differentiation of E. multilocularis and E. granulosis and transfer the
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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validated method to the central laboratories.
The Ministry of Agriculture has indicated that they will require to perform some tests to detect
pesticide residues in feed. These tests could be performed by the central laboratories and
charged to the end user.
A number of small private laboratories exist in dairy and chicken farms. The central
laboratory could play a role in offering some internal accreditation scheme and confirmation
tests.
III.5 Estimation of total demand of tests and cost of reagents for the two regions
The total cost of analysis does not include the cost of maintaining laboratory
equipment/infrastructure or the salaries of staff required to perform these tests.
Biochemistry and haematology tests as well as feed content analysis are not taken into
account in the prospective demand tool.
The prospective demand tables for all tests and both region are shown in Annex 5.
Both central laboratories will perform tests for the official surveillance programmes and food
safety (FS).
The regional laboratories will perform the following analysis:
Parasitological examinations, either for clinical samples or for official programmes.
This includes coprology for gastrointestinal parasites e.g. helminths and microscopy
of blood smears for blood parasites e.g. theileria, anaplasma, babesia etc.
Serological tests: Buffered Brucella antigen test (BBAT) and Complement Fixation
test (CFT) as a confirmation test for the detection of Brucellosis in breeding cattle,
male non-vaccinated small ruminants, horses and dogs
Bacteriological examination for clinical samples including staining of slides and
microscopy, initial bacterial culture and gram stain. It is assumed that the regional
laboratories will forward any bacterial isolates to the central laboratory of their region
for further identification.
In the North region, the total number of tests including official programmes is estimated at
approximately 960 000 tests and the cost of reagents at €762 000 (€ 185 000 for the official
programmes only)
In the South region the total number of tests including official programme is estimated at
approximately 1.4 Million tests and the cost of reagents at €932 000 (€337 000 for the official
programmes only).
IV Analysis of the current resources of the veterinary laboratory network and budgets
Considering the new legislation enforcing the existence of two Central Laboratories at the
same level and with independent budgets the analysis has been made for two entities. Since
the number of laboratories will be reduced in the next few months, all calculations were done
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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for the infrastructure of the 2 Central Laboratories and 17 regional laboratories. However, the
total number of staff presently working in the 26 laboratories has been taken into account
since this number will not be reduced in the near future as staff from laboratories to be
closed are due to all be relocated in remaining laboratories.
All data are shown in the recapitulation table in Annex 1.
IV.1 Physical resources and budget estimation
IV.1.1 Buildings
The cost of construction of a building, equivalent to a Biological Safety Laboratory Level 1
(BSL1), is estimated at € 730/m2 in Bishkek and € 350/m2 in regional areas in the North of
the country. In the South, the price is estimated at € 530/m2 in Osh and € 150/m2 in regional
areas.
The cost of construction for a Biological Safety Laboratory Level 2 (BSL-2) according to
international standards (including waste management and air filtering and pressure systems)
is estimated to be double the price of BSL-1. Hence, the calculated cost of €1,500/m2 in
Bishkek for BSL2 and €1,100/m2 in Osh.
OIE estimates the cost of BSL3 to be approximately € 5,000 per square meter (including
waste management, air filtration and pressure systems), regardless of the country.
Maintenance of current buildings is estimated to be five percent of construction costs per
year.
It was estimated that the surface of the new laboratory surface of Balykchy will be
approximately 300 m2.
Data supplied by the Bishkek RCDVE for the surface of each laboratory are shown in the
recapitulation table in Annex I. The surfaces for a number of laboratories could not be
verified but seemed at odd with the other values, therefore surface values were
approximated.
The value of buildings for the 9 laboratories of the North region was estimated at
approximately € 3 Million (see recapitulation table in Annex 1) and the maintenance budget is
estimated at € 156,000. It is noteworthy that the Bishkek RCVDE is planning to upgrade the
250 m2 surface of the virology laboratory into BSL3. If this was the case the total value of the
building would be increased to €4.25 Million and the budget of maintenance would be
€220,000.
The value of buildings for the 10 laboratories of the South region was estimated at
approximately € 783 000 and the maintenance budget is estimated at € 39 000.
IV.1.2 Information technology
All the laboratories have one telephone line and internet connection and computers.
Computers and other office or telecommunication equipment renewal should occur every
three years (33% per year). Local costs are applied.
IVI.1.3. Vehicles
The value of a car has been estimated at €10,000. Renewal of vehicles should occur every
five years (20% per year).
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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IV.1.4 Equipment
The majority of equipment is manufactured in the Russian federation except for the new
Japanese and European equipment for Food Safety analysis recently acquired as part of a
project funded by The Russian Federation and the Republic of Kazakhstan.
A list of equipment in the 26 existing laboratories was supplied during the mission, but the
number of equipment and their total value in all the regional laboratories was inconsistent
with the number of modern equipment required to perform basic bacteriology, parasitology
and serology testing. The existing equipment has no residual value and cannot, in many
instances, be serviced or repaired if they are damaged. Furthermore, the equipment
available in the regional laboratories is obsolete and cannot be reliable to perform to the
standards required for any accredited veterinary laboratory.
For the purpose of budget calculation, international equipment prices were used for the 17
regional laboratories. The value of required equipment was estimated at € 36,600 per
laboratory as shown in Annex 3.
The value of the equipment in the two central laboratories (€3,3 Million for the Bishkek
RCVDE and €1 352,000 for Osh RCDVE was supplied to the OIE Pathway Laboratory Team
by the RCVDE and is based on invoices of the recently acquired equipment.
As a reference and to maintain quality assurance, it is estimated that:
Renewal of laboratory equipment should occur on average every ten years (10% per year)
Calibration and metrology costs are estimated at approximately 10% of the equipment
value per year.
During visits of the Bishkek and Osh RCVDEs the OIE PVS Pathway Laboratory Team
established that both laboratories conducted corrective and preventive maintenance as well
as metrology and calibration programmes. As for other laboratories around the country,
these were reported to be carried out and were mostly coordinated by the Bishkek RCVDE.
IV.2 Reagents and consumables
Sample collection for serum is still not performed with vacutainers but with reusable and
washable glass tubes estimated at 7.5 Som each, which is equivalent to €0,1. Therefore the
cost of sample collection has been adjusted as shown in all the demand tables in Annex 2.
The parametric cost of local transport from the field to the laboratories for 25 samples is
estimated at €11.
The OIE PVS Pathway Laboratory Team was provided with an extensive list of invoices for
various reagents and consumables which allowed the exact calculation of reagent cost
(Annex 4). The data provided were used in the demand table. Because a lot of reagents are
prepared locally, the costs are often at odd with values in other countries.
For example, the cost of MAT consists only of the cost of the Leptospira growth medium,
1 However the Bishkek RCVDE laboratory spends €350 per year to send samples overseas for proficiency testing
while the Osh RCVDE spends €1,000 per year to send samples to Russia. (source of this information is field
interviews)
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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which contains rabbit hyperimmune serum. The cost of maintaining rabbits in the vivarium
was not included, mainly because the estimated costs provided were not realistic e.g. €0,013
to maintain 1 rabbit.
When no invoices were provided, OIE estimation was used, for example for PCR or ELISA
tests.
IV.3 Human Resources
As mentioned above, out of the 26 laboratories throughout the country, 7 will be closed. It is
anticipated that all staff from these 7 laboratories will be relocated in the other 17 remaining
laboratories.
Annex 1 shows that in the North region there are at present a total of 198 staff (including 117
females): 63 staff for Bishkek RCVDE and 135 for the regional laboratories, and in the South
region, there is a total of 153 staff (including 70 females): 26 staff for the Osh RCVDE and
127 for the regional laboratories.
The average monthly net salaries are approximately €221 for a general manager, €164 for a
department or laboratory head, €104 for a specialist (technician) and € 65 for a technical
(support) or office staff.
In terms of per diem, staff receive €6,5 per day when travelling within the country and € 180
when travelling abroad. During discussions at the Bishkek RCVDE, the number of combined
travel days for all staff was estimated at 1,280 per year in the country and 34 days abroad
per year, with a grand total of € 14,440.
In Osh, the total number of days has been estimated at 10 days per year for internal travel,
which works out to be € 65. The cost of international travels in Osh is taken in charge by
international projects.
As a reference and to maintain a reasonable standard, continuing education for staff is
estimated to cost around 5% of the total salary. The estimation of the administration budget
is based on 20% of salary costs.
There is an ongoing programme to train laboratory staff overseas as well as within the
country. There are currently 16 laboratory scientists being trained overseas.
The analysis of the number of staff required to perform all the tests will be done in paragraph
VI.
IV.4 Estimated budgets for the North and the South regions
Since the North and South regions will operate practically independently, separate budgets
were estimated for both.
The budget for each region considers:
The capital investment, which comprises the renewal rate of buildings and premises,
vehicles, information technology (IT), office equipment, telecommunications, laboratory and
other equipment as described in paragraph III.1. The building renewal rate is the main
component of the capital investment.
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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Salaries and remunerations is described in paragraph III.3. The salaries constitute the
smallest component of the budget. This is due to the low salaries paid to the staff and also
the bulk of the staff is made of technical staff and support staff.
- Operating costs e.g. maintenance, calibration and metrology, reagents and
consumables, as well as continuing education and administration expenses etc. are
calculated as a portion of the above data.
The operating costs such as continuing education and administrative expenses are derived
from the salaries and constitutes also a small proportion of the operating costs.
The reagents and consumables costs constitute the main expense of the laboratories
followed by the costs of maintenance, calibration and metrology.
Estimated budgets for both regions are shown in table 4 and 5.
Table 4: Budget for the North region
Capital Investment 566 750 24,67%
Buildings and Premises 3 000 000 1 0,05 150 000 6,53%
Vehicles 10 000 15 0,2 30 000 1,31%
IT and Office Equipment 1 000 75 0,33 24 750 1,08%
Telecommunication Equipment 1 000 10 0,2 2 000 0,09%
Refrigerators & Deep Freezers (-20°C & -80°C) 0,1 - 0,00%
Laboratory Equipment 3 600 000 1 0,1 360 000 15,67%
Other Equipment 0,2 - 0,00%
Salaries and Remuneration 178 760 7,78%
Technical staff 780 40 31 200
Specialists 1 248 58 72 384 3,15%
department heads 1 966 26 51 116 2,23%
directors 2 652 9 23 868
Support Staff 780 29 22 620 0,98%
Per diem and travel allowance in the country 1 440 1 1 440 0,06%
Per diem and travel allowance abroad - 0,00%
Operating Costs 1 551 635 67,55%
Continuing Education (short courses, etc.) Salaries 5% 8 928 0,39%
Administrative Expenditures (office supplies, etc.) Salaries 30% 60 356 2,63%
Reagents and Consumables 762 000 1 762 000 33,17%
Maintenance, Calibration and Metrology Laboratory
Equipment
20%
720 000 31,34%
External Services (Reference Laboratory, External
Analysis, Transport, etc.)
350 100%
350 0,02%
Other - 0,00%
Total 2 297 145 100,00%
Renewal
Rate Annual Budget %
Kyrgyz Republic North Region (Bishkek)9
laboratories Unit Cost Number
If the virology section becomes BSL3 the budget will increase by E45000 as mentioned in
paragraph III.1.1
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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Table 5: Budget for the South region
Capital Investment 246,048
Buildings and Premises 782,950 1 0.05 39,148
Vehicles 10,000 13 0.2 26,000
IT and Office Equipment 1,000 32 0.33 10,560
Telecommunication Equipment 1,000 11 0.2 2,200
Refrigerators & Deep Freezers (-20°C & -80°C) 0.1 -
Laboratory Equipment 1,681,400 1 0.1 168,140
Other Equipment 0.2 -
Salaries and Remuneration 135,417
Technical staff 780 16 12,480
Specialists 1,248 62 77,376
department heads 1,966 16 31,456
directors 2,652 10 26,520
Support Staff 780 18 14,040
Per diem and travel allowance in the country 65 1 65
Per diem and travel allowance abroad -
Operating Costs 1,325,448
Continuing Education (short courses, etc.) Salaries 5% 7,391.60
Administrative Expenditures (office supplies, etc.) Salaries 30% 48,562
Reagents and Consumables 932,215 1 932,215
Maintenance, Calibration and Metrology Laboratory
Equipment
20%
336,280
External Services (Reference Laboratory, External
Analysis, Transport, etc.)
1,000 100%
1,000
Other -
Total 1,706,913
Renewal
Rate Annual Budget
Kyrgyz Republic South Region 10
laboratories Unit Cost Number
It is essential to emphasize on the fact that the figures are estimation to give an indication on
how a budget is calculated. The OIE PVS team has used estimation for basically all data.
V. Sustainability of the budget: estimated cost of tests and proposed tariffs
V.1 Estimation of costs of tests
In any laboratory, the real cost of laboratory analysis depends on relative factors, such as the
number of tests performed by technique and the relative value of each test (which is a
theoretical calculation based on manpower, reagents and the use of equipment for each
test).
In the Calculation Tool, in Annex 6, the “cost of analysis” sheet gives an estimate of the cost
of each test (in Euros (€)), taking into account the number of tests, their relative value (f
column in the Tariff estimation sheet), the standard reagent cost in the country (column o in
the Cost of Analysis sheet) and the proposed annual laboratory budget (excluding reagents
and external services budget lines). The total number of points (value w in the cost of
analysis sheet) is the total number of each tests multiplied by their relative value f).
The relative value of one point is the annual budget excluding reagents and external services
divide by the total number of tests (“estimated cost of analysis” sheet in the calculation tool).
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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The estimated test cost is the sum of the reagent cost, the cost of sampling and local
transport and the relative value f multiplied by the value of one point u.
The lower the value of one point, the higher is the cost of analysis.
The calculation clearly demonstrates that the cost per laboratory analysis, when only a small
number of tests are conducted in each laboratory, is very high.
V.2 Estimation of tariffs of tests
In order to appropriately manage a laboratory, one should be able to determine a tariff based
on the national costs of manpower, equipment and reagents. This enables the laboratory to
propose an appropriate tariff to clients based on its comparative advantages (for instance,
lower cost of manpower) or disadvantages (for instance, higher cost of equipment due to
importation). Finally, this enables the country to analyse the sustainability of the laboratory
network and the possibility to apply partial or total cost recovery methods as compared to
international and national markets.
The calculation tool in Annex 6 estimates a possible national tariff (in Euros (€)) for veterinary
laboratory analyses based on proposed average international tariffs, and the share of costs
between reagents, equipment and staff and is shown on table 6.
Table 6: Tariff estimation.
Type of Analysis
Proposed
Standard
Reagent
Cost
in €
(h)
Proposed
Standard
Share of
Cost:
Reagents
(i)
Proposed
Standard
Share of
Cost:
Equipment
(j)
Proposed
Standard
Share of
Cost:
Staff
(k)
Costs of
Sampling
Kits &
Local
Transport
(l)
Examples
of
Reagent
Costs in
the
country
in €
(n)
Estimated
Reagent
Cost in the
country
in €
(o)
Proposed
Tariff
in €
(p)
Agent Identification
Parasitology 3 0.20 0.10 0.70 0.10 0.00 0.001 1.82
Bacteriology 7.5 0.30 0.20 0.50 0.10 0.00 0.001 5.36
PCR or RTPCR 16 0.40 0.30 0.30 0.10 8.35 20.70
Serology 0.10
ELISA 3 0.50 0.20 0.30 0.10 3.00 3 4.34
CF 1.8 0.30 0.10 0.60 0.10 1.00 0.05 0.82
AGID 1.2 0.30 0.10 0.60 0.10 0.01 0.01 0.56
IFA 3 0.30 0.20 0.50 0.10 0.10 0.1 2.30
BBAT 0.3 0.30 0.10 0.60 0.10 0.02 0.017 0.23
AGGL/ASCOLI 0.6 0.30 0.10 0.60 0.10 0.03 0.03 0.35
MAT 12 0.60 0.20 0.20 0.10 0.01 0.01 4.19
Other Tests 0.10
HPLC/LC-MS 17 0.20 0.60 0.30 0.10 10.00 10 61.63
CPG (GC) 14 0.20 0.60 0.30 0.10 10.00 10 52.54
Spectrophotometry 2.5 0.10 0.50 0.40 0.10 10.00 10 22.81
Food Microbiology Standard 5
Parameters 24 0.40 0.10 0.50 0.10 12.52 9.67
The tariff estimation for FS will have to be reviewed once the country obtains an exact value
for the cost of reagents and decides on a more accurate number of tests. As mentioned
previously the government can choose to partially subsidise the tests or not.
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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There are a number of tests mentioned in the central laboratories which have not been taken
into account: organoleptic analysis, biochemistry analysis: the Qualitative antibiotic detection
should be replaced by modern technology (LC-MS). Biochemistry and organoleptic tests are
not considered by OIE to be part of the services a national laboratory should offer. Such tests
can be performed in local markets by veterinary inspectors with some laboratory skills or by
private laboratories.
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VI. Reorganisation of the Kyrgyz veterinary laboratory network and estimation of number of staff required
VI.1 Central laboratories - RCVDES
The two central laboratories comprise each the following 9 technical departments:
Bacteriology
Virology
Serology
Radiology
Chemical-toxicology
Ascoli
Parasitology
Leptospirosis
Leucosis
The number of departments should be reduced as there is no reason to separate Leucosis
and Ascoli and Leptospirosis from serology. Therefore, Leucosis, Leptospirosis and Ascoli
should be under the Serology department. The bacteriology department will perform all
bacteriological testing of food stuff previously under Food safety. Furthermore, radiology and
chemical-toxicology departments can be combined under the Food Safety department.
In a laboratory processing a high number of samples, there should theoretically be an ELISA
reader and equipment for molecular biology in virology, bacteriology, parasitology and FS.
However, considering the number of samples to be processed, it would be far more efficient
to centralise all ELISA testing in the serology section.
In order to optimize the management system, while avoiding a large number of laboratory
heads and maximizing cost effectiveness, it is suggested to have 5 departments:
Bacteriology performing conventional bacterial identification and isolation,
leptospirosis testing, food bacteriology and also managing all autopsies
Parasitology performing microscopic examination of fecal samples and blood
smears for parasites
Serology performing all ELISA tests (bacterial, virological and
parasitological), brucellosis testing, AGID (e.g. Leucosis) and Ascoli, MAT
(Leptospirosis)
Virology and Molecular Diagnostics performing IFAT (for rabies) and all PCR
and qPCR.
Chemistry for all residues in food stuff.
In order to avoid contamination when performing PCR or qPCR it is essential to separate
“clean” and “dirty” areas. Therefore, there should be 4 or 5 rooms allocated to the different
stages of molecular diagnostic by PCR (sample processing and nucleic acid extraction in a
biosafety cabinet, reagent and master mix preparation in a biosafety cabinet, template
addition to the mastermix in a biosafety cabinet, running of PCR and post-PCR tests). The
OIE PVS Laboratoryy Pathway Team noted that both central laboratories have already set
up adequate PCR facilities and required equipment, including a robot to extract DNA in the
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
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FS laboratory. However, if these facilities were used solely for the purpose of meat origin
identification and GMO detection, they would be vastly underutilised. Given that molecular
techniques can be used for bacterial, viral and parasitological identification, there is no need
in the near future to set up second facilities for that purpose, hence it is suggested that all
PCR testing remain in the existing rooms in the FS department but under the supervision of
the virology and molecular diagnostics head of department.
Although theoretically viruses can be identified by PCR and qPCR techniques, virus isolation
techniques by cell culture may be required if the PCR doesn’t show specific amplification of
target genes. Therefore virus isolation may be required, however this demands specific
technical expertise and considerable financial investment. . Since there is a memorandum of
understanding in place between the State Inspectorate on Veterinary and Phytosanitary
Security, under the Government of the Kyrgyz Republic and the Veterinary Research
Institute, it is strongly suggested to have these tests be performed by the VRI. This would be
more cost-effective than creating costly BSL3 laboratories both from a financial as well as
from an efficiency point of view.
VI.2 Regional laboratories
Tests requiring a low level of technology such as CFT, AGID, and microscopy can be
performed in regional laboratories. However technicians performing these tests should be
properly trained and supervised and there should be a good documented quality control for
all reagents. During discussions with various SIVPS staff there was a mention of some
regional laboratories performing ELISA techniques. This technique requires a high level of
skills and should only be performed by qualified and trained staff using regularly maintained
and calibrated equipment and according to controlled methods. Moreover, it is impossible to
maintain skills and expertise in a variety of techniques if samples are not submitted routinely.
The techniques and equipment used will not be put into practice/use on a basis regular
enough to be sufficient for maintaining proficiency of staff and accuracy of results.
Considering also that the ELISA technology as well as molecular techniques are high
throughput techniques which allow thousands of samples to be processed per day, it is more
efficient to centralise all ELISA testing and molecular techniques in both RCDVEs.
Therefore, it would be more cost-effective if regional laboratories perfom only necropsies,
parasitology examination, BBAT, CFT and microcopic examination of slides for bacteriology
and parasitology. Some regional laboratories might perform a few simple preliminary
bacteriological tests e.g. bacterial isolation before sending either original samples and/or
bacterial isolates to their correponding central laboratories for confirmation and identification
of pathogens.
VI.3 Estimation of number of staff required by the veterinary laboratory network
VI.3.1 Central laboratories
In order to assess the number of staff required to process all tests the OIE PVS Pathway
Laboratory Team referred to a laboratory from an neighbour ex USSR country, performing a
total of 2 Million tests, and approximately the same number of tests for Food Safety as
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
33
suggested in the coming years in the Kyrgyz Republic, with the same equipment. In this
country, there is a total of 85 staff in the central laboratory including directors, head of
departments, technical and support staff. Among these, 12 staff members perform FS
testing.
The total number of staff in the Bishkek RCVDE is currently 63 and in the Osh RCVDE 26. It
is anticipated that the Osh RCDVE will perform more tests than the Bishkek RCVDE.
Therefore, it can be suggested that the total number of staff at the national level in the two
RCDVE is sufficient for the performance of all tests, but it will be necessary to increase the
number of staff in Osh, especially for the FS programme. Based on the present predictions, 6
staff in each central laboratory should be sufficient to perform all FS testing until the time
when the number of tests increases significantly.
In the context of the Kyrgyz Republic, where a lot of livestock move to high pasture from May
to September and the number of samples for serology purpose is considerably reduced
during 5 months of the year, it seems essential that staff is trained to perform several
techniques so they can be re-allocated temporarily to different divisions if and when required.
It is worth considering allocating these staff to FS testing, especially for residue testing of
samples. These staff will not be skilled enough for the handling of LC-MS or GC equipment,
which require considerable expertise, but they can be trained to performed initial processing
of samples for food safety testing.
VI.3.2 Regional laboratories
Discussion with staff in a regional laboratory indicated that one technician is enough to
perform 1,500 BBAT per day, or 250-300 CFT per day or examine 25 microscopic slides per
day for parasitology.The present demand for tests as shown in Annex 1 and the prospective
demand for Official programmes as shown in table 6 show that 4 staff with veterinary training
per regional laboratory are sufficient to perform necropsies, laboratory tasks and
administrative tasks. Taking into account sick leave, maternity leave etc. the number could
be increased to a total of 6 staff per regional laboratory, which will work out to be 102
technicians for the 17 regional laboratories. There should also be 1 director and 1 support
staff in each laboratory making a total of 136 staff. Presently there is a total of 100 staff
(including the directors) in the regional laboratories in the North region and 93 staff (including
the directors ) in the regional laboratories in the South region. There is a need to rationalise
the staff allocation across the Veterinary Laboratory Network, train staff o multitask and avoid
overstaffing.
Detailed staff management is discussed in paragraph VII.2
VI.4 Training and on-going education
Staff in the regional laboratories perform old techniques with poor specificity (such as Serum
agglutination for Brucellosis) and are not regularly trained and audited. It is very important to
establish training programmes at all levels to ensure the reliability and the quality of testing.
The Veterinary Scientific board established by Veterinary Inspectorate could be a useful
resource. Furthermore, the Veterinary board could also be considered in any event where
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
34
some technical problems are encountered e.g. it is unavoidable that scientists in the central
laboratories encounter problems when using molecular techniques.
The Veterinary Research Institute could be a useful resource. Furthermore, the Veterinary
Research Institute could also be considered in any event where some technical problems are
encountered e.g. it is unavoidable that scientists in the central laboratories encounter
problems when using molecular techniques. Once again the Research Institute of Veterinary
should be a useful resource. Similarly, the VLN should consider other training opportunities,
including training overseas, in all disciplines for all staff across the network.
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
35
VII Optimisation of the VLN in the next 5 years
VII.1 Equipment and sample collection
Based on discussions the OIE PVS Pathway Laboratory Team had with various authorities, it
was decided that a stocktake of the equipment located in the laboratories that are due to be
closed should be undertaken. At the same time, an inventory of all the equipment located in
all the laboratories should be also undertaken to assess the equipment needs of all the
laboratories. Once the inventory has been finalised, the equipment should be categorised
into obsolete equipment (need to be disposed of), equipment in working order requiring
either repairs or servicing and equipment in good condition. Further to this assessment, the
relocation of equipment in working order and equipment in good condition should be
considered according to the needs of each laboratory. During this process, each equipment
needs to be individually identified with a tag and a unique number and a list of equipment
must be drawn Furthermore each equipment should have a clear calibration / maintenance
date to meet quality assurance requirements.
The use of glassware for all the tests performed including sampling of blood, petri dishes,
glass pipettes, etc. is highly labour intensive and requires extra staff members who spend
lots of time cleaning and sterilising the glassware. This point has been already mentioned in
other PVS Pathway reports where it was recommended that the laboratories consider
switching to the use of single-use consumables. The OIE PVS Laboratory Pathway Team
supports this recommendation as that would make the laboratory operations more cost
effective.
VII.2 Establish efficient human resources management
VII.2.1 Establish private laboratories
There is an excess of staff in both regions as shown in paragraph VI. The government could
offer incentive for early retirement or financial help to establish private laboratories which
could progressively take over the clinical tests under the supervision of the central
laboratories. Furthermore, staff choosing to establish private laboratories, would be provided
with tailored training on selected techniques in the central laboratories. This training could be
also tailored as a continuing education programme. The private laboratories can also fulfil the
role of sample collection and transportation “agents” for the Veterinary Laboratory Network.
Since a number of laboratories are supposed to close down, the SIVPS should consider the
lease of the buildings of the closed laboratories to staff considering starting up private
laboratories.
VII.2.2 Develop awareness programmes
Regional laboratories staff are mainly busy in the January to May period, when the
surveillance programme for brucellosis generates several thousands of serum samples for
BBAT. During the period of low laboratory testing throughput, laboratory staff can conduct an
outreach awareness campaign for farmers, producers and other potential stakeholders on
disease recognition, efficient use of the laboratory and interpretation of laboratory tests. This
could favour the establishment of good relationships and trust between producers and
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
36
veterinary laboratories and generate higher numbers of samples submitted to the VLN, which
in turn will maintain staff competency.
VII.3 Technical expertise and Quality Assurance
Although OIE usually recommends a strict chain of command from one central laboratory to
regional laboratories, the OIE PVS Pathway Laboratory team acknowledges that in the
context of the Kyrgyz Republic, the existence of two independent central laboratories is
justified. This has been endorsed at the highest government level. Each central laboratory
supervises a number of regional laboratories. However it is imperative to create an
independent technical authority which would supervise both regions. The board of specialists
should include staff from the Veterinary Research Institute and staff with the required
technical expertise in various disciplines e.g. virology, bacteriology, parasitology and
molecular techniques.
Any veterinary laboratory that provides a service is required to have a quality assurance
system in place. The OIE PVS Pathway Laboratory Team noted that the 2 central
laboratories have in place a quality assurance system with written and controlled standard
operating procedures and the use of quality control in various assays. However there is no
quality system in place for media preparation in all sections: for example in bacteriology
where all media are prepared locally, there is no media quality control with reference strains.
Furthermore, the requirements of quality assurance include conducting internal and external
audits on a regular basis, however, the OIE PVS Pathway Laboratory Team noted that there
were no internal and external audit systems.
Regional laboratory staff should be given opportunities to upgrade their skills by
spending some time in the central laboratories, where they will go a training based on
pre-developed training programmes based on national needs as determined by the
central laboratories in conjunction with the Veterinary Research Institute.
The collaboration between the Veterinary Research Institute and the Bishkek RCVDE,
which is based on a Memorandum of Understanding between the 2 institutions, should
be extended to the Osh RCDVE.
Since the control of Echinococcosis is a priority at the national level, both central
laboratories should work with the research institute to adopt and validate a published
PCR technique for the identification of the parasites.
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
37
VII.4 Establish relevant data management
All laboratories have registries for samples received and to record results. At present all data
are sent to the Bishkek RCVDE which do not have a clear and consistent data management
system; therefore their data, mostly paper based, are difficult to analyse and follow in real
time. The VLN should consider transferring their data management system to a computer-
based system, or LIMS, to facilitate data analysis, inform decision making and assist in the
development of rationalised strategies within laboratory resource allocations.
VII.5 Establish a relevant tariff for all tests
It is essential that a laboratory network has one independent budget, even if the government or international organisations are the main source of funding and also the main clients for the official programmes. Both RCDVEs should have a clearly defined budget.
For laboratory activities to be sustainable, the following needs to happen:
All clients must be identified: international projects, government, commercial farms,
smallholders, food processing companies, etc.;
Tariffs must be established for all clients;
Tariff must be documented and adjusted according the client potential income;
Tariffs should be lower for small holders and will need to be sponsored by government subsidies.
The present analysis doesn't take into account biochemistry, organoleptic tests, qualitative antibiotic residue testing which are not the scope of a national laboratory. Such tests should be performed by private laboratories.
VII.6 Further reduction the number of regional laboratories
Once a network of private laboratories is implemented, the government should be able to
consider reducing the number of regional laboratories. Based on geographical constraints,
this number should be between 8 and 10 for the whole country. The cost of buildings and
infrastructure maintenance could be reduced by half, as well as staff number.
If not privatised, some laboratories could be transformed into reception points, primary
processing, storage and delivery of samples of biological material to central or regional
laboratories for testing. Such reception points will require only one staff part-time with
minimal training.
Table 7 and 8 show the budgets for a total of 10 laboratories performing solely tests for the
official programmes, Ascoli and FS with an optimised number of staff. The budget would be
reduced by approximately €200 000 in the South and €300 000 in the North
If the demand increases in the coming years, the cost of reagents will increase but it should
be covered by applying appropriate tariffs.
Although during the Gap Analysis PVS mission, there was a suggestion of reducing the total
number of regional laboratories down to 5, discussions with the CVO during this mission
indicated that this would not happen in the next 5 or 6 years, therefore this option was not
analysed and budgeted for.
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
38
Table 7: Budget for a network of 1 central laboratory and 5 regional laboratories in the
South region
Capital Investment 204,720
Buildings and Premises 591,000 1 0.05 29,550
Vehicles 10,000 7 0.2 14,000
IT and Office Equipment 1,000 19 0.33 6,270
Telecommunication Equipment 1,000 7 0.2 1,400
Refrigerators & Deep Freezers (-20°C & -80°C) 0.1 -
Laboratory Equipment 1,535,000 1 0.1 153,500
Other Equipment 0.2 -
Salaries and Remuneration 88,675
Technical staff 780 14 10,920
Specialists 1,248 50 62,400
department heads 1,966 5 9,830
directors 2,652 6 15,912
Support Staff 780 7 5,460
Per diem and travel allowance in the country 65 1 65
Per diem and travel allowance abroad -
Operating Costs 1,244,310
Continuing Education (short courses, etc.) Salaries 5% 4,953.10
Administrative Expenditures (office supplies, etc.) Salaries 30% 31,357
Reagents and Consumables 900,000 1 900,000
Maintenance, Calibration and Metrology Laboratory
Equipment
20%
307,000
External Services (Reference Laboratory, External
Analysis, Transport, etc.)
1,000 100%
1,000
Other -
Total 1,537,705
Renewal
Rate Annual BudgetKyrgyz Republic Osh Region 5 laboratories Unit Cost Number
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
39
Table 8: Budget for a network of 1 central laboratory and 5 regional laboratories in the
North region
Capital Investment 486,670
Buildings and Premises 2,300,000 1 0.05 115,000
Vehicles 10,000 7 0.2 14,000
IT and Office Equipment 1,000 19 0.33 6,270
Telecommunication Equipment 1,000 7 0.2 1,400
Refrigerators & Deep Freezers (-20°C & -80°C) 0.1 -
Laboratory Equipment 3,500,000 1 0.1 350,000
Other Equipment 0.2 -
Salaries and Remuneration 88,675
Technical staff 780 14 10,920
Specialists 1,248 50 62,400
department heads 1,966 5 9,830
directors 2,652 6 15,912
Support Staff 780 7 5,460
Per diem and travel allowance in the country 65 1 65
Per diem and travel allowance abroad -
Operating Costs 1,437,310
Continuing Education (short courses, etc.) Salaries 5% 4,953.10
Administrative Expenditures (office supplies, etc.) Salaries 30% 31,357
Reagents and Consumables 700,000 1 700,000
Maintenance, Calibration and Metrology Laboratory
Equipment
20%
700,000
External Services (Reference Laboratory, External
Analysis, Transport, etc.)
1,000 100%
1,000
Other -
Total 2,012,655
Renewal
Rate Annual Budget
Kyrgyz Republic Bishkek Region 5 regional
laboratories Unit Cost Number
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
41
Conclusions
The objective of the OIE PVS Pathway Laboratory Mission in the Kyrgyz Republic was to
provide national decision makers with information on how to allocate appropriate resources
to the VLN and to make strategic decisions to support accurate and timely diagnosis.
The OIE PVS Pathway team analysed the sustainability of the national Veterinary Laboratory
Network in the Kyrgyz Republic where there is no private sector to perform laboratory
analysis. Presently, all tests for official veterinary programmes, clinical tests and FS are done
in the state own laboratories.
The opportunities to increase the number of tests and to conduct community outreach in
order to develop the demand for laboratory analysis by livestock small holders were
discussed.
The optimisation of the number of staff, their training and possible relocation were examined
as well as the creation of a network of private laboratories to perform all clinical and
biochemical tests.
In the view of the OIE PVS Pathway Laboratory Team a further reduction in the next coming
years of the number of laboratories would present substantial savings. The redundant
laboratory buildings could be leased to private veterinaries with laboratory expertise (e.g.
former laboratory staff) to develop private laboratories.
It will be essential to implement a thorough quality control system in all laboratories and a
regular ongoing training programme at all levels.
There is a support and understanding from the Veterinary Authority for the improvements
and changes to be brought to the national laboratory network considering the interest they
devoted to the OIE PVS Pathway and the activities initiated by government in view of
improving Veterinary Services.
This PVS Pathway mission should pave the way for a more rapid implementation of different
activities, mentioned above, which are proposed taking into account the activities already
initiated by the Kyrgyz Republic in view of improving their laboratory network.
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
43
Annexes
Annex 1: Data Recapitulation table for all laboratories of
the national network
Annex 2: Main events and timeline of the mission
Annex 3: Analytical Line Tool (equipment required in the
regional labs)
Annex 4: Calculation of reagent cost
Annex 5: Demand Tool
Annex 6: Calculation Tool
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
45
Annex 1: Data Recapitulation table for all laboratories of the national network
Lab 1 Lab 2 Lab 3 Lab 4 Lab 5 Lab 6 Lab 7 Lab 8 Lab 9 Lab 10 Lab 11 Lab 12 Lab 13 Lab 14 Lab 15 Lab 16 Lab 17 Lab 18 Lab 19
Number of Technical staff 27 4 1 1 2 2 1 1 1 1 2 2 2 2 2 2 1 1 1
Number of Specialists 7 13 4 5 8 6 6 5 5 3 7 9 9 6 7 8 4 4 4
Number of department
heads 10 4 1 1 2 2 1 1 1 1 2 3 3 2 3 2 1 1 1
Number of directors 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
Number of technical and
office staff 17 4 1 1 1 3 1 1 2 2 2 2 2 2 1 2 1 1 1
Total number 63 26 8 9 14 14 10 9 10 8 14 17 17 13 14 15 8 8 8
Female 37 13 2 4 9 7 5 4 6 6 10 10 9 10 9 6 5 6
Number IT\phone 2 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
Number of Cars 4 2 1 1 2 1 2 1 1 2 2 2 2 1 1 1 1 1
Number of Computers 51 9 2 3 2 3 1 2 3 3 4 4 3 4 3 3 3 1 3
0
Surface of Buildings in m2 Total Surface in sq m2Average Value in Euros per m2Total value in Euros
surface BSL1 847 1244 750 70 4780 258 1158 234 453 198 330 357 1607 226 1306 700 314 314 15146 500 € 7 573 000 €
surface BSL2 250 250 1 000 € 250 000 €
surface BSL3 250 250 5 000 € 1 250 000 €
Total cost for buildings 9 073 000 €
Value of Equipment in Euros 1 370 000 € 1 352 000 € 25000 23000 28 000 € 58 000 € 20 000 € 25 000 € 37 000 € 41000 37 000 € 46 000 € 40 000 € 34 000 € 13 000 € 45 000 € 54 000 € 44 000 € 54 000 € 3 346 000 €
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
47
Annex 2: Main events and timeline of the mission Date Location/meetings Activities
Monday 26th
June
Bishkek: meetings Meeting with Dr Kalysbek Zhumakanov, CVO, State
Inspectorate on Veterinary and Phytosanitary Safety under the
Government of the Kyrgyz Republic
Meeting with Dr Joldoshbek Kasymbekov, OIE Focal Point for
laboratories, Agricultural Project Implementation Unit
Ms Chynara Aalieva, CVO assistant, State Inspectorate
Dr Alimbekov Shabdan, Chief specialist, Republican centre for
Veterinary Diagnosis and expertise
Dr Ermek Kurmankulov, Head of Department, Republican
Centre for Veterinary Diagnosis and expertise
Dr Shamen Sydykov, Deputy Head, Bishkek city Inspectorate
Dr Zamir, Deputy Head, Alamudin rayon Inspectorate
Tuesday
27th June
Bishkek, State
Inspectorate on
Veterinary and
Phytosanitary
Safety under the
Government of the
Kyrgyz Republic
Bishkek,
Republican centre
for Veterinary
Diagnosis and
Expertise with all
heads of the
departments
Meeting with Dr Kalysbek Zhumakanov, CVO, State
Inspectorate on Veterinary and Phytosanitary Safety under the
Government of the Kyrgyz Republic
Ms Chynara Aalieva, CVO assistant State Inspectorate
Dr Toroev Mamarasul, Head of Republican centre for
Veterinary Diagnosis and Expertise
Dr Kydyrgychev Asan, Deputy Director,
Dr Toimbetov Raxim, Head of the leucosis department
Dr Joldoshbek Kasymbekov, OIE Focal Point for laboratories,
Agricultural project Implementation Unit
Dr Alimbekov Shabdan, Chief specialist
Meeting in the Republican centre for Veterinary Diagnosis and
expertise with all heads of the departments:
Dr Kysaibekov Suiunbubu, head of serological department
Dr Kurmankulov Ermek, head of chemical toxicological
department
Dr Shadyrbekova Dinara, head of radiological department
Dr Musayeva Munara, chief specialist of the virology
department
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
48
Dr Saskarayeva Chynara, head of the department leptospirosis
diagnostic
Dr Sultalomov Zhanbolot, head of the Askoli department
Dr Urusov Kazbych, head of the parasitological department
Wednesday
28th June
Meetings in
Bishkek
Republican centre
for Veterinary
Diagnosis and
Expertise
Dr Kydyrgychev Asan, Deputy Head of Republican centre for
Veterinary Diagnosis and expertise
Dr Toimbetov Rahim, Head of the leucosis department
Dr Alimbekov Shabdan, Chief specialist
Dr Joldoshbek Kasymbekov, OIE Focal Point for laboratories,
Agricultural Project Implementation Unit
Meeting in the Republican centre for Veterinary Diagnosis and
expertise with all heads of the departments
Dr Kysaibekov Suiunbubu, head of serological department
Dr Kurmankulov Ermek, Head of chemical toxicological
department
Dr Shadyrbekova Dynara, head of radiological department
Dr Musayeva Munara, chief specialist of the virology
department
Dr Saskarayeva Chynara, head of the department leptospirosis
diagnostic
Dr Sultanaliev Zhanbolot, head of the Askoli department
Dr Urusov Kazbych, head of the parasitological department
Thursday
29th June
Meetings in
Bishkek
Dr Kadyrgychev Asan, Deputy Head of Republican Centre for
Veterinary Diagnosis and Expertise
Dr Joldoshbek Kasymbekov, OIE Focal Point for laboratories,
Agricultural project Implementation Unit
Dr Toimbetov Rahim, Head of the leucosis department
Dr Kysaibekov Suiunbubu, head of serological department
Dr Kurmankulov Ermek, Head of chemical toxicological
department
Dr Urusov Kazbych, head of the parasitological department
Dr Alimbekov Shabdan, Chief specialist
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
49
Friday 30th
June
Meetings in
Bishkek
State Inspectorate
on Veterinary and
Phytosanitary
Safety under the
Government of the
Kyrgyz Republic
Dr Kalysbek Zhumakanov, CVO, State Inspectorate on
Veterinary and Phytosanitary Safety under the Government of
the Kyrgyz Republic
Dr Joldoshbek Kasymbekov, OIE Focal Point for laboratories,
Agricultural project Implementation Unit
Dr Sotovaldiyev Adylet, inspector
Dr Abdrayev Murat, head of department
Dr Tabaldiyev Tilek, Inspector of the unit of internal supervision
Dr Iskembayeva Gulmairam, Inspector of the unit of internal
supervision
Dr Kuttubek kyzy Gulzada, Inspector of the internal
supervision unit
Saturday 1st
July
Travel to Osh
Meeting in the
Regional Center for
Veterinary
Diagnostic and
Expertise in Osh
Dr Joldoshbek Kasymbekov, OIE Focal Point for laboratories,
Agricultural project Implementation Unit
Dr Abdymomunov Islam, director of Regional Center for
Veterinary Diagnostic and Expertise in Osh
Dr Sulaimanov Astan, head of VS administration of Kara-Suu
district
Dr Pahradinova Akshagul, head of the veterinary and sanitary
expertise and chemical toxicological test
Dr Dolotbayev Nyrgazy, chief specialist
Dr Syrgabayev Danyar, leading specialist,
Dr Bakayeva Aigul, leading specialist
Dr Gaipkulov Abdyrazak, leading specialist
Dr Nabigysan uglu Nyrbek, leading specialist
Dr Primkulova Burul, specialist
Dr Gorelov Viacheslav, head of radiological department
Dr Mamatov Abdybaky, head of bacteriological department
Dr Lubimova Valentyna, chief specialist
Dr Matkasymova Maksuda, technical staff,
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
50
Dr Gaprdjalova Oglay, technical staff
Dr Ashyrbayeva Arzykan, technical staff of parasitology
department
Dr Hasanova Tota, specialist
Dr Caydilda uglu Kalysbek, specialist of serological
department
Dr Ashyaram kyzy Kelsinay
Dr Sotarova A, specialst
Dr Alymdjanova B. Head of virology department
Dr Atokozyeva G. Chief Specialist
Sunday 2nd
July
Meetings in Osh
Dr Joldoshbek Kasymbekov, OIE Focal Point for laboratories,
Agricultural project Implementation Unit
Dr Abdymomunov Islam, director of Regional Center for
Veterinary Diagnostic and Expertise in Osh
Dr Kaarov Emil, head of the VS in the Nookat region
Dr Sulaimanov Astan, head of VS administration of Kara-Suu
district
Dr Kochkorov Zhenysh, veterinarian in the Nookat region
Dr Sabyrov Imaraly, veterinarian in the Nookat region
Dr Ibragymov Zamyrbek, veterinary specialist in the Nookat
region
Monday 3rd
July
Meetings in Osh
Dr Joldoshbek Kasymbekov, OIE Focal Point for laboratories,
Agricultural project Implementation Unit
Dr Abdymomunov Islam, director of Regional Center for
Veterinary Diagnostic and Expertise in Osh
Dr Sulaimanov Arstan, head of VS administration of Karasyisk
district
Travel to Bishkek
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
51
Tuesday 4th July
Meetings in Bishkek Meeting in the Kyrgyz research institute of veterinary after A. Duysheev
Dr Akmatova E., director
Dr Dardykina E., scientific secretary,
Dr Kryzskaya E., virology department
Dr Nurgazyeva A., head of the virology department
Dr Tursunov T., head of the parasitology department
Dr Tolybayeva M., virology department
Dr Chegyrov S., brucellosis department
Dr Isayev M., specialist
OIE PVS Pathway Laboratory brain storming session
Wednesday
5th July
Meetings in
Bishkek
Finalisation of various options
Presentation of preliminary findings and options in a general
meeting
Meeting with Dr Kalysbek Zhumakanov, CVO, State
Inspectorate on Veterinary and Phytosanitary Safety under the
Government of the Kyrgyz Republic
Dr Joldoshbek Kasymbekov, OIE Focal Point for laboratories,
Agricultural project Implementation Unit
Dr Toroev Mamarasul, Head of Republican centre for
Veterinary Diagnosis and Expertise
Thursday
6th July
Meetings in
Bishkek
Report writing
Friday 7th
July
Meetings in
Bishkek and
Tokmok
Meeting in the Chuiskyi Interdistrict Center for Veterinary
Diagnostic and Expertise (in Tokmok)
Dr Baktybek Asanaliyev, director of the center
Dr Dinara Sultanova, head of the bacteriological department
Dr Zhyldyz Zhaparova, specialist parasitology department
Dr Usen Zhusubakunov, head of the serology department
Report writing
Saturday 8th
July
Departure
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
53
Annex 3: Analytical Line Tool (equipment required in the regional laboratories)
Equipment Unit Price in
Euros
Number
of units
Total in
Euros
Microscope 2,000 2 4,000
Incubator 2,000 2 4,000
Autoclave 6,000 1 6,000
Water bath 1,000 2 2,000
Balance 1,000 2 2,000
Distillator 2,000 1 1,000
Fridge 1,000 2 2,000
Freezer 2,000 2 2,000
Centrifuge 4,000 2 8,000
Drying oven 1,500 1 1,500
Shaker 1,500 1 1,500
pH Meter 1,000 1 1,000
Magnetic
stirrer 1,000 2 1,000
Pipettes 200 3 600
Total 36,600
Total for 17
laboratories 622,200
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
55
Annex 4: Calculation of reagent cost
Test Kits/
Reagents
Cost /kit (ml) Note Cost of 1
test
BBAT BBAT
reagent
1300 S/100ml 3300 reactions at 30ul
per reaction
0,30 per dose so 0.005
€ per dose
€0,017
AGID
Kit with Agar
“Difko
Used for leucosis
testing
€0,01
CFT Reagents:
Brucella
Antigen,
positive and
negative
serum,
hemolisinum,
complement
Antigen
392 S/100 ml,
Hemolisinum 4000
doses /3120 S
Positive serum/65
S/1,0 ml
Negative serum\80
S/1,0 ml
Complement
/2000 dose/1400
som
Antigen is used
(working solution)1:75
Complement is used (in
dilution) 1:20
€0,05
Ring Test 20ml for 200 tests=
440
€0,03
AGID (sheep
pox)
Kit with Agar
“Difko”
1 kit =22 000som 1 kit \250 tests €1,13
ELISA TESTS For each of the ELISA kits for which invoices were available the calculation
of the cost was estimated by adding €0.1, which is the cost of 10
micropipette tips per sample.
ELISA PPR 1 kit = 24 000som
1 kit\32 samples
1 kit\88 tests € 3,5
ELISA leucosis 1000\14950 som €0,2
ELISA
Chloramphenic
ol
27550
som\ plate
€3,7
ELISA Aujesky €1,2
ELISA Horse
infection
arthritis
€3,4
ELISA for Foot
and Mouth
Disease (NSP
ELISA)
122000s/5
plates
92 samples/plate
Total
460samples=265so
m/sample
€3,4
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
56
ELISA for Foot
and Mouth
Disease (SP
ELISA)
vaccination
S19700
for 168 samples
117s/sample
Duplicate samples €1.5
Cost of tips 0.89 S
ASCOLI TEST Precipitate
serum
1litre\21000 som =
250 € and allows
10,000 tests,
the cost of each test
is 0,025€
The cost of the acid
phenol solution is
almost negligible and
has been estimated to
0,01€
€0,035
MAT
(leptospirosis)
€0,01
IFA (Rabies)
Immuno
fluorescen
t serum
1 ampul\2100 som Working solution 1:8
0,2 ml per sample
0,03 ml\one test
€0,1
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
57
Annex 5: Demand Tool
North region
Para
sito
logy
Bacte
riolo
gy
PC
R o
r R
TP
CR
OT
HE
R E
LIS
A
ELIS
A F
MD
/SP
ELIS
A F
MD
NS
P
CF
AG
ID L
EU
CO
SIS
AG
ID P
OX
IFA
BB
AT
Pre
cip
itatio
n (
Ascoli)
ELIS
A P
PR
MA
T (
lepto
spirosis
)
Qualit
y c
olo
rim
etr
ic tests
Rin
g T
est
0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1
0,1 1 5 3 1,5 3,4 0,05 0,01 1,3 0,1 0,017 0,035 3,5 0,01 1 0,03 8 11 1 0,01
1 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0
0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1
0,2 1,1 5,1 3,1 1,6 3,5 0,2 0,1 1,4 0,2 0,1 0,1 3,6 0,1 1,1 0,1 8,1 11,1 1,1 0,1
2.1.1. 125000 21 875
2.1.4. 7000 1 680
Foot and mouth disease (NSP 4200
2.1.5. 2800 4 592
2.1.9. 14000 2 100
2.1.13. 175 42
2.2.2. 2065 2 354
2.2.3. 2065 2 3542.2.4. 2065 496
2.3.4. 700 #REF!
2.4.3. 42000 420000 15000 76 470
2.4.11. 32500 4 875
2.5.3. 9870 1 875
2.5.11. 9870 #REF!
2.7.11. 21000 76 440
2.7.14. 10500 #REF!
General parasitology 140000
General Bacteriology 12000
other ELISA 1600
6000 6 840
10000 1 10015300 16 830
2000 15300 126 210
15300 123 930
15300 169 830
7000 35 980
35776 25228 35980 7222 4592 14868 11730,6 4875 15120 42 65940 21875 76440 2100 2280 2550 247860 169830 16830 1100 762 238
149065 22130 7000 2300 2800 4200 61740 32500 10500 175 420000 125000 21000 14000 2000 15000 30600 15300 15300 10000Total Number of Tests (n)
Standard 5 bacteria (TPC, Ecoli, Salm,List, Staph)
Antibiotics
Hormones and anabolics
Pesticides
Cost by Test:
Consumables for National Laboratory n * (a + b + d))
GMO
Radiological tests
HeavyMetals
Glanders
Peste des petits ruminants
Sheep pox and goat pox
Avian influenza
Dourine
Enzootic bovine leukosis
brucellosis all species
Laboratory Tests
(a) Parametric cost (in €) of sampling kits (needle, tubes, etc.)
(b) Parametric cost (in €) of consumables (reagents, kits, etc.)
Leptospirosis
Unit cost of sampling kits and local delivery (a + d)
960 610
Unit cost of laboratory test in country: reagents, sampling
kits and local delivery (a + b + d)
OIE Listed Animal Diseases (as of May 2013)
Food Safety Analysis
Anthrax
Echinococcosis / hydatidosis
Foot and mouth disease ( SP)
Nosemosis of honey bees
American foulbrood of honey bees
European foulbrood of honey bees
Rad
iolo
gic
al T
est
Cost by
Programme:
Consumables
for National
Laboratory
(AB)
Cost by
Programme
Rabies
Other Tests
LC
-MS
(antib
iotiq
ues,h
orm
ones,m
ycoto
xin
e)
GC
-MS
(P
estic
ides,p
estic
ides)
AA
Spectr
ophoto
metr
y
(heavyM
eta
ls)
OIE Animal Health Diagnostic Tests
(as stated in the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals)
Number of Tests
Serology
OIE
Manual C
hapte
r N
um
ber
Agent
Identification
(d) Parametric cost (in €) of local transport for 2 Kg
(25 samples)
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
58
Para
sito
logy
Bacte
riolo
gy
PC
R o
r R
TP
CR
OT
HE
R E
LIS
A
ELIS
A F
MD
/SP
ELIS
A F
MD
NS
P
CF
AG
ID L
EU
CO
SIS
AG
ID P
OX
IFA
BB
AT
Pre
cip
itatio
n (
Ascoli)
ELIS
A P
PR
MA
T (
lepto
spirosis
)
Qualit
y c
olo
rim
etr
ic tests
Rin
g T
est
0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1
0,1 1 5 3 1,5 3,4 0,05 0,01 1,3 0,1 0,017 0,035 3,5 0,01 1 0,03 8 11 1 0,01
1 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0
0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1
0,2 1,1 5,1 3,1 1,6 3,5 0,2 0,1 1,4 0,2 0,1 0,1 3,6 0,1 1,1 0,1 8,1 11,1 1,1 0,1
2.1.1. 125000 21 875
2.1.2. 0
2.1.4. 7000 1 680
Foot and mouth disease (NSP 4200
2.1.5. 2800 4 592
2.1.9. 14000 2 100
2.1.13. 175 42
2.2.2. 2065 2 354
2.2.3. 2065 2 354
2.2.4. 2065 496
2.2.5. 0
2.2.6. 0
2.2.7 0
2.3.2. 0
2.3.4. 700 #REF!
2.3.9. 0
2.3.11. #REF!
2.3.12. #REF!
2.3.14. #REF!
2.4.3. 42000 420000 15000 76 470
2.4.11. 32500 4 875
2.5.3. 9870 1 875
2.5.10. #REF!
2.5.11. 9870 #REF!
2.7.11. 21000 76 440
2.7.14. 10500 #REF!
2.8.8. #REF!
General parasitology 140000
General Bacteriology 12000
other ELISA 1600
6000 6 840
10000 1 100
15300 16 830
0
2000 15300 126 210
15300 123 930
15300 169 830
0
7000 35 980
35776 25228 35980 7222 4592 14868 11730,6 4875 15120 42 65940 21875 76440 2100 2280 2550 247860 169830 16830 1100 762 238
149065 22130 7000 2300 2800 4200 61740 32500 10500 175 420000 125000 21000 14000 2000 15000 30600 15300 15300 10000
Rad
iolo
gic
al T
est
Cost by
Programme:
Consumables
for National
Laboratory
(AB)
Cost by
Programme
Rabies
Other Tests
LC
-MS
(antib
iotiq
ues,h
orm
ones,m
ycoto
xin
e)
GC
-MS
(P
estic
ides,p
estic
ides)
AA
Spectr
ophoto
metr
y
(heavyM
eta
ls)
OIE Animal Health Diagnostic Tests
(as stated in the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals)
Number of Tests
Serology
OIE
Manual C
hapte
r N
um
ber
Agent
Identification
(d) Parametric cost (in €) of local transport for 2 Kg
(25 samples)
960 610
Unit cost of laboratory test in country: reagents, sampling
kits and local delivery (a + b + d)
OIE Listed Animal Diseases (as of May 2013)
Food Safety Analysis
Anthrax
Aujeszky's disease
Echinococcosis / hydatidosis
Foot and mouth disease ( SP)
Avian infectious bronchitis
Nosemosis of honey bees
Small hive beetle infestation (Aethina tumida)
Tropilaelaps infestation of honey bees (Tropilaelaps
Varroosis of honey bees
American foulbrood of honey bees
European foulbrood of honey bees
Infectious bursal disease (Gumboro disease)
Laboratory Tests
(a) Parametric cost (in €) of sampling kits (needle, tubes, etc.)
(b) Parametric cost (in €) of consumables (reagents, kits, etc.)
Leptospirosis
Unit cost of sampling kits and local delivery (a + d)
Newcastle disease
Fowl cholera (pasteurella all species))
Egg Drop Syndrome
Avian influenza
Dourine
Enzootic bovine leukosis
brucellosis all species
Glanders
Peste des petits ruminants
Equine viral arteritis
Sheep pox and goat pox
Swine influenza
Standard 5 bacteria (TPC, Ecoli, Salm,List, Staph)
Antibiotics
Hormones and anabolics
Pesticides
Cost by Test:
Consumables for National Laboratory n * (a + b + d))
Mycology
GMO
Radiological tests
HeavyMetals
Total Number of Tests (n)
P
ara
sito
logy
Bacte
riolo
gy
PC
R o
r R
TP
CR
OT
HE
R E
LIS
A
ELIS
A F
MD
/SP
ELIS
A F
MD
NS
P
CF
AG
ID L
EU
CO
SIS
AG
ID P
OX
IFA
BB
AT
Pre
cip
itatio
n (
Ascoli)
ELIS
A P
PR
MA
T (
lepto
spirosis
)
Qualit
y c
olo
rim
etr
ic tests
Rin
g T
est
0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1
0,1 1 5 3 1,5 3,4 0,05 0,01 1,3 0,1 0,017 0,035 3,5 0,01 1 0,03 8 11 1 0,01
1 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0
0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1
0,2 1,1 5,1 3,1 1,6 3,5 0,2 0,1 1,4 0,2 0,1 0,1 3,6 0,1 1,1 0,1 8,1 11,1 1,1 0,1
2.1.1. 125000 21 875
2.1.2. 0
2.1.4. 7000 1 680
Foot and mouth disease (NSP 4200
2.1.5. 2800 4 592
2.1.9. 14000 2 100
2.1.13. 175 42
2.2.2. 2065 2 354
2.2.3. 2065 2 354
2.2.4. 2065 496
2.2.5. 0
2.2.6. 0
2.2.7 0
2.3.2. 0
2.3.4. 700 #REF!
2.3.9. 0
2.3.11. #REF!
2.3.12. #REF!
2.3.14. #REF!
2.4.3. 42000 420000 15000 76 470
2.4.11. 32500 4 875
2.5.3. 9870 1 875
2.5.10. #REF!
2.5.11. 9870 #REF!
2.7.11. 21000 76 440
2.7.14. 10500 #REF!
2.8.8. #REF!
General parasitology 140000
General Bacteriology 12000
other ELISA 1600
6000 6 840
10000 1 100
15300 16 830
0
2000 15300 126 210
15300 123 930
15300 169 830
0
7000 35 980
35776 25228 35980 7222 4592 14868 11730,6 4875 15120 42 65940 21875 76440 2100 2280 2550 247860 169830 16830 1100 762 238
149065 22130 7000 2300 2800 4200 61740 32500 10500 175 420000 125000 21000 14000 2000 15000 30600 15300 15300 10000
Rad
iolo
gic
al T
est
Cost by
Programme:
Consumables
for National
Laboratory
(AB)
Cost by
Programme
Rabies
Other Tests
LC
-MS
(antib
iotiq
ues,h
orm
ones,m
ycoto
xin
e)
GC
-MS
(P
estic
ides,p
estic
ides)
AA
Spectr
ophoto
metr
y
(heavyM
eta
ls)
OIE Animal Health Diagnostic Tests
(as stated in the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals)
Number of Tests
Serology
OIE
Manual C
hapte
r N
um
ber
Agent
Identification
(d) Parametric cost (in €) of local transport for 2 Kg
(25 samples)
960 610
Unit cost of laboratory test in country: reagents, sampling
kits and local delivery (a + b + d)
OIE Listed Animal Diseases (as of May 2013)
Food Safety Analysis
Anthrax
Aujeszky's disease
Echinococcosis / hydatidosis
Foot and mouth disease ( SP)
Avian infectious bronchitis
Nosemosis of honey bees
Small hive beetle infestation (Aethina tumida)
Tropilaelaps infestation of honey bees (Tropilaelaps
Varroosis of honey bees
American foulbrood of honey bees
European foulbrood of honey bees
Infectious bursal disease (Gumboro disease)
Laboratory Tests
(a) Parametric cost (in €) of sampling kits (needle, tubes, etc.)
(b) Parametric cost (in €) of consumables (reagents, kits, etc.)
Leptospirosis
Unit cost of sampling kits and local delivery (a + d)
Newcastle disease
Fowl cholera (pasteurella all species))
Egg Drop Syndrome
Avian influenza
Dourine
Enzootic bovine leukosis
brucellosis all species
Glanders
Peste des petits ruminants
Equine viral arteritis
Sheep pox and goat pox
Swine influenza
Standard 5 bacteria (TPC, Ecoli, Salm,List, Staph)
Antibiotics
Hormones and anabolics
Pesticides
Cost by Test:
Consumables for National Laboratory n * (a + b + d))
Mycology
GMO
Radiological tests
HeavyMetals
Total Number of Tests (n)
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
59
South region
Para
sito
logy
Bacte
riolo
gy
PC
R o
r R
TP
CR
OT
HE
R E
LIS
A
ELIS
A F
MD
/SP
ELIS
A F
MD
NS
P
CF
AG
ID le
ucosis
AG
ID P
OX
IFA
BB
AT
Pre
cip
itatio
n (
Ascoli)
ELIS
A P
PR
MA
T (
lepto
spirosis
)
Qualit
y c
olo
rim
etr
ic tests
Rin
g T
est
0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1
0,1 1 5 3 1,5 3,4 0,05 0,01 1,13 0,1 0,017 0,035 3,5 0,01 1 0,03 8 11 1 0,01
15 25 40 50 6 6 6 4 10 1 2 6 20 5 1 85 70 25 30
1 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0
350 14,0 14,0 14,0 14,0 14,0 14,0 14,0 14,0 14,0 14,0 14,0 14,0 14,0 14,0 14,0 87,5 87,5 87,5 87,5
0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,0 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1
0,2 1,1 5,1 3,1 1,6 3,5 0,2 0,2 1,3 0,2 0,2 0,2 3,6 0,2 1,1 0,2 8,1 11,1 1,1 0,2
2.1.1. 125000 21 875
2.1.4. 13000 3 120
Foot and mouth disease (NSP 7800
2.1.5. 5200 8 528
2.1.9. 26000 3 900
2.1.13. 325 78
2.2.2. 3835 4 372
2.2.3. 3835 4 372
2.2.4. 3835 920
2.3.4. 700 #REF!
2.4.3. 78000 780000 3265 137 835
2.4.11. 17500 2 625
2.5.3. 18330 3 483
2.5.11. 18330 #REF!
2.7.11. 39000 141 960
2.7.14. 19500 #REF!2.8.8. #REF!
General parasitology 154734
General bacteriology 16361
other ELISA 552
6000 6 840
10000 1 116
15300 16 854
2000 15300 126 234
15300 123 954
15300 169 854
7000 35 980
41177 34235 35980 3931 8528 27612 21785,4 2625 24765 78 122460 21875 141960 3900 2280 555,05 249084 2E+05 17442 1500 932 215
171569 30031 7000 1252 5200 7800 114660 17500 19500 325 780000 125000 39000 26000 2000 3265 30600 15300 15300 10000
Rad
iolo
gic
al T
est
Cost by
Programme:
Consumables
for National
Laboratory
(AB)
Cost by
Programme
Rabies
Other Tests
LC
-MS
(antib
iotiq
ues,h
orm
ones,m
ycoto
xin
e)
GC
-MS
(P
estic
ides,p
estic
ides)
AA
Spectr
ophoto
metr
y
(heavyM
eta
ls)
OIE Animal Health Diagnostic Tests
(as stated in the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals)
Number of Tests
Serology
(e) Parametric cost (in €) of international shipment 2 Kg
(25 samples)
OIE
Manual C
hapte
r N
um
ber
Agent
Identification
(d) Parametric cost (in €) of local transport for 2 Kg
(25 samples)
1 421 302
Unit cost of laboratory test in country: reagents, sampling
kits and local delivery (a + b + d)
OIE Listed Animal Diseases (as of May 2013)
Food Safety Analysis
Anthrax
Echinococcosis / hydatidosis
Foot and mouth disease ( SP)
Nosemosis of honey bees
American foulbrood of honey bees
European foulbrood of honey bees
Laboratory Tests
(a) Parametric cost (in €) of sampling kits (needle, tubes, etc.)
(b) Parametric cost (in €) of consumables (reagents, kits, etc.)
(c) Average price (in €) for international laboratory test
Leptospirosis
Unit cost of sampling kits and local delivery (a + d)
Avian influenza
Dourine
Enzootic bovine leukosis
brucellosis all species
Glanders
Peste des petits ruminants
Sheep pox and goat poxSwine influenza
Standard 5 bacteria (TPC, Ecoli, Salm,List, Staph)
Antibiotics
Hormones and anabolics
Pesticides
Cost by Test:
Consumables for National Laboratory n * (a + b + d))
GMO
Radiological tests
HeavyMetals
Total Number of Tests (n)
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
60
Para
sito
logy
Bacte
riolo
gy
PC
R o
r R
TP
CR
OT
HE
R E
LIS
A
ELIS
A F
MD
/SP
ELIS
A F
MD
NS
P
CF
AG
ID le
ucosis
AG
ID P
OX
IFA
BB
AT
Pre
cip
itatio
n (
Ascoli)
ELIS
A P
PR
MA
T (
lepto
spirosis
)
Qualit
y c
olo
rim
etr
ic tests
Rin
g T
est
0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1
0,1 1 5 3 1,5 3,4 0,05 0,01 1,13 0,1 0,017 0,035 3,5 0,01 1 0,03 8 11 1 0,01
1 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0
0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,0 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1
0,2 1,1 5,1 3,1 1,6 3,5 0,2 0,2 1,3 0,2 0,2 0,2 3,6 0,2 1,1 0,2 8,1 11,1 1,1 0,2
2.1.1. 125000 21 875
2.1.2. 0
2.1.4. 13000 3 120
Foot and mouth disease (NSP 7800
2.1.5. 5200 8 528
2.1.9. 26000 3 900
2.1.13. 325 78
2.2.2. 3835 4 372
2.2.3. 3835 4 372
2.2.4. 3835 920
2.2.5. 0
2.2.6. 0
2.2.7 0
2.3.2. 0
2.3.4. 700 #REF!
2.3.9. 0
2.3.11. #REF!
2.3.12. #REF!
2.3.14. #REF!
2.4.3. 78000 780000 3265 137 835
2.4.11. 17500 2 625
2.5.3. 18330 3 483
2.5.10. #REF!
2.5.11. 18330 #REF!
2.7.11. 39000 141 960
2.7.14. 19500 #REF!
2.8.8. #REF!
General parasitology 154734
General bacteriology 16361
other ELISA 552
6000 6 840
10000 1 116
15300 16 854
0
2000 15300 126 234
15300 123 954
15300 169 854
0
7000 35 980
41177 34235 35980 3931 8528 27612 21785,4 2625 24765 78 122460 21875 141960 3900 2280 555,05 249084 2E+05 17442 1500 932 215
171569 30031 7000 1252 5200 7800 114660 17500 19500 325 780000 125000 39000 26000 2000 3265 30600 15300 15300 10000Total Number of Tests (n)
Standard 5 bacteria (TPC, Ecoli, Salm,List, Staph)
Antibiotics
Hormones and anabolics
Pesticides
Cost by Test:
Consumables for National Laboratory n * (a + b + d))
Mycology
GMO
Radiological tests
HeavyMetals
Glanders
Peste des petits ruminants
Equine viral arteritis
Sheep pox and goat pox
Swine influenza
Newcastle disease
Fowl cholera (pasteurella all species))
Egg Drop Syndrome
Avian influenza
Dourine
Enzootic bovine leukosis
brucellosis all species
Laboratory Tests
(a) Parametric cost (in €) of sampling kits (needle, tubes, etc.)
(b) Parametric cost (in €) of consumables (reagents, kits, etc.)
Leptospirosis
Unit cost of sampling kits and local delivery (a + d)
1 421 302
Unit cost of laboratory test in country: reagents, sampling
kits and local delivery (a + b + d)
OIE Listed Animal Diseases (as of May 2013)
Food Safety Analysis
Anthrax
Aujeszky's disease
Echinococcosis / hydatidosis
Foot and mouth disease ( SP)
Avian infectious bronchitis
Nosemosis of honey bees
Small hive beetle infestation (Aethina tumida)
Tropilaelaps infestation of honey bees (Tropilaelaps
Varroosis of honey bees
American foulbrood of honey bees
European foulbrood of honey bees
Infectious bursal disease (Gumboro disease)
Rad
iolo
gic
al T
est
Cost by
Programme:
Consumables
for National
Laboratory
(AB)
Cost by
Programme
Rabies
Other Tests
LC
-MS
(antib
iotiq
ues,h
orm
ones,m
ycoto
xin
e)
GC
-MS
(P
estic
ides,p
estic
ides)
AA
Spectr
ophoto
metr
y
(heavyM
eta
ls)
OIE Animal Health Diagnostic Tests
(as stated in the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals)
Number of Tests
Serology
OIE
Manual C
hapte
r N
um
ber
Agent
Identification
(d) Parametric cost (in €) of local transport for 2 Kg
(25 samples)
P
ara
sitolo
gy
Bacte
riolo
gy
PC
R o
r R
TP
CR
OT
HE
R E
LIS
A
ELIS
A F
MD
/SP
ELIS
A F
MD
NS
P
CF
AG
ID leucosis
AG
ID P
OX
IFA
BB
AT
Pre
cip
itation (
Ascoli)
ELIS
A P
PR
MA
T (
lepto
spirosis
)
Qualit
y c
olo
rim
etr
ic tests
Rin
g T
est
0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1
0,1 1 5 3 1,5 3,4 0,05 0,01 1,13 0,1 0,017 0,035 3,5 0,01 1 0,03 8 11 1 0,01
1 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0
0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,0 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1
0,2 1,1 5,1 3,1 1,6 3,5 0,2 0,2 1,3 0,2 0,2 0,2 3,6 0,2 1,1 0,2 8,1 11,1 1,1 0,2
2.1.1. 125000 21 875
2.1.2. 0
2.1.4. 13000 3 120
Foot and mouth disease (NSP 7800
2.1.5. 5200 8 528
2.1.9. 26000 3 900
2.1.13. 325 78
2.2.2. 3835 4 372
2.2.3. 3835 4 372
2.2.4. 3835 920
2.2.5. 0
2.2.6. 0
2.2.7 0
2.3.2. 0
2.3.4. 700 #REF!
2.3.9. 0
2.3.11. #REF!
2.3.12. #REF!
2.3.14. #REF!
2.4.3. 78000 780000 3265 137 835
2.4.11. 17500 2 625
2.5.3. 18330 3 483
2.5.10. #REF!
2.5.11. 18330 #REF!
2.7.11. 39000 141 960
2.7.14. 19500 #REF!
2.8.8. #REF!
General parasitology 154734
General bacteriology 16361
other ELISA 552
6000 6 840
10000 1 116
15300 16 854
0
2000 15300 126 234
15300 123 954
15300 169 854
0
7000 35 980
41177 34235 35980 3931 8528 27612 21785,4 2625 24765 78 122460 21875 141960 3900 2280 555,05 249084 2E+05 17442 1500 932 215
171569 30031 7000 1252 5200 7800 114660 17500 19500 325 780000 125000 39000 26000 2000 3265 30600 15300 15300 10000Total Number of Tests (n)
Standard 5 bacteria (TPC, Ecoli, Salm,List, Staph)
Antibiotics
Hormones and anabolics
Pesticides
Cost by Test:
Consumables for National Laboratory n * (a + b + d))
Mycology
GMO
Radiological tests
HeavyMetals
Glanders
Peste des petits ruminants
Equine viral arteritis
Sheep pox and goat pox
Swine influenza
Newcastle disease
Fowl cholera (pasteurella all species))
Egg Drop Syndrome
Avian influenza
Dourine
Enzootic bovine leukosis
brucellosis all species
Laboratory Tests
(a) Parametric cost (in €) of sampling kits (needle, tubes, etc.)
(b) Parametric cost (in €) of consumables (reagents, kits, etc.)
Leptospirosis
Unit cost of sampling kits and local delivery (a + d)
1 421 302
Unit cost of laboratory test in country: reagents, sampling
kits and local delivery (a + b + d)
OIE Listed Animal Diseases (as of May 2013)
Food Safety Analysis
Anthrax
Aujeszky's disease
Echinococcosis / hydatidosis
Foot and mouth disease ( SP)
Avian infectious bronchitis
Nosemosis of honey bees
Small hive beetle infestation (Aethina tumida)
Tropilaelaps infestation of honey bees (Tropilaelaps
Varroosis of honey bees
American foulbrood of honey bees
European foulbrood of honey bees
Infectious bursal disease (Gumboro disease)
Rad
iolo
gic
al T
est
Cost by
Programme:
Consumables
for National
Laboratory
(AB)
Cost by
Programme
Rabies
Other Tests
LC
-MS
(antibio
tiques,h
orm
ones,m
ycoto
xin
e)
GC
-MS
(P
esticid
es,p
esticid
es)
AA
Spectr
ophoto
metr
y
(heavyM
eta
ls)
OIE Animal Health Diagnostic Tests
(as stated in the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals)
Number of Tests
Serology
OIE
Manual C
hapte
r N
um
ber
Agent
Identification
(d) Parametric cost (in €) of local transport for 2 Kg
(25 samples)
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
61
Annex 6: Calculation Tool North region
Capital Investment 566 750 24,67%
Buildings and Premises 3 000 000 1 0,05 150 000 6,53%
Vehicles 10 000 15 0,2 30 000 1,31%
IT and Office Equipment 1 000 75 0,33 24 750 1,08%
Telecommunication Equipment 1 000 10 0,2 2 000 0,09%
Refrigerators & Deep Freezers (-20°C & -80°C) 0,1 - 0,00%
Laboratory Equipment 3 600 000 1 0,1 360 000 15,67%
Other Equipment 0,2 - 0,00%
Salaries and Remuneration 178 760 7,78%
Technical staff 780 40 31 200
Specialists 1 248 58 72 384 3,15%
department heads 1 966 26 51 116 2,23%
directors 2 652 9 23 868
Support Staff 780 29 22 620 0,98%
Per diem and travel allowance in the country 1 440 1 1 440 0,06%
Per diem and travel allowance abroad - 0,00%
Operating Costs 1 551 635 67,55%
Continuing Education (short courses, etc.) Salaries 5% 8 928 0,39%
Administrative Expenditures (office supplies, etc.) Salaries 30% 60 356 2,63%
Reagents and Consumables 762 000 1 762 000 33,17%
Maintenance, Calibration and Metrology Laboratory
Equipment
20%
720 000 31,34%
External Services (Reference Laboratory, External
Analysis, Transport, etc.)
350 100%
350 0,02%
Other - 0,00%
Total 2 297 145 100,00%
Renewal
Rate Annual Budget %
Kyrgyz Republic North Region (Bishkek)9
laboratories Unit Cost Number
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
62
566 750
178 760
789 285
1 534 795
0,44
Type of Analysis
Proposed
Standard
Relative
Value
in points
(f)
Estimated
Reagent
Cost in the
country
in €
(o)
Cost of
Sampling
Kits &
Local
Transport
in €
(l)
Total
Estimated
Reference
International
Price
in €
(m)
Number
of
Tests
(v)
Number
of
Points
(w) = v * f
Estimated
Test Cost
in €
(x) = o + l
+ (f * u)
Possible
Income
Generated
under Full
Cost
Recovery
(y) = v * x
% of
Economic
Activity
J
% of
Volume of
Activity
K
Agent Identification
Parasitology 7,5 0,001 0,10 15,10 150 000 1 125 000 3,39 509 073 23,56% 32,18%
Bacteriology 12,5 0,001 0,10 25,10 22 000 275 000 5,59 122 959 5,69% 7,87%
PCR or RTPCR 20 5 0,10 40,10 700 14 000 13,88 9 717 0,45% 0,40%
Serology
ELISA 3 3 0,10 6,10 30 000 90 000 4,42 132 514 6,13% 2,57%
CF 3 0,05 0,10 6,10 61 800 185 400 1,47 90 669 4,20% 5,30%
AGID 2 0,01 0,10 4,10 43 000 86 000 0,99 42 488 1,97% 2,46%
IFA 5 0,1 0,10 10,10 175 875 2,40 419 0,02% 0,03%
BBAT 0,5 0,017 0,10 1,10 435 000 217 500 0,34 146 387 6,78% 6,22%
Agg (ASCOLI) 1 0,03 0,10 2,10 125 000 125 000 0,57 71 130 3,29% 3,58%
PRN/CAT 1 0,28 0,10 2,10 0 0,82 - 0,00% 0,00%
HI 3 0,83 0,10 6,10 0 2,25 - 0,00% 0,00%
MAT 10 0,01 0,10 20,10 0 4,50 - 0,00% 0,00%
Other Tests
LC-MS 42,5 7,85 0,10 85,10 15 300 650 250 26,61 407 123 18,84% 18,60%
CPG 35 10 0,10 70,10 15 300 535 500 25,47 389 637 18,03% 15,32%
Spectrophotometry 12,5 10 0,10 25,10 15 300 191 250 15,59 238 497 11,04% 5,47%
Food Microbiology Standard 5
Parameters 15 11,08 0,10 30,100 17,77 - 0,00% 0,00%
Totals 913 575 3 495 775 2 160 612 100,00% 100,00%
3. Estimated Cost of Veterinary Laboratory Analysis
(u) = H6 / G36 Value of 1 Point(Excluding Reagents)
Annual Budget (Excluding Reagents & External Services)
Other Costs (Excluding Reagents & External Services)
Staff
Capital Investment
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
63
South region
Capital Investment 247 908 14,48%
Buildings and Premises 782 950 1 0,05 39 148 2,29%
Vehicles 10 000 13 0,2 26 000 1,52%
IT and Office Equipment 1 000 32 0,33 10 560 0,62%
Telecommunication Equipment 1 000 11 0,2 2 200 0,13%
Refrigerators & Deep Freezers (-20°C & -80°C) 0,1 - 0,00%
Laboratory Equipment 1 700 000 1 0,1 170 000 9,93%
Other Equipment 0,2 - 0,00%
Salaries and Remuneration 135 417 7,91%
Technical staff 780 16 12 480
Specialists 1 248 62 77 376 4,52%
department heads 1 966 16 31 456 1,84%
directors 2 652 10 26 520 1,55%
Support Staff 780 18 14 040 0,82%
Per diem and travel allowance in the country 65 1 65 0,004%
Per diem and travel allowance abroad - 0,00%
Operating Costs 1 329 168 77,62%
Continuing Education (short courses, etc.) Salaries 5% 7 391,60 0,43%
Administrative Expenditures (office supplies, etc.) Salaries 30% 48 562 2,84%
Reagents and Consumables 932 215 1 932 215 54,44%
Maintenance, Calibration and Metrology Laboratory
Equipment
20%
340 000 19,85%
External Services (Reference Laboratory, External
Analysis, Transport, etc.)
1 000 100%
1 000 0,06%
Other - 0,00%
Total 1 712 493 100,00%
Renewal
Rate Annual Budget %
Kyrgyz Republic South Region(Osh) 10
laboratories Unit Cost Number
Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017
64
247 908
135 417
395 953
779 278
0,14
Type of Analysis
Proposed
Standard
Relative
Value
in points
(f)
Estimated
Reagent
Cost in the
country
in €
(o)
Cost of
Sampling
Kits &
Local
Transport
in €
(l)
Total
Estimated
Reference
International
Price
in €
(m)
Number
of
Tests
(v)
Number
of
Points
(w) = v * f
Estimated
Test Cost
in €
(x) = o + l
+ (f * u)
Possible
Income
Generated
under Full
Cost
Recovery
(y) = v * x
% of
Economic
Activity
J
% of
Volume of
Activity
K
Agent Identification
Parasitology 7,5 0,001 0,10 15,10 188 000 1 410 000 1,13 211 666 10,43% 24,73%
Bacteriology 12,5 0,001 0,10 25,10 13 700 171 250 1,81 24 785 1,22% 3,00%
PCR or RTPCR 20 8,35 0,10 40,10 700 14 000 11,18 7 828 0,39% 0,25%
Serology
ELISA 3 3 0,10 6,10 53 000 159 000 3,51 186 028 9,17% 2,79%
CF 3 0,05 0,10 6,10 114 600 343 800 0,56 64 171 3,16% 6,03%
AGID 2 0,01 0,10 4,10 37 000 74 000 0,38 14 182 0,70% 1,30%
IFA 5 0,1 0,10 10,10 325 1 625 0,88 287 0,01% 0,03%
BBAT 0,5 0,017 0,10 1,10 780 000 390 000 0,19 144 554 7,13% 6,84%
Agg (ascoli) 1 0,03 0,10 2,10 125 000 125 000 0,27 33 331 1,64% 2,19%
MAT 10 0,01 0,10 20,10 26 000 260 000 1,48 38 389 1,89% 4,56%
Other Tests
HPLC 42,5 10 0,10 85,10 30 600 1 300 500 15,91 486 775 23,99% 22,81%
CPG 35 10 0,10 70,10 30 600 1 071 000 14,88 455 413 22,45% 18,78%
Spectrophotometry 12,5 10 0,10 25,10 30 600 382 500 11,81 361 329 17,81% 6,71%
Food Microbiology Standard 5
Parameters 15 12,52 0,10 30,100 14,67 - 0,00% 0,00%
Totals 1 430 125 5 702 675 2 028 739 100,00% 100,00%
3. Estimated Cost of Veterinary Laboratory Analysis
(u) = H6 / G36 Value of 1 Point(Excluding Reagents)
Annual Budget (Excluding Reagents & External Services)
Other Costs (Excluding Reagents & External Services)
Staff
Capital Investment