okfkzd izfrosnu - circ.org.in · okf"kzd izfrosnu annual report 2014-15 hkkÑvuqi&dsunzh;...

okf"kZd izfrosnuANNUAL REPORT

2014-15

HkkÑvuqi&dsUnzh; Xkksoa’k vuqla/kku laLFkku¼Hkkjrh; Ñf"k vuqla/kku ifj"kn~½

xzkl QkeZ jksM] iksLV ckWDl la-17] esjB Nkouh 250 001 ¼m-iz-½] Hkkjr

ICAR-Central Institute for Research on Cattle(Indian Council of Agricultural Research)

Grass Farm Road, Post Box No. 17, Meerut Cantt.- 250 001 (U.P.), India

Indian Council of Agricultural Research

ICAR-Central Institute for Research on Cattle Annual Report 2014-15

ii

Annual Report 2014-15

ICAR-Central Institute for Research on Cattle(Indian Council of Agricultural Research)Grass Farm Road, Post Box No. 17,Meerut Cantt.- 250 001 (U.P.), IndiaPhone: 0121-2657136 (Director); 0121- 2645598, 2656021 (EPABX)Fax: 0121-2657134; Email: [email protected], [email protected] site: http://www.circ.org.in

Published by : Dr. B. Prakash, Director

Editors : Dr. Shrikant Tyagi, In-charge, Priority setting,Monitoring & EvaluationDr. T.V. Raja, Senior Scientist

Hindi Translation & Editing : Dr. Sanjeev Kumar Verma, Senior Scientist

Computer Assistance : Dr. Puneet Kumar, Research Associate

Stenographic Assistance : Mr. Pankaj Kumar Gautam, Assistant

Publication Committee : Dr. A.K. Das, Principal ScientistDr. D.K. Mandal, Senior ScientistDr. Sanjeev Kumar Verma, Senior ScientistDr. Dr. A.V.S. Sirohi, Senior ScientistDr. Yogesh Kumar Soni, Scientist

Correct citation : Annual Report 2014 – 15ICAR-Central Institute for Research on Cattle,Meerut Cantt. – 250 001

Printed at : Yugantar Prakashan Pvt. Ltd.WH-23 Mayapuri Industrial Area, Phase-I,New Delhi-110064Ph.: 011-28115949, 28116018 (O)09811349619, 9953134595 (M)

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Annual Report 2014-15 ICAR-Central Institute for Research on Cattle


CONTENTS

Sl.No. Subject Page No.

1. Preface v

2. CIRC: An Introduction 13. dk;Zdkjh lkjka’k@Executive Summary 44. Organogram 16

5. Staff position, Financial statement, Resource generation 17

6. Research achievements 19

(I) AICRP on Cattle 19(A) Development of crossbred strain of cattle: Frieswal Project 19(B) Genetic improvement of crossbred cattle under field 24 conditions: FPT(C) Conservation and genetic improvement of indigenous cattle 37 breeds: IBP

(II) Institutional Programmes 44(A) Molecular characterization 44(B) Semen production and preservation 47(C) Augmentation of reproductive efficiency 51(D) Improvement of cattle through nutritional manipulation 51

(III) Externally Funded Project 53(IV) Extension Activities 55(V) Technology/Concepts 59

7. Research projects 60

8. Publications 63

9. Training/Conference/Seminar/Workshops attended 75

10. Seminar organized/Trainings imparted 78

11. Professional recognitions 79

12. Institute Technology Management Unit and RTI 79

13. Important committees (RAC/IMC/IJSC) and meetings (Review Meet/RAC/ 80Hindi Workshop)

14. Monthly Seminars 86

15. Important Institute activities (Days celebrated/Sports etc.) 86

16. Distinguished visitors 94

17. Staff position 96

18. Personnel milestones (Promotions /Joining/Transfers) 98

19. Standing Institutional Committees 99

20. Results-Framework Document (RFD) 104

v



PREFACE

The livestock sector of India is one of the largest in the world and has played a pivotal rolein the national economy and socio economic development of the country. Even though livestocksector is considered as a sub sector of agriculture, under Indian context both are intrinsicallylinked with each other as the livestock is raised essentially on crop residue or agricultural by-products while agricultural demands of draught power and manure are met by livestock origin.The contribution of livestock sector to the National economy in terms of Gross DomesticProduct (GDP) during the year 2012-13 was 4.11 per cent at current price and 3.46 per centat constant price, while its contribution to the whole agricultural sector was 27.25 and 29.20per cent, respectively (BAH&FS, GOI, AHS-15, 2014). It is also an accepted fact that amongvarious livestock enterprises, dairying is rated as the most important as it grows rapidly thanany other agriculture or livestock sectors and finds acceptance among all sectariancommunities of the country.

For over a decade India has been the largest milk producer in the world and in 2013-14the total national milk production was estimated as 137.70 MT accounting for 17 per cent ofthe total world production. The milk production in the country is primarily contributed bybuffaloes (51%) and closely followed by cattle (45%). The national average annual growthrate of milk is 3.54 per cent against the world average of 2.2 per cent which shows thesustained growth in availability of milk and milk products for the growing population. The percapita availability of milk is 290 g/ day which is slightly higher than the world average (285 g/day).

The cattle genetic resources of India is very rich and diverse with 39 well defined registeredbreeds classified based on their utility as dairy (4), dual (8) and draught (27). India is theharbinger of largest livestock population and according to the 19th Livestock Census ofGovernment of India the cattle population of the country in the year 2012 was 190.90 millionwhich constituted 15.06 per cent of the total world population. The various livestockdevelopmental programmes implemented by the government since independence haveresulted in increasing the milk production of the country from 17.00 (1950-51) to 137.70million tonnes (2013-14). Crossbreeding of indigenous cattle with high productive temperatedairy breeds has played a significant role in increasing the milk production during the last fourdecades.

In spite of all the positive facts, India has the daunting challenge to meet the future milk/dairy products demand of the ever increasing population of the country. The consumer demandfor milk and milk products is increasing at the rate of 5% against the national average annualgrowth rate of 3.54%. The large population of low producing non-descript cattle, poor AIcoverage, unorganized dairy sector dominated by rural farmers, shrinkage in grazing land,acute shortage of quality breeding bulls, shortage of quality feed and fodder, poor milk collection,storage and marketing facilities, poor field data recording systems, climate change etc. aresome of the major issues to be resolved in near future for increasing the milk production to



vi

meet the demand of the country. Under such a scenario, cattle can contribute significantly tomeet this demand than buffalo as the scope for genetic improvement is more due to theavailability of wide unexplored genetic variation among the cattle breeds, huge population ofnon-descript cattle for up gradation and possibility of introduction of new superior germplasmavailable in other parts of the world.

The research efforts of Central Institute for Research on Cattle are primarily aimed tobring about sustainable improvement in cattle for high productivity and profitability. The primaryfocus of the Institute involves genetic upgradation of crossbred cattle, improvement ofindigenous cattle breeds and genetic evaluation of indigenous as well as crossbred cattle inthe field conditions. The Institute is also endeavoring to undertake research in physiological,nutritional, reproductive and managemental strategies for augmenting the cattle production.The whole genome based animal selection strategies, supply of sexed semen, application ofMOET, stem cell research, nanotechnology etc. will be explored in near future for increasingthe productivity and economic utility of cattle. The institute will also be a key player in thesocio-economic upliftment of cattle owners by infusing superior germplasm in the farmer’sherd, improving the economy of the cattle production by effective utilization of resources,production of quality clean milk and production of cattle byproducts etc. for enhanced income.

The most significant achievements made by the Institute during the last year in the areasof research, extension, administration and management are presented in the CIRC Annualreport 2014-15. I hope this will act as a useful reference material to the professionals, scientists,teachers, farmers, students, other government and non-governmental agencies and policymakers who are actively involved in cattle production.

At this juncture, I would like to express my sincere gratitude and acknowledgement for thevaluable guidance and support given by the Secretary, DARE and Director General, ICAR,the Deputy Director General (Animal Science) and their team at the ICAR HQ. Support receivedfrom the Director, Frieswal Project and the PIs of all co-operating centres of AICRP are thankfullyacknowledged. I record my sincere acknowledgment to the Heads of units, scientists,administrative and technical staff of the Institute for their contribution towards the progress ofthis Institute and also appreciate the efforts made by core committee of the Institute which isactively involved in the preparation of this document.

B.PRAKASHDIRECTOR

1



CIRC: AN INTRODUCTION

The Central Institute for Research onCattle (CIRC) formerly known as ProjectDirectorate on Cattle (PDC) was establishedon 3rd November 1987 at Military FarmsSchool and Research Centre, Meerut byupgrading the status of All-India CoordinatedResearch Project (AICRP) on Cattle. Sincethen the Directorate was actively collaboratingwith the Military Farms, Ministry of Defenceto evolve a national milch breed of crossbredcattle “Frieswal” by crossing the Sahiwal cattlewith Holstein Friesian bulls. Consideringimportance of the Indigenous cattle breeds,for their adaptability, feed conversionefficiency and disease resistance etc., theindigenous Breeds Project was undertakenin collaboration with State AgriculturalUniversities and State Government, Non-Government Organizations and sister ICARInstitutes for conservation and geneticimprovement of some of the importantindigenous cattle breeds of our country viz.,Gir, Sahiwal, Kankrej and Ongole. During the8th Five Year Plan the Field Progeny Testingprogramme (FPT) was also started toundertake progeny testing of crossbred bullsunder the field conditions. Considering itssincere research and extension efforts andachievements made in cattle improvement,the PD on Cattle was upgraded as CentralInstitute for Research on Cattle during 2014.Since then the CIRC is acting as a nodalinstitution to monitor, coordinate and supportall research and development projects forcattle improvement. The Institute is alsoproviding good quality germplasm tostakeholders. The Institute also obtained ISO9001:2008 certification and has wellequipped Semen Freezing and Molecular

Genetics laboratories besides feed testingfacilities in Animal Nutrition laboratory.

VISION

The vision of ICAR-CIRC is “Improvementof cattle for high productivity and profitability.”

MISSION

Germplasm improvement and technologydevelopment for realizing enhancedproductivity and profitability.

MANDATE

1. To undertake basic, applied and adaptiveresearch on all aspects of cattleproduction and reproduction and tostandardize package of practices.

2. To evolve a national milch breed of cattle‘FRIESWAL’, testing of crossbred bullsunder field conditions and to undertakeprogramme on genetic improvement ofindigenous breeds of cattle.

OBJECTIVES

AICRP on Cattle

1. To develop a national milch breed of cattle‘Frieswal’ using Holstein Friesian xSahiwal base.

2. Conservation and genetic improvementof important indigenous cattle breeds.

3. Production of progeny tested crossbredbulls and genetic improvement of cattleunder field conditions.



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ICAR-CIRC Main Scheme

1. To undertake research in the field of cattlebreeding, feeding, management andreproduction to enhance productivity andprofitability.

2. To plan, coordinate and monitor theresearch projects on cattle.

3. To serve as national data repository andprovide consultancy for cattle productionand reproduction.

FUTURE THRUST AREAS

1. Genetic improvement of other importantindigenous breeds of cattle viz.Tharparkar, Rathi, Red Sindhi, etc. usingconventional and modern breedingtechniques and studies on draught animalpower of important indigenous draughtbreeds.

2. Large scale production of quality cattlegermplasm.

3. Development of optimum feeding andmanagement practices includingdesigning of shelter to suit the localenvironment for enhancing cattleproductivity.

4. Sexing of male germplasm for productionof claves of desired sex.

5. Validation of therapeutic importance ofcow produces like milk, Punchgavaya,urine, dung etc.

6. Genomic selection of bulls and use ofembryo transfer technology for elite bullproduction.

7. Biotechnological strategies includingbiotic and abiotic stress for understandingand improving cattle production andreproduction.

8. Comparative economics of productivity ofIndian cattle breeds vis-à-vis crossbredcattle.

RESEARCH PROGRAMMES

AICRP on Cattle

1. Studies on genetic aspects of Holstein xSahiwal crossbreds- “FrieswalProject”.

2. Field recording of performance data forundertaking large scale progeny testing– “Field Progeny Testing of FrieswalBulls”.

3. Genetic studies on performance ofimportant indigenous breeds (Gir, Kankrejand Sahiwal) of cattle and theirimprovement through selection –“Indigenous Breeds Project”.

ICAR-CIRC Main Scheme

1. Increasing cattle productivity using latestbreeding tools.

2. Enhancement of cattle productivitythrough reproductive techniques.

3. Use of nutritional and managementinterventions for optimization of cattleproductivity.

4. HRD and technology dissemination.

3



INFRASTRUCTURE

Germplasm Resources

The Institute has undertaken researchprogrammes related to genetic improvementof indigenous and crossbred cattle byidentifying germplasm (G.P.) and datarecording (D.R.) units in various governmentand non-government organizations. WhileFrieswal cattle resource is available at 36Military Farms, the bull rearing unit is locatedat Meerut under the administrative control ofDirectorate of Frieswal. Similarily indigenouscattle genetic resources are available atdifferent G.P. and D.R. units of respectivebreeds. The germplasm unit for Sahiwal islocated at NDRI, Karnal while that of Gir, andKankrej are located at Junagadh AgriculturalUniversity, Junagadh and SK NagarUniversity, Dantiwada, respectively. Theinstitute has obtained ISO 9001:2008certification.

Research Laboratories

The Institute has well-equippedlaboratories to undertake basic and appliedresearch work in the fields of Animal Geneticsand Breeding, Animal Nutrition, AnimalPhysiology, Animal Reproduction andMolecular Genetics & Stem Cell Technology.Semen Freezing Laboratory of the Institutehas state of the art facilities with a capacityto store 25 lakh doses of frozen semen. Thelaboratory is an ISO:9001-2008 certified unit.

Computer Centre/ ARIS cell

Internet connectivity was provided to allstaff through Railtel under NKN, NIC, New

Delhi for smooth functioning of research workand FMS/ MIS beside other office work. Thewebsite of Institute was also designed inHindi and linked with existing websitewww.circ.org.in. The website is regularlyupdated from time to time. ARIS Cell lookedafter repairing and maintenance ofcomputers, printers, scanners and UPS etc.ARIS Cell procured new LCD Projector andsame was installed in conference hall. ARIScell also provided logistic support for variousaudio-visual presentations.

Library

A total of 22 Indian and 08 InternationalJournals were subscribed during the year2014-15. During this period, library procured238 (64 Hindi and 174 scientific) booksmaking a total of 1821 on its roll. Hindi (4)and English (3) daily newspapers along withliterary magazines and Hindi & Englishemployment newspapers were also madeavailable to the readers in the library. Libraryfacilities were also made available to sisterorganizations and students from SardarVallabh Bhai Patel University of Agricultrue& Technology, Meerut. Library organized twobook exhibitions during the period.



4

dk;Zdkjh lkjka'kEXECUTIVE SUMMARY

I. vf[ky Hkkjrh; lefUor xksoa'kvuqla/kku ifj;kstuk

I. AICRP ON CATTLE

xk; dh ladj iztkfr dk fodkl &Ýhtoky ifj;kstuk

Development of a crossbred strain ofcattle - Frieswal project

fofHkUu 36 lSU; QkeksZa ij 31 fnlacj 2014dks Ýhtoky iztkfr dh dqy 18624 eknk,amiyC/k FkhA ftuesa 10577 o;Ld xk;sa] 6118o`f)'khy eknk,a o 1929 cNfM+;k¡ FkhA lSU;QkeZ vEckyk ij lokZf/kd 2039 Ýhtokyeknk,a miyC/k FkhA tcfd fiEijh QkeZ ij;g la[;k 1633 o esjB lSU; QkeZ ij 1141FkhA fofHkUu lSU; QkeksZa ij laHkzkar eknkvksa dhla[;k 1111 FkhA esjB QkeZ ij lokZf/kd 102laHkzkar eknk,a Fkh tcfd vEckyk o y[kuÅQkeksZa ij ;g la[;k Øe'k% 96 o 97 FkhA

The total population of Frieswal femalesat 36 Military Farms at the end of 31st

December 2014 was 18624 including 10577adult cows, 6118 young stocks and 1929calves. The number of Frieswal females washighest at MF Ambala (2039) followed byPimpri (1633) and Meerut (1141). Thestrength of elite cows at various MilitaryFarms was 1111. MF Meerut (102) had thehighest number of elite cows followed byAmbala (96) and Lucknow (97).

esjB fLFkr lkaM ikyu bdkbZ ij vPNslkaM cukus ds mís'; ls ns'k ds fofHkUu lSU;QkeksZa ij laHkzkar laxe ls mRiUu dqy 1221cNM+s izkIr gq,A lSU; QkeZ vEckyk }kjklokZf/kd 283 Ýhtoky cNM+ksa dh vkiwfrZ dhxbZ tcfd lSU; QkeZ esjB o fiEijh }kjkØe'k% 230 o 153 cNM+s Hksts x,A vizSy 2014ls ekpZ 2015 rd dqy 100 cNM+s lkaM ikyubdkbZ ij izkIr gq,] ftlesa lSU; QkeZ] esjB lslokZf/kd 27] vEckyk ls 14 o tEew ls 13cNM+s izkIr gq,A

Since inception of the project, a total of1221 male calves born out by elite mating atMilitary Farms were received at BRU, Meerutfor rearing as future bulls. Military FarmAmbala had supplied highest number of malecalves (283) followed by Meerut (230) andPimpri (153). A total of 100 bull calves werereceived from different MFs during April 2014to March 2015 in which MF Meerut hadsupplied the highest number of male calves(27) followed by Ambala (14) and Jammu (13).

oh;Z fgehdj.k iz;ksx'kkyk esa fgehd`r oh;Zdh 3407000 ek=kvksa dk mRiknu vkjEHk ls31 ekpZ 2015 rd fd;k tk pqdk gSA xr o"kZ2014&15 esa dqy 337485 ek=k,a rS;kj dhxbZA ftlesa ls 67323 ek=k,a fofHkUu lSU;QkeksZa dks forjhr dh xbZA izfrosnu dky esafgehd`r oh;Z dh 71868 ek=k,a iSjk&osV]izknsf'kd i'kqikyu foHkkx] i'kq/ku fodkl cksMZ

5



o izknsf'kd d`f"k fo'ofo/kky;ksa dks csph xbZftlls dqy 1005732 #i;s dk jktLo izkIrgqvkA

Since inception of the project, a total of34,07,000 frozen semen doses (upto 31st

March 2015) have been produced. During thelast year (2014-15) a total of 3,37,485 dosesof semen were frozen and 67,323 doses havebeen distributed to Military Farms. During thereporting period 71,868 doses have beensold to para vets, State Animal HusbandryDepartments, Livestock DevelopmentBoards and State Agriculture Universities anda revenue of Rs. 10,05,732/- was generated.

Ýhtoky xk;ksa esa izFke C;kar dh vk;q dkek/; 973-25 fnu FkkA izFke C;kar dh vk;q ijQkeZ] ekSle o tUe o"kZ dk egRoiw.kZ izHkkoFkkA lalspu dky] 'kq"d dky o C;kar varjkydk U;wure oxZ ek/; Øe'k% 145-47] 104-43 o424-29 fnu FkkA lalspu dky] 'kq"d dky oC;kar varjky ij QkeZ] izlfork] ekSle o tUeo"kZ dk egRoiw.kZ izHkko FkkA rhu lkS fnuksa dkC;kar nqX/k mRiknu (MY300), dqy C;kar nqX/kmRiknu (TMY)] mPpre mRiknu (PY) onqX/kdky (LL) dk U;wure oxZ ek/; Øe'k%3284-78 fdxzk-] 3320-47 fdxzk-] 15-11 fdxzk-o 326-56 fnu FkkA bu xq.kksa ij QkeZ] izlfork]ekSle o tUe o"kZ o izFke C;kar ij vk;q dkegRoiw.kZ izHkko FkkA lSU; QkeZ dkuiqj ijÝhtoky xk; ls rhu lkS fnuksa esa lokZf/kdnqX/k mRiknu ¼4019-37 fdxzk-½ gqvk tcfdbykgkckn o egw lSU; QkeksZa ij ikyh xbZÝhtoky xk;ksa n~okjk rhu lkS fnuksa esa Øe'k%

3884-09 fdxzk- o 3863-21 fdxzk] nqX/k izkIrgqvkA

The overall mean of age at first calving(AFC) in Frieswal cows was 973.25 days.The effects of farm, season and year of birthwere significant on AFC. The least squaresmeans of service period (SP), dry period (DP)and calving interval (CI) were 145.47, 104.43and 424.29 days, respectively. The effects offarm, parity, season and year of calving weresignificant on SP, DP and CI. The overall leastsquares means of 300 days milk yield(MY300), total milk yield (TMY), peak yield(PY) and lactation length (LL) were 3284.78kg, 3320.47 kg, 15.11 kg and 326.56 days,respectively. The effects of farm, parity,season, year of calving and AFC weresignificant on these traits. Frieswal cows atMF Kanpur produced the highest MY300 of4019.37 kg followed by those maintained atAllahabad (3884.09 kg) and Mhow (3863.21kg).

izFke C;kar ij vk;q ¼0-052±0-010½] dqynqX/k mRiknu ¼0-054±0-009½] rhu lkS fnuksa dknqX/k mRiknu ¼0-046±0-009½ o mPpre nqX/kmRiknu ¼0-052±0-008½ ls lacaf/kr iSf=drkvkadyu de FkkA lalspu dky] 'kq"d dky oC;kar varjky dk iSf=drk vkadyu Hkh deFkk ¼0-007±0-005] 0-003±0-005 vkSj 0-007±

0-005½A

Estimates of heritability for AFC(0.052±0.010), TMY (0.054±0.009), MY300(0.0460±0.008) and PY (0.052±0.008) werelow. SP, DP and CI were also lowly heritable(0.007±0.005, 0.003±0.005 and0.007±0.005).



6

Ýhtoky i'kqvksa ds fyaxkuqikr esa cnykoik;k x;k rFkk 56-54 izfr'kr uj cNM+s iSnkgq,A dkbZ LDok;j x.kuk ls irk pyk fdfyaxkuqikr ij lalspu ds eghus o o"kZ vkSjizlfork dk dksbZ izHkko ugha Fkk tcfd QkeZdk egRoiw.kZ izHkko FkkA

The sex ratio of Frieswal animals wasfound altered and the proportion of malebirths was 56.54 per cent. Chi squareanalysis revealed no significant effect ofmonth and year of service, parity of animalson the sex ratio, while farm effect wassignificant.

{ksf=; ifjfLFkfr;ksa esa ladj xk;ksa dkvkuqoaf'kd lq/kkj

Genetic improvement of crossbred cattleunder field conditions

xq# vaxn nso i'kq&fpfdRlk o i'kq foKkufo'ofo|ky;] yqf/k;kuk

Guru Angad Dev Veterinary & AnimalSciences University, Ludhiana

vc rd 12 fofHkUu leqPp;ksa esa dqy 254lkaM iz;ksx fd;s tk pqds gSa o dqy 1]29]554xHkkZ/kku fd;s x, ftuls 16]185 cfNM+;k¡ mRiUugqbZ rFkk muesa ls 4]098 cfNM+;k¡ izFke C;kardh mez izkIr dj pqdh gSaA o"kZ 2014 esa dqy6]188 d`f=e xHkkZ/kku fd, x, o ldyxHkkZ/kku nj 46-4 izfr'kr vkadh xbZA o"kZ2014 esa ftu xk;ksa us izFke nqX/k lzo.kdkyiwjk dj fy;k Fkk muds izFke nqX/k lzo.kdkyds 305 fnuksa ds nqX/k mRiknu dk vkSlr

3703-6±31-3 fdyksxzke jgk o izFke C;kar ijvkSlr vk;q 1036-6 fnu ntZ dh xbZA fofHkUudkj.kksa ls 33-7 izfr'kr vkadM+ksa dk ºzkl gqvkftuesa i'kqvksa dk csp fn;k tkuk izeq[k dkj.kFkkA vkBosa] ukSosa o nlosa leqPp; ds lkaM+ksa lsmRiUu cNfM+;ksa ds nw/k esa vkSlr olk izfr'kr3-7 FkkA dqN izxfr'khy Msjh fdlkuksa us izf'k{k.ko ifj;kstuk ls mPpdksfV ds teZ IykTe dsfeyus ds mijkar viuk ladj xk;ksa dk ,slklewg fodflr dj fy;k ftlesa ,d C;kar esa6]000 fdyksxzke ls Hkh vf/kd nw/k nsus okyhxk;sa gSa vkSj buesa ls dqN xk;ksa dk mPprenqX/k mRiknu 40 yhVj@fnu ls Hkh vf/kd gSA{ks= larfr ijh{k.k dk;ZØe ds ckjs esa fdlkuksadks voxr djkus ds fy, xzke Lrj ij pkjlkewfgd cSBdsa Hkh vk;ksftr dh xbZ rFkkfdlkuks a dks oSKkfud fof/k ls iztuu]f[kykbZ&fiykbZ] izca/ku o LokLF; j{kk vkfnds ckjs esa le>k;k x;kA

A total of 254 bulls have so far beenintroduced in 12 different sets and of total1,29,554 inseminations done in which 16,185female progenies were born, out of which4,098 have reached age at first calving.During the year 2014, a total of 6,188 artificialinseminations were carried out and the overallconception rate was 46.4 %. Average firstlactation 305 days milk yield of cowscompleted their first lactation in the reportingperiod (2014) was 3703.6 ± 31.3 kg andaverage age at first calving was 1036.6 days.The per cent loss of data due to differentcauses was estimated as 33.7 and animalsale was the major among them. The averagefat percentage of milk of the daughters of

7



eighth, ninth and tenth set of bulls wasestimated as 3.7%. Some progressive dairyfarmers after getting training and superiorgermplasm from the project have establishedcrossbred herds with lactation milk yield ofmore than 6000 litres and peak yield of someof their cows is more than 40 litres a day. Fourvillage level group meetings were organizedto make the dairy farmers aware about theimportance of the field progeny testingprogramme and were advised for scientificbreeding, feeding, management and healthcare practices for improving the productionperformance of their animals.

dsjy i'kq&fpfdRlk o i'kq foKkufo'ofo|ky;] fFkzLlwj

Kerala Veterinary and Animal SciencesUniversity, Thrissur

rsjg fofHkUu leqPp;ksa esa vc rd 238lkaM iz;ksx fd;s tk pqds gSa o dqy 1]03]318xHkkZ/kku fd;s x, ftuls 7]756 cfNM+;k¡ mRiUugqbZ rFkk muesa ls 1]733 cfNM+;k¡ izFke C;kardh mez izkIr dj pqdh gSA o"kZ 2014 esa dqy4]408 d`f=e xHkkZ/kku fd, x, o ldyxHkkZ/kku nj 37 izfr'kr vkadh xbZA o"kZ 2014esa ftu xk;ksa us izFke nqX/k lzo.kdky iwjk djfy;k FkkA muds izFke nqX/k lzo.kdky ds305 fnuks a ds nqX/k mRiknu dk vkSlr2678-3±64-27 fdyksxzke jgk o izFke C;kar ijvk;q 1133-9 fnu ntZ dh xbZA fofHkUu leqPp;ksals mRiUu lrfr;k¡ ds e/; nqX/k mRiknu esaof) dh izofrZ ns[kh xbZA izFke leqPp; esa 300fnukas ds nqX/k mRiknu dk vkSlr 1958 fdyksxzkeFkk tks fd nlosa leqPp; esa c<+dj 2604fdyksxzke gks x;kA lqcg ds nw/k esa 2] 5 o 8

eghus ds lzo.kdky esa vkSlr olk izfr'krØe'k% 3-31] 3-42 o 3-66 FkkA larfr;ksa dhizFke C;kar ij vk;q 1009-52±29-77 fnu Fkhtksfd buds ledkyhu cNfM+;ksa esa 1250-10±23-38 fnu FkhA ifj;kstuk esa fofHkUu dkj.kksa lslkaMksa ds nlosa leqPp; esa 35-8 izfr'kr vkadM+ksadk ºzkl gqvk tcfd izfrosnu dky ds varrd X;kjgosa leqPp; ds lkaMksa esa vkadM+ksa dkºkl 27 izfr'kr gqvkA larfr;ksa dk nqX/klzo.kdky eas vkSlr mRiknu vius ledkyhuksals lkFkZd :i ls vf/kd FkkA

A total of 238 bulls have so far beenintroduced in 13 different sets and 1,03,318inseminations have been done in which 7,756female progenies were born, out of which1,733 have reached age at first calving.During the year 2014, a total of 4,408 artificialinseminations were carried out and the overallconception rate was 37%. Average firstlactation 305 days milk yield of cowscompleted their first lactation in the reportingperiod (2014) was found to be 2678.3 ± 64.27kg, while average age at first calving wasrecorded as 1133.9 days. The milk yieldshowed an increasing trend among theprogenies of different sets and average 305days milk yield increased from 1958 kg in 1st

set to 2604 kg in 10th set. Average fatpercentage of morning milk at 2, 5 and 8months of lactation was found to be 3.31, 3.42and 3.66, respectively. Average age at firstcalving of the progenies was found as1009.52 ± 29.77 days compared to 1250.10± 23.38 days for contemporaries. The lossof data in the project was 35.8% for 10th setof bulls and 27% for 11th set of bulls. Theaverage lactation yield of progenies wassignificantly higher than their contemporaries.



8

cSQ vuqla/kku fodkl QkmaMs'ku] mjyhdapu] iq.ks

BAIF Research DevelopmentFoundation, Uruli-Kanchan, Pune

X;kjg fofHkUu leqPp;ksa esa vc rd 230lkaM iz;ksx fd;s tk pqds gSa o dqy 94]423xHkkZ/kku fd;s x,A ftuesa 11]576 cfNM+;k¡mRiUu gqbZ ftuesa ls 4]126 cfNM+;k¡ izFkeC;kar dh mez izkIr dj pqdh gSaA o"kZ 2014 esadqy 5]771 d`f=e xHkkZ/kku fd, x, o ldyxHkkZ/kku nj 43-04 izfr'kr vkadh xbZA o"kZ2014 esa ftu xk;ksa us izFke nqX/k lzo.kdkyiwjk fd;k mudk 305 fnuksa ds nqX/k lzo.kdkydk vkSlr mRiknu 3206-64 fdyksxzke jgk oizFke C;kar ij vk;q 956-88 fnu ntZ dh xbZAlqcg ds nw/k esa 2] 5 o 8 eghus ds lzo.kdkyesa vkSlr olk izfr'kr Øe'k% 3-55] 3-59 o 3-61 FkkA larfr;ksa dh izFke C;kar ij vk;q1009-52±29-77 fnu Fkh tksfd buds ledkyhuksaesa 1250-10±23-38 fnu FkhA ifj;kstuk esa fofHkUudkj.kksa ls lkaMksa ds nlosa leqPp; esa 35 izfr'krvkadM+ksa dk ºkl gqvk tcfd izfrosnu dky dsvar rd X;kjgosa leqPp; ds lkaMksa esa vkadM+ksadk ºkl 35-4 izfr'kr gqvkA larfr;ksa dk vkSlrnqX/k lzo.kdky mRiknu vius ledkyhuksa esalkFkZd :i ls vf/kd FkkA izfrosnu dky esa243 lUrfr;ka izFke C;kar rd igqaph ftuesa ls157 us viuk izFke nqX/k lzo.kdky iwjk fd;kA

A total of 230 bulls have so far beenintroduced in 11 different sets and 94,423inseminations have been done in which11,576 female progenies were born, out of

which 4,126 have reached age at first calving.During the year 2014, a total of 5,771 artificialinseminations were carried out and the overallconception rate was 43.04 %. Average firstlactation 305 days milk yield of cowscompleted their first lactation in the reportingperiod (2014) was 3206.64 kg, while averageage at first calving was 956.88 days. Averagefat percentage for the morning milk of 2nd, 5th

and 8th month of lactation milk was 3.55, 3.59and 3.61, respectively. The loss of data was35% for 10th set of bulls and 35.4% for 11th

set of bulls. The average lactation yield ofprogenies was significantly higher than theircontemporaries. During the reporting perioda total of 243 progenies reached their firstlactation out of which 157 completed their firstlactation.

xksfoUn oYyHk iar d`f"k ,oa izks|kSfxdhfo'ofo|ky;] iaruxj

GBPUA &T, Pantnagar

pkj fofHkUu leqPp;ksa esa vc rd 40 lkaMiz;ksx fd, tk pqds gSa o dqy 9]496 xHkkZ/kkufd;s x,A vc rd 1]492 cfNM+;k¡ mRiUu gqbZftuesa ls 192 cfNM+;k¡ izFke C;kar dh mezizkIr dj pqdh gSaA o"kZ 2014 esa dqy 3]310d`f=e xHkkZ/kku fd, x, o ldy xHkkZ/kku nj56-7 izfr'kr vkadh xbZA o"kZ 2014 esa ftuxk;ksa us izFke nqX/k lzo.kdky iwjk dj fy;kFkk muds izFke nqX/k lzo.kdky ds 300 fnuksads nqX/k mRiknu dk vkSlr 2587-3 fdyksxzkejgk o izFke C;kar ij vk;q 1]044 fnu ntZ dhxbZA izFke leqPp; o f}rh; leqPp; ds lkaMksals mRiUu larfr;ksa esa 300 fnuksa ds nqX/k mRiknudk vkSlr Øe'k% 2545-9 o 2116-7 fdyksxzke

9



FkkA izfrosnu dky ds nkSjku lqcg ds nw/k esaizFke o f}rh; leqPp; ds lkaMksa ls izkIrlarfr;ksa esa vkSlr olk izfr'kr Øe'k% 3-8 o3-9 FkkA ifj;kstuk esa fofHkUu dkj.kksa ls lkaMksads izFke o f}rh; leqPp; esa vkadM+ksa dk ºklØe'k% 17-94 o 11-80 izfr'kr gqvkA izfrosnudky ds nkSjku {ks= esa nks vkSlj izn'kZuhvk;ksftr dh xbZA ftlesa yxHkx 250 fdlkuksaus Hkkx fy;kA fo'ofon~;ky; esa Hkh ,dxHkkZ/kku lg vkadM+k laxzgdrkZ izf'k{k.k dk;ZØevk;ksftr fd;k x;k ftlesa nl xHkkZ/kku lgvkadM+k laxzgdrkZ us d`f=e xHkkZ/kku o vkadM+klaxzg.k fof/k;ksa ij izf'k{k.k izkIr fd;kA

A total of 40 bulls have so far beenintroduced in 4 different sets and 9,496inseminations have been done in which 1,492female progenies were born, out of which 192have reached age at first calving. During theyear 2014, a total of 3,310 artificialinseminations were carried out and the overallconception rate was 56.7 %. Average firstlactation 300 days milk yield of cowscompleted their first lactation in the reportingperiod (2014) was 2587.3 kg, while averageage at first calving was recorded as 1044days. The average 305 days milk yield of theprogenies of first and second set of bulls were2545.9 and 2116.7 kg, respectively. Averagefat percentage of morning milk during thereporting period were as 3.8, and 3.9 for thefirst and second set of bulls. The per cent lossof data was estimated as 17.94 for first setof bulls and 11.80 for second set of bulls,respectively. During the reporting period twoheifer shows were conducted in the field andnearly 250 farmers actively participated in theprogramme. The University also arrangedone inseminator cum data recorder training

program in which 10 inseminators cum datarecorders were given training on AI and datarecording methods.

xk; dh ns'kh uLyksa dk laj{k.k o vkuqoaf'kdlq/kkj

Conservation and genetic improvementof indigenous cattle breeds

fxj uLy

Gir breed

teZIykTe bdkbZ ij 31 fnlEcj 2014 dksiztuu ;ksX; eknkvksa dh la[;k 67 Fkh ftuesa28 vkSljsa Hkh lfEefyr gSaA bl dk;ZØe esalaxfBr QkeksZa ij 388 o {ks= bdkbZ;ksa ij10]285 iztuu ;ksX; xk;ksa dks feydj dqy10]673 iztuu ;ksX; xk;ksa dks fpfUg~r fd;kx;kA bl dk;ZØe esa vc rd nks leqPp;ksa esadqy 15 lkaM iz;ksx fd;s tk pqds gSaA ftuesaizFke leqPp; esa 6 o f}rh; leqPp; esa 9lkaM iz;ksx fd;s x,A o"kZ 2014 ds izkjaHk esabl dk;ZØe ds varxZr fgehd`r oh;Z dh dqy14]676 ek=k,a miyC/k Fkh rFkk bl o"kZ fgehdroh;Z dh dqy 10]097 ek=k,a rS;kj dh xbZAftuesa ls fgehd`r oh;Z dh 3]928 ek=k,axHkkZ/kku gsrq iz;ksx dh xbZ o dqy 758 ek=k,acsph xbZ o 31 fnlEcj 2014 dks dqy 20]087ek=k,a miyC/k FkhA o"kZ 2014 esa dqy 2]929i'kqvksa esa d`f=e xHkkZ/kku fd;k x;k oxHkkZ/kkj.k nj 46-12 izfr'kr ikbZ xbZA vkjaHkls vc rd izFke leqPp; o f}rh; leqPp; esaØe'k% 11]168 o 4]376 xHkkZ/kku fd;s x, oxHkkZ/kkj.k nj Øe'k% 50-64 o 41-06 izfr'kr



10

jghA o"kZ 2014 esa dqy 587 cNfM+;k¡ iSnk gqbZvFkkZr dk;ZØe ds izkjaHk ls vHkh rd dqy3]346 cNfM+;k¡ iSnk gks pqdh gSaA

The number of breedable females in theGP unit as on 31st December 2014 was 67(including 28 heifers). Nearly 10673breedable females were identified under theproject at organized farms (388) as well asfield units (10285). Fifteen Gir bulls in two sets(six in set-I and nine in set-II) have beeninducted so far. At the start of the year 2014a total of 14676 doses were in stock andduring the year 10097 semen doses wereproduced, 3928 doses were utilized forinsemination, 758 doses were sold and20087 doses were available as on 31st

December 2014. During the year 2014 a totalof 2929 animals were inseminated and theconception rate was found to be 46.12 percent. The number of inseminations carried outsince inception for set-I and set-II were 11168and 4376 resulting to a conception rate of50.64 and 41.06 per cent, respectively.During the year 2014 a total of 587 daughterswere born which resulted in to a total of 3346daughters since inception.

teZIykTe bdkbZ ij izFke C;kar ds nqX/kmRiknu dk vkSlr 2731-90±437-60 fdyksxzkeFkkA tcfd leLr nqX/k lzo.kdky ds nqX/kmRiknu dk vkSlr 2572-50± 264-20 fdyksxzkeik;k x;kA vkSlr izFke nqX/k lzo.kdky 408fnu ik;k x;kA izFke C;kar ij vk;q] izFkelalspu dky] izFke 'kq"d dky o C;kar varjkydk vkSlr Øe'k% 1284-50±31-30] 139-10±9-30]200-20±33-70] 552-00± 46-80 fnu ik;k x;kAteZ IykTe bdkbZ dk oSV o MªkbZ vkSlr Øe'k%7-40±0-60 o 4-70±0-50 fdyksxzke ik;k x;kA

The overall average first lactation milkyield of GP unit was estimated as 2731.90 ±437.60 kg while average milk yield for alllactation was found to be 2572.50 ± 264.20kg. The average lactation length and peakyield were recorded as 374.30 ± 20.60 daysand 13.90 ± 0.60 kg, respectively. The overallage at first calving, first service period, firstdry period, first lactation length and calvinginterval were found to be 1284.50 ± 31.30,139.10 ± 9.30, 200.20 ± 33.70, 408 and552.00 ± 46.80 days, respectively. The wetand dry averages of the GP unit wereestimated as 7.40 ± 0.60 and 4.70 ± 0.50kg, respectively.

dkadjst uLy

Kankrej breed

teZIykTe bdkbZ o vkadM+k laxzg.k bdkbZ¼laxfBr QkeZ o QkeZ bdkb;ksa dks feykdj½ij 31 fnlEcj 2014 dks iztuu ;ksX; eknkvksadh la[;k Øe'k% 67 o 3]058 FkhA bl dk;ZØeesa vc rd nks leqPp;ksa esa dqy 17 lkaM iz;ksxfd;s tk pqds gSaA izFke leqPp; esa cukl Msjhds vkB lkaM Fks rFkk f}rh; leqPp; esa 9 lkaMFksA o"kZ ds izkjaHk esa bl dk;ZØe ds varxZrfgehd`r oh;Z dh dqy 66]623 ek=k,a miyC/kFkh rFkk bl o"kZ fgehd`r oh;Z dh dqy 33]670ek=k,a rS;kj dh xbZA f}rh; leqPp; ds lkaMksads fgehd`r oh;Z dh 3]975 ek=k,a lekxe gsrqiz;ksx dh xbZ o 2]410 ek=k,a csph xbZ o o"kZds var rd fgehd`r oh;Z dh 80]390 ek=k,amiyC/k FkhA bl dky esa 2]286 i'kqvksa esad`f=e xHkkZ/kku fd;k x;kA ftlesa ls 893i'kqvksa us xHkkZ/kkj.k fd;k o dqy 252 cNfM+;k¡iSnk gqbZA o"kZ 2014 esa vkSlr xHkkZ/kkj.k nj

11



39-06 izfr'kr FkhA tcfd ldy xHkkZ/kkj.knj 46-45 izfr'kr FkhA izFke leqPp; ds lkaMksals 392 o f}rh; leqPp; ds lkaMksa ls 475cNfM+;k¡ iSnk gqbZ vFkkZr dk;ZØe ds izkjaHk lsvHkh rd dqy 867 cNfM+;k¡ iSnk gks pqdh gSaA

The number of breedable females in GPand DR units as on 31st December, 2014were 67 and 3058 (Includes organized farmand field units), respectively. Seventeen bullsin two sets have been inducted so far andthe first set consisted of eight bulls from BanasDairy while the second set consisted of ninebulls. At the start of the year, 66623 semendoses were available under the project andduring the year 33,670 doses were producedand 3975 doses of second set of bulls wereutilized for insemination and 2410 were soldresulting in a balance of 80390 semendoses. During the reporting period 2286animals were inseminated, 893 animalswere confirmed for pregnancy and 263daughters were born. The conception rateduring the year 2014 was 39.06 per centagainst overall rate of 46.45 per cent. Thenumber of daughters born for the first andsecond set of bulls was 392 and 475,respectively resulting in 867 Kankrejdaughters since inception of the project.

teZIykTe bdkbZ ij izFke C;kar ij vk;q]izFke lalspu dky] izFke 'kq"d dky o C;karvarjky dk ldy vkSlr Øe'k% 1398-75±

80-40] 187-00±75-99] 109-20±8-90 o 431-35±21-42 fnu ik;k x;kA tcfd izFke C;kar dsnqX/k mRiknu] leLr nqX/k lzoudky dk nqX/kmRiknu o izFke mPpre nqX/k mRiknu dkvkSlr Øe'k% 2136-04±195-61] 2760-71±

134-67 o 10-59±0-33 fdyksxzke ik;k x;kAizFke C;kar dk nqX/k mRiknu] leLr nqX/klzo.kdky ds nqX/k mRiknu ls de Fkk tks fd;g iznf'kZr djrk gS fd dkadjst xk;ksa esavuqorhZ nqX/k lzo.kdkyksa ds nqX/k mRiknu esao`f) gqbZ gSA lewg dk vkSlr izFke nqX/klzo.kdky 288-20±15-96 fnu ik;k x;kA tcfdoSV o MªkbZ vkSLkr Øe'k% 9-14 o 6-86 fdyksxzkeik;k x;kA

The overall average estimates for age atfirst calving, first service period, first dryperiod and calving interval in GP unit were1398.75 ± 80.40, 187.00 ± 75.99, 299.25 ±56.17 and 431.35 ± 21.42 days, respectivelywhile average estimates for first lactation milkyield, all lactation milk yield and first peakyield were recorded as 2136.04 ± 195.61,2760.71 ± 134.67 and 10.59 ± 0.33 kg,respectively. The average first lactation milkyield was lower than all lactation milk yieldindicating improvement in the milk productionin the subsequent lactations in Kankrej cattle.The average first lactation length of the herdwas 288.20 ± 15.96 days while the wet anddry averages were 9.14 and 6.86 kg,respectively.

lkghoky uLy

Sahiwal breed

teZIykTe bdkbZ ij nks o"kZ ls vf/kd vk;qdh iztuu ;ksX; eknkvksa dh la[;k 214 ovkadM+k laxzg.k bdkbZ ij 599 FkhA bl dk;ZØeesa vc rd nks leqPp;ksa esa dqy 15 lkghokylkaMksa dk iz;ksx fd;k tk pqdk gSA ftuesaizFke leqPp; eas 8 o f}rh; leqPp; esa 7



12

lkaM iz;ksx fd;s x,A o"kZ 2014 ds izkjaHk esabl dk;ZØe ds varxZr fgehd`r oh;Z dh dqy49]236 ek=k,a miyC/k Fkh rFkk bl o"kZ fgehdroh;Z dh dqy 23]840 ek=k,a rS;kj dh xbZAftuesa ls 5]061 ek=k,a fgr /kkjdksa dks csphxbZ o 8]201 ek=k,a xHkkZ/kku gsrq iz;ksx dhxbZA orZeku esa dqy 59]814 ek=k,a miyC/kgSaA

The number of breedable females abovetwo years of age in the GP unit was 214 andthe corresponding number in the DR unit was599. Fifteen Sahiwal bulls in two sets (8 infirst and 7 in second set) have been inductedso far in the project. At the start of year 2014a total of 49236 semen doses were in stockand 23840 doses were produced. A total of5061 doses were sold to the stakeholdersand 8201 doses were supplied forinsemination resulting to a total balance of59814 doses.

izfrosnu dky esa 1]406 xHkkZ/kku fd;s x,ftUgsa feykdj vkjaHk ls vc rd dqy 5]276xHkkZ/kku fd;s tk pqds gSaA o"kZ 2014 esa lkaMksads izFke o f}rh; leqPp; esa ldy xHkkZ/kkunj Øe'k% 32-56 o 41-45 izfr’kr vkadh xbZtcfd ldy xHkkZ/kku nj 41-18 izfr'kr FkhAvkjaHk ls vc rd izFke o f}rh; leqPp; esaxHkkZ/kku nj Øe’k% 36-69 o 40-33 izfr'krvkadh xbZ tcfd ldy xHkkZ/kku nj 37-85izfr’kr FkhA

During the reporting period 1406inseminations were carried out amounting toa total insemination of 5276 since inception.

During the year 2014, conception rates forfirst and second set of bulls were 32.56 and41.45 per cent, respectively with an overallconception rate of 41.18 per cent. Theconception rate since inception was 36.69and 40.33 for the first and second set of bullsamounting to an overall conception rate of37.85 per cent. So far 681 Sahiwal daughterswere born under the project while during thereport period 184 daughters were born.

teZIykTe bdkbZ ij j[kh xbZ lkghokyxk;ksa esa izFke C;kar ij vk;q] izFke lalspudky] izFke 'kq"d dky o C;kar varjky dkldy vkSlr Øe'k% 1238-22±27-55] 138-75±24-48] 123-13±33-27 o 425-38±425-41 fnuik;k x;kA tcfd izFke C;kar ds 305 fnuksa dknqX/k mRiknu] izFke C;kar ds dqy nqX/k mRiknuo izFke mPpre nqX/k mRiknu dk vkSlr Øe'k%2054-92±229-49] 2437-79±379-27 o 11-41±

0-69 fdyksxzke ik;k x;kA lewg dk vkSlrizFke nqX/k lzo.kdky 316-53±31-69 fnu ik;kx;kA tcfd oSV o MªkbZ vkSlr Øe'k% 7-17 o3-47 fdyksxzke ik;k x;kA

The overall average estimates for age atfirst calving, first service period, first dryperiod and calving interval of Sahiwal cowsmaintained at GP unit were 1238.22 ± 27.55,138.75 ± 24.48, 123.13 ± 33.27 and 425.38± 25.41 days, respectively while averageestimates for first lactation 305-day milk yield,total first lactation milk yield and first peakyield were 2054.92 ± 229.49, 2437.79 ±379.27 and 11.41 ± 0.69 kg, respectively. Theaverage first lactation length of the herd was316.53 ± 31.69 days while the wet and dryaverages were 7.17 and 3.47 kg, respectively.

13



II. laLFkkfud dk;ZØe

II. INSTITUTIONAL PROGRAMME

Ýhtoky lkaMksa esa oh;Z mRiknu o oh;Z dkvkdkjferh; pfj= fp=.k

Semen Production and sperm biometricsin Frieswal bulls

Ýhtoky lkaMksa ls vizSy 2014 ls ekpZ2015 ds e/; izkIr 8]471 L[kyuksa esa vkSlroh;Z vk;ru ¼fe-yh-½] 'k qØk.k q lk an zrk¼fefy;u@fe-yh-½] vkjafHkd xfr’khyrk¼izfr'kr½ o iksLV&Fkk xfr'khyrk ¼izfr'kr½Øe'k% 4-73±0-02] 960-18±5-49] 53-45±0-24 o 41-71±0-30 FkhA

The average semen volume (ml), spermconcentration (million/ml), initial motility (%)and post thaw motility (%) was 4.73 ± 0.02,960.18 ± 5.49, 53.45 ± 0.24 and 41.71 ±0.30, respectively in 8471 ejaculatescollected from Frieswal bulls during April2014-March 2015.

iksLV&Fkk xfr'khyrk ¼izfr’kr½] lkB feuVds Å"ek;u ds i’pkr xfr’khyrk ¼izfr’kr½]HOST (%)] vfody ,Økslkse (%), o 'kqØk.kqlkanzrk ¼fefy;u@LVªk½ Øe'k% 54-57±0-64]37-23±0-73] 56-18±1-14] 69-06±0-65 o 26-75±0-61 FkhA

Post thaw motility (%), motility afterincubation of 60 min (%), HOST (%), intactacrosome (%) and sperm concentration(million/straw) was 54.57±0.64, 37.23±0.73,56.18±1.14, 69.06±0.65 and 26.75±0.61,respectively.

u, rFkk leL;kxzLr lkaMksa esa thfor 'kqØk.kq(%) rFkk dqy 'kqØk.kq folaxfr;k¡ (%) Øe'k%72-58± 2-48 o 15-01±1-13 vkSj 64-62±7-59 o26-91 ±6-82 FkhA

Live spermatozoa (%) and overallabnormality (%) in the semen of new andproblem bulls was 72.58±2.48 & 15.01±1.13and 64.62±7.59 & 26.91±6.82, respectively.

iSarhl lkaMksa ds oh;Z uewuksa ds izkjafHkdfo'ys"k.k ls Kkr gqvk fd 'kqØk.kq dh yEckbZ]pkSM+kbZ] ifjf/k o {ks=Qy Øe'k% 8-93] 5-89]23-91 ekbØksehVj o 36-22 oxZ ekbØksehVjFksA

Preliminary analysis of semen samplesfrom 35 bulls showed that mean values forlength, width, perimeter and area of spermswere 8.93, 5.89, 23.91 µm and 36.22 µm2,respectively and the mean ellipticity, rugosity,elongation & regularity measures were 1.58,9.49, 0.20 and 1.14, respectively.

Ýhtoky lkaMksa dh vkuqoaf'kd jksxksa dsfo#) ij[k

Screening of Frieswal bulls for geneticdisorders

Ýhtoky cNM+ksa dks cksokbZu Y;wdkslkbVvMghtu fMfQfl;salh (BLAD)] fMfQfl;salhvkWQ ;qjhMhu eksuksQkLQsV flaFkst (DUMPS)]cksokbZu flV#fuysfe;k o QSDVj XI fMfQfl;salhds fo#) ij[kk x;kA ,d lkS bD;kou r#.kcNM+ksa ds [kwu ds uewus ,df=r fd;s x, oij[ks x,A ,xjkst tSy ds cSafMax iSVuZ dsvk/kkj ij thuksVkbZfiax fd;k x;kA cksokbZu



14

Y;wdkslkbV vMsfglu fMfQfl,salh fcekjh dsfy, Ýhtoky cNM+ksa esa 4-6% laokgd izpkyuns[kk x;kA tcfd vU; vkuqoaf'kd chekfj;ksads fy, dksbZ laokgd ugha ik;k x;kA

Frieswal bull calves were screened againstBovine leukocyte adhesion deficiency (BLAD),deficiency of uridine monophosphate synthase(DUMPS), bovine citrullinaemia and factor XIdeficiency. Blood samples of 151 young bullswere collected and screened. Genotyping wasdone based on the banding pattern in agarosegel. A carrier prevalence of 4.6 percent wasnoticed for BLAD in Frieswal bull calves whileno carriers were noticed for other geneticdisorders studied.

iztuu rduhdksa] iks"k.k o izcU/ku gLr{ksiksa}kjk vkSljksa dh iztuu n{krk esa o`f)

Enhancing reproductive efficiency byreproductive techniques, nutrition andmanagement interventions

Ýhtoky cfN;k s a dh O; fDrxrf[kykbZ&fiykbZ ds dkj.k en izn'kZu tYnhns[kk x;k rFkk vf/kd xHkkZ/kku nj ¼90%½ikbZ xbZA cfN;ksa esa C;kus ds mijkUr izFkeen~dky izn'kZu ds le; vkSlr 'kjhj Hkkj388-55±8-53 fdyksxzke Fkk rFkk d`f=exHkkZ/kku ds le; 'kjhj Hkkj 399-62±9-77fdyksxzke FkkA ftu Ýhtoky xk;ksa dhO;fDrxr f[kykbZ&fiykbZ djkbZ xbZ FkhA muesanqX/k lzo.kdky ds izkjaHk gksus ds le; vkSlrnsg Hkkj 407-2 fdyksxzke Fkk tksfd o"kZ Hkjdk;e jgkA 12 eghuksa ds ckn vkSlr nsg Hkkj418 fdyksxzke FkkA

Individual feeding of postpartum Frieswalcows resulted into early exhibition of estrusand higher conception rates (90%). The meanbody weight at the time of first estrusexhibition after calving was 388.55 ± 8.53 kgand body weight at the time of AI was 399.62± 9.77 kg. The average body weight ofFrieswal cows kept on individual feeding atthe start of lactation was 407.22 kg and theymaintained the body weight throughout theyear. The mean body weight after the end of12 months period was 418.0 kg.

iztuu rduhdksa ,oa iks"k.k gLr{ksiksa }kjkxzkeh.k {ks=ksa ds xksi'kqvksa dh iztuu n{krkesa o`f) djuk

Augmenting the reproductive efficiencyof cattle in rural areas

bl vof/k esa vjukoyh] iwB[kkl] ektjk]jlwyiqj rFkk cjukok xkao esa 06 cka>iu fuokj.kf'kfoj vk;ksftr fd, x,A bu f'kfojksa eas 317i'kqvksa dk ijh{k.k o bykt fd;k x;kA nksf'kfoj iapk;rh xkS'kkyk] gkiqM+ esa Hkh vk;ksftrfd;s x,A buesa dqy 58 i'kqvksa dk fofHkUuchekfj;ksa ds fy, ijh{k.k fd;k x;kA dqyfeykdj 30 izfr'kr eknk i'kqvksa esa enghurkns[kh x;h rFkk 15-14 izfr'kr i'kq fjihV czhMjFksA yxHkx 45 izfr'kr i'kq vU; chekfj;ksa lsxzflr FksA enghurk okys i'kqvksa dk mipkjfeujy feDlpj rFkk izkstsLVªksu ,oa bZLVªkstudks la;qDr :i ls f[kykdj fd;k x;kA blmipkj ls 63 izfr'kr i'kqvksa esa enghurk dkfuokj.k ik;k x;kA

Six Infertility camps were organised atArnavali, Pooth Khas, Majra, Rasulpur and

15



Barnava villages during this period and 317animals were examined and treated. Twocamps were also organized at Panchayatigaushala Hapur where 58 animals wereexamined. A total of 30% animals sufferedfrom anoestrus and 15.14% were repeatbreeders. Approximately 45% of animalswere suffering from other diseases.Anoestrus animlas were given a combinedpreparation of mineral mixture and hormonesorally (progesterone + estrogen) and 63% ofthese animals responded to treatment.

lw{eekf=d [kfutksa ds fofHkUu Lrjksa dkÝhtoky lkaMksa ds oh;Z dh xq.kkRed oek=kRed fo'ks"krkvksa ij izHkko

Effect of different levels of micro-mineralson qualitative and quantitative attributesof semen in Frieswal bulls

cf<+;k oh;Z nsus okys lkaMksa esa izfr fdyksxzkemikip;h otu ij 'kq"d inkFkZ varxzZg.k vkgkj1] vkgkj 2 o vkgkj 3 ij Øe'k% 73-65±2-11]76-49±1-82 o 70-65±2-80 xzke FkkA tcfd?kfV;k oh;Z nsus okys lkaMksa esa ;g Øe'k%77-42±3-61] 80-82±3-26 o 76-31±2-28 xzkeFkkA 'kq"d inkFkZ dh ipuh;rk lHkh lewgksa dslHkh iz;ksxkRed lkaMksa esa yxHkx leku FkhA

Dry matter intake per kg metabolic weightof good quality semen bulls was 73.65±2.11,76.49±1.82 and 70.65±2.80 gram on diet 1(40 ppm Zn), diet 2 (60 ppm Zn) and diet 3(80 ppm Zn), respectively; while in poorsemen quality bulls the corresponding valueswere 77.42±3.61, 80.82±3.26 and76.31±2.28 gram. Dry matter digestibility inthe experimental bulls was almost similar inall the groups.

izlkj xfrfof/k;k¡

Extension Activities

laLFkku us izfrosnu dky esa fofHkUu laLFkkuksa}kjk vk;ksftr fd;s x;s lkr fdlku esyksa oizn'kZfu;ksa esa Hkkx fy;kA bu esyksa esa gekjslaLFkku dh izeq[k xfrfof/k;ksa o gLrkarfjddh tkus okyh rduhfd;ksa ds ckjs esa crk;kx;kA fofHkUu fdlku esyksa esa laLFkku ds LVkyij 2]300 ls vf/kd fdlkuksa us tkudkjh izkIrdhA tu dY;k.k laLFkk] esjB o ukckMZ] esjBds lg;ksx ls ,d fnolh; i'kq ikyu tkx:drkf'kfoj dk vk;kstu fd;k x;kA laLFkku }kjk6 i'kq LokLF; o cka>iu fuokj.k f'kfoj Hkhvk;ksftr fd;s x;s rkfd fdlkuksa dh i'kqvksals lacaf/kr iqu#Riknu o vU; lacaf/kr leL;kvksadk fuokj.k fd;k tk ldsA blds vfrfjDrlaLFkku ds oSKkfudksa }kjk vaxhd`r dh xbZ nksxkS'kkykvksa esa fu;fer :i ls Hkze.k fd;kx;k rFkk ogka ds lHkh i'kqvksa dh fofHkUuchekfj;ksa dk lQyrk iwoZd bykt fd;k x;kA

During the report period the Instituteparticipated in 7 Kisan Melas organized bydifferent Institutions. Major activities andtransferrable technologies developed by theInstitute were depicted at ICAR-CIRC Stall inthe Melas. More than 2300 farmers visitedCIRC stall during different Melas. A one dayAnimal Husbandry Awareness Camp wasorganized in collaboration with Jan KalyanSanstha, Meerut and NABARD, Meerut. Atotal of 6 Animal Health cum Infertility Campswere organized by the Institute to help farmersin solving their animal related reproductiveand associated problems. In addition to this,regular visits to two Gaushalas adopted bythe Institute were performed by the scientistsof the Institute.



16

17



STAFF POSITION

Sl.No. Category Sanctioned Filled Vacant

1. R.M.P. 01 01 00

2. Scientific 40 23 17

3. Technical 09 08 01

4. Administrative 14 13 01

5. Supporting 10 10 00

Total 74 55 19

FINANCIAL STATEMENTICAR-CIRC

CIRC NON PLAN CIRC PLAN AICRP on Cattle ITMU

Sl. Head of Accounts RE Expdtr RE Expdtr RE Expdtr RE ExpdtrNo. 2014-15 2014-15 2014-15 2014-15

1 2 3 4 5 6 7 8 9 10

Grant in AID CAPITAL Assets

1 Works

A) Land 0 0 0 0 0 0 0 0

B) Buildings & other original work 0 0 0 0 0 0 0 0

i) Office buildings 0 0 0 0 0 0 0 0

ii) Residential buildings 0 0 0 0 0 0 0 0

iii) Minor works 0 0 0 0 0 0 0 0

2 Equipments 200000 102800 3410000 2959595 0 0 0 0

3 Information technology 0 167000 166800 0 0 0 0

4 Library books/journals 0 1200000 1199041 0 0 0 0

5 Vehicles Including cycles 0 0 0 0 0 0 0

6 Livestock 0 0 0 0 0 0 0

7 Furniture, fixtures & fittings 100000 99850 33000 32800 0 0 0 0

8 Others 0 0 290000 206320 0 0 0 0

Total (CAPITAL Expenses) 300000 202650 5100000 4564556 0 0 0 0

Grant in Aid - Salaries (REV)

1 Establishment Expenses

(a) i) Establishment charges 50060000 50030224 0 0 17850000 17850000 0 0

ii) Wages 0 0 0 0 0 0 0 0

iii) Overtime allowance 0 0 0 0 0 0 0 0

Total - Estt. Expenses 50060000 50030224 0 0 17850000 17850000 0 0



18

CIRC NON PLAN CIRC PLAN AICRP on Cattle ITMU

Sl. Head of Accounts RE Expdtr RE Expdtr RE Expdtr RE ExpdtrNo. 2014-15 2014-15 2014-15 2014-15

1 2 3 4 5 6 7 8 9 10

Grant in Aid - General (REV)

1 Pension & other ret. benefits 150000 147047 0 0 0 0 0 0

2 Travelling Allowance

(a) Domesitc TA/Transfer TA 900000 899969 400000 396395 615000 615000 70000 27814

(b) Foreign TA 0 0 0 0 0 0 0 0

Total : Travelling Allowances 900000 899969 400000 396395 615000 615000 70000 27814

3 Research & Operational Expenses

(a) Research Expenses 525000 523310 2396000 2395435 20415000 20415000 0 0

(b) Operational expenses 850000 849326 2549000 2547547 10230000 10230000 480000 394405

Total: Res.& Oprnl Expenses 1375000 1372636 4945000 4942982 30645000 30645000 480000 394405

4 Administrative Expenses

(a) Infrastructure: 1725000 1712579 900000 893663 0 0 0 0

(b) Communications 55000 54945 80000 70023 0 0 0 0

(c) Repairs & maintenance:

i) Equipments, vehicles & others 440000 439943 150000 149065 0 0 0 0

ii) Office buildings 845000 844112 500000 499531 0 0 0 0

iii) Residential buildings 125000 125000 400000 399601 0 0 0 0

iv) Minor works 150000 150000 0 0 0 0 0 0

(d) Others (excluding TA) 600000 599960 900000 894175 890000 890000 0 0

Total: Administrative Expenses 3940000 3926539 2930000 2906058 890000 890000 0 0

5 Miscellaneous expenses

(a) HRD 0 0 325000 316281 0 0 0 0

(b) Other (Fellow/Scholarships) 0 0 0 0 0 0 0 0

(c) Publicity & exhibitions 100000 95987 0 0 0 0 0 0

(d) Guest house - maintenance 0 0 0 0 0 0 0 0

(e) Other miscellaneous 125000 120177 300000 292299 0 0 0 0

Total: Miscellaneous expenses 225000 216164 625000 608580 0 0 0 0

Total Grant-in-Aid : General 6590000 6562355 8900000 8854015 32150000 32150000 550000 422219

Total: Grant (Salaries + General) 56650000 56592579 8900000 8854015 50000000 50000000 550000 422219

Grand Total (Capital + Revenue) 56950000 56795229 14000000 13418571 50000000 50000000 550000 422219

D.S.T. PROJECT RE 2014-15 Expenditure

i) Fellowship @ Rs.35000/- PM 420000 420000

ii) Consumables 200000 199388

iii) Contingencies 25000 24994

iv) Travel Expenses 25000 2410

v) Overhead Expenses 100000 93799

Total 770000 740591

Resource Generation: Target- 8.60 Lakh Achievement- 6.80 Lakh

Externally Funded Programme

19



RESEARCH ACHIEVEMENTS

(I) AICRP ON CATTLE

(A) DEVELOPMENT OF CROSSBREDSTRAIN OF CATTLE: FrieswalProject

Frieswal is a crossbred cattle having 5/8Holstein Friesian and 3/8 Sahiwal blood,developed by Central Institute for Researchon Cattle, Meerut, in collaboration withMinistry of Defense. The Frieswal projectenvisages evolving a National Milch Breed“Frieswal”, a Holstein- Sahiwal cross, yielding4000 kg of milk with 4% butter fat in a maturelactation of 300 days. The evolution ofFrieswal has been progressing by utilizingthe existing crossbred herds available at 36Military Farms located in various agro-climatic regions of the country.

Population structure

Herd strength

The total population (31st December2014) of Frieswal females at 36 MilitaryFarms located in various agro-climaticregions of the country was 18,624 including10,577 adult cows, 6,118 young stocks and

1,929 calves (Table-1). The number ofFrieswal females was highest at MF Ambala(2,039) followed by Pimpri (1,633) andMeerut (1,141). At Bull Rearing Unit (BRU),Meerut a total of 276 Frieswal bulls (as on31st March 2015) including 217 adult, 53young stock and 06 calves are also beingmaintained. A total of 1,111 elite Frieswalcows (10.50% of the adult cows) wereselected as bull mothers for nominatedmatings (Table-2). MF Meerut (102) had thehighest number of elite cows followed byLucknow (97) and Ambala (96).

A total of 1,221 male calves, born out byelite mating at Military Farms in differentyears, true to breed characteristics and withgood physical conformity were received atBRU, Meerut for future breeding sinceinception of the project, MF Ambala suppliedthe highest number (283) of male calvesfollowed by Meerut (230) and Pimpri (153).A total of 100 bull calves were received fromdifferent MFs during April 2014 to March 2015(Table-3). The MF Meerut had suppliedhighest number of male calves (27) followedby Ambala (14) and Jammu (13).

An elite Frieswal cow Male calves reared at BRU unit



20

Table 1. Female herd strength of Frieswal at various Military Farms (2014-15)

Farm Adults YS-II YS-I Calves Total

Meerut 691 149 174 127 1141

Agra 184 130 63 31 408

Lucknow 438 125 83 85 731

Bareilly 195 61 52 36 344

Kanpur 173 55 32 20 280

Jabalpur 278 158 58 58 552

Namkum 345 143 102 61 651

Mhow 150 41 24 09 224

Allahabad 214 80 38 32 364

Ranikhet 67 0 0 0 67

Ambala 1060 481 267 231 2039

Jalandhar 635 275 97 126 1133

Ferozpur 287 99 53 53 492

Pathankot 258 109 57 55 479

Dagshai 149 0 0 0 149

Yol 107 08 24 13 152

Belgaum 266 119 68 61 514

Deolali 328 114 64 58 564

Secunderabad 570 190 82 122 964

Pimpri 874 366 205 188 1633

Gwalior 141 72 16 20 249

Jhansi 250 74 70 41 435

Ahmednagar 348 159 82 58 647

Panitola 165 68 23 32 288

Binnaguri 154 53 20 13 240

Dimapur 205 70 47 27 349

Missamari 341 101 55 48 545

Bengdubi 361 186 101 88 736

Guwahati 297 109 50 58 514

Jorhat 48 15 10 02 75

Panagarh 146 18 20 24 208

Udhampur 75 03 08 0 86

Jammu 400 180 94 83 757

Karu 27 0 08 03 38

Kargil 64 15 07 08 94

Nowshera 286 83 55 58 482

Total 10577 3909 2209 1929 18624

21



Table 2. Elite cows at various Military Farms(2014-15)

S. Command/Farm NumberNo.

Southern

1 PIMPRI 642 BELGAUM 323 DEOLALI 194 AHMEDNAGAR 255 SECUNDRABAD 496 JHANSI 387 GWALIOR 16

Total 243

Western

1 AMBALA 962 JALANDHAR 383 FEROZPUR 274 PATHANKOT 335 DAGSHAI 136 YOL 08

Total 215

Central

1 MEERUT 1022 AGRA 403 BAREILLY 364 LUCKNOW 975 KANPUR 446 ALLAHABAD 467 JABALPUR 538 NAMKUM 089 MHOW 39

Total 465

Northern

1. JAMMU 572. UDHAMPUR 083. NOWSHERA 07

Total 72

Eastern

1. PANAGARH 122. GUWAHATI 473. BENGDUBI 414. PANITOLA 075. DIMAPUR 09

Total 116Grand Total 1111

Table 3. Farm wise receipt of Frieswal malecalves at BRU Meerut (2014-15)

S. Command/Farm NumberNo.

Southern

1 PIMPRI 112 AHMEDNAGAR 013 JHANSI 014 GWALIOR 01

Total 14

Western

1 AMBALA 142 JALANDHAR 033 PATHANKOT 01

Total 18

Central

1 MEERUT 272 AGRA 043 BAREILLY 014 LUCKNOW 095 KANPUR 026 NAMKUM 027. ALLAHABAD 018. JABALPUR 02

Total 48

Northern

1 JAMMU 13

Eastern

1 BENGDUBI 07

Grand Total 100

Multiplication and dissemination ofFrieswal germplasm

Under this project, a total of 9,79,975frozen semen doses of high genetic merit HFbulls were procured from USA, France,Canada, Hessarghatta, Salon and Rishikeshfor distribution to Military Farms for breedingthe lower crosses. Since inception of theproject a total of 34,07,000 frozen semen



22

doses of Frieswal bulls (upto 31st March2015) have so far been produced including3,37,485 doses in 2014-15. A total 67,323doses were distributed to Military Farms and71,868 doses were sold to para vets, StateAnimal Hubandry Departments, LivestockDevelopment Boards and State AgricultureUniversities during 2014-15 with a revenuegeneration of Rs. 10,05,732/-.

Performance evaluation of Frieswalanimals

The lactation records of 44,385 Frieswalcows sired by 165 bulls and maintained invarious agro-climatic regions over the periodof 24 years (1991 to 2014) were used forperformance analysis. The data wereclassified according to farm, parity, seasonand year of birth/calving. The seasons wereclassified as winter (December to March),summer (April to June), rainy (July toSeptember) and post monsoon (October andNovember). The data for production andreproduction traits were analyzed usingModel 1 and 2 of LSMLMW – PC Package(Harvey, 1990) using farm, parity, season andyear of calving as fixed effects and age atfirst calving as co-variable. Model used foranalysis of age at first calving included farm,year and season of birth as fixed effects.

Milk production performance

The overall means of 300 days milk yieldand total milk yield were 3284.78 and3320.47 kg, respectively. The peak yield ofthe Frieswal herd averaged 15.11 kg. Theeffects of farm, parity, season and year ofcalving and regression on AFC weresignificant on all the traits. Frieswal cows atMF Mhow produced the highest 300 daysmilk yield of 3879.60 Kg followed by Lucknow(3815.05 kg) and Allahabad (3750.28 kg).Frieswal cows at Kanpur had the highest totallactation milk yield of 4019.37 kg followed byAllahabad (3884.09 kg) and Mhow (3863.21kg). The peak yield was highest at MFAllahabad (17.96 kg) followed by Kanpur(16.79 kg) and Agra (16.75 kg). The Peakyield ranged from 11.69 kg at MF Panagarhto 17.96 at MF Allahabad. The variation inproduction performance among farms mightbe due to varying managemental and feedingpractices and location of farm in various agro-climatic zones. Season of calving also playeda significant role in production performance.The winter calved cows produced highest300 days milk yield of 3354.91 kg followedby post monsoon (3342.76 kg), summer(3226.53 kg) and rainy (3204.91 kg) calvers.Same trend was observed in total lactationmilk yield as well as peak yield. The totallactation milk yield and peak yield of animalscalved in winter was highest (3377.76 and15.81 kg) followed by post monsoon (3341.92and 15.41 kg) and summer (3319.30 and14.76 kg), respectively. The animals calvedduring the rainy season had the lowest totallactation milk yield and peak yield of 3242.92and 14.45 kg, respectively. There was lot offluctuation in total lactation milk yield duringvarious years and ranged from 3014 kg to3509.33 kg. Higher peak yields wereFrieswal bull

23



observed in animals which calved in the years2003 (16.10 kg) and 2001 (16.07 kg).

The average lactation length of theFrieswal cows was 326.56 days. The effectsof farm, parity, season and year of calving andregression of AFC were significant onlactation length. Lactation length was longestat MF Rajouri (353.07 days) followed by MFKaru (343.65 days). All the Military Farms hadlactation length higher than 300 days. Thecows calved during summer season had thelongest lactation length (335.09 days).

Reproductive performance

The overall mean of age at first calvingwas 973.25 days. The effects of farm, seasonand year of birth were significant on AFC.The animals at MF Dimapur (898.46 days)had shorter age at first calving followed byNamkum (912.24 days) and Lucknow(920.17 days). The longest AFC wasrecorded at MF Rajouri (1057.37 days)followed by Gwalior (1030.95 days). Largevariation in AFC reflects the scope for itsimprovement by improving the generalmanagement practices including feedingstandards, timely heat detection and artificialinsemination. Declining trend in AFC over theyears indicated a desirable improvement inthis direction.

Service period (SP), Dry period (DP) andCalving interval (CI) were also evaluated andthe average estimates were 145.47, 104.43and 424.29 days, respectively. These traitswere also significantly influenced by farm,parity, season, year of calving and regressionon AFC. The shortest service period wasobserved at MF Bengdubi (130.40 days)followed by Secunderabad (132.97 days) andJhansi (133.86 days). Longest service periodwas observed in cows at MF Rajouri (170.92days). Similar trend was observed for calvinginterval also, as expected. The shortestcalving interval was observed in cows at MFBengdubi (408.96 days) followed by thoseat Secunderabad (409.74 days) and Jhansi(412.57 days). The longest CI was noticed atMF Rajouri (447.63 days). MF Mhow had theshortest dry period (90.37 days) followed byMF Jabalpur (90.87 days) and Jhansi (91.82days). Longest DP was found at MF Gwalior(119.57 days). In general, service period andcalving interval had no definite trend over thelactations. Frieswal cows calved during postmonsoon season had the shortest SP(128.83 days) followed by those calved inrainy (140.24 days), winter (150.09 days)and summer (162.71 days) seasons.Seasonal variation in the other reproductivetraits also showed similar pattern and postmonsoon calvers had shortest DP and CI (97and 407.95 days) and summer calvers thelongest DP and CI (111.62 and 441.49 days).There was no definite trend in SP and CI overthe years. Longest SP was found during 1998(168.47 days) and shortest during 2013(91.52 days). The cows calved during 1999had longest (119.87 days) and thereafter adeclining trend in dry period was observed.There was a continuous decline in CI from1998 (447.15 days) to 2012 (402 days)reflecting the improvement made in thereproductive traits at Military Farms over the

Frieswal herd



24

years.

The sex ratio of Frieswal animals wasfound altered and the proportion of male birthwas 56.54 per cent. Chi square analysisrevealed no significant effect of month andyear of service and parity of the animals onthe sex ratio, while, farm effect wassignificant. Except MF Ranikhet (47.37%), allother farms had higher proportion of malebirths. The highest proportion of male birthswas found at MF Gwalior (70.54%). Amongthe 165 sires only 12.5% had proportion ofmale births lesser than 50.

Estimation of genetic parameters

The heritability estimates for AFC(0.052±0.010), TMY (0.054±0.009), MY300(0.0460±0.008) and PY (0.052±0.008) werevery low. SP, DP and CI also had lowheritability estimates of 0.009±0.004,0.004±0.005 and 0.008±0.004, respectively.All the traits, in general, had low standarderrors of their estimates. Low heritabilityestimates of production and reproductiontraits reflected that these traits could beimproved mainly by improving management/feeding practices at the Military Farms. Thetotal lactation milk yield had high positiviegenetic correlations with peak yield(0.922±0.022) and LL (0.635±0.085).

(B) GENETIC IMPROVEMENT OFCROSSBRED CATTLE UNDERFIELD CONDITIONS: Field ProgenyTesting of Frieswal Bulls

FPT Project was started in the 8th FiveYear Plan with the objective to undertakeprogeny testing of crossbred bulls on a largescale under the field conditions. Theprogramme is supported by AcademicInstitutions (SAUs), State Government andNon-Government Organizations. Thecrossbred cattle available in different agro-climatic region of the country are beingimproved through utilization of high qualitygermplasm of genetically superior breedingbulls. Presently the programme is operatingin collaboration with Kerala Veterinary andAnimal Sciences University, Thrissur, Kerala,(KVASU), Guru Angad Dev Veterinary &Animal Sciences University, Ludhiana,Punjab, (GADVASU), BAIF DevelopmentResearch Foundation, Uruli-Kanchan, Puneand G B Pant University of Agriculture &Technology, Pantnagar, Uttarakhand(GBPUA&T). Through the project intervention,the average first lactation 305 days milk yieldof the crossbred progenies in the adoptedvillages has increased by 37.9 % at KVASU,38.1 % at GADVASU and 11.7 % at BAIFunit. Also, the average age at first calving ofthe crossbred progenies has reduced by 11% at KVASU, 38.2 % at GADVASU and 24% at BAIF unit. The details on thecomparative performance of four units arepresented in Table-8.

Guru Angad Dev Veterinary & AnimalSciences University (GADVASU),Ludhiana, Punjab

The project envisioned to improvecrossbred cows through Field ProgenyVisit of GADVASU field unit by Director, ICAR-CIRC

25



Testing program in 86 adopted villages and30 artificial insemination (AI) centers inLudhiana district of Punjab. A total of 254crossbred bulls have so far been used intwelve different sets and 1,29,554inseminations were carried out, among them1,26,682 AIs were followed for pregnancydiagnosis and 55,481 pregnancies wereconfirmed since inception of the project. Theoverall conception rate was 43.8% based ontotal inseminations (Table-4). A total of 2,705daughters (184 from 1st, 154 from 2nd, 397from 3rd, 361 from 4th, 361 from 5th, 278 from6th set, 351 from 7th, 300 from 8th, 318 from9thsets and 01 from 10th set of bulls) from tensets of bulls have completed their firstlactation among the 16,185 female calvesborn so far. The average first lactation 305days milk yield of the daughters of first ninesets of bulls showed an increasing trend. Theaverage first lactation 305 days milk yieldsof the crossbred progenies in the adoptedvillages in the year 1993 was 2449.7 kg whichincreased to 3703.6 kg during the year 2014.The age at first calving showed positivedecreasing trend from 1st (1192 days) to 10th

set (736 days; partial records) with aremarkable reduction of more than 400 days.

The frozen semen of 11th and 12th set oftest bulls, belonging to GADVASU, Ludhiana,BAIF, Pune and Frieswal Project, Meerutwere used for performing 6,188 artificialinseminations in the adopted villages ofLudhiana district during the reporting period.The first lactation 305 days milk yield (kg) of361 daughters of fifth set, 278 daughters ofsixth set, 351 daughters of seventh set, 300daughters of eighth set and 318 daughtersof 9th set of test bulls ranged from 3331.2 to2437.8, 3474.7 to 2413.5, 3546.0 to 2808.3,3969.6 to 2635.9 and 4169.3 to 3373.3,respectively. The average first lactation 305days milk yields (kg) of the daughters of 5th

,6th ,7th , 8th , 9th and 10th set of bulls were2855.9, 3051.8, 3305.4, 3554.3, 3727.7 and

Table. 4. Information regarding different sets at GADVASU, Ludhiana since inception.

Set Date of Total bulls Total insemi- Total A.I.’s Pregnancies Conception Followed Female calves

No start used nations followed confirmed Rate % for calving Born ReachedAFC

I 01.04.95 18 7595 7355 3065 41.7 3000 855 227II 01.01.97 10 5150 4865 2132 43.8 2000 789 210III 01.01.99 23 18006 17159 8258 48.1 8000 1844 562IV 16.12.01 30 12548 11504 5720 49.7 5720 1368 490V 01.04.03 22 10409 10154 4362 43.0 4362 1497 478VI 01.02.05 25 8265 8105 3476 42.9 3476 1181 359VII 01.08.06 22 9710 9710 3999 41.1 3999 1120 448VIII 01.01.08 16 9611 9611 3898 40.6 3898 1186 461IX 01.07.09 24 14581 14581 5679 38.9 5679 1671 738X 01.03.11 21 12971 12971 5604 43.2 5604 2072 125XI 01.08.12 28 15662 15662 7008 44.7 6766 2492XII 01.02.14 15 5046 5005 2280 45.6 263 110Total 254 129554 126682 55481 43.8 52767 16185 4098

Crossbred cow at GADVASU field unit



26

2684.3 (partial records), respectively(Fig-1) and the corresponding averages forage at first calving were 1174.9, 1115.9,1082.2, 1014.3, 934.9 and 736.0 (partialrecords) days, respecively. The average fatpercentage in milk of the daughters of fifth,sixth, seventh, eighth, ninth and tenth set ofbulls was 3.7, 3.6, 3.6, 3.7, 3.7 and 3.7,respectively.

Socio-economic status of farmers underthe project

Crossbred cows maintained by thefarmers with own fodder production had

higher milk yield than the cows maintainedby farmers without fodder production.Commercial farmers had higher milk yield(3709.5 kg) than the non-commercial farmers(3127.8 kg). Availability of remunerative milkprices promoted the commercial farmers togive more attention to feeding andmanagement practices. Crossbred cowsmaintained by farmers having adequatefodder supply produced 3710.8 kg of milkduring first lactation against 3151.4 kg by thefarmers having little or no green fodder supply(Table-5). The maximum milk production of3850.1 kg was recorded by owners having

Fig. 1. Set wise progeny performance at GADVASU, Ludhiana

Fig. 2. Land holding of animal owners in the project

27



Fig. 3. Occupation of animal owners in the project

college level education. Educated ownershad more awareness on dairying andscientific farm practices for profitable milkproduction.

It was also observed that animals of thefarmers having more than 10 acres of landholding produced maximum average milk(4654.5 kg) (Fig-2). About 10.3% of the ownershad dairying as their whole time occupation.The average milk production of the cows rearedby such farmers was 4366.2 kg which wassignificantly higher than the animals of owners

having occupations of agricultural labour andbusiness (Fig-3). The farmers having herd sizeof more than ten animals had the maximumlactation milk yield (3998.4 kg).

The milk production of the cows rearedby the agricultural farmers (3634.5 kg) wasalso higher than agricultural labour andbusiness. This could be due to the fact that theagricultural farmers paid more attention towardsdairy farming along with agriculture farming andprovided good quality feed, fodder, health careand management to the animals.

Table. 5. Production performance of animals according to different categories of farmers

Category No. Percentage 1st lact. 305-days milk yield (kg.)

Overall mean 390 100 3703.6±31.3

Feeding System

1. With fodder 385 98.7 3710.8±31.52. Without fodder 5 1.3 3151.4±125.1

Type of farmer

1. Commercial 386 99.0 3709.5±31.52. Non-commercial 4 1.0 3127.8±122.0

Education

1. Illiterate 5 1.3 3444.0±261.82. Up to primary 51 13.1 3689.0±64.73 Up to matric 291 74.6 3712.2±37.1



28

Category No. Percentage 1st lact. 305-days milk yield (kg.)

4. Up to secondary 30 7.7 3625.3±135.45. College level 13 3.3 3850.1±150.7

Herd size

1. < 3 51 13.1 3512.7±60.52. 4 to 5 91 23.3 3541.8±48.43. 6 to 10 164 42.1 3701.8±50.04. > 10 84 21.5 3998.4±77.4

Land holding

1. Landless 5 1.3 3151.4±125.12. Less than 1 acre 8 2.1 3640.8±165.13. 1 to 2 acres 30 7.7 3537.5±81.74. 2 to 5 acres 133 34.1 3676.9±49.35. 5 to 10 acres 201 51.5 3700.8±43.96. > 10 acres 13 3.3 4654.5±209.2

Occupation of owner

1. Agric. Farmer 336 86.2 3634.5±29.02. Agric. labour 6 1.5 3600.2±213.03. Service 6 1.5 3344.2±169.84. Business 2 0.5 3448.3±32.45. Dairying 40 10.3 4366.2±141.9

Kerala Veterinary and Animal SciencesUniversity (KVASU), Thrissur, Kerala

The progenies born to test bulls performedbetter than their contemporaries throughoutthe period of implementation of the project.Field progeny testing program at this unit isrunning through seven field units and sixartificial insemination centers along with threelivestock farms of KVASU. First set of bulls

were inducted in 1992 and at present 13th

set of bulls is in progress. Two hundred thirtyeight bulls have so far been inducted in 13different sets. A total of 1,03,318 artificialinseminations were carried out, 59,034inseminations were followed for pregnancydiagnosis and 24,043 pregnancies wereconfirmed since inception with an overallconception rate of 38.3 % (Table-6). A totalof 1,445 daughters from first ten sets havecompleted their first lactation. The milk yieldshowed almost increasing trend among theprogenies of different sets (Fig-4) and theaverage 305 days milk yield was 1958 kg(1st set) and 2623 kg (10th set). The averageage at first calving of progenies of the testbulls and contemporaries calved during reportperiod was 1009.52 and 1250.10 days,respectively. The progeny born from test bullsof the scheme produced 500 liters more milkper lactation than the other cows in the areaA crossbred cow with owner at KVASU unit

29



Table. 6. Information regarding different sets at KVASU, Thrissur since inception.

Set Date of Total bulls Total insemi- Total A.I.’s Pregnancies Conception Followed Females calves

No start used nations followed confirmed Rate % for calving Born ReachedAFC

1 01/01/1992 12 23351 6722 2420 36.0 1902 956 319

2 01/04/1994 11 12817 4800 1680 35.0 1300 603 240

3 01/09/1995 11 9331 3942 1324 33.6 1065 757 89

4 01/11/1998 15 11750 3753 1501 39.9 1489 676 178

5 09/11/2001 19 3437 2361 1136 34.8 847 401 139

6 24/06/2003 20 8173 7683 2582 33.6 1689 746 216

7 16/03/2005 24 5759 5211 2281 43.7 1298 597 180

8 30/08/2006 22 5703 5514 2472 44.8 1538 768 160

9 05/02/2008 16 3393 3131 1181 37.7 801 394 81

10 01/07/2009 24 5781 5612 2124 37.8 1324 664 115

11 25/05/2011 21 4820 4401 2006 45.6 1187 619 16

12 10/10/2012 28 5972 3920 2311 39.3 1148 575 0

13 14/03/2014 15 3031 1984 1025 36.2 4 0 0

Total 238 103318 59034 24043 38.3 15592 7756 1733

Fig. 4. Set wise progeny performance (milk yield and age at first calving) at KVASU, Thrissur Unit

and farmers got extra income of Rs.16,000/- per cow per year. The project hassubstantially contributed by the production ofmore than 10,000 female progenies of highgenetic merit. The average fat percentage inthe milk of progenies of these sets was 3.7%.

A total of 28 bulls from 12th set were usedfor insemination and 5,972 artificialinseminations were carried out with anaverage conception rate of 39.3%. Out of total2,311 progenies confirmed, 575 were femaleprogenies. A total of 15 bulls from 13th setwere used for insemination and 3,031



30

artificial inseminations were carried out withaverage conception rate of 36.2% and a totalof 1,025 pregnancies were confirmed. Duringthe reporting period, average first lactation305 days milk yield (kg) and age at first calving(days) of the progenies of 8th, 9th and 10th setof bulls were 2659.5 & 1095.9, 2701 & 1112and 2623 & 1021, respectively. The loss ofdata during the report period was 35.8% for10th set and 27% for 11th set and 20.7% for12th set of bulls.


Analysis of socio economic status of thefarmers of the project gives an idea aboutthe status of the farmers of the state involvedin dairying. Around 75% of the animal owners

were agriculture farmers (Fig-5). They keptthe animals as a subsidiary enterprise ofagriculture. Agriculture labours accounted for19% and the other occupation for the animalowners were negligible. This indicates theclose linkage of agriculture and animalhusbandry practices in the project area.

The educational status of animal owners wasalso studied. The high literacy rate of the state isreflected in the educational status of owners. Morethan 47% of the animal owners had educationalqualification of higher secondary / college and 32%were graduate (Fig-6).

There was no landless owner of theanimals. Around 70% of animal owners hadless than 1 acre of land (Table-7). The farmerswith more than one hectare of land were 4.3%.

Fig. 5. Occupation of animal owners in the project

Fig. 6. Education status of animal owners in the project

31



Nearly 68% of the owners of animals had herdstrength of 3 or less. The herd size of morethan 10 animals was observed only in 2.43%owners. An analysis of different groups ofcattle in the progeny testing herd of KVASU,Trissur showed that cows and calvesaccounted for around 40% each of thepopulation whereas the dry cows and heifers

accounted for approximately 6% and 14%,respectively. This shows that the replacementstock is very low in the population. There is asignificant reduction in population of thesegroups compared to last year. There was ageneral scarcity for green grasses andtherefore paddy straw was used as mainroughage for cattle. Rest of the energy

Table. 7. Production performance of animals of different categories of farmers at KVASU, Thrissur .

Category No. Percentage Average 1st lact.305 days milk yield (kg)

Average No.

Overall mean 369 2555.46 48

Feeding system

1. With fodder 69 18.70 2522.7 122. Without fodder 300 81.30 2566.4 36

Education

1. Illiterate 0 0 0 02. Up to primary 60 16.26 2532.8 83. Up to metric 120 32.52 2532.8 184. Up to secondary 172 46.61 2571.8 215. College level 17 4.61 2802.5 1

Herd size

1. <3 251 68.02 2470.0 242. 4 to 5 72 19.51 2579.3 153. 6 to 10 37 10.03 2743.6 94. >10 9 2.44 0 0

Land holding

1. Landless 0 0 0 02. Below 10 cents 52 14.09 2705.8 63. 10 -49 cents 115 31.17 2538.5 124. 50-99 cents 89 24.12 2605.0 135. 1 acre-2 acres 71 19.24 2419.5 126. 2 -3 acres 26 7.05 2635.8 37. 3 -4 acres 10 2.71 2578.8 28. >4 acres 6 1.62 0 0

Occupation of farmer

1. Agric. Farmer 276 74.80 2579.7 392. Agric. Labour 69 18.70 2511.8 73. Govt Job 13 3.52 2235.0 24. Pvt. Job 6 1.63 0 05. Business 5 1.36 0 0



32

required was met from concentrate feeding.Feeding pattern of the animals under theproject was observed as partial grazing andstall feeding was the most common practicein the project area. Limited land availabilityfor grazing, forced the keepers to opt for stallfeeding with roughages by cut and carrysystem.

BAIF Research DevelopmentFoundation, Uruli-Kanchan, Pune,Maharashtra

The crossbred population in the region isdominated by various grades of HolsteinFriesian. Till the reporting period, semendoses from 230 crossbred bulls were usedfor breeding the farmers’ animals. The 11th

batch of bulls started in March 2014 and thisbatch comprised of only Frieswal bulls. Tillthe reporting period, 10 bull batchescompleted test inseminations and 11th bullbatch was in progress for test inseminations.From these bull batches a total of 94,423inseminations were performed, 87,232inseminations (92.38%) were followed for

Table. 8. Comparative performance of 4 different units of FPT project during the reporting period(1.1.2014 to 31.12.2014)

Particulars KVASU, GADVASU, BAIF, GBPUAT,Thrissur Ludhiana Pune Pantnagar

Total Artificial inseminations 4408 6188 5771 3310

Pregnancies confirmed 1571 4520 2645 1352

Conception rate (%) 37 46.4 43.04 56.7

Total calving 764 3809 1869 941

Female calves born 382 1770 900 447

Female calves reached AFC 92 474 243 121

Female calves completed 1st lactation 64 390 157 87

Average 305 days milk yield(kg) 2678.3 3703.6 3206.64 2587.3

Average AFC(days) 1133.9 1036.6 956.88 1044

Total loss of data (%) 17.04 33.7 35.39 10.99

A crossbred calf with dam at BAIF unit

pregnancy diagnosis and 39,495pregnancies were confirmed with an averageconception rate of 45.28 per cent (Table-9).From these pregnant animals 25,101 werefollowed for progeny birth and 11,576 femaleprogenies were born till the reporting period.A total of 4,126 progenies reached to age atfirst calving (AFC). Total seven sets of bullbatches completed progeny performancewhile progenies from bull batches introducedduring August 2009 (8th set) and April 2011(9th set) are under milk recording. The 10th

bull batch is in calving stage and 11th bullbatch is under insemination and pregnancydiagnosis stage.

33



During the reporting period a total of3,506 artificial inseminations were performedfrom the bulls of 11th batch and 2,260inseminations were followed for pregnancydiagnosis. A total of 918 pregnancies wereconfirmed with an average conception rateof 40.62 per cent. The first lactation 305 daysmilk yield (kg) of 248 daughters of 6th set,192 daughters of 7th set, 189 daughters of

8th set and 36 daughters of 9th set of testbulls were estimated as 3008.7, 3094.1,3181.4 and 2901.6, respectively (Fig-7) andthe corresponding average AFC was 32.8,32.5, 30.1, and 25.0 months. The averagefat percentage of milk of the daughters of 6th,7th, 8th and 9th set of bulls was 3.6, 3.6, 3.58and 3.59, respectively.

Table. 9. Information regarding different sets of bulls at BAIF, Pune since inception.

Set Bull batch Total bulls Total A.I. Total A.I.’s Pregnancies Conception Followed Females calves

No starting date used done followed confirmed Rate % for calving Born ReachedAFC

I July1995 20 16118 15063 7001 46.48% 4868 2344 1563

II Feb.1998 19 21321 17239 7673 44.51% 3815 1756 514

III July 2001 20 7461 7380 3398 46.04% 2626 1201 364

IV July 2003 20 5249 5162 2162 41.88% 1493 731 289

V Feb. 2005 25 6806 6638 2989 45.03% 1969 856 394

VI Sept. 2006 22 6533 6327 2899 45.82% 1993 885 371

VII Feb. 2008 16 4902 4902 2169 44.25% 1561 733 313

VIII Aug. 2009 24 6893 6867 2987 43.50% 1997 878 267

IX April 2011 21 6364 6364 3109 48.85% 2270 1010 51

X Aug. 2012 28 9270 9030 4190 46.40% 2509 1182 00

XI Mar-14 15 3506 2260 918 40.62% 0 0 0

Total 230 94,423 87,232 39,495 45.28% 25,101 11,576 4,126

Fig. 7. Set wise progeny performance at BAIF, Pune



34


Socio-economic status of farmers underthe project revealed that out of 1,480 farmers,40.41 per cent had education up to primarylevel, 37.09 per cent upto secondary, 8.51per cent higher secondary and 4.59 per centgraduate and above (Table-10).

The proportion of illiterate cow ownerswas 9.39 per cent (Fig-8). The average herdsize in field progeny testing area was 6.04.In this, more than 3/4th share was of Holstein-Friesian crossbred population (88.77%)followed by Jersey crossbred (9.63%).Majority of herd owners (48.79%) hadpermanent or semi-permanent cattle sheds

where as, other owners had either temporary(20.13%) or thatched (31.08%) housing. Noneof the farmer had kept animals without shelter.Most ot the cattle owners (90.95%) wereagriculturists and almost all (95.74%) ownedland. Nearly 59 per cent farmers had land upto 5 acres and the percentage of farmershaving land more than 10 acres was 10.74.Among landholders, more than 2/3rd (69.53%)cattle owners were cultivating different foddercrops (Fig-9).

The fodder crops generally grown andavailable to animals were Jowar and Bajrastraw, sugarcane tops, lucerne and maize. Inconcentrate, farmers fed ready-made feedpurchased from market.

Table. 10. Socio-economic status of Farmers

A. Education

Education Illiterate Primary Secondary Higher Graduate & TotalLevel secondary above

No. of cow owner 139 (9.39) 598 (40.41) 549 (37.09) 126 (8.51) 68 (4.59) 1480 (100)

B. Land holdings

Frequency of farmers Land holding (Acres) Total

0 Up to 5 5 To 10 Above 10

According to total land holding 63 (4.26) 875 (59.12) 383 (25.88) 159 (10.74) 1480 (100)

According to land under fodder 451 (30.47) 955 (64.53) 61 (4.12) 13 (0.88) 1480 (100)crop

C. Main Occupation

Occupation Agriculture Dairy Service Other Agriculture Business TotalType labour

No. of Cow Owners 1346 (90.95) 50 (3.38) 38 (2.57) 18 (1.22) 16 (1.08) 12 (0.81) 1480 (100)

D. Cattle housing

Housing Type Permanent Semi-permanent Temporary Thatched Total

No. of Herds 264 (17.84) 458 (30.95) 298 (20.13) 460 (31.08) 1480 (100)

35



Fig. 8. Education status of farmers participating inthe project

Fig. 9. Land holding wise frequency of farmers

GB Pant University of Agriculture &Technology (GBPUA&T), Pantnagar,Uttarakhand

The unit started in the year 2009 andcovers 7 A.I. centers. A total of 3,000 frozensemen doses of 10 bulls of 1st set (duringJanuary 2010), 2906 doses of 6 bulls of 2nd

set (March 2011), 4410 doses of 9 bulls of3rd set (July 2012 & October 2013) wereutilized for test inseminations. A sum of 7350semen doses of 15 bulls of 4th set (February2014) were also provided to carry out testinseminations in crossbred cows. A total of40 bulls have so far been introduced in 4different sets and 9,496 inseminations havebeen done from which 1,492 femaleprogenies were born so far of which 192

progenies have reached age at first calving(Table-11).

During the year 2014, a total of 3,310 AIwere carried out, 2,385 were followed forpregnancy diagnosis and 1,352 wereconfirmed pregnant with a conception rate of56.7%. Two heifer shows in field and oneinseminator cum data recorder training atUniversity were conducted. A total of 250farmers and 10 inseminators cum datarecorders participated in training.

Socio-economic status

Regarding education level 43.7 per centfarmers had education upto secondary level,12.6 per cent upto primary level, 17 per centhigher secondary and 12 per cent graduate,whereas 11 percent farmers were illiterate.Nearly 21 per cent farmers had land up to 5-10 acres and the percentage of farmershaving land more than 10 acres was 15.5(Table-13 and Fig-10), whereas, 18 percentfarmers were landless. Sixty one (61) percentanimals were kept in attached housingsystem whereas, 39 per cent were kept inseparate sheds. All the animals weremaintained under stall feeding system.Frieswal Heifer Show at GBPUA&T, Pantnagar



36

Table. 11. Information regarding different sets at GBPUA&T, Pantnagar since inception

Set Date of Total bull Total Total A.I. Pregnancies Followed for Female calves

No. start used Insemination followed confirmed calving Born Reached AFC

I 20.01.10 10 1784 1750 1030 997 340 151

II 16.03.11 6 2303 2258 1546 1529 542 41

III 25.07.12 9 2473 2243 1405 1382 573 -

IV 05.02.14 15 2936 2011 1135 1128 37

Total 40 9496 8262 5116 5036 1492 192

Table. 12. Set wise progeny performance of GBPUA&T, Pantnagar (01.01.2014-31.12.2014)

Set No. Progeny Progeny Average Average Average Fat % (Month)

and bull reached completed AFC(Month) Ist lactat.ion 2nd 5th 8th

name AFC Ist lactation milk yield

Set - I 80 65 38.2 2598.4 3.4 3.9 4.1

Set –II 41 2 27.3 2116.7 3.9 3.9 4.0

Table. 13. Education, occupation and land holding of animal owners

EDUCATION Illiterate Primary Secondary HSC Graduate PG Diploma11.0 12.6 43.7 17.0 12.0 2.0 2.0

LAND HOLDING Landless < 1 acre 1-2 acre 2-5 acre 5-10 acre > 10 acre18.0 12.7 13.5 19.3 20.6 15.5

OCCUPATION Farmer Labour Service Business Dairy Other66.0 16.5 14.7 2.1 0.3 0.15

HOUSING Attached Separate Perm. Semi perm. Temp. Thatch61.4 38.6 56.6 29.1 12.8 1.4

FEEDING Stall Grazing Both100 0 0

Fig. 10. Land holding size of animal owners in the project

37



(C) CONSERVATION AND GENETICIMPROVEMENT OF INDIGENOUSCATTLE BREEDS: IndigenousBreeds Project

Genetic studies on performance ofimportant indigenous breeds of cattleand their improvement through selection

The primary objective of the project is toconserve and bring out genetic improvementin some of the important indigenous cattlebreeds of our country. The project is beingimplemented in the home tract of indigenousbreeds maintained under farm and fieldconditions. Presently three important breedsviz., Gir, Kankrej and Sahiwal are included inthe project. The project envisagesestablishment of germplasm (G.P.) and datarecording units (D.R.) for each breed incollaboration with the State AgriculturalUniversities and ICAR institutes. The youngbulls born out of nominated mating of elitecows at the germplasm centre are to beprogeny tested using animal and farmfacilities existing at the Data Recording Units/associated herds.

About 750 breedable females areidentified at 4-5 data recording units (herds)and each bull is to be mated with 60 to 70females in order to provide first lactation milkproduction records of at least 20 progeniesper bull. The information on growth,reproduction, milk production and survivabilitywill be recorded for performance evaluation,selection and genetic improvement ofrecommended indigenous cattle breeds.

GIR BREED

The GP unit of Gir cattle is located at CattleBreeding Farm, Junagadh AgriculturalUniversity, Junagadh (Gujarat) and the DRunits are located at 14 farmers herds and

three organised associated herds. So far10,673 breedable females have beenidentified under the project.

A herd of Gir cattle

Herd strength

The herd strength of GP unit as on 31st

December 2014 was 134 which included 94females and 40 males. The breedablefemales aged above 2.5 years were 67(Table-16) while the number of cows in milkwas 29. The number of bulls available forbreeding was twelve. The herd strength ofCBF, Junagadh DR unit as on 31st December2014 was 391, out of which 289 were femalesand 102 males. The unit also possessed 06breeding bulls and 50 young bulls above oneyear. A total of 10,673 breedable femaleswere identified under the project at organizedfarms (388) as well as field units (10,285).

Bulls inducted and frozen semen dosesproduced

In the project, 15 Gir bulls in two sets (6 inset I and 9 in set II) have been inducted sofar. The opening balance of semen doses was14,676 (8,937 and 5,739 for bulls of set I andII, respectively). During the year 2014, 10,097semen doses were produced from bulls ofset–I (2,003) and set-II (8,094). A total of 3,928doses were utilized for insemination (552 ofset I and 3,376 of set II), 758 doses were sold



38

(213 of set I and 545 of set II) and 20,087doses were available as on 31st December2014 for future use. The details are presentedin Table-14.

GP and DR unit of CBF, Junagadh arepresented in Table-16 & 17.

Germplasm unit

The overall average first lactation milkyield was 2731.90 ± 437.60 kg while averagemilk yield for all lactations was 2572.50 ±264.20 kg. The average lactation length andpeak yield were 374.30 ± 20.60 days and13.90 ± 0.60 kg, respectively. The overall ageat first calving, service period, dry period, firstlactation length and calving interval were1284.50 ± 31.30, 139.10 ± 9.30, 200.20 ±33.70, 408 and 552.00± 46.80 days,respectively. The wet and dry averages of theGP unit were 7.40 ± 0.60 and 4.70 ± 0.50 kg,respectively.

Data recording unit

The average age at first calving of Gircattle maintained under DR unit of CBF,Junagadh was 1422.70 ± 27.30 days whileaverage estimates for service period, dryperiod and calving interval were 141.20 ±9.30, 272.70 ± 35.80 and 509.30 ± 26.30days, respectively. The averages for first andall lactation milk yields were 2200.90 ± 651.20and 2006.60 ± 225.00 kg, respectively withan average lactation length of 324.80 ± 15.90days. The average lactation peak yield was12.20 ± 0.70 kg while the wet and dryaverages were 6.0 ± 0.60 and 3.9 ± 0.40 kg,respectively. In general, the performance ofGP unit was better than DR unit indicatingthe superiority of elite females maintained inGP unit.

KANKREJ BREED

The GP unit of Kankrej cattle is located atLivestock Research Station,Sardarkrushinagar Dantiwada AgriculturalUniversity, Sardarkrushinagar, Gujarat and

A Gir bull from the set

Inseminations carried out, conceptionrate and daughters born

The details of inseminations carried out,conception rate and daughters born arepresented in Table-15. During the year 2014,a total of 2929 animals were inseminated andoverall conception rate was 46.12 per cent.The conception rate of set-II bulls (46.28%)was slightly higher than set I bulls (45.25%).The number of inseminations carried outsince inception for set-I and set-II were 11,168and 4,376 resulting in a conception rate of50.64 and 41.06 per cent, respectively.During the year 2014 a total of 587 daughterswere born which resulted in to a total of 3346daughters born since inception. The numberof normal male and female calvings in GPand DR units of CBF, Junagadh was 52 and43, respectively with a male : female ratio of54.74: 45.26. The percentage of abnormalcalvings was 5.94.

Productive and reproductiveperformance

The details of productive and reproductiveperformance of Gir cattle maintained under

39



five DR units consisting of organized farmsand farmers' herds have been identified underthe project.

Herd Strength

The herd strength of GP unit as on 31st

December, 2014 was 141 which included 96females and 45 males. The total number ofbreedable females above two years was 67and the number of animals in milking was 24.The unit also maintained 20 Kankrej bulls forbreeding purpose. The DR unit of LRS,Dantiwada had the herd strength of 124animals. The number of female and maleanimals was 99 and 25, respectively. The unitmaintained 58 adult breedable females ofwhich 28 were in milking. A total of 3,058breedable females were identified under theproject at organized farms and field units.

insemination, 2,410 doses were sold and6,262 doses were discarded resulting in abalance of 80,390 doses of frozen semen forbreeding.


During the reporting period 2,286 animalswere inseminated, 893 animals wereconfirmed for pregnancy and 263 daughterswere born. The conception rate during theyear 2014 was 39.06 per cent against overallconception rate of 46.45 per cent for two setof bulls. The number of daughters born forthe first and second set of bulls was 392 and475, respectively resulting in 867 Kankrejdaughters born since inception of the project.The number of normal male and femalecalving in the GP and DR unit of LRS,Sardarkrushinagar during 2014 was 33 and44, respectively with a male female ratio of42.86: 57.14 and 3 percent abnormal calvingwere also observed.


The details of productive and reproductiveperformance of Kankrej cattle maintainedunder GP and DR unit of LRS,Sardarkrushinagar are presented in Tables-16 & 17.

Germplasm unit

The overall averages for age at firstcalving, first service period, first dry periodand calving interval were 1398.75 ± 80.40,187.00 ± 75.99, 299.25 ± 56.17 and 431.35± 21.42 days, respectively while averageestimates for first lactation milk yield, alllactation milk yield and first peak yield were2136.04 ± 195.61, 2760.71 ± 134.67 and10.59 ± 0.33 kg, respectively. The average

Kankraj calves


Two sets of Kankrej bulls have beeninducted so far, the first set consisted of eightbulls from Banas Dairy while the second setconsisted of nine bulls. The opening balanceof semen doses as on 01st January 2014 was66,623 and during the year 2014, a total of33,670 doses of frozen semen wereproduced from 13 bulls. A total of 3,975 dosesof second set of bulls were utilized for



40

first lactation milk yield was lower than alllactation milk yield indicating improvement inmilk production in subsequent lactations. Theaverage first lactation length was 288.20 ±15.96 days while the wet and dry averageswere 9.14 and 6.86 kg, respectively.

Herd strength

During the year 2014, a total of 389Sahiwal animals were maintained at the GPunit. The number of breedable females andmales above 2 years was 214 and 31,respectively. The total number of breedablefemales registered in different DR units was599 (271 in GLF-I, Hissar, 74 in GADVASU,77 in Pantnagar, 81 in Anjora, Durg and 96 inGaushala, Bhiwani).

A Kankrej cow at farmer door

Data recording unit

The Kankrej cows maintained in the DRunit produced an overall average first and alllactation milk yields of 2101.66 ± 165.35 and2254.88 ± 102.26 kg, respectively. The firstlactation length was 314.50 ± 18.21 days.The average estimates for reproductive traitsviz., age at first calving, first service period,first dry period and calving interval were1329.75 ± 40.25, 204.21 ± 23.08, 145.33 ±34.91 and 461.07 ± 22.59 days, respectively.The first lactation peak yield was 10.72 ± 0.47kg while the wet and dry averages were 7.75and 4.82 kg, respectively.

SAHIWAL BREED

The GP unit of Sahiwal breed is locatedat National Dairy Research Institute, Karnaland five DR units are located at i) GovernmentLivestock Farm-I, Hissar, ii) GADVASU,Ludhiana, iii) Shri Gaushala Trust, Bhiwani,iv) G.B. Pant University of Agriculture andTechnology, Pantnagar, Uttarkhand and v)Cattle Breeding Farm, Anjora, Durg,Chhattisgarh.

Sahilwal cow with calf


Fifteen Sahiwal bulls in two sets havebeen inducted in the project for progenytesting. The first set comprised of eight bullswhile in the second set seven bulls wereinducted. The opening balance of frozensemen doses as on 01st January 2014 was49,236 and during the year 23,840 doseswere produced. Since inception of theproject, a total of 73,076 doses wereproduced and 13,262 were utilized (5,061doses were sold to the stakeholders and8,201 doses were used for insemination) andas on 31stDecember 2014, a total of 59,814doses were in stock for future use. During thereporting period 2,430 and 21,410 doses ofsemen were produced from the first andsecond set of bulls and 2,362 and 10,900doses were utilized so far resulting in a

41




The details of productive and reproductiveperformance of Sahiwal cattle maintainedunder GP and different DR units arepresented in Table-17.

Germplasm unit

The overall average estimates for age atfirst calving, first service period, first dryperiod and calving interval of Sahiwal cowswere 1238.22 ± 27.55, 138.75 ± 24.48,123.13± 33.27and 425.38± 25.41 days,respectively while average estimates for firstlactation 305-day milk yield, total firstlactation milk yield and first peak yield were2054.92 ± 229.49, 2437.79 ± 379.27 and11.41 ± 0.69 kg, respectively. The averagefirst lactation length of the herd was 316.53 ±31.69 days while the wet and dry averageswere 7.17 and 3.47 kg, respectively.

Data recording units

Among the different DR units, the Sahiwalherd maintained at Pantnagar showed lowestage at first calving (1223.00 ± 33.84) dayswhile the highest age at first calving (1422.00± 37.96 days) was observed in GADVASU,Ludhiana unit. The first service period waslowest (101.20 ± 3.30 days) in GLF-I, Hissarfollowed by Pantnagar (140.13 ± 5.87 days)and GADVASU, Ludhiana (150.66 ± 35.84days). The first dry period was 145.00 ±12.00, 154.27 ± 19.23 and 175.33 ± 83.52days in GLF-1, Hissar, Pantnagar andGADVASU, Ludhiana units, respectively. Thehighest first lactation milk yield of 2156.00 ±106.00 kg was recorded in GLF, Hissarfollowed by Pantnagar (1608.08 ± 25.83 kg)and Ludhiana, GADVASU (1171.95 kg)while the all lactation milk yield estimates forthe above three units were 2249.00 ± 49.00,

balance of 25,409 and 34,405 doses for firstand second set, respectively.


During the reporting period 1,406inseminations (327 in NDRI, Karnal, 381 inGLF-I, Hissar, 88 in GADVASU, Ludhiana,399 in Pantnagar, 200 in Bhiwani and 11 inDurg unit) were carried out amounting to atotal insemination of 5,276 since inceptionof the project. During the year 2014, theconception rate was estimated as 32.56 and41.45 per cent for the first and second set ofbulls, respectively with an overall conceptionrate of 41.18 per cent. The conception ratesince inception was 36.69 and 40.33 for thefirst and second set of bulls, respectivelyamounting to an overall conception ate of37.85 per cent. So far 681 Sahiwal daughterswere born under the project. During the reportperiod, 184 (88 and 96 for first and secondset of bulls, respectively) daughters were bornand the number of daughters born in NDRIKarnal, GLF-I Hissar, GADVASU Ludhiana,Pantnagar and Bhiwani unit were 54, 63, 17,33 and 17, respectively. The number of normalmale and female calving in the GP unit, NDRI,Karnal during the year 2014 was 57 and 70,respectively with a male female ratio of44.88: 55.12. No abnormal calving wasobserved.

Sahiwal cow with young calf



42

Tab

le. 1

4. B

reed

wis

e se

men

do

ses

colle

cted

an

d u

tiliz

ed

Bre

edS

et N

o.

No

. of

Sem

en d

ose

sTo

tal

Do

ses

uti

lized

Bal

ance

as

bulls

on

31-

12-2

014

ind

uct

ed

Bal

ance

as

Pro

du

ced

Uti

lized

fo

rS

old

Dis

card

ed/

Tota

lo

n 3

1-12

-201

3d

uri

ng

bre

edin

g /

Oth

er20

14su

pp

lied

Sah

iwal

I8

2534

124

3027

771

177

2185

-23

6225

409

II7

2389

521

410

4530

580

2428

76-

1090

034

405

Ove

rall

1549

236

2384

073

076

8201

5061

-13

262

5981

4

Gir

I6

8937

2003

1094

055

221

3-

765

1017

5II

957

3980

9413

833

3376

545

-39

2199

12O

vera

ll15

1467

610

097

2477

339

2875

8-

4686

2008

7

Kan

krej

*I

966

623

3367

010

0293

1123

124

1062

6219

903

8039

0

Tota

l39

1305

3567

607

1981

4223

360

8229

6262

3785

116

0291

*Sem

en d

oses

of f

irst s

et b

ulls

wer

e pr

ocur

ed fr

om B

anas

dai

ry fa

rm

Tab

le. 1

5. B

reed

wis

e d

etai

ls o

f in

sem

inat

ion

car

ried

ou

t, c

on

cep

tio

n a

nd

dau

gh

ter

bo

rn(S

ahiw

al D

R u

nit

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ud

ed)

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edS

et N

o.

No

. of

AI d

on

eC

on

cep

tio

nD

aug

hte

rs b

orn

bulls

Up

toD

urin

gTo

tal

Up

toD

urin

gTo

tal

Up

toD

urin

gTo

tal

ind

uct

edD

ecem

ber

2014

Dec

emb

er20

14D

ecem

ber

2014

2013

2013

2013

Sah

iwal

I8

3557

4336

0013

07 (3

6.74

)14

(32.

56)

1321

(36.

69)

497

8858

5II

731

313

6316

7611

1 (3

5.46

)56

5 (4

1.45

)67

6 (4

0.33

)0

9696

Ove

rall

1538

7014

0652

7614

18 (3

6.64

)57

9 (4

1.18

)19

97 (3

7.85

)49

718

468

1

Gir

I6

1071

545

311

168

5450

(50.

86)

205

(45.

25)

5655

(50.

64)

2692

133

2825

II9

1900

2476

4376

651

(34.

26)

1146

(46.

28)

1797

(41.

06)

6745

452

1O

vera

ll15

1261

529

2915

544

6101

(48.

36)

1351

(46.

12)

7452

(47.

94)

2759

587

3346

Kan

krej

I8

2180

021

8011

29 (5

1.84

)9

1138

(52.

25)

381

1139

2II

923

2022

8646

0611

29 (4

8.66

)88

4 (3

8.67

)20

13 (4

3.70

)22

325

247

5O

vera

ll17

4500

2286

6786

2258

(50.

20)

893

(39.

06)

3151

(46.

45)

604

263

867

Fig

ures

in p

aren

thes

es in

dica

tes

the

conc

eptio

n ra

te in

per

cent

age

43



Table. 16. Number of breedable females registered and number of calves born in Germplasm units

Breed No. of breedable females registered Male calves Female calves

Sahiwal 214 70 57Gir 67 17 13Kankrej 67 14 19

Table. 17. Productive and reproductive performance of Indigenous cattle breeds

Gir Kankrej Sahiwal

GP unit DR unit GP unit DR unit GP unit DR units

Traits CBF, JAU, CBF, LRS, LRS, NDRI, GADVASU, GLF-I, GBPUA&T,Junagadh Junagadh Sardarkrushi Sardarkrushi Karnal Ludhiana Hissar Pantnagar

nagar nagar

Age at first calving 1284.50 ± 1422.70 ± 1398.75 ± 1329.75 ± 1238.22 ± 1422.00 ± 1378.00 ± 1223.00 ±(days) 31.30 (06) 27.30 (31) 80.40 (08) 40.25 (16) 27.55 (45) 37.96 (07) 25.00 (35) 33.84 (46)

(Partial data)First lactation 2731.90 ± 2200.90 ± 2136.04 ± 2101.66 ± 2437.79 ± 1171.95 2156.00 ± 1608.08 ±milk yield (kg) 437.6 (09) 651.20 (06) 195.61 (08) 165.35 (16) 379.27 (17) (02) 106.00 (29) 25.83 (33)Lactation milk 2572.50 ± 2006.60 ± 2760.71 ± 2254.88 ± - 1894.40 ± 2249.00 ± 1647.22 ±yield (kg) 264.20 (24) 225.00 (28) 134.67 (38) 102.26 (41) 128.98 (25) 49.00 (145) 40.44 (155)First lactation 408 324.80 ± 288.20 ± 314.50 ± 316.53 ± 230.00 326.00 ± 296.09 ±length (Days) 15.90 (28) 15.96 (08) 18.21 (16) 31.69 (17) (02) 10.00 (29) 5.20 (33)First peak yield 13.90*± 12.20 ± 10.59 ± 10.72 ± 11.41 ± 11.22 ± 9.33 ± 9.7 ±(kg) 0.6 (24) 0.70 (28) 0.33 (08) 0.47 (16) 0.69 (17) 0.56 (07) 2.80 (29) 0.24(46)First dry period 200.20 ± 272.70 ± 299.25 ± 145.33 ± 123.13 ± 175.33 ± 145.00 ± 154.27 ±(days) 33.70(07) 35.80 (24) 56.17 (08) 34.91 (16) 33.27 (08) 83.52 (03) 12.00 (21) 19.23 (30)First service period 139.10 ± 141.20 ± 187.00 ± 204.21 ± 138.75 ± 150.66 ± 101.20 ± 140.13 ±(days) 9.30(11) 9.30 (39) 75.99 (04) 23.08 (16) 24.48 (08) 35.84 (03) 3.30 (131) 5.87 (39)Calving interval 552.0 ± 509.30 ± 431.35 ± 461.07 ± 425.38 ± 410.00 ± 443.00 ± 427.63 ±(days) 46.80 (07) 26.30 (24) 21.42 (19) 22.59 (41) 25.41 (08) 55.03 (21) 7.00 (52) 11.32 (30)Wet average (kg) 7.40 ± 6.00 ± 9.14 7.75 7.17 5.62 ± 7.25 ± 5.42 ±

0.60 0.60 0.16 0.10 0.09Dry average (kg) 4.70 ± 3.90 ± 6.86 4.82 3.47 3.61 ± 4.71 ± 4.00 ±

0.50 0.40 0.17 0.70 0.05

Figures in parentheses indicate number of observationsDR units at Bhiwani and Anjora, Durg were not included in the report as the data were not collected as per the requirement ofthe project*Peak yield

1647.22 ± 40.44 and 1894.40 ± 128.98 kg,respectively. The average first lactation lengthof Sahiwal cows at GLF-1, Hissar, Pantnagarand GADVASU, Ludhiana was 326.00 ±10.00, 296.09 ± 5.20 and 230 days,respectively. The wet and dry averages ofGLF-1, Hissar herd were 7.25 and 4.71 kg,respectively while the correspondingestimates for Pantnagar and GADVASU,Ludhiana were 5.42, 4.00 and 5.62 and 3.61kg, respectively.

Extension activities

The Gir and Kankrej units of the projectorganized farmers meet and conductedawareness camps for the conservation andgenetic improvement of the breeds. They alsomotivated the farmers to participate in thegenetic improvement programme to increasethe milk production of the indigenous cattlebreeds. As a consequence of these awarenessactivities more and more farmers are showingkeen interest to join the programme.



44

(II) INSTITUTIONAL PROGRAMMES

(A) MOLECULAR CHARACTERIZA-TION

Molecular characterization of autosomaland Y specific microsatellite markersrelated to milk production traits andfertility of Frieswal cattle

Development of an Artificial NeuralNetwork model for predicting post thawmotility of bull semen

The freezing significantly hampers themotility of sperm which may reduce theconception rate in dairy cattle. The predictionof post thaw motility before freezing will beuseful to take the decision on discarding orfreezing of the germplasm. The ArtificialNeural Network (ANN) methodology hasbeen found to be useful in prediction andclassification of problems related to animalscience and hence the present study wasundertaken to compare the efficiency of ANNin prediction of Post Thaw Motility (PTM)based on number of ejaculates, volume andconcentration of sperms. The combined effectof Y-specific microsatellite alleles on theactual and predicted PTM was also studied.The results revealed that the predictionaccuracy of PTM based on the semen qualityparameters was comparatively lower due tohigher variability in the data set. The ANNgave better prediction accuracy (34.88 percent) than the Multiple Regression Analysis(MRA) models (32.04 per cent). The RootMean Square Error (RMSE) was lower forANN (8.4353) than the MRA (8.6168). Thehaplotype or combined effect of microsatellitealleles on actual and predicted PTM wasfound to be highly significant (P<0.01). Basedon the results it was concluded that ANNmethodology can be used for prediction ofPTM in crossbred bulls but still requiresimprovement in efficiency of production.

Study of genetic polymorphism of heatshock protein genes among indigenousand crossbred cattle

Characterisation of ATPase beta familygenes during thermal stress incrossbred bulls

Na+/K+-ATPase is an integral membraneprotein composed of a large catalytic subunit(alpha), a smaller glycoprotein subunit (beta),and gamma subunit. The beta subunit isessential for ion recognition as well asmaintenance of the membrane integrity.Present study was aimed to analyze theexpression pattern of ATPase beta subunitgenes (ATPase B1, ATPase B2, and ATPaseB3) among the crossbred bulls under differentambient temperatures (20–44°C). Thepresent study was also aimed to look into therelationship of HSP70 with the ATPase betafamily genes. The results demonstrated thatamong beta family genes, transcriptabundance of ATPase B1 and ATPase B2was significantly (P<0.05) higher during thethermal stress. Pearson correlationcoefficient analysis revealed that theexpression of ATPase A1, ATPase B2, and

Fig. 11: Relative mRNA expression profile(mean±S.E.) of HSP70 and ATPase beta subunitgenes at different environmental temperatures

Rel

ativ

e E

xpre

ssio

n

Temperature (0C)

HSP70ATPaseB1ATPaseB2ATPaseB3

45



ATPase B3 is highly correlated (P<0.01) withHSP70, representing that the change inexpression pattern of these genes is positiveand synergistic (Fig-11). These may providea foundation for understanding themechanisms of ATPase beta family genesfor cellular thermotolerance in cattle.

Differential expression and SNPidentification of genes related toestablishment of pregnancy in Sahiwaland Frieswal cattle

Genomic DNA isolated from bloodsamples of 240 animals (81 Sahiwal and 159Frieswal) were used in the study. The 2,5-oligoadenylate synthetase 1 (OAS1) genewas selected for the study because of itsclear role during maternal recognition ofpregnancy. Five primer pairs were designedto clone four exons based on the GenBankreference sequence (bovine OAS1Ygene;Accession No. ENSBTAG00000039861)

using primer3 software. The PCRprogramme was made with the followingconditions—initial denaturation at 940C for 5min, followed by 35 cycles of 940C for 30 sec,specific annealing temperature for 30 sec,720C for 1 min and a final extension at 720Cfor 10 min. After the PCR, the requiredproduct sizes were amplified. The PCR-SSCP method was used to scan formutations within the amplified regions.Aliquots (10µL) of the PCR products weremixed with equal amount of denaturingsolution (95% formamide, 25 mM EDTA,0.025% xylene cyanole, and 0.025%bromophenol blue), heated at 950C for 5 minand immediately chilled on ice. The denaturedDNA was subjected to 10% polyacrylamidegel (acrylamide: bisacrylamide = 29:1)electrophoresis (PAGE) in 1× Tris/Borate/EDTA buffer with a constant voltage of 90 Vfor 14 h at a constant temperature. The gelwas stained with 0.1% silver nitrate.Variations were identified in SSCP patterns

Fig. 12. Illustration of PCR-SSCP carried out in the exonic regions of OAS1 gene. A, B and E: variation patternidentified in Exon1, 5 and first part of exon 6, respectively. C and D: monormorphic pattern detected in Exon 4

and second part of exon 6



46

of OAS1, 5, and 6A exonic regions, whereas monomorphic patterns were detected inOAS4 and OAS6B regions (Fig-12).

Screening of young Frieswal bulls forgenetic diseases

Frieswal bull calves were screenedagainst Bovine Leukocyte AdhesionDeficiency (BLAD), Deficiency of UridineMonophosphate Synthase (DUMPS), BovineCitrullinaemia and Factor XI Deficiency.Genomic DNA was extracted by phenol-chloroform method and the amplicons weregenerated by PCR. The PCR products weredigested with Taq1, AvaI and AvaII restrictionenzyme for BLAD, DUMPS and BovineCitrullinaemia, respectively. The digested

products and PCR product of Factor XIDeficiency were analyzed by agarose gelelectrophoresis stained with ethidiumbromide. Genotyping was done based on thebanding pattern in agarose gel (Fig-13).Among the 151 young Frieswal bulls carrierprevalence percentage of 4.6 was noticed forBLAD investigated while no carriers werenoticed for other genetic disorders studied.

Expression of fertility associated genesin sperm transcriptome of differentbreeds of cattle – A comparativeapproach

Different RNA isolation strategies such asTrizol method / Kit method with minormodifications in the existing protocols were

Fig. 13. Illustration of BLAD, DUMPS, Bovine Citrullinaemia and Factor XI Deficiency genotypes on agarose gels.Gel lanes were numbered from left to right and ladder used is 100 bp. (A). BLAD genotypes; Lane 2 showdigestion pattern for heterozygous animals with 3 bands of 357, 201 and 156 bp while other lanes (lanes 1,3,4,5,6 and 7) show normal homozygote pattern with 201 and 156 bp bands.(B). DUMPS genotypes; Lane 2,4,6and 8 were digested PCR products and Lane 1,3,5 and 7 were undigested products. All the digested productsshows normal homozygote pattern of 213 and 69 bp.(C). Bovine Citrullemia genotypes; Lane 2,4,6 and 8 wereundigested PCR products and Lane 3,5,7 and 9 were digested products. All the digested products showsnormal homozygote pattern of 109 and 89 bp. (D). FXID genotype; Lane 2 to 6 were PCR products showing the

size of 244bp normal of homozygote genotype

47



carried out to standardize the extraction ofRNA from frozen semen. The quality andquantity of extracted RNA were assessed byNanodrop UV Spectrophotometer. gDNAcontamination was tested using DAZLprimers which gave a band of 573 bp. RNAisolated by all the protocols showed gDNAcontamination but with different intensity (Fig-14). Optimization of DNase treatment for theelimination of gDNA contamination wascarried with different success rates.

Fig. 14. RNA isolation using different protocols. Fromleft to right; lane 1 Trizol method; lane 2 Modifiedprotocol; lane 3 Kit method; lane 4 Combination

approach; lane 5 gDNA as positive control; lane 6NTC. 100 bp marker

(B) SEMEN PRODUCTION ANDPRESERVATION

Semen production performance ofFrieswal bulls

Germplasm production

The average semen volume (ml), spermconcentration (million/ml), initial motility (%)and post thaw motility (%) were 4.73 ± 0.02,960.18 ± 5.49, 53.45 ± 0.24 and 41.71 ±0.30, respectively in 8,471 ejaculatescollected from Frieswal bulls during April2014-March 2015. About 6.02% ejaculateswere cloudy, 76.37% creamy & milky, 7.94%watery and 9.67% yellow. A total of 4,61,189semen doses were frozen in the year 2014,however, 3,37,485 semen doses fulfilling thefreezing standards were frozen during thefinancial year 2014-15. The semen quality inFrieswal bulls (Table-18) and number offrozen semen doses produced varied amongseasons. Highest number (1,09,839) ofsemen doses were produced in the hot & dryperiod, however, least (52,922) in the hot &humid period of the year. Initial and post thawmotility were significantly higher in winter thanin hot & humid period of the year. The mean

Quality Assessment and Processing of Semen for Cryopreservation



48

live spermatozoa percentages varied from69.01 ± 1.83 to 73.48 ± 3.97 among all bulls.Overall total morphological abnormalitieswere highest in the bulls aged above 60months. Highest abnormalities in mid pieceof spermatozoa were detected in aged bulls;however, tail abnormalities were least in thesebulls. Mean intact acrosome percentage washighest in young bulls than in aged bulls. Itranged from 69.42 ± 4.96 in the bulls of morethan 60 months of age to 76.62 ± 3.54 inyoung bulls. Mean HOST positivepercentage of spermatozoa varied from26.58 ± 2.41 to 39.12 ± 5.24 among all thebulls. Significant difference was observed formean HOST positive percentage of

spermatozoa between 36-60 month and 24-36 month age group bulls.

Effect of different housing systems onphysiological, behavioural and semenproduction performance of Frieswalbulls

An experiment was initiated to assess theperformance of Frieswal bulls managedunder two different housing systems duringwinter (December 2014-February 2015). Atotal of 31 adult Frieswal bulls weredistributed into 4 groups i.e. Traditionalhousing (T0); Concrete flooring (16 bullsdistributed over both sides of the shed) andImproved housing (T1); Concrete + kachcha

Table 18. Semen characteristics of Frieswal bulls

Period of the year Semen Sperm concentration Sperm initial Post thawvolume (ml) (million/ml) motility (%) motility (%)

Comfort (October – 4.62± 0.05 900.75± 10.91 53.48± 0.49 43.27± 0.66November 2014, March 2015)

Hot & dry (April to June 2014) 5.15±0.05 1137.01± 12.54 54.11± 0.46 40.32 ±0.59

Hot & humid (July-September 2014) 4.72± 0.04 949.35± 10.73 46.76± 0.47 38.79± 0.72

Winter (December 2014 to 4.45±0.04 865.12± 8.88 59.33± 0.43 43.46± 0.46February 2015)

Fig. 15. Percentage of time spent by bulls kept undertraditional housing systems (T0)

SC: Standing in covered area; SO: Standing in open area;StO: Sitting in open area; F: Feeding; B: Butting; Others(Sitting in covered area, rumination, drinking, urinationetc)

Fig. 16. Percentage of time spent by bulls kept underimproved housing systems (T1)

SP: Standing on pucca floor; SK: Standing on kachchafloor; StK: Sitting kachcha floor; F: Feeding; B: Butting;Others (Sitting on pucca floor, rumination, drinking,urination etc)

49



floorings (15 bulls distributed over both sidesof the shed). The observations on behaviourof bulls for three hours in day time wererecorded without disturbing them. The periodspent on different activities by the bulls in T0

and T1 has been presented in Figures 15 &16. The preliminary findings of the experimentrevealed that all the bulls preferred to spendmaximum time (69%) in the open area. Theperiod spent on standing in open area was37.2 and 19.4% in T0 and T1, respectively.However, animals of T0 and T1 spent 32.2 and44.4 % of total time on sitting activity duringthe observation period, respectively. Thesemen characteristics of fresh ejaculates ofthe Frieswal bulls maintained under the twohousing systems are presented in Table-19.

Quality assessment of Frieswal bullsemen

Frozen Frieswal semen was assessed forquality parameters as per the CMU guidelinesfor semen stations. Frozen semen doses of104 bulls were evaluated for post thaw motility,incubation at 37°C for 60 minutes, membraneintegrity by visualizing hypo osmotic swelling(HOS) test, acrosome integrity and spermconcentration (million/per straw).Simultaneously, twenty one new bulls thatwere inducted for semen collection and tenproblem bulls whose semen was frequentlyrejected due to poor initial motility were

assessed for viability and morphologicalabnormalities by eosin nigrosin stain. Theresults for various parameters are presentedin tables 20 and 21 and figures 17, 18 and19, respectively.

Table 19. Semen characteristics (Mean ± SE) of Frieswal bulls housed in different types of sheds

Group Semen volume Sperm Concentration Initial Motility(ml) (million/ml) (%)

I (Traditional: Concrete flooring & North open) 4.9 ± 0.40 1194.17 ± 113.91 57 ± 4.48

II (Traditional: Concrete flooring & South open) 4.3 ± 0.42 860.36 ± 90.02 58 ± 3.58

III (Improved: Concrete+kachcha flooring & East open) 4.3 ± 0.38 985.85 ±107.21 65 ± 3.66

IV (Improved: Concrete+kachcha flooring & West open) 3.8 ± 0.35 1059.00 ± 92.92 60 ± 3.96

Table 20. HOS reaction, acrosome intactnessand sperm concentration in frozensemen of Frieswal bulls

Parameters N Mean±SE

Post thaw motility (%) 104 54.57±0.64

Motility after incubation for 104 45.00±0.6930 min (%)

Motility after incubation for 104 37.23±0.7360 min (%)

HOS test (%) 104 56.18±1.14

Intact acrosome (%) 104 69.06±0.65

Concentration (million/straw) 104 26.75±0.61

Table 21. Viability and morphology of Frieswalbull semen (neat semen)

Parameters New bulls Problem bulls(%) (n=21) (n=10)

Live 72.58±2.48 64.62±7.59

Dead 27.42±2.48 35.38±7.59

Abnormal head 2.88±0.48 12.22±6.82

Abnormal mid piece 10.41±0.93 12.43±2.54

Abnormal tail 1.72±0.37 2.27±0.80

Overall abnormality 15.01±1.13 26.91±6.82



50

Fig. 17. Quality assessment in frozen semen of Frieswal bulls

Microbial load in frozen Frieswalsemen

A total of 69 frozen semen doses fromFrieswal bulls were subjected to bacterialload estimation by pour plate method. Themean colony count was 2594±312.38 cfu/ml.Sixty two samples (89.86%) were found tohave bacterial load within permissible limitwhile seven samples (10.14%) had bacterialcounts of more than 5000 cfu/ml (Fig-18).

Morphometric subpopulations inFrieswal bull semen

Preliminary analysis of semen samplesfrom 35 bulls showed that mean values forlength, width, perimeter and area of spermswere 8.93, 5.89, 23.91 µm & 36.22 µm2,respectively and the mean ellipticity, rugosity,elongation & regularity measures were 1.58,9.49, 0.20 and 1.14, respectively.

Fig. 18. Bacterial colony count in frozen Frieswal semen

Fig. 19. Fluorescent Staining: Pic. A, A1and B, B1: Spermatozoa with abnormalhead seen by CMA3 staining and underphase contrast (400x). YellowFluorescence depicts disintegratedDNA. Pic. C: Fluorescent stained live(green) and dead as (red) spermatozoa(400x) stained with SYBR-14.

51



(C) AUGMENTATION OF REPROD-UCTIVE EFFICIENCY

Enhancing reproductive efficiency inFrieswal heifers

A group of nine Frieswal heifers of MFMeerut which calved during November 2013to April 2014 were maintained and fedindividually as per Military Farm feedingschedule. The weighed quantity of dry andgreen fodder was offered daily and the refusalwas measured next day before offering freshfeed. The body weights were recorded atfortnightly interval. Their mean body weightat the time of first estrus exhibition aftercalving was 388.55±8.53 kg and at the timeof AI was 399.62 ± 9.77 kg. The average bodyweight of these animals at the start of lactationwas 407.22 kg. Out of nine cows, one diedduring experimentation and six wereconfirmed for various stages of pregnancy.The calvings are awaited.

Reproductive performance of field cattle

A total of 317 animals were examined inthe infertility camps organized in the nearbyvillages i.e. Arnavali, Pooth Khas (twice),Majra and Rasulpur. History of the animalsrevealed that 30% animals were anoestrusand 15.14% exhibited repeated breeding.Approximately 45% of animals were sufferingfrom other diseases. Anoestrus animals weregiven a combined preparation of mineralmixture and hormones orally (progesterone+ estrogen) and 63% of these animalsresponded to the treatment.

A total of 58 animals of PanchayatiGoshala, Hapur were also examined forvarious ailments and treated accordingly.

In the experiment conducted at Militaryfarm Meerut to assess the effect ofClomiphene + GnRH analogue on estrusinduction in 14 anestrus Frieswal heifers,85% exhibited oestrus compared to 70% incontrol group.

(D) IMPROVEMENT OF CATTLETHROUGH NUTRITIONAL MANIPU-LATION

Effect of micro-minerals on qualitativeand quantitative attributes of semen

Effect of zinc supplementation in the formof zinc sulphate was studied in Frieswal bulls.Based on available production data, a totalof 42 adult bulls, with 21 each of good andpoor semen category were selected fromBRU herd. Bulls of each category were furtherdivided into three groups and fed diets having40 ppm (diet 1), 60 ppm (diet 2) and 80 ppm(diet3) zinc per kg of diet on dry matter basis.The feeding trial of 6 month was started inSeptember 2014 and a digestibility trial wasconducted in March 2015.

The body weight of the experimental bullsis given in Table 22 and Figure 20. The bodyweight of all bulls increased in all the groups.Initial average body weights of good bulls fedon diets 1, 2 and 3 were 601±38.29,619±56.63 and 655±33.04 kg, respectivelyand the corresponding weights for poor bullswere 543±42.68, 543±40.94 and 528±24.99kg, respectively. Average body weights ofbulls after 6 months of feeding trail were692±30.45, 710±50.48 and 734±30.56 kg ingood bulls and 662±35.12, 672±29.69 and641±19.48 kg in poor bulls fed on diet 1, 2and 3, respectively.



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Digestibility of different nutrients in theexperimental bulls fed on different diets wasestimated. Average dry matter intake of goodbulls fed on diets 1, 2 and 3 was 9.86±0.43,10.59±0.41 and 9.52±0.53, respectively whilein poor bulls the estimates were 10.15±0.53,10.71±0.25 and 9.59±0.44, respectively. Dry

matter intake per 100 kg body weight waslowest in the bulls fed on diet 3 in both groupswhile it was highest in the bulls fed on diet 2in both the groups. Average dry matter intakeper 100 kg body weight of good bulls fed withdiets 1, 2 and 3 were 1.44±0.04, 1.48±0.05and 1.38±0.05 kg, respectively while in poorbulls the estimates were 1.53±0.09,1.59±0.08 and 1.52±0.05 kg, respectively.Similarly, average dry matter intake per kgmetabolic weight of good bulls fed with diets1, 2 and 3 was 73.65±2.11, 76.49±1.82 and70.65±2.80 gram, respectively while theestimates in poor bulls were 77.42±3.61,80.82±3.26 and 76.31±2.28 gram,respectively. Dry matter digestibility in theexperimental bulls was almost similar in allthe groups and the details are given in theTable 23.

Table. 22. Initial and final body weight (Kg) of experimental bulls

Category of bulls Type of Diet Initial Body Weight (Kg) Final Body Weight (Kg)(Aug-14) (Mar-15)

Good semen quality bulls Diet 1 601±38.29 692±30.45

Diet 2 619±56.63 710±50.48

Diet 3 655±33.04 734±30.56

Poor semen quality bulls Diet 1 543±42.68 662±35.12

Diet 2 543±40.94 672±29.69

Diet 3 528±24.99 641±19.48

Fig. 20. Initial and final body weight (Kg) ofexperimental bulls

Table. 23. Digestibility of nutrients in good and poor semen producer Frieswal bulls

Good semen quality bulls Poor semen quality bulls

Diet 1 Diet 2 Diet 3 Diet 1 Diet 2 Diet 3

Body weight 684.4 ± 719.80 ± 690.80 ± 670.40 ± 680.20 ± 629.80 ±(kg) 21.05 45.15 32.25 43.46 28.90 25.95

DMI (kg) 9.86 ± 0.43 10.59 ± 0.41 9.52 ± 0.53 10.15 ± 0.53 10.71 ± 0.25 9.59 ± 0.44

DMI/100 kg 1.44 ± 0.04 1.48 ± 0.05 1.38 ± 0.05 1.53 ± 0.09 1.59 ± 0.08 1.52 ± 0.05BW (kg)

DMI g/kg0.75 73.65 ± 2.11 76.49 ± 1.82 70.65 ± 2.80 77.42 ± 3.61 80.82 ± 3.26 76.31 ± 2.28

DMD (%) 59.43 ± 1.12 60.90 ± 1.03 59.60 ± 1.59 59.97 ± 1.37 61.04 ± 1.08 60.21 ± 2.02

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Proximate analysis of feed samples

Animal Nutrition Laboratory is providingfeed testing facility to the Military Dairy Farmsin terms of analysis of fodder, feeds and feedingredient. During this period a total 915 feedsamples received from 16 Military Farmswere analyzed for proximate principles. Thesamples analysed were mainly Wheat Branand Maize Whole followed by Mustard Cake,Deoiled Soybean Cake, Cotton Seed Cake,Groundnut Cake, Guar Meal, Wheat Straw,Barley and Deoiled Mustard Cake (Fig-21).

(III) EXTERNALLY FUNDED PROJECT

Molecular characterization and analysisof genetic polymorphism in Integrinalpha beta 6 receptor gene associatedwith Foot-and-Mouth Disease Virus(FMDV) cell tropism in cattle (DST)

Development of a Tetra ARMS PCRbased genotyping of FMDV host receptorat 5’ UTR region of ITGB6 gene in cattle

Integrin Beta-6 (ITGB6) is known to be one ofthe major receptor components involved in hosttropism of Foot-and Mouth Disease (FMD) virusin cattle. A competitive PCR technique calledARMS PCR was adapted to identify a single-nucleotide polymorphism (SNP), G29A, db SNPId: rs109075046, at the 5’untranslated region (5’UTR) of the bovine ITGB6 gene. Genotype profilingrevealed, three genetic variants within the targetedSNP among Frieswal crossbred cattle (Fig-23).AA genotypes were widely distributed amongindigenous breeds, while frequency ofhetrozygotes were higher in Frieswal (Table 24).Further, chi square analysis revealed a strongassociation between genotypes and incidence ofFMD (Table 25).

Fig. 22. Number of feed samples received fromdifferent Military Farms

Fig. 21. Feed ingredients received from differentMilitary Farms for proximate analysis

Maximum samples were received fromMilitary Farm Ambala followed by Meerut,Secunderabad, Namkum, Jhansi, BRUMeerut, Lucknow, Agra, Mhow, Bareilly,Ranikhet, Binnaguri, Jabalpur, Deolali,Ahmednagar and Jallandhar (Fig-22).

Fig. 23. Agarose gel electrophoresis of the tetra-primer PCR assay products. Lane 1: GG genotype (659and 257 bp), Lane 2,3,8,9,10,11,14 & 15: AA genotype(659 and 445 bp), Lane 4, 5,6,7,12& 13 : GA genotype

(659,445 and 257 bp)



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Genetic charcterisation of ITGB6receptor exonic region among differentZebu breeds

For analyzing the scenario of targetedSNP among different Zebu cattle, we includedfour indigenous breeds viz. Sahiwal (n= 51),Kankraj (n= 48) , Ongole (n= 38) and Gir

Table. 24. Genotype and Allele frequency of the SNP G29A mutation at the 5’UTR region of theITGB6 gene among different cattle breeds

Breeds Genotype frequency Total Allele frequency Chi-Square

AA GA GG A G

Frieswal 65 (0.320) 125 (0.611) 14 (0.069) 204 0.626 0.374 19.25**

Sahiwal 34 (0.667) 17 (0.33) 0 51 0.833 0.167 2.04ns

Kankrej 48 0 0 48 1.00 0.00 -Ongole 38 0 0 38 1.00 0.00 -Total 185 142 14 341

** P < 0.001; ns non-significant.

Table. 25. Genotype and allele frequency of the SNP G29A mutation at the 5’UTR of ITGB6 geneamong FMDV affected (case) and non-affected (control) animals

Class Genotype frequency Total Chi-Square

AA GA GG

Case 5 42 8 55 18.24**

Control 15 18 0 33Total 20 60 8 88

** P < 0.001

Fig. 24. Dendogram analysis for the different genotypes sequenced A. GG (KJ847280); B.GA (KJ847281) andC. AA (KJ847282) genotypes

(n=11) of cattle from different agriculturalzones.

The present study was aimed to developa single tube tetraplex PCR based genotypingassay for SNP (rs13650029) at exonic regionof bovine ITGB6 receptor gene. Genotypingrevealed that the genotype TT was widely

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distributed among the targeted Zebu cattlebreeds. The study revealed that the frequencyof T allele was higher in indigenouspopulations compared to the C allele. The Tallele was mostly observed in the homozygotecondition than in heterozygotic individuals.This trend was mostly seen in all the breedsexcept Sahiwal where the heterozygoticfrequency was comparatively higher, eventhough the difference was not statisticallysignificant. The test for HW equilibriumamong different populations showed that allthe breeds were under equilibrium withrespect to the ITGB6 receptor gene indicatingthe absence of aggressive selection i.e.selection primarily based on this gene(P<0.05). The frequency of animals havingCC genotype was lowest in all the fourbreeds. In order to study the variation in thegenotype frequencies among differentindigenous breeds of cattle, Chi-square testwas performed. The result showed that thegenotype frequencies were not statisticallydifferent (P = 0.2455) among the breedsindicating that all the populations were havingsimilar genetic constitution with regard to theITGB6 receptor gene. The SNP(rs136500299) T/C located at the position2145 of the reference ITGB6 mRNAproduces a missense change Phe/ Ser.Serine is a polar amino acid with its smallersize, whereas phenylalanine is an aromaticamino acid with complex structure. Thus, itmay be presumed that changes of amino acidmay alter the conformational changes of theITGB6 receptor coding polypeptide, whichhowever, needs to be confirmed through morestudies. The results of the present studysuggested that T allele is widely distributedamong the indigenous breeds of cattle whichmay be associated with the resistance toFMD virus, as the susceptibility to FMD virusis lower than the tauras breeds. However,

studies with larger sample sets and widerange of cattle breeds are still needed toconfirm the exact genetic distribution patternof the SNP.

(IV) EXTENSION ACTIVITIES

1. Participation in Kisan mela

During the report period ICAR-CIRCparticipated in 7 Kisan Melas organized bydifferent Institutions. Major activities andtransferrable technologies developed by theInstitute were depicted at ICAR-CIRC Stall inthe Melas. Dr. Sanjeev Kumar Verma, SeniorScientist (Animal Nutrition) & CoordinatorICAR-CIRC stall and the participatingscientists explained the farmers aboutbreeding, feeding and health managementof livestock. During the exhibition, largenumber of farmers visited ICAR-CIRC stalland were distributed with leaflets on activitiesundertaken by the Institute on differentaspects of Cattle Production andManagement. The exhibits of CIRC stall weregreatly appreciated by the visitors andfarmers. The details of CIRC participation indifferent melas are given in the Table 26.

2. Organisation of farmer awarenesscamp

A one day Animal Husbandry AwarenessCamp was organized at village Bhagwanpur,Block Mawana, District Meerut on 20th June2014 in collaboration with Jan KalyanSanstha (a Non Profitable VoluntaryOrganisation), Meerut and NABARD, Meerut.In this awareness camp, Dr. Pramod Singh,Principal Scientist (Animal Nutrition); Dr.Sanjeev Kumar Verma, Senior Scientist(Animal Nutrition) and Dr. Ajay Veer SinghSirohi, Senior Scientist (LivestockProduction & Management) participated and



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Table. 26. Participation in Kisan Mela & Agri Fairs

S. Details of the Venue Date No.of Scientist who participatedNo. Programme farmers in Kisan Mela

who visitedCIRC stall

1 Kisan Mela Evam Krishi SVPUAT, 16-18 780 Dr. SK Verma (Stall Coordinator)Udhyog Pradarshani Meerut Oct 2014 Dr. Pramod Singh

Dr. S SahaDr. AVS SirohiDr. Nemi ChandDr. N SrivastavaDr. Saroj RaiDr. Rajib DebDr. RR AlythodiDr. Rani AlexDr. Megha PandeDr. YK Soni

2 IIWM, Bhubaneswar & Village Rasulpur, 13 Dec 136 Dr. SK Verma (Stall Coordinator)IIFSR, Meerut Distt. 2014 Dr. AK Mathur

Muzaffarnagar Dr. Rajendra PrasadDr. Sushil KumarDr. Megha Pande

3 IIWM, Bhubaneswar & Village Barwala, 14 Dec 113 Dr. SK Verma (Stall Coordinator)IIFSR, Meerut Distt. 2014 Dr. Sushil Kumar

Muzaffarnagar Dr. Nemi Chand

4 Kisan Vigyan Samagam IIFSR, Meerut 23 Jan 53 Dr. SK Verma (Stall Coordinator)2015 Dr. S Saha

Dr. YK Soni

5 12th Agricultural Science NDRI, Karnal 3-6 Feb 130 Dr. SK Verma (Stall Coordinator)Congress and ASC 2015 Dr. S TyagiIndia Expo Dr. Umesh Singh

Dr. YK Soni

6 Regional Agricultural CPRS, Patna 19-21 681 Dr. SK Verma (Stall Coordinator)Fair Feb 2015 Dr. AVS Sirohi

7 Indian Agri Show Pragati Maidan, 26-28 366 Dr. SK Verma (Stall Coordinator)New Delhi Feb 2015 Dr. AK Mathur

delivered lectures to the farmers regardingscientific feeding of newly born calf andbalanced feeding of different categories ofanimals. Dr. Sirohi highlighted the importanceof proper housing management for profitablelivestock production. In the whole villageconsisting of 294 farm families, a total 1001livestock population were being reared. Outof which 356 animals were in milk.

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3. Organization of Infertility camps

The following infertility camps wereorganized by the institute during the year2014-15 in the rural areas around Meerut.

In total, 8 infertility camps and 5 follow-upvisits were organized where 389 animalswere successfully treated and followed fordifferent reproductive, nutritional, parasiticand other health related ailments. Duringthese camps, farmers were also made awareof the importance of balanced feeding,proper record keeping, timely insemination

and pregnancy diagnosis of their animals toharvest maximum production potential ofdairy animals.

Table. 27. Infertility camps organised by CIRC

S. Date and Place Scientists Participated No. and type of cases/ Treatment/Advice givenNo. others if any

1. 6 May 2014, Arnavali Dr. AK Mathur 31 (Anestrus, Repeat breeding, Mineral supplements,Dr. R Prasad Parasitic infestations) Dewormers, Ectoparasiticides,Dr. S Tyagi Hormonal treatmentDr. DK MandalDr. Megha PandeDr. YK Soni

2. 22 Aug. 2014, Dr. AK Mathur 105 (Anestrus, Repeat breeding, Mineral supplements,Pooth Khas Dr. S Tyagi Nutritional imbalance, Parasitic Dewormers, Ectoparasiticides,

Dr. SK Verma infestations) Hormonal treatmentDr. Megha PandeDr. YK Soni

3. 13 Nov. 2014, Majra Dr. AK Mathur 40 (Anestrus, Repeat breeding, Mineral supplements,Dr. SK Verma Parasitic infestations) Dewormers, Ectoparasiticides,Dr. Naimi Chand Hormonal treatmentDr. Megha PandeDr. YK Soni

4. 17 Nov. 2014, Dr. AK Mathur - Proper feeding andPanchayati Gaushala, Dr. SK Verma management including recordHapur keeping of the animals was

advised

5. 28 Nov. 2014, Dr. AK Mathur 22 (Anestrus, Repeat breeding, Hormonal treatment, anti-Panchayati Gaushala, Dr. Rajendra Prasad Prolapse, Mastitis) microbialsHapur Dr. Naimi Chand

Dr. Megha PandeDr. YK Soni

6. 15 Dec. 2014, Dr. AK Mathur 36 (Anestrus, Repeat breeding, Hormonal treatment, anti-Panchayati Gaushala, Dr. Rajendra Prasad AI, Pregnancy Diagnosis) microbials, AI of animalsHapur Dr. Naimi Chand exhibiting estrus.




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S. Date and Place Scientists Participated No. and type of cases/ Treatment/Advice givenNo. others if any

7. 06 Jan. 2015, Dr. S Saha Follow-up visit Hormonal TreatmentPanchayati Gaushala, Dr. YK SoniHapur

8. 11 Feb. 2015, Dr. AK Mathur Follow-up of the infertile Response to treatment wasPanchayati Gaushala, Dr. SK Verma animals, Nutritional deficiency recorded, Urea treatment of theHapur Dr. Naimi Chand was observed Paddy straw was demonstrated


9. 21 Feb. 2015, Dr. N Srivastava Follow-up of infertility cases and Prolapse reposed successfully,Panchayati Gaushala, Dr. Megha Pande Cervico-vaginal prolapse anti-microbial and anti-Hapur Dr. YK Soni histaminic therapy

10. 25 Feb. 2015, Dr. AK Mathur 57 (Anestrus, Repeat breeding, Mineral supplements,Pooth Khas Dr. SK Verma Parasitic infestation, AI, Dewormers, Ectoparasiticides,

Dr. S Saha Pregnancy Diagnosis) Hormonal treatmentDr. Naimi ChandDr. Megha PandeDr. YK Soni

11. 12 March 2015 Dr. SK Verma Follow-up of the infertility cases Response to treatment wasPanchayati Gaushala, Dr. YK Soni recorded, Urea treated paddyHapur straw was opened and adviced

to feed the animals

12. 30 March 2015, Dr. AK Mathur 84 (Anestrus, Repeat breeding, Mineral supplements,Rasulpur Jatan Dr. SK Verma Parasitic infestation, AI, Dewormers, Ectoparasiticides,

Dr. Naimi Chand Pregnancy Diagnosis) Hormonal treatment, AI,Dr. YK Soni Pregnancy dignosis

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Glimpses of infertility camps organized by ICAR-CIRC

(V) TECHNOLOGY/CONCEPT

Technology Developed

● The allelic variations of the loci targetedfor association studies with certain bull-semen quality parameters revealed thatpolymorphic loci of INRA 126 bulls with182 and 184 alleles had significantlyhigher semen volume as compared to186 alleles, however, 186 alleles showedsignificantly higher concentration per mlof semen compared to 182 and 184. Thestudy also revealed that the Y-specificMicrosatellite alleles may be useful asbiomarkers for differentiating bulls withgood and poor quality semen.

Inventors: Rajib Deb, Sushil Kumar, UmeshSingh, Shrikant Tyagi, DK Mandal and AKMathur

ICAR News- A Science and TechnologyNewsletter, 2014. Vol-20 No. 4

● A single tube tetra ARMS PCR-basedassay for rapid genotyping of the 5’ UTRregion of bovine Integrin beta 6 receptorgene, associated with Foot-and-mouthdisease virus (FMDV) host tropism, hasbeen developed. Four sets of primers wereemployed: Outer Forward, Outer Reverse,Inner Forward and Inner Reverse. Theywere designed to amplify fragments ofdifferent sizes for each allele band in orderto easily resolve variants using agarose gelelectrophoresis. To increase specificity ofthe reaction, a mismatch was introduced atthe 3’ end of each of the two allele-specificprimers. Identified genotypes weresubjected for their association with infectedand non-infected animals, which wereshown to be associated significantly withthe FMDV susceptibility.

Inventors: Rani Singh, Rajib Deb, UmeshSingh, Rani Alex, Sushil Kumar, SoumenduChakraborti, Sheetal Sharma, GyanendraSengar, Rupali Singh and Birham Prakash

ICAR News- A Science and TechnologyNewsletter, 2015. Vol-21 No. 1



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RESEARCH PROJECTS

The followings research projects were in operation during the year 2014

AICRP PROJECTS

Sl.No. Project Title Research Workers

1. Studies on genetic aspects of Holstein- Dr. Sushil Kumar (PI)Sahiwal crossbreds - “Frieswal Project” Dr. Rajendra Prasad

Dr. S TyagiDr. Ajaveer Singh SirohiDr. Neeraj ShrivastavaDr. Rajib Deb (from Sept. 2014)Dr. Rani AlexCo-ordinating unit I/C:Col. Jagjit Baswana

2. Genetic studies on performance of important Dr. Umesh Singh (PI)indigenous breeds of cattle and their Dr. TV Rajaimprovement through selection – “Indigenous Dr. Rafeeque R AlyethodiBreeds Project” In-charge Associated units:

Sahiwal : Dr. AK GuptaGir : Dr. PU GajbhiyeKankrej : Dr. JB Patel

3. Field recording of performance data for undertaking Dr. AK Das (PI)large scale progeny testing – “Field Progeny Dr. Ravinder KumarTesting of Frieswal Bulls” Dr. SK Rathee

In-charge associated units:GADVASU : Dr. PP DubeyKAU : Dr. K Anil KumarBAIF : Dr. SB GokhaleGBPUA&T : Dr. CB Singh

INSTITUTIONAL PROJECTS


1. Differential expression and SNP identification of genes related Dr. Rani Alexto establishment of pregnancy in Frieswal and Sahiwal cattle Dr. Umesh Singh

Dr. Sushil Kumar

2. Early selection of Frieswal sires using test day records Dr. TV RajaDr. R KumarDr. SK Rathee

3. Expression of fertility associated genes in sperm transcriptome Dr. R Alyethodiof different breeds of cattle –A comparative approach Dr. S Tyagi

Dr. AK DasDr. Rani AlexDr. Saroj Rai

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4. Genetic studies on disposal pattern in Frieswal cattle Dr. SK RatheeDr. AK DasDr. TV Raja

5. Baseline survey on cattle to multiply superior germplasm in Dr. R Kumarfield conditions for enhanced milk productivity Dr. Anil Kumar IIFSR,

Meerut

6. Assessment of factors affecting productive herd life and lifetime Dr. DK Mandalproduction of Frieswal bulls Dr. S Tyagi

Dr. M Kumar

7. Functional analysis of sperm morphometric subpopulations Dr. Mahesh Kumarin Frieswal bulls Dr. DK Mandal

Dr. Saroj RaiDr. S Saha

8. Effect of different housing systems on physiological, behavioural Dr. AS Sirohiand semen production performance of Frieswal bulls Dr. DK Mandal

9. Improvement of reproductive efficiency of cattle through different Dr. S Sahaphysiological and reproductive techniques Dr. AK Mathur

Dr. YK Soni

10. Augmenting the reproductive efficiency of cattle in organized Dr. YK Sonifarms and rural areas around Meerut through various reproductive Dr. AK Mathurand nutritional interventions Dr. S Saha

Dr. Megha PandeDr. Naimi Chand

11. Investigation on Fertility-Associated Antigen in sperm Dr. Megha Pandemembranes and seminal plasma vis-à-vis semen quality Dr. N Srivastavaparametres and freezability of Frieswal bulls Dr. M Kumar

Dr. YK Soni

12. Optimization of dietary energy in periparturient Frieswal cows Dr. Rajendra PrasadDr. Pramod SinghDr. SK Verma

13. Micronutrient status in the feeds and effect of dietary Dr. Pramod Singhsupplementation on growth and semen quality of Frieswal Dr. R Prasadbull calves Dr. SK Verma

14. Screening for genetic diseases in Frieswal and Indigenous bulls Dr. RR Alyethodi

15. Enhancing reproductive efficiency in Frieswal heifers by various Dr. AK Mathurreproductive techniques, nutrition and management interventions Dr. Rajendra Prasad

16. Effect of different levels of micro-minerals on qualitative and Dr. Sanjeev Kumar Vermaquantitative attributes of semen in Frieswal bulls. Dr. Rajendra Prasad

Dr. Pramod Singh

17. Molecular characterization of autosomal and Y specific micro Dr. Rajib Debsatellite markers related to milk production traits and fertility Dr. Umesh Singhstatus of Frieswal cattle Dr. Sushil Kumar

Dr. Rafeeque R Alyethodi



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18. Quality assessment on Frieswal bull semen Dr. Saroj RaiDr. Ajay Vir Singh SirohiDr. Naimi Chand

19. INTER-INSTITUTIONAL PROJECT: Study of genetic Polymorphism Dr. Basavraj Sajjanarof heat shock protein genes among indigenous and crossbred cattle Dr. Rajib Deb

EXTERNALLY FUNDED PROJECT (DST)


20. Molecular Characterization and Analysis of genetic polymorphism Dr. Rani Singhin Integrin alpha beta 6 receptor gene associated with Foot-and- Dr. Rajib Debmouth disease virus (FMDV) cell tropism in cattle (DST) Dr. Umesh Singh

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PUBLICATIONS

RESEARCH PAPERS

1. Chand N, Randhawa CS, Singh N D andBansal BK. 2014. Osteosarcoma in acrossbred cow. Indian VeterinaryJournal 91(5):65-66.

2. Deb R, Sajjanar B, Singh U, Alex R, RajaTV, Alyethodi, RR, Kumar S, Sengar G,Sharma S, Singh R and Prakash B.(2015). Understanding the mechanismsof ATPase beta family genes for cellularthermo tolerance in crossbred bulls.International Journal ofBiometeorology- DOI 10.1007/s00484-015-0986-x

3. Deb R, Singh U, Kumar S, Singh R,Sengar G and Sharma A. 2014. Profilingof Bovine Breast Cancer 1, Early Onset(BRCA1) Gene among Frieswal (HF 3Sahiwal) Cows and Their Associationwith Mastitis. National AcademyScience Letters 37 (6):579-583

4. Kumar S, Singh U, Ganguly I, Deb R,Singh R, Sandeep M, Sengar G, MandalDK, Kumar M and Sharma A. 2014.Protamine 3 expressions in crossbredbull spermatozoa may not be aprognostic marker for differentiatinggood and poor quality semen. AfricanJournal of Biotechnology 13 (20): 1999-2003.

5. Kumar S, Alex R, Singh U, Kumar A andDas AK 2015. Comparativeperformance evaluation of Frieswal bullsin organized farms and farmers’ herds.Indian Journal of Animal Sciences 85(3): 316–319.

6. Kumar S, Deb R, Singh U, Ganguly I,Mandal DK, Tyagi S, Kumar M, SengarG, Sharma S, Singh R and Singh R.2015. Bovine Circadian LocomotorOutput Cycles Kaput (CLOCK) andClusterin (CLU) mRNA quantitation inejaculated crossbred bull spermatozoa.Reproduction in Domestic AnimalsDOI: 10.1111/rda.12522

7. Kumar S, Deb R, Singh U, Ganguly I,Mandal DK., Singh R, Sharma S, SinghG, Singh R, Kumar M and Sharma A2014. SNPs at exonic region ofaquaporin- 7 (AQP-7) gene may effectsemen quality parameters amongcrossbred bulls, Journal of Genetics 93:108-112.

8. Manoj M, Gandhi RS and Raja TV. 2014.Effect of sex and nongenetic factors onbirth weight of Sahiwal calves. IndianJournal of Animal Sciences 84: 911-912

9. Monalisa D, Gandhi RS, Raja TV, AvtarS and Sachdeva GK. 2014. Geneticstudies on monthly test-day milk recordsin Sahiwal cattle. Indian Journal ofAnimal Sciences 84: 706-708.

10. Prakash B and Deepika. 2014.Assessment of genetic diversity andadmixture in two newly recognized cattlebreeds of Eastern India. Indian Journalof Animal Sciences 84: 752-756.

11. Pundir RK, Singh PK, Neelkant, SharmaD, Kumar S, Tiwari R, Singh CV andPrakash B. 2014. Characterization andevaluation of Hill cattle of Garhwal region



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of Uttarakhand, India. Indian Journal ofAnimal Research DOI : 10.5958/0976–0555.2014.00450.6.

12. Raja TV and Gandhi RS. 2014.Evaluation of Sahiwal sires using partlactation records for increasing the milkproduction. Indian Journal of AnimalSciences 84: 1321–1324.

13. Raja TV and Gandhi RS. 2014. Relativeefficiency of part lactation records inselection of Sahiwal sires. IndianJournal of Animal Sciences 84: 876–879.

14. Ranjan S, Bhushan B, Panigrahi M,Kumar A, Deb R, and Sharma D. 2015.Association and expression analysis ofsingle nucleotide polymorphisms ofpartial tumor necrosis factor alpha genewith mastitis in crossbred cattle. AnimalBiotechnology 26(2): 98-104.

15. Sahoo AP, Tiwari AK, Kumar GR,Chaturvedi U, Singh LV, Saxena S, PaliaSK, Jadon NS, Singh R, Singh KP,Prakash B, Maiti SK and Das AK. 2014.Establishment and characterization of abovine rectal myxoma cell line. Tissueand Cell 47: 49-54.

16. Singh G, Randhawa S S, Nayar S, ChandN and Randhawa C S. 2014. Evaluationof oxidative stress during periparturientperiod in crossbred cows. Intas Polivet15:188-191.

17. Singh R, Deb R, Singh U, Raja TV, AlexR, Kumar S, Chakraborti S, Sengar Gand Sharma S. 2014. Single tubetetraplex PCR based screening of aSNPs at exon 14 region of bovine ITGB6

among different Zebu breeds. MetaGene 10 (3): 26-30.

18. Singh R, Deb R, Singh U, Alex R, KumarS, Soumendu C, Sharma S, Sengar G,Singh R. 2014. Development of a tetra-primer ARMS PCR-based assay fordetection of a novel single-nucleotidepolymorphism in the 5’untranslatedregion of the bovine ITGB6 receptor geneassociated with foot-and-mouth diseasesusceptibility in cattle. Archives ofVirology 159 (12): 3385-9

19. Singh R, Deb R, Singh U, Raja TV, AlexR, Kumar S, Alyethodi RR, Sahu J, DuttaR, Sengar G, Sharma S. 2015. StructuralInsight of Bos Indicus ITGB6 ReceptorGene for FMDV Host Tropism. Advancesin Animal and Veterinary Sciences 2:549-556.

20. Singh ST, Dua K, Chand N, RandhawaCS and Gupta MP. 2014. Seasonalvariations in mineral status of dairyanimals in fluoride endemic semi-aridSouth-West Punjab. Indian Journal ofVeterinary Medicine 35:60-62.

21. Singh S, Das AK, Chakraborty D, TaggarRK, Kumar N, Gupta P and Mahajan V.2014. Studies on genetic and non-genetic factors affecting performancetraits of Frieswal cows. Indian Journalof Animal Research 48(6): 537-540

22. Singh U, Deb R, Kumar S, Singh R,Sengar G and Sharma A. 2014.Association of prolactin and beta-lactoglobulin genes with milk productiontraits and somatic cell count amongIndian Frieswal (HFxSahiwal) cows.Biomarkers and Genomic MedicineDOI: 10.1016/j.bgm.2014.07.001.

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23. Singh U, Kumar S, Ganguly I, Gaur GK,Jagadeesan K, Kumar S, Mann S andSingh R. 2014. Identification of geneticpolymorphism in two exonic codingregion of leptin gene among indigenousand crossbred cattle. Indian Journal ofAnimal Research 48 (5): 403-407.

24. Soni YK, Mehrotra S, Singh G, Das GK,Kumar A, Awase M and Gahlot M. 2014.Oxidative stress and progesteroneprofile in repeat breeding cows.Veterinary Practitioner 15(2): 213-214.

25. Taggar RK, Das AK, Kumar D andMahajan V. 2014. Genetic analysis ofperformance traits of Jersey cows in sub-temperate environment of HimanchalPradesh, India. Indian Journal of AnimalResearch 48(5): 413-417.

26. Taggar RK, Das AK, Kumar D andMahajan V. 2014. Genetic studies onperformance traits of Jersey crossbredcows in sub-temperate region. IndianJournal of Animal Science 84(7): 771-774.

REVIEW PAPERS

1. Das AK, Gupta P and Chakraborty D.2015. Physical Methods of GeneTransfer: Kinetics of Gene Delivery intoCells. Agricultural Reviews 36(1): 61-66.

2. Singh U, Deb R, Alyethodi RR., Alex R,Kumar S, Chakraborty S, Dhama K andSharma A. 2014. Molecular markers andtheir applications in Cattle GeneticResearch: A review. Biomarkers andGenomic Medicine 6: 49-58.

BOOKS/BOOK CHAPTER

1. Alex R, Alyethodi RR, Singh U, KumarS, Deb R. 2015. Marker BasedTechnologies: A paradigm shift inselection methodologies in livestock.Singh U, Kumar S, Mukhopadhyay CS,Deb R, Alyethodi RR, Alex R, Dhama K,Ganguly I (Eds.). 2015. In: Bioinformaticapproaches for livestock genomeanalysis, Satish Serial Publishing, NewDelhi, India, ISBN: 978 9384 053017.pp:295-312.

2. Alyethodi RR, Alex R, Singh U, KumarS, Deb R. 2015. Transcriptome Analysis:Methods and Applications. In Singh U,Kumar S, Mukhopadhyay C S, Deb R,Alyethodi, R R, Alex R, Dhama K,Ganguly I (Eds.). 2015. In: Bioinformaticapproaches for livestock genomeanalysis, Satish Serial Publishing, NewDelhi, India, ISBN: 978 9384 053017. pp:201-246.

3. Das AK, Raja TV, Deb R, Singh U,Kumar S. 2015. Question Bank onAnimal Genetics & Breeding. Pages 1-195. Satish Serial Publishing House.Delhi. ISBN: 978-93-84053-13-0.

4. Prakash B. 2014. Farm animal geneticdiseases database: Significance inanimal improvement. In: “Computationaltools for Animal genome resource dataAnalysis” pp 129-145. NBAGR Karnal.ISBN No.978-93-83537-10-5.

5. Singh U, Kumar S, Mukhopadhyay CS,Deb R, Alyethodi RR, Alex R, Dhama K,Ganguly I. 2014. BioinformaticsApproaches for Livestock GenomeAnalysis. Satish Serial Publishing



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House, New Delhi, pp: 1-312 ISBN 978-93-84053-01-7.

ACCESSION NUMBERS

1. KJ 847280 Singh R., Deb, R.,Chakraborti, S., Kumar, S. and Singh, U.[Bos indicus x Bos taurus] 5’UTR regionof ITGB6 receptor gene-GG genotype.

2. KJ847281 Singh, R., Deb, R.,Chakraborti, S., Kumar, S. and Singh, U.[Bos indicus x Bos taurus] 5’UTR regionof ITGB6 receptor gene-GA genotype

3. KJ847282 Singh, R., Deb, R.,Chakraborti, S., Kumar, S. and Singh, U.[Bos indicus x Bos taurus] 5’UTR regionof ITGB6 receptor gene-AA genotype

MONOGRAPHS/TRAINING MANUALS/PAMPHLETS

1. Mandal DK, Kumar Mahesh, Tyagi S andPrakash B. 2015. Characterization ofFrieswal bulls: Body morphometric traits,Semen quality and Cytogenetic profile.Published by ICAR-Central Institute forResearch on Cattle, Meerut Cantt, pp 1-40.

2. Pande M, Verma SK and Singh P. 2014.

xkS&i’kqvksa ds mipk;h jksxA Publishedby ICAR-Central Institute for Research onCattle, Meerut Cantt. pp-1-4.

3. Singh U, Kumar S, Das AK, Kumar R,Raja TV, Rathee SK, Deb R, AlyethodiRR, and Rani A. 2014. Training manualof DBT sponsored training programmeon Molecular Tools and BioinformaticsApproaches for Livestock GenomeAnalysis. Published by Central Institutefor Research on Cattle, Meerut Cantt.

4. Soni YK and Mathur AK. 2014. xkS&i’kqvksaesa ckW>iuA Published by ICAR-CentralInstitute for Research on Cattle, MeerutCantt. pp-1-4.

5. Tyagi S, Kumar M, Mandal DK, SirohiAS, Srivastava N, Rai S. 2014. TrainingManual of Model Training Course onProduction, Processing and Certificationof Quality Bovine Semen. Published byCentral Institute for Research on Cattle,Meerut Cantt.

6. Verma SK, Singh P, Pande M and Soni

YK. 2014. oSKkfud fof/k ls xksoa'k ikyuAPublished by ICAR-Central Institute forResearch on Cattle, Meerut Cantt. pp-28.

7. Verma SK, Singh P, Pande M and Chand

N. 2015. O;ogkfjd xkso a'k ikyuAPublished by ICAR-Central Institute forResearch on Cattle, Meerut Cantt. pp-28.

8. Verma SK, Singh P, Pande M and Sirohi

AVS. 2015. xksoa'k ikyu ds oSKkfud rF;APublished by ICAR-Central Institute forResearch on Cattle, Meerut Cantt. pp-28.

INVITED LECUTURES/LEAD PAPER

1. Mathur AK. 2014. Pervasiveness andcontrol of occupational zoonoses inanimal gynaecologists. Nationalcongress on veterinary public health andXI conference of association of publichealth veterinarians: present status andfuture road map. NASC Complex, NewDelhi. 24-25 November 2014, pp. 192-196.

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2. Mathur AK and Mandal DK. 2014.Appraisal of challenges & opportunitiesfor Marginal and small holders DairyFarming Systems involving indigenouscattle breeds. Silver jubilee conventionof ISAPM and National seminar onRevisiting management policies andpractices for indigenous livestock &poultry breeds as eco-friendly economicproducers. Navsari AgricultureUniversity, Navsari 9-11 October 2014,pp 115-123.

3. Prakash B. 2014. Genetic diseases offarm animals: a serious health hazard.National Symposium on Harmonizingphenomics and genomics forsustainable management of livestock forupliftment of rural masses. NBAGRKarnal, 6-7 February 2014, pp 38-50.

4. Prakash B, Singh U, Kumar S, Raja TVand Alex R. 2015. Genetic Improvementof Cattle Genetic Resources of India:Issues and Challenges. InternationalSymposium on SustainableManagement of Animal GeneticResources for Livelihood Security inDeveloping Countries & XII AnnualConvention of Society for Conservationof Domestic Animal Biodiversity. MadrasVeterinary College, Chennai, 13-14February 2015, pp-23-30.

LECTURES DELIVERED IN TRAININGPROGRAMES

1. Alex R, Alytheodi RR, Deb R andSharma S. 2014. Molecular tools foridentifying genetic variation. In TrainingManual: Molecular tools andbioinformatics approaches for livestockgenome analysis, held at Central

Institute for Research on Cattle, Meerutfrom 10-30, September, 2014, pp. 171-177.

2. Alex R, Singh U, Kumar S and Raja TV.2014. Data analysis using SAS – Basicconcepts. In Training Manual: Moleculartools and bioinformatics approaches forlivestock genome analysis, held atCentral Institute for Research on Cattle,Meerut from 10-30, September, 2014,pp. 76-81.

3. Alytheodi RR, Deb R, Sharma S andSengar G. 2014. Isolation and culturingof bovine pheripheral bloodmononuclear cells. In Training Manual:Molecular tools and bioinformaticsapproaches for livestock genomeanalysis, held at Central Institute forResearch on Cattle, Meerut from 10-30,September, 2014, pp. 204-205.

4. Chand N. 2014. Diagnostic proceduresfor designated diseases of breedingbulls. In Training Manual: Production,processing and certification of qualitybovine semen, held at Central Institutefor Research on Cattle, Meerut from 18-25 November, 2014, pp. 154-164.

5. Chand N. 2014. Diseases transmittedthrough semen and their controlmeasures In Training Manual:Production, processing and certificationof quality bovine semen, held at CentralInstitute for Research on Cattle, Meerutfrom 18-25 November, 2014, pp. 102-112.

6. Chand N. 2014. Preparation of extenderfor dilution of semen. In Training Manual:Production, processing and certification



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of quality bovine semen, held at CentralInstitute for Research on Cattle, Meerutfrom 18-25 November, 2014, pp. 41-42.

7. Deb R and Sengar G. 2014. Methods forestimation of protein concentration. InTraining Manual: Molecular tools andbioinformatics approaches for livestockgenome analysis, held at CentralInstitute for Research on Cattle, Meerutfrom 10-30, September, 2014, pp. 221-222

8. Deb R, Gupta SK, Singh U and Sajjanar,B. 2014. Molecular basis for cellularthermotolerance induced by heat shockprotein among cattle. In Training Manual:Molecular tools and bioinformaticsapproaches for livestock genomeanalysis, held at Central Institute forResearch on Cattle, Meerut from 10-30,September, 2014, pp. 61-66.

9. Deb R, Raja TV, Singh U, Kumar S,Sengar G and Sharma S. 2014.Application of biotechnological tools forgenetic evaluation of breeding bulls. InTraining Manual: Production,Processing and certification of qualitybovine semen, held at Central Institutefor Research on Cattle, Meerut from 18-25 November, 2014, pp. 183-190.

10. Deb R, Sengar G and Singh R. 2014.Invitro amplification and its downstreamprocessing. In Training Manual:Molecular tools and bioinformaticsapproaches for livestock genomeanalysis, held at Central Institute forResearch on Cattle, Meerut from 10-30,September, 2014, pp. 166-170.

11. Deb R, Sengar G and Singh R. 2014.Molecular cloning and their applications.

In Training Manual: Molecular tools andbioinformatics approaches for livestockgenome analysis, held at CentralInstitute for Research on Cattle, Meerutfrom 10-30, September, 2014, pp. 178-183.

12. Deb R, Sharma S and Sengar G. 2014.Enzyme linked Immunosorbent Assayand their application. In Training Manual:Molecular tools and bioinformaticsapproaches for livestock genomeanalysis, held at Central Institute forResearch on Cattle, Meerut from 10-30,September, 2014, pp. 218-220.

13. Kumar M and Sharma A. 2014. Isolationand characterization of spematogonialstem cells. In Training Manual:Production, processing and certificationof quality bovine semen, held at CentralInstitute for Research on Cattle, Meerutfrom 18-25 November, 2014, pp. 197-202.

14. Kumar M. 2014. Maintenance andupkeep of cryo vessel-management andhandling of liquid nitrogen. In TrainingManual: Production, Processing andcertification of quality bovine semen,held at Central Institute for Research onCattle, Meerut from 18-25 November,2014, pp. 168-173.

15. Kumar M. 2014. Managing equipmentsand chemicals in semen freezinglaboratory. In Training Manual:Production, processing and certificationof quality bovine semen, held at CentralInstitute for Research on Cattle, Meerutfrom 18-25 November, 2014, pp. 15-21.

16. Kumar M. 2014. Sanitary proceduresfollowed at semen processing unit. In

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Training Manual: Production, processingand certification of quality bovinesemen, held at Central Institute forResearch on Cattle, Meerut from 18-25November, 2014, pp. 43-50.

17. Kumar R, Das AK , Singh U, Kumar S,Deb R and Raja TV. 2014.Biotechnological intervention in fieldprogeny testing for enhancement oflivestock productivity. In Training Manual:Molecular tools and bioinformaticsapproaches for livestock genomeanalysis, held at Central Institute forResearch on Cattle, Meerut from 10-30,September, 2014, pp. 48-51.

18. Kumar S and Kumar P. 2014. IntellectualProperty Rights (IPRs) and biosafetyGuidelines. In Training Manual:Molecular tools and bioinformaticsapproaches for livestock genomeanalysis, held at Central Institute forResearch on Cattle, Meerut from 10-30,September, 2014, pp. 229-234.

19. Kumar S, Alex R and Deb R. 2014.Development of Frieswal: Current statusand future prospects In Tyagi S, KumarM, Mandal DK et al., 2014. In TrainingManual: Production, Processing andcertification of quality bovine semen,held at Central Institute for Research onCattle, Meerut from 18-25 November,2014, pp. 123-128.

20. Kumar S, Deb R, Sharma S and SengarG. 2014. RNA isolation and cDNAsynthesis. In Training Manual: Moleculartools and bioinformatics approaches forlivestock genome analysis, held atCentral Institute for Research on Cattle,Meerut from 10-30, September, 2014,pp. 52-60.

21. Kumar S, Deb R, Sharma S and SengerG. 2014. RNA isolation and cDNAsynthesis. In Training Manual: Moleculartools and bioinformatics approaches forlivestock genome analysis, held atCentral Institute for Research on Cattle,Meerut from 10-30, September, 2014,pp. 183-190.

22. Mandal DK and Kumar M. 2014. SemenProcessing, Preservation and packagingof bovine semen. In Training Manual:Production, processing and certificationof quality bovine semen, held at CentralInstitute for Research on Cattle, Meerutfrom 18-25 November, 2014, pp. 82-89.

23. Mandal DK and Tyagi S. 2014.Andrological examination of breedingbulls. In Training Manual: Production,processing and certification of qualitybovine semen, held at Central Institutefor Research on Cattle, Meerut from 18-25 November, 2014, pp. 27-31.

24. Mandal DK. 2014. Assessment of sexualbehaviour in breeding bulls. In TrainingManual: Production, Processing andcertification of quality bovine semen,held at Central Institute for Research onCattle, Meerut from 18-25 November,2014, pp. 62-66.

25. Mathur AK. 2014. Certification of qualitybovine semen production unit as perCMU guidelines. Model Training Manual:Production, processing and certificationof quality bovine semen, held at CentralInstituteor Research on Cattle, Meerutfrom 18-25 November, 2014,pp. 203-204.

26. Mathur AK. 2014. Infertility in Frieswalcows. Training programme organized at



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Military Farm School & Centre, MeerutCantt., Meerut 4-9 August, 2014.

27. Pande M and Srivastava N. 2015.Routine semen evaluation and artificialinsemination. In Training Mannual:Reproductive Management: Anintegrated approach to augmentbreeding performance of Mithun andother livestock species, Held at ICAR-NRC on Mithun, Jharnapani,Medziphema, Nagaland from 2-11Feburary 2015 pp. 31-36.

28. Pande M, Srivastava N, Sharma A andSoni YK. 2014. Fertility markers inbovine semen. In Training Manual:Production, processing and certificationof quality bovine semen, held at CentralInstitute for Research on Cattle, Meerutfrom 18-25 November, 2014, pp. 174-182.

29. Prasad R, Singh P and Verma SK.2014. Nutritional interventions ofcrossbred bulls for optimum semenproduction. In Training Manual:Production, processing and certificationof quality bovine semen, held at CentralInstitute for Research on Cattle, Meerutfrom 18-25 November, 2014, pp. 113-117

30. Rai S, Kumar M, Tyagi S, Mandal DK,Srivastava N and Sirohi AS. 2014.CASA and alternative tools for assessingsperm quality. In Training Manual:Molecular tools and bioinformaticsapproaches for livestock genomeanalysis, held at Central Institute forResearch on Cattle, Meerut from 10-30,September, 2014, pp. 206-212.

31. Raja TV and Kumar R. 2014. ArtificialNeural Network and its application inAnimal Sciences. In Training Manual:Molecular tools and bioinformaticsapproaches for livestock genomeanalysis, held at Central Institute forResearch on Cattle, Meerut from 10-30,September, 2014, pp. 82-85.

32. Saha S, Sengar G, Deb R, Singh U andMathur AK. 2014. Somatic cell stainingand counting from milk samples.TrainingManual: Molecular tools andBioinformatics Approaches forLivestock Genome Analysis, heldatCentral Institute or Research on Cattle,Meerut from 10-30 September, 2014, pp.

33. Saha S, Kumar M and Mathur AK. 2014.Isolation and In-vitro culture of differenttypes of cells.Training Manual: Moleculartools and Bioinformatics Approaches forLivestock Genome Analysis, heldatCentral Institute or Research on Cattle,Meerut from 10-30 September, 2014, pp.213-217.

34. Singh C P, Pande M, Srivastava N,Sharma A, Kaushik S and Hemlata.2014. Different methods of semencollection. In Training Manual:Production, processing and certificationof quality bovine semen, held at CentralInstitute for Research on Cattle, Meerutfrom 18-25 November, 2014, pp. 36-40.

35. Singh CP, Pande M, Srivastava N,Sharma A, Kaushik S and Hemlata.2014. Semen collection using artificialvagina for harvesting quality semen frombulls. In Training Manual: Production,processing and certification of quality

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bovine semen, held at Central Institutefor Research on Cattle, Meerut from 18-25 November, 2014, pp. 52-55.

36. Singh P, Prasad R, Verma SK, Deb R,Das AK and Singh U. 2014.Nutragenomics and its application inlivestock production. In Training Manual:Molecular tools and bioinformaticsapproaches for livestock genomeanalysis, held at Central Institute forResearch on Cattle, Meerut from 10-30,September, 2014, pp. 52-60.

37. Singh U, Alex R, Deb R and Raja TV.2014. Advances in marker basedselection methodologies in animalbreeding. In Training Manual: Moleculartools and bioinformatics approaches forlivestock genome analysis, held atCentral Institute for Research on Cattle,Meerut from 10-30, September, 2014,pp. 28-33.

38. Sirohi AS and Kumar R. 2014.Maintenance of semen bank andstrategic shipment of frozen semenstraws. In Training Manual: Production,processing and certification of qualitybovine semen, held at Central Institutefor Research on Cattle, Meerut from 18-25 November, 2014, pp. 205-208.

39. Sirohi AS. 2014. Scientific action inanimal husbandry. Intermediate courseat MF School and Centre, Meerut Cantt.August 07.

40. Sirohi AS and Kumar R. 2014.Production, Processing and certificationof Quality bovine Semen In TrainingManual: Production, processing andcertification of quality bovine semen,held at Central Institute for Research on

Cattle, Meerut from 18-25 November,2014, pp. 205-208.

41. Sirohi AS, Mandal DK and Rai S. 2014.Bull management practices for qualitysemen production. In Training Manual:Production, processing and certificationof quality bovine semen, held at CentralInstitute for Research on Cattle, Meerutfrom 18-25 November, 2014, pp. 22-26.

42. Sirohi AS, Chand N and Srivastava N.2014. Alleviating stress in bulls foroptimum semen production. . In TrainingManual: Production, processing andcertification of quality bovine semen,held at Central Institute for Research onCattle, Meerut from 18-25 November,2014, pp. 148-153.

43. Soni YK, Mathur AK, Pande M andSrivastava N. 2014. Standard operatingprocedures for heat detection andartificial insemination techniques. InTraining Manual: Production, processingand certification of quality bovinesemen, held at Central Institute forResearch on Cattle, Meerut from 18-25November, 2014, pp. 191-196.

44. Srivastava N and Pande M. 2014.Recent advances in frozen sementechnology. In Training Manual:Production, processing and certificationof quality bovine semen, held at CentralInstitute for Research on Cattle, Meerutfrom 18-25 November, 2014, pp. 129-135.

45. Srivastava N, Pande M and Sharma A.2015. Semen collection and processing.In Training Mannual: ReproductiveManagement: An integrated approachto augment breeding performance of



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Mithun and other livestock species, heldat ICAR-NRC on Mithun, Jharnapani,Medziphema, Nagaland from 2-11Feburary 2015, pp. 21-30.

46. Srivastava N, Pande M, Soni YK,Kaushik S, Sharma A and Hemlata.2014. Routine evaluation of fresh semen.In Training Manual: Production,processing and certification of qualitybovine semen, held at Central Institutefor Research on Cattle, Meerut from 18-25 November, 2014, pp. 56-59.

47. Srivastava N, Pande M, Soni YK,Kaushik S, Sharma A and Hemlata.2014. Advancement in semen evaluationtechniques. In Training Manual:Production, processing and certificationof quality bovine semen, held at CentralInstitute for Research on Cattle, Meerutfrom 18-25 November, 2014, pp. 90-101.

48. Tyagi S and Mandal DK. 2014. Frozensemen technology: An overview. InTraining Manual: Production, processingand certification of quality bovinesemen, held at Central Institute forResearch on Cattle, Meerut from 18-25November, 2014, pp. 1-8.

49. Tyagi S. 2014. Endocrine control ofsemen production. In Training Manual:Production, processing and certificationof quality bovine semen, held at CentralInstitute for Research on Cattle, Meerutfrom 18-25 November, 2014, pp. 32-35.

50. Tyagi S. 2014. Infertility in male animals.In Training Manual: Production,processing and certification of qualitybovine semen, held at Central Institutefor Research on Cattle, Meerut from 18-25 November, 2014, pp. 60-61.

TECHNICAL/ POPULAR ARTICLES

1. Chakraborty D, Kumar D, Das AK, SinghM, Taggar RK., Kumar P and Kumar N.2014. Genetic Disorders in Cattle.Livestock Line June: 37-41

2. Singh R, Singh U and Deb R. 2014.Molecular Insight into Integrin Alpha Beta6 Receptor of Cattle and theirRelationship with FMD Infectivity.Journal of Cell Science & MolecularBiology 1(2): 1-2.

PAPERS PRESENTED IN CONFER-ENCES/SYMPOSIA/WORKSHOP

1. Chand N, Dhaliwal PS and Uppal SK.2015. Therapeutic management of bloodtransfusion reaction in crossbred cow.Annual Convention of Indian Society forVeterinary Medicine held at KVASU,Pookode, Wayanad, Kerala. January,22-24. 2015. pp-278.

2. Kumar J, Singh YP, Singh R, Rajkumarand Kumar R. 2015. Genetic Analysis ofProductive and ReproductivePerformance of Frieswal cattle at Militryfarm Ambala. International Symposiumon Sustainable management of AnimalGenetic Resources for LivelihoodSecurity in Developing Countries held atMadras Veterinary College, Chennai.February, 13-14, 2015. pp-53.

3. Kumar R, Das AK, Rathee SK, KumarA, Mandal DK, Singh U, Raja TV, KumarS, Alex R, Kumar AK, Singh CB, DubeyPP and Gokhale SB. 2015. Geneticimprovement of crossbred cattle throughfield progeny testing. InternationalSymposium on Sustainablemanagement of Animal Genetic

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Resources for Livelihood Security inDeveloping Countries held at MadrasVeterinary College, Chennai. February,13-14, 2015. pp-248.

4. Kumar S, Alex R, Singh U, Deb R,Prakash B and Baswana J. 2015.Lactational Performance Evaluation ofFrieswal cows in Northern Zone of India.International Symposium on Sustainablemanagement of Animal GeneticResources for Livelihood Security inDeveloping Countries held at MadrasVeterinary College, Chennai, Tamil Nadufrom February, 13-14. 2015. pp-52.

5. Mathur AK and Prasad R. 2015.Reproductive performance ofpostpartum Frieswal cows fedindividually under farm conditions. 102nd

Indian science congress, session:animal veterinary & fishery science heldat Mumbai University, Mumbai. January03-07, 2015. pp. 254.

6. Pande M, Rajoriya JS, Ghosh SK,Prasad JK, Srivastava N, Das GK, SoniYK, Ramteke SS and Mathur AK. 2014.Effects of degasified extender on qualityparameters of cryopreserved bullspermatozoa. International symposiumon current challenges and translationalresearch to augment animal reproductionheld at MVC Chennai, December 4-5,2014. pp-193.

7. Prasad R, Mishra, AS and Girdhar N.2014. Soil-water-plant-animalinterrelationship of micro minerals inMeerut district of Uttar Pradesh. 102Indian Science Congress held atUniversity of Mumbai, January 3-7, 2015.pp-179.

8. Prasad R, Verma SK, Mishra AS andGirdhar N. 2014. Effect of plane ofnutrition on growth and performance ofFrieswal bulls. Global Animal NutritionConference held at Vivanta by Taj -Yashwanthpur, Bengaluru, April 20-22,2014. pp-225.

9. Raja TV, Gandhi RS, Singh U, PrakashB, Kumar S and Deb R. 2015. EmpiricalApplication of Response SurfaceMethodology for increasing the lifetimemilk yield through optimizing theproduction and reproduction traits inSahiwal cattle. International Symposiumon Sustainable management of AnimalGenetic Resources for LivelihoodSecurity in Developing Countries held atMadras Veterinary College, Chennai,Tamil Nadu from February, 13-14. 2015.pp-248.

10. Rajeev, Kumar R, Singh R, Singh H,Chauhan, Kumar P and Singh NK. 2015.Sustainable feeding and managementpractices by cattle’s keepers in westernUttar Pradesh. International Symposiumon Sustainable management of AnimalGenetic Resources for LivelihoodSecurity in Developing Countries held atMadras Veterinary College, Chennai.February, 13-14, 2015. pp-200.

11. Singh G, Randhawa SNS, Chand N andNayar S. 2015. Metabolic profiling ofcrossbred dairy cows duringperiparturient period. Annual Conventionof Indian Society for Veterinary Medicineheld at KVASU, Pookode, Wayanad,Kerala. January, 22-24, 2015. pp-282-83.

12. Singh G, Randhawa SNS, Chand N andRandhawa CS. 2015. Therapeutic



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management of crossbred dairy cowsaffected with subclinical ketosis. AnnualConvention of Indian Society forVeterinary Medicine held at KVASU,Pookode, Wayanad, Kerala. January,22-24, 2015. pp-283-84.

13. Singh I, Smita S, Kumar R, Deb R,Jerome, Murali S, Chandran R,Radhakrishnan NV, Sahoo NR, MishraD C, Rai A and Kumar S. 2015. In silicostudies deciphering Foot- and Mouthdisease (FMD) virus-host tropism.Symposium on Accelerating Biology2015: catalyzing evolution held at thebioinformatics group of C-DAC, Pune,January 20-22, 2015. pp-130-131.

14. Singh R, Deb R, Singh U, Alex R,Alytheodi RR, Kumar S, Sharma S andSengar G. 2015. Single tube tetra primerbased detection of novel SNPs at 5’ –UTR and exon coding region of bovineITGB6 receptor gene and theirassociation with foot and mouth diseasesusceptibility in cattle, XII AgricultureScience Congress held at ICR-NDRI,Karnal from February 03-06, 2015. pp-178.

15. Singh U, Raja TV, Alyethodi RR, RathodBS, Tomar AK, Patel JB and Prakash B.2015. Genetic Improvement of KankrejCattle through Associated Herd ProgenyTesting Programme. International

Symposium on Sustainablemanagement of Animal GeneticResources for Livelihood Security inDeveloping Countries held at MadrasVeterinary College, Chennai, Tamil Nadufrom February, 13-14, 2015. pp-247.

16. Soni YK, Mathur AK, Pande M, Tyagi S,Mandal DK, Prasad R and Verma S K.2014. Status of reproductive disordersin bovines of rural areas aroundMeerut.XXX Annual convention ofISSAR & National symposium onresearch and innovations to improveanimal fertility & fecundity held atDUVASU, Mathura. November 20-22,2014. pp. 225.

17. Srivastava N, Srivastava SK, Ghosh SK,Kumar Amit, Perumal P, Pande M andSoni YK. 2014. Studies on sequestrationof PDC-109 on cryoinjury of crossbredbull spermatozoa. Internationalsymposium on current challenges andtranslational research to augment animalreproduction held at MVC Chennai,December 4-5, 2014. pp-192.

18. Verma SK, Prasad R and Girdhar N.2014. Effect of plane of nutrition on milkurea nitrogen content in Frieswal cattle.Global Animal Nutrition Conference heldat Vivanta by Taj - Yashwanthpur,Bengaluru, April 20-22, 2014. pp-281.

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TRAINING/CONFERENCE/SEMINAR/WORKSHOPATTENDED

Scientific staff

S. Name of events Venue/Period Name of theNo. Scientist

1 Global Animal Nutrition Conference 2014 organized NIANP, Bengaluru Dr. R Prasadby Animal Nutrition Society of India April 20-22, 2014 Dr. SK Verma

2 Brain Storming Session on “Productivity improvement U.P. Council of Agricultural Dr. AK Dasthrough livestock management” Research, Lucknow, Dr. Rajib Deb

May 07 ,2014

3 Application of Electronics in Agriculture and CDAC, Kolkata Dr. M KumarEnvironment June 16-27, 2014

4 Training Programme on Organic Farming Technique Village Bhagwanpur, Dr. SK Verma& Practices organized by Jan Kalyan Sanstha, Block Mawana, Distt. Dr. P SinghMeerut and sponsored by NABARD, Meeut, Meerut

June 20, 2014

5 Refresher Course on Agricultural Research National Academy of Dr. AK DasManagement for Directly Recruited Senior / Agricultural ResearchPrincipal Scientist Management, Hyderabad.

July 14-26, 2014

6 Management Development Programme on ICAR-NAARM, Dr. S TyagiLeadership Development (a pre-RMP programme) Hyderabad Dr. S Kumar

July 15-26, 2014

7 Workshop on National Training Policy Institute of Secretarial Dr. DK MandalTraining and Management,New DelhiAugust 04-05, 2014

8 Harnessing intellectual property in animal science NIANP, Bangalore Dr. AS Sirohisector in the changing global scenario August 18-27, 2014

9 AICRP Annual Review Meet GBPUAT, Pantnagar. Dr. AK DasAugust 29, 2014 Dr. Umesh Singh

Dr. S Kumar

10 XXII Regional Committee IV Meeting ICAR-IISR, Lucknow, Dr. B PrakashSeptember 6-7, 2014

11 DBT Sponsored Short Term Training Course on ICAR-CIRC, Meerut, Dr. Megha Pande“Molecular Tools and Bioinformatics Approaches for September 10- 30, 2014 Dr. YK SoniLivestock Genome Analysis”

12 2nd International Conference on Animal & Dairy OMICS Group Dr. AS SirohiSciences Conference, Hyderabad, Dr. Rajib Deb

September 15-17, 2014



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13 Training on “Knowledge Management & Knowledge Indian Institute of Public Dr. S SahaSharing in Organization” Administration (IIPA),

New Delhi.One week from September22- 26, 2014

14 Silver Jubilee convention of ISAPM of National Navsari Agri. Univ. Navsari, Dr. AK MathurSeminar of revisiting management policies and October 9-11, 2014. Dr. DK Mandalpractices for indigenous livestock and poultry breedsas eco-friendly economic producers.

15 ICAR –NAVS Expert Consultation Meet on NASC Complex, New Delhi, Dr. B PrakashStrategies for enhancing milk productivity of October 20, 2014 Dr. AK MathurIndigenous cattle Dr. R Prasad

Dr. S TyagiDr. Umesh SinghDr. Sushil KumarDr. Raja TVDr. Ravinder Kumar

16 Training on “Advanced Techno-Management Administrative Staff Dr. S SahaProgramme for Scientists” College of India (ASCI), Dr. P Singh

Hyderabad,Five weeks from October27 to November 28, 2014

17 Advanced concepts and techniques to augment National Institute of Dr. RR Alyethodireproduction in livestock Animal Nutrition and

Physiology (NIANP),Adugodi, BengaluruNovember 12 toDecember 2 , 2014

18 XXX Annual convention of ISSAR & National DUVASU, Mathura Dr. YK Sonisymposium on “Research and Innovations to improve November 20-22, 2014animal fertility & fecundity”

19 National Congress on Veterinary public health and XIth NASC Complex, Dr. AK Mathurconference of association of public health veterinarians: New Delhi.present status and future road map. November 24-25, 2014.

20 Management Development Programme on Leadership NAARM Hyderabad, Dr. Umesh SinghDevelopment (A pre-RMP programme) December 01-12, 2014

21 Short course training on “Advanced Molecular and NBAGR, Karnal, Dr. Ravinder KumarBioinformatics Approaches for Genome Characterization December 01-10, 2014of Indigenous Animal Genetic Resources”

22 International Symposium on Current Challenges and Madras Veterinary College, Dr. Megha PandeTranslational Research to Augment Animal December 4-5, 2014 Dr. N SrivastavaReproduction

23 ICAR CAFT training program on Advances in Omics ICAR- IASRI, New Delhi, Dr. Rajib DebData Analysis: Learning by Example December 03-23,2014

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24 fgUnh dk;Z'kkyk dsUnzh; vkyw vuqla/kku MkW- latho dqekjlaLFkku] eksnhuqje] esjB oekZDecember 20, 2014

25 102 Indian Science Congress University of Mumbai, Dr. AK MathurJanuary 3-7, 2015 Dr. R Prasad

26 Executive Development Programme on Leadership ICAR-NAARM, Hyderabad, Dr. B PrakashDevelopment January 10, 2015

27 33rd ISVM Annual Convention & National Symposium Kerala Veterinary and Dr. Naimi ChandAnimal Sciences University,Pookode, Wayanad,January 22-24, 2015

28 ISAPM National Seminar Anand Agri. Univ. Anand, Dr. AK Mathur28-30 Jan 2015 Dr. DK Mandal

29 XII Agriculture Science Congress ICR-NDRI, Karnal Dr. B PrakashFebruary 03-06, 2015 Dr. S Tyagi

Dr. Umesh SinghDr. SK VermaDr. YK Soni

30 International Symposium on Sustainable Management Madras Veterinary College, Dr. B Prakashof Animal Genetic Resources for Livelihood Security Chennai, Dr. Umesh SinghIn Developing Countries & XII Annual Convention of February 13-14, 2015 Dr. Ravinder KumarSociety for Conservation of Domestic Animal Biodiversity Dr. Raja TV

31 Village Seminar during Field Experience Training of Village Pooth Khas, Dr. S. TyagiARS Probationers Block Rohta, Distt. Meerut Dr. SK Verma

March 4, 2015 Dr. Naimi Chand

32 ICAR-INRA Joint Workshop NASC Complex, New Delhi, Dr. Umesh SinghMarch 10, 2015

33 Advanced tools for analysis of Phenomic and National Dairy Research Dr. Rani AlexGenomic data Institute (NDRI), Karnal

March 5-25, 2015

Administrative Staff

S. Name of events Venue/Period Name of theNo. Officer/official

1 Workshop on CSP for Assistants (DR) of ICAR ISTM, New Delhi Shri SL Gautam,May 19-30, 2014 Assistant

2 Workshop on Income Tax (WITac-02) for the DDOs ISTM, New Delhi Shri OP Agarwal,and dealing Assistants July 07-08, 2014 Assistant

3 Workshop on CSP for Assistants of ICAR ISTM, New Delhi Shri Manoj Nehra,January 19-21, 2015 UDC

4 Workshop on Communication Skills ISTM, New Delhi Smt. Anita Jain,January 19-20, 2015 Private Secretary

5 Orientation Course in Records Management for National Archives of Shri DS Verma,Record Officers India, New Delhi AF&AO

Feburary 09-11, 2015



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SEMINAR ORGANIZED/TRAININGS IMPARTED

S. Name of the training/seminar Venue and date CourseNo. Director/

OrganizingSecretary

1 XIII Annual Review Meet of AICRP on Cattle GBPUA&T, Pantnagar Dr. Umesh SinghAugust 29, 2014 Dr. Sushil Kumar

Dr. AK Das

2 DBT sponsored Training entitled “Molecular Tools and ICAR-CIRC, Meerut Course Director:Bioinformatics Approaches for Livestock Genome” September 10-30, 2014 Dr. Umesh Singh,

Course Co-organizer:Dr. Sushil KumarDr. Rajib Deb

3 Model Training Course on “Production, Processing ICAR-CIRC, Meerut Course Director:and Certification of Quality Bovine Semen” Cantt Dr. S Tyagi

November 18–25, 2014 Course Co-organizer:Dr. M KumarDr. DK MandalDr. AS SirohiDr. N SrivastavaDr. Saroj Rai

4 Field Experience Training (FET) to the 101st batch ICAR-CIRC, Meerut Course Director:of ARS probationers (FOCARS) from NAARM, Cantt Dr. S TyagiHyderabad February19 –March 11, Dr. SK Verma

2015

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PROFESSIONAL RECOGNITIONS

● Dr. Rajib Deb acted as organizingcommittee member in 2nd InternationalConference on Animal & Dairy Sciencesheld during September 15-17, 2014 atHyderabad, India hosted by OMICSGroup Conferences.

● Dr Megha Pande has been appointed asa Reviewer of a scientific journal viz.International Journal of VeterinaryScience Research from February 2015.

Awards

❖ First best poster presentation award forSingh U, Raja TV, Alyethodi RR, RathodBS, Tomar A K, Patel JB and Prakash B.2015. Genetic Improvement of KankrejCattle through Associated Herd ProgenyTesting Programme in InternationalSymposium on International Symposiumon Sustainable Management of AnimalGenetic Resources for Livelihood

Security in Developing Counties and XIIAnnual Convention of Society forConservation of Domestic AnimalBiodiversity (SOCDAB) held at MadrasVeterinary College, Chennai, Tamil Nadufrom Feb 13-14, 2015.

❖ Second best poster presentation awardfor Jeichitra V, Rajendran R, Raja T V,Karunanithi K and Rahumathulla P S.2015. Genetic parameter estimates forgrowth traits in Mecheri sheep usingrandom regression animal models inInternational Symposium on InternationalSymposium on Sustainable Managementof Animal Genetic Resources forLivelihood Security in DevelopingCounties and XII Annual Convention ofSociety for Conservation of DomesticAnimal Biodiversity (SOCDAB) held atMadras Veterinary College, Chennai,Tamil Nadu from Feb 13-14, 2015.

INSTITUTE TECHNOLOGY MANAGEMENT UNIT(ITMU)

During this year 2014-15, ITMU arrangedmeeting of Institute Technology ManagementCommittee on March 23, 2015 under thechairmanship of Dr. Birham Prakash fordiscussion of some patent issues andfunctioning of Institute TechnologyManagement Unit. A seminar was deliveredfor scientists and staff on ICAR Guidelinesfor Internal Evaluation & ForwardingResearch Papers to Scientific Journals &Data Management. A Lectures on

Intellectual Property Rights (IPRs) andbiosafety guidelines was also delivered inDBT sponsored training program held atICAR-CIRC campus.

Students, farmers and other para-vetvisiting the institute were given on latesttechnology information developed by theinstitute and other ICAR institute for livestock.ITMU facilitated the scientists in prior artsearch and patent registration and filing of



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patents. During the year two design patentswere registered and one process patent wasready for provisional submission. ITMU alsoassisted in event management i.e. collectinginformation and photographs of differentevents being organized at the institute and

also provided photographs for institutewebsite.

ITMU also assisted in compilation ofAnnual reports and provided technical helpfor online tender uploading.

RIGHT TO INFORMATION ACT 2005

IMPORTANT COMMITTEES

RESEARCH ADVISORY COMMITTEE (RAC)

1. Dr. Sushil Kumar ChairmanFlat No. 402, New Anand Apartments,Plot No. 47, Sector-56,Gurgaon (Haryana)

2. Dr. OS Parmar MemberFormer Director (Research), GADVASU,H.No. 319/B, Bhai Randir Singh Nagar,Ludhiana (Punjab)

3. Dr. SBS Yadav MemberProfessor of Animal Breeding,CVAS Rajasthan Agricultural University,Bikaner (Rajasthan)

4. Dr. SL Goswami MemberDirector,ICAR-National Academy of Agril. Research ManagementRajendra Nagar, Hyderabad (AP)

5. Dr. MC Handa Member42, New Rajguru Nagar,Ludhiana (Punjab)

A total of 13 applications were receivedfrom different places to seek informationunder the Right to Information Act, 2005during the year 2014-15 (April 2014-March2015) for providing the information. The

desired information was provided to allinformation seekers within stipulated time. Atotal of Rs. 140/- (hundred forty only) wasreceived as application and others charges.

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6. Dr. ML Maheshwari MemberEx-Head, Veterinary College,Abhilasha, 9, Civil Lines, Mathura (U.P.)

7. Dr. NG Hegde MemberTrustee and Principal Adviser,BAIF Development Research Foundation,Dr. Manibhai Desai Nagar, N.H. No.4, Warje,Pune (Maharashtra)

8. Director, MemberICAR-Central Institute for Research on Cattle,Meerut Cantt. (U.P.)

9. Asstt. Director General (AP&B) MemberIndian Council of Agricultural Research,Krishi Bhawan, New Delhi

10. Shri Zafar Akhtar Member144, B.C. Lines,Meerut Cantt. (U.P.)

11. Shri Nitin Kumar Tyagi MemberVill. & P.O. Rasna,Distt. Meerut (U.P.)

12. Dr. AK Mathur Member SecretaryPrincipal Scientist,ICAR-Central Institute for Research on Cattle,Meerut Cantt. (UP)

INSTITUTE MANAGEMENT COMMITTEE (w.e.f 7.8.2013)

1. Director ChairmanICAR-Central Institute for Research on Cattle,Meerut (UP)

2. The Chief Executive Officer MemberUP Livestock Development BoardDirectorate of Animal Husbandry CampusGorakhnath Road, Badshah Bagh,Lucknow-226007 (UP)



82

3. Dr. RS Patel MemberManaging Director,Amroli Distt. Milk Cooperation Ltd.,Amroli (Gujarat)

4. Dr. Rajvir Singh Dean, MemberCOVAS, SVBPUAT,Meerut (UP)

5. Dr. AK Pandey MemberPrincipal Scientist (AG&B),ICAR-Central Institute for Research on Buffaloes,Hisar (Haryana)

6. Dr. Avtar Singh MemberPrincipal Scientist (AG&B),ICAR-National Dairy Research Institute,Karnal (Haryana)

7. Dr. RK Pundir MemberPrincipal Scientist (AG&B),ICAR-National Bureau of Animal Genetics Research,Karnal (Haryana)

8. Dr. Umesh Singh MemberPrincipal Scientist (AG&B),ICAR-Central Institute for Research on Cattle,Meerut (UP)

9. Shri BK Bisht MemberSr. Finance & Accounts Officer,ICAR-NBPGR, New Delhi

10. Dr. Vijay Kumar Pandit MemberChief Functionary,Green Care Society,57, Vikas Enclave, Rohta Road,Meerut (U.P.)

11. Shri Vinod Bharti MemberVillage Arnawali, Block Rohta,Meerut (U.P.)

12. Shri Rishi Ram Member SecretaryAdministrative OfficerICAR-Central Institute for Research on Cattle,Meerut (UP)

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INSTITUTE JOINT STAFF COUNCIL (IJSC)

Office side

1. Dr. AK Mathur (upto 15/8/2014) : Chairman

Dr. Birham Prakash, Director (w.e.f. 16/8/2014) : Chairman

2. Dr. Sushil Kumar, Principal Scientist : Member

3. Shri Rishi Ram, AO & H.O. : Member

4. Shri DS Verma, AF&AO : Member

5. Smt. Anita Jain, Private Secretary : Secretary

Staff side

1. Shri NS Saini, Assistant : Member

2. Shri Vikas Kumar, LDC : Member

3. Shri SK Sharma, Technical Officer : Secretary

4. Shri Omkar Singh, Sr.Technical Assistant : Member

5. Shri Jitendra Giri, Skilled Supporting Staff : Member

6. Shri Sunil Kumar, Skilled Supporting Staff : Member (CJSC)



84

IMPORTANT MEETINGS

Institute Animal Ethics Committee (IAEC)

The meeting of IAEC was held on 2nd

August, 2014 and 13 new researchproposals from the scientist of the Institutewere discussed and approved as per thenorms of CPCSEA.

It was also suggested to discuss all thenew project proposals in detail in the IRCparticularly with respect to their technicalprogramme. In the project related todevelopment of fertility score card of bulls,RAC suggested that parameters for in-vitroassessment of fertility should also be takenup. For nutritional experiments, the nutritionalstatus of the animals should be known to theresearchers and nutritional experimentsshould be carried out under the ownsupervision of the concerned researchworkers. The latest NRC recommendationsshould be considered while taking up thenutrition projects. The RAC also advised thata control group should invariably be taken upunder any nutritional experiments. It was alsosuggested that Dr. Rajib Deb presentlyworking in AG&B section should be entrustedwith the research work related to health,microbiological and disease diagnosticaspects because of his specialisation on thatparticular area of biotechnology.

Institute Research Council (IRC)

The Institute Research Committee (IRC)meeting for the year 2013-14 was held on

Research Advisory Committee (RAC)

Meeting of XIV RAC was conducted on28 May, 2014 at CIRC, Meerut campus. RACstressed upon achieving the target of 4000litre milk yield per lactation in Frieswal cowsas per objective of the Frieswal project. It wasalso suggested to include some of theindigenous breeds like Khilari under theIndigenous breeds project for study ondraught purpose and making availability ofsemen doses of some of the importantindigenous breeds such as Sahiwal, Kankrej,Gir etc. at CIRC for meeting the demand oflocal farmers. The RAC also expressed itsconcern about stagnant performance of BAIFFPT Unit and extremely low conception rate(31%) at field conditions and recommendedclose monitoring of the unit for improving itsperformance.

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12.09.2014 under the chairmanship of Dr B.Prakash, Director, CIRC. At the outset, thechairman impressed upon to follow theCouncil guidelines of involvement of theScientists in research projects in 1+2 fashion(1 as PI and 2 as Co-PI). The annual progressof all the three long term AICRP, 5 on-goingprojects including one DST sponsored andfinal report of 2 completed projects wasdiscussed. The concluding reports ofresearch projects on "Characterization anddifferentiation of embryonic andspermatogonial stem cells in cattle andbuffaloes" and "Genetic basis of inferiorsperm quality and fertility of crossbred bulls"sponsored by NAIP were also presented byrespective PIs. A total of 18 new researchproposals on various aspects related withbreeding, genetics, nutrition, reproductionand management were also placed beforethe IRC, however, 4 projects were droppedafter discussion.

Annual Review Meeting of AICRP

The XIII Annual Review Meeting of AICRPon Cattle was held on August 29, 2014 atGovind Ballabh Pant University of Agricultureand Technology, Pantnagar (Uttrakhand)under the chairmanship of Dr. K. M. L Pathak,DDG (AS), ICAR, New Delhi. Chairmanstressed upon taking necessary steps toimprove the performance of the projects andalso for close monitoring of the on-goingresearch projects so that desired progresscould be achieved in the existing and newprogrammes approved in the XII plan. Hedesired that all the units should maintain thecomputerized data base and ensure transferof data to coordinating institute on regularbasis. He asked PIs of all Coordinating unitsof AICRP on Cattle to monitor the projectintensively by frequent visits to units. Whilechairing the session he stressed that SAU/Veterinary Universities must assure budgetutilization and release of 25% contribution ascommitted in the MOU to achieve the target.The meeting was also attended by PIs ofvarious FPT centers located in different partof the country. Dr. B. Prakash, Director, CIRC;Dr. R. S. Gandhi, ADG (AP&B), ICAR, NewDelhi; Dr. J. P. S. Gautam, Director Research,GBPUA&T, Pantnagar and Dr. J. Kumar,Officiating Vice Chancellor GBPUA&T,Pantnagar also gave their valuable inputs inthe scientific deliberations.



86

MONTHLY SEMINAR

Sl. Title Name of speaker DateNo.

1 Increasing Cattle Productivity Using Latest Dr. AK Das, Principal Scientist 30-04-2014Breeding Tools

2 CLA: Food Enrichment Through Animal Feeding Dr. Pramod Singh, Principal 29-05-2014Scientist

3 Financial Rules Sh. Dharmender Verma, A F & A O 17-06-2014

4 Understanding Thermoregulation Mechanism Dr. AS Sirohi, Senior Scientist 25-07-2014in Bovines

5 ETT as a tool of faster multiplication of Dr. Ravinder Kumar, Senior 26-08-2014germplasm Scientist

6 Development of a Chimeric DNA Vaccine Dr. Rajib Deb, Scientist 25-09-2014Construct Against Infectious Bursal Diseaseof Poultry

7 Concepts and application of Artificial Neural Dr. TV Raja, Senior Scientist 29-10-2014Network in Animal Sciences

8 Extragonadal Life of Bovine Spermatozoa in Dr. N Srivastava, Senior Scientist 27-11-2014Relation to BSP Proteins

9 ICAR Guidelines for Internal Evaluation & Dr. Sushil Kumar, Principal 20-12-2014Forwarding Research Papers to Scientific ScientistJournals & Data Management

10 Role of Vigilance in ICAR Institutes Dr. Umesh Singh, Principal 31-01-2015Scientist

11 HRD: Development of training policy in ICAR Dr. Pramod Singh, Principal 28-02-2015Scientist

INSTITUTE ACTIVITIES

Celebrations in the Institute

Independence Day Celebration

The 68th Independence day of the Nationwas celebrated on 15th August 2014. Dr. A.K.Mathur, Acting Director, CIRC hoisted theNational Flag followed by recitation ofNational Anthem. During his IndependenceDay speech he remembered all those who

87



sacrificed their lives for this dream come trueand paid tribute to them. On the occasion Dr.Mathur congratulated the staff and theirfamilies for this auspicious day of the countryand invoked the staff to work in disciplinedand dedicated way for the cause of cattleresearch and development in the country. Healso emphasized that the staff has todedicatedly work for the Institute which in turnadd to the cattle development and prosperityof the country.

Republic Day Celebration

The 66th republic day was celebrated on26th January, 2015. Dr. Birham Prakash,Director CIRC hoisted the National Flagfollowed by recitation of National Anthem bythe staff. On the occasion Dr. Prakashextended hearty congratulation to the staff and

FIELD EXPERIENCE TRAINING (FET) OFARS PROBATIONERS

NAARM, Hyderabad has recognizedICAR-CIRC as FET Centre for ARSprobationers. Six ARS probationers of 101st

batch joined ICAR-CIRC for FieldExperience Training from 19.02.2015 to11.03.2015. Dr. Shrikant Tyagi, PrincipalScientist and Dr. Sanjeev Kumar Verma,Senior Scientist coordinated the trainingprogramme. During the FET, Village PoothKhas was selected as target area and theprobationers conducted exhaustive surveyusing PRA technique and identified keyproblems being faced by the villagers andfarmers. Remedial measures for variousproblems were suggested to the farmersduring village seminar on 10th March, 2015.The ARS probationers delivered InstituteSeminar on 4th March, 2015 during whichfamilies who attended the ceremony and

apprised the staff about ongoing activitiesand future targets of the Institute going to beundertaken during remaining years of the XIIPlan. He emphasized the need to workdedicatedly for the welfare of the Institute aswell as country. He cited the initiatives likeSwachh Bharat Abhiyan undertaken by thegovernment and urged the staff to participatewhole heartedly. On the occasion the staffclub organized get together for staff families.A quiz competition for the children and fungame etc. were organized.

ARS probationers delivering Institute seminarat ICAR-CIRC, Meerut



88

visiting faculty from NAARM was alsopresent. During this training, probationersvisited Modi Sugar Mills, Modinagar, Meerut,Uttar Pradesh; Parag Cattle Feed Plant,Partapur, Meerut, Uttar Pradesh and GangolSahkari Dugdh Utpadak Sangh Limited(Parag Diary), Partapur, Meerut, UttarPradesh. All the 6 probationers successfullycompleted their training.

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Village seminar at Village Pooth Khas, Meerut

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90

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92

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93



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Foundation day celebration

The 28th foundation day of CIRC wascelebrated on 3.11.2014 by hosting academicand cultural events. At the outset of the

function Dr. Birham Prakash, Director, CIRC,gave the glimpses of the scientific activitiesundertaken during past 27 years,achievements made and the future goals ofthe institute. On this occasion sport events

like hammer throw, discus throw, musicalchair etc. were organized and the winnerswere given prizes. The CIRC staff took partwith enthusiasm in various activitiesorganized on the day.



94

ACTIVITIES OF STAFF CLUB

Book fair organized

Two book fairs were organized at the

Institute on 28th May, 2014 and 3rd March,2015 to acquaint the researchers andstudents about the new publications availablein the market.

DISTINGUISHED VISITORS

Visit of dignitaries to the Institute

Subarea commander visited institute:Maj. Gen. Sunil Yadav, YSM, GOC PaschimUP Sub area visited the Institute on 7th July2014. He was accompanied by Col. JagjitBaswana, Director MF&FP and other officersof Military Farm, Meerut. General Yadav tooknote of the activities undertaken by theInstitute with Military Farms and visited variouslaboratories and interacted with the scientistsof the Institute. He applauded the activities ofInstitute and the collaborative work beingtaken with Military Farms and assured of fullcooperation in future.

QMG visited the Institute: On 18th

December 2014 QMG visited differentlaboratories of the Institute and getacquainted with the research work beingcarried out here.

Uttar Bharat Sub Area commandervisited the Institute: Lt. GeneralVishwambhar Singh, VSM, GOC UB areaaccompanied by Major General Sunil YadavGOC, Meerut Sub area visited the Instituteon 14th February, 2015. General Singhinteracted with the scientists and MilitaryFarm Officers. In the meeting Col. JagjitBaswana, Director Frieswal appraised the

95



collaborative working of Frieswal Project. TheLt. General was also explained about theother projects running at the Institute by thescientists. Subsequently Lt. General Singhvisited the laboratories and semen collectionarea where he expressed happiness aboutthe working of Frieswal project.



96

STAFF POSITION

SCIENTIFIC

Sl. Name of officer Designation (Discipline)No.

1. Dr. Birham Prakash Director (w.e.f. 16.8.14)

2. Dr. A.K. Mathur Principal Scientist (Animal Physiology)(Acting Director, w.e.f. 18.12.2013 to 15.8.14)

3. Dr. Rajendra Prasad Principal Scientist (Animal Nutrition)

4. Dr. Shrikant Tyagi Principal Scientist (Animal Physiology)

5. Dr. Umesh Singh Principal Scientist (Animal Genetics & Breeding)

6. Dr. Sushil Kumar Principal Scientist (Animal Genetics & Breeding)

7. Dr. Mahesh Kumar Principal Scientist (Animal Physiology)

8. Dr. A.K. Das Principal Scientist (Animal Genetics & Breeding)

9. Dr. Pramod Singh Principal Scientist (Animal Nutrition)

10. Dr. D.K. Mandal Sr. Scientist (Livestock Production & Management)

11. Dr. S.K. Verma Sr. Scientist (Animal Nutrition)

12. Dr. Ajaveer Singh Sirohi Sr. Scientist (Livestock Production & Management)

13. Dr. Ravinder Kumar Sr. Scientist (Animal Genetics & Breeding)

14. Dr. T.V. Raja Sr. Scientist (Animal Genetics & Breeding)

15. Dr. Neeraj Shrivastava Sr.Scientist (Animal Reproduction & Gynaecology)

16. Dr. Siddhartha Saha Sr. Scientist (Animal Physiology)

17. Dr. Naimi Chand Sr. Scientist (Veterinary Medicine)

18. Shri S.K. Rathee Scientist (Animal Genetics & Breeding)

19. Dr. (Mrs.) Saroj Rai Scientist (Livestock Production & Management)

20. Dr. Rajib Deb Scientist (Animal Biotechnology)

21. Dr. R.R. Alyethody Scientist (Animal Genetics & Breeding)

22. Dr. Rani Alex Scientist (Animal Genetics & Breeding)

23. Dr. (Mrs.) Megha Pande Scientist (Animal Reproduction & Gynaecology)

24. Dr. Yogesh Kumar Soni Scientist (Animal Reproduction & Gynaecology)

TECHNICAL

1. Dr. L.S. Arya Chief Technical Officer

2. Shri C.P.Singh Chief Technical Officer

97



3. Shri Rajiv Verma Sr. Technical Officer

4. Shri Y.P. Malhotra Technical Officer

5. Shri S.K. Sharma Technical Officer

6. Shri Suresh Chand Technical Officer

7. Shri Omkar Singh Sr. Technical Assistant

8. Shri Chhote Singh Sr. Technical Assistant

ADMINISTRATIVE

1 Shri Rishi Ram Administrative Officer

2 Shri D.S. Verma Asstt. Finance & Account Officer

3 Shri A.K. Sharma Asstt. Administrative Officer

4 Smt. Anita Jain Private Secretary

5 Shri N.S. Saini Assistant

6 Shri S.L. Gautam Assistant

7 Shri O.P. Aggarwal Assistant

8 Shri P.K. Gautam Assistant

9 Smt. Pushpa Personal Assistant

10 Shri Shankar Kashyap Stenographer

11 Shri Manoj Nehra Upper Divisional Clerk

12 Shri A.K. Tripathi Lower Divisional Clerk

13 Shri Vikas Kumar Lower Divisional Clerk

SUPPORTING

1 Shri Veer Mahendra Skilled Supporting Staff

2 Shri Kailash Skilled Supporting Staff

3 Shri Dungar Singh Skilled Supporting Staff

4 Shri Siyanand Skilled Supporting Staff

5 Shri Jitendra Giri Skilled Supporting Staff

6 Shri Mohan Chandra Skilled Supporting Staff

7 Shri Umesh Kaushik Skilled Supporting Staff

8 Shri Veer Pal Singh Skilled Supporting Staff

9 Shri Sunil Kumar Skilled Supporting Staff

10 Mrs. Neerja Joshi Skilled Supporting Staff



98

PERSONNEL MILESTONES

PROMOTIONS

● Shri Yash Pal Malhotra, Sr. TechnicalAssistant promoted to the post ofTechnical Officer w.e.f. 1.1.2014.

● Shri Sunil Kumar Sharma, Sr. TechnicalAssistant promoted to the post ofTechnical Officer w.e.f. 1.1.2014.

● Shri Suresh Chand, Sr. TechnicalAssistant promoted to the post ofTechnical Officer w.e.f. 1.1.2015.

NEW JOINING

● Dr. (Mrs.) Megha Pande joined theInstitute as Scientist (AnimalReproduction & Gynecology) on5.4.2014.

● Dr. Yogesh Kumar Soni joined the Instituteas Scientist (Animal Reproduction &Gynecology) on 7.4.2014.

● Shri Dharmendra Singh Verma joined theInstitute as Assistant Finance & AccountsOfficer on 7.4.2014.

● Dr. Ajay Veer Singh Sirohi joined theInstitute as Senior Scientist (LivestockProduction & Management) on29.4.2014.

● Dr. Ravinder Kumar joined the Institute asSenior Scientist (Animal Genetics &Breeding) on 1.5.2014.

● Dr. T.V. Raja joined the Institute as SeniorScientist (Animal Genetics & Breeding)on 7.5.2014.

● Dr. Neeraj Srivastava joined the Instituteas Senior Scientist (Animal Reproduction& Gynecology) on 19.5.2014.

● Dr. Siddhartha Saha joined the Instituteas Senior Scientist (Animal Physiology)on 20.5.2014.

● Dr. Birham Prakash joined the Institute asDirector on 16.8.2014.

● Dr. Naimi Chand joined the Institute asSenior Scientist (Veterinary Medicine) on26.9.2014.

TRANSFER

● Dr. Arun Kumar, Principal Scientist(AG&B) relieved on 17.4.2014 to join thepost of Head, Division of Animal Genetics& Breeding at CSWRI, Avikanagar.

● Dr. D.K. Mandal, Senior Scientist (LPM)relieved on 17.1.2015 on transfer toKalyani, NDRI.

OBITUARY

● Dr. Lakshman Singh Arya, Chief TechnicalOfficer of this Institute expired on10.03.2015 after a prolonged ailment. Thestaff of the Institute observed condolencesfor his untimely demise and prayed Godto rest his soul in peace. The staff of theInstitute also expressed sympathy to hisbereaved family members to bear theirreparable loss.

99



STANDING INSTITUTIONAL COMMITTEES

Purchase Advisory Committee

1. Dr.Rajendra Prasad, Principal Scientist Chairman2. Dr. AK Das, Principal Scientist Member3. Shri DS Verma, AF&AO Member4. Shri Rishi Ram, AO Member Secretary

Local Purchase Committee

1. Dr. AK Das, Principal Scientist Chairman2. Dr. Pramod Singh, Principal Scientist Member3. Shri DS Verma, AF&AO Member4. Indenting Officer Member5. Shri Rishi Ram, AO Member Secretary

Works Committee

1. Dr. Sushil Kumar, Principal Scientist Chairman2. Dr. Mahesh Kumar, Incharge, Estate Member3. Shri CP Singh, CTO Member4. Shri DS Verma, AF&AO Member5. Shri AK Sharma, Asstt. Adm.Officer Member Secretary

Tender Opening, Administrative and Financial Evaluation Committee

1. Dr. Shrikant Tyagi, Principal Scientist Chairman2. Dr. AK Das, Principal Scientist Member3. Shri Rishi Ram, AO Member4. Shri DS Verma, AF&AO Member5. Shri AK Sharma, AAO Member Secretary

Technical Tender Evaluation Committee

1. Dr. Shrikant Tyagi, Principal Scientist Chairman2. Dr. Pramod Singh, Principal Scientist Member3. Dr. DK Mandal, Senior Scientist (upto 17/1/2015) Member4. Dr. Neeraj Srivastava, Senior Scientist (w.e.f 18/1/2015) Member5. Indenting Officer Member



100

Printing Publication Committee

1. Dr. Shrikant Tyagi, Incharge, PME Chairman2. Dr. AK Das, Principal Scientist Member3. Dr. DK Mandal, Senior Scientist (upto 17/1/2015) Member4. Dr. AVS Sirohi, Senior Scientist (w.e.f. 18/1/2015) Member5. Dr. SK Verma, Senior Scientist Member6. Dr. YK Soni, Scientist Member

Committee for prevention of sexual harassment of women

1. Dr. (Mrs.) Saroj Rai, Scientist Chairperson2. Dr. (Mrs.) Megha Pande, Scientist Member3. Smt. Anita Jain, Private Secretary Member4. Shri Vijay Pandit, Chief Functionary, Member

Green Care Society (NGO)5. Shri Rishi Ram, Admn.Officer Member Secretary

Library Advisory Committee

1. Dr. DK Mandal, Sr. Scientist (upto 17/1/2015) Chairman2. Dr. Rajendra Prasad, Pri. Scientist (w.e.f. 18/1/2015) Chairman3. Dr. (Mrs.) Saroj Rai, Scientist Member4. Dr. Rajib Deb, Scientist Member5. Dr. Rafeeque Rahman Alyethodi, Scientist Member6. Dr. (Mrs.) Megha Pande, Scientist Member7. Shri SK Sharma, Technical Officer Member Secretary

RFD Committee

1. Director, ICAR-CIRC Chairman2. Dr. Rajendra Prasad, Principal Scientist Member3. Dr. Umesh Singh, Principal Scientist Member4. Shri Rishi Ram, Admn. Officer Member

Repair, Maintenance & AMC Committee

1. Dr. Pramod Singh, Principal Scientist Chairman2. Shri DS Verma, AF&AO Member3. Shri Rajiv Verma, STO (for other jobs) Member4. Shri YP Malhotra, TO (for IT related jobs) Member5. Indenting Officer Member6. Shri AK Sharma, AAO Member Secretary

101



Rajyabhasha Committee

1. Director, ICAR-CIRC Chairman2. Dr. SK Verma, Senior Scientist Member3. Dr. Rajib Deb, Scientist Member4. Dr. (Mrs.) Megha Pande Member5. Shri Rishi Ram, AO Rajbhasha Adhikari/

Member Secretary

Bio-Safety Committee

1. Dr. AK Mathur, Principal Scientist Chairman2. Dr. Pramod Singh, Principal Scientist Member3. Dr. (Ms.) Rani Alex, Scientist Member4. Dr. Mahesh Kumar, Principal Scientist Member Secretary

Institute Animal Ethics Committee

1. Dr. AK Mathur, Principal Scientist Chairman2. Dr. Sushil Kumar, Principal Scientist Member3. Dr. Sanjeev Kumar Verma, Sr. Scientist Member4. Dr. LS Arya, Technical Officer (upto 9/3/2015) Member5. Shri Vinod Kumar Member6. A Nominee of the CPCSEA Member7. Dr. Mahesh Kumar, Principal Scientist Member Secretary

Staff Welfare Committee

1. Director, ICAR-CIRC Chairman2. Dr. SK Verma, Senior Scientist Member3. Shri Rishi Ram, AO Member4. Secretary (Staff Side), IJSC Member5. Smt. Pushpa, Personal Assistant Member6. Shri AK Sharma, AAO Member Secretary

Institute Deputation Committee

1. Director, ICAR-CIRC Chairman2. Dr. AK Mathur, Principal Scientist Member3. Dr. Umesh Singh, Principal Scientist Member4. Dr. Pramod Singh, Principal Scientist Member



102

Institute Technology Management Committee

1. Director, ICAR-CIRC Chairman2. Dr. Shrikant Tyagi, Incharge, PME Cell Member3. Dr. Pramod Singh, Principal Scientist Member4. Dr. Puneet Kumar, PS, IVRI, Bareilly Member5. Dr. Sushil Kumar, Principal Scientist Member Secretary

Institute Technology Management Unit

1. Director, ICAR-CIRC Chairman2. Dr. Rajendra Prasad, Principal Scientist Member3. Dr. Shrikant Tyagi, Principal Scientist Member5. Dr. Sushil Kumar, Principal Scientist Member Secy./

Nodal Officer

Data Cell

1. PI, Frieswal Project Officer Incharge2. PI, Indigenous Breeds Project Member3. PI, Field Progeny Testing Project Member4. Incharge, S.F. Lab. Member

PME Cell

1. Dr. Shrikant Tyagi, Principal Scientist Officer Incharge2. Dr. Umesh Singh, Principal Scientist Member3. Dr. DK Mandal, Senior Scientist (upto 17/1/2015) Member4. Dr. TV Raja, Senior Scientist (w.e.f 18/1/2015) Member5. Dr. SK Verma, Senior Scientist Member6. Dr. Rani Alex, Scientist Member7. Dr. YK Soni, Scientist Member

Institute Management Committee for ISO-9001 Implementation

1. Dr. Rajendra Prasad, Principal Scientist Chairman2. Dr. Shrikant Tyagi, Incharge, PME Member3. Dr. Umesh Singh, Principal Scientist Member4. Shri Rishi Ram, Administrative Officer Member

Institute Committee for Extension work

1. Dr. SK Verma, Senior Scientist Chairman2. Dr. RR Alyethodi, Scientist Member3. Dr. YK Soni, Scientist Member

103



NODAL OFFFICERS

1. Dr. Rajendra Prasad, Principal Scientist- Nodal Officer, RFD

2. Dr. Shrikant Tyagi, Principal Scientist- Nodal Officer, Tribal Area Sub-Plan (TSP)

3. Dr. Umesh Singh, Principal Scientist- Nodal Officer for implementation of e-publishingof tender details on the Central Public Procurement Portal

4. Dr. Mahesh Kumar, Principal Scientist- Nodal Officer, Aadhar Based BiometricSystem and Swachh Bharat Abhiyan.

5. Dr. Siddhartha Saha, Sr. Scientist- Nodal Officer, IMS/FMS



104

RESULTS-FRAMEWORK DOCUMENT (RFD)FOR

PROJECT DIRECTORATE ON CATTLE (2013-14)Address: Project Directorate on Cattle, Grass Farm Road

P. Box-17, Meerut Cantt (UP)Website: www.pdcattle.ernet.in

SECTION-1

Vision, Mission, Objectives and Functions

Vision: Improvement of cattle for higherproductivity and profitability.

Mission: Germplasm improvement andtechnology development for realizingenhanced productivity and profitability incattle.

Objectives:

1. To develop a national milch breed of cattle‘Frieswal’ using Holstein Friesian XSahiwal base and to test the crossbredbulls under field conditions

2. Genetic improvement of importantindigenous breeds of cattle throughprogeny testing.

Functions:

Development of a new breed of Cattle‘Frieswal’ to produce 4000 kg milk in amature lactation of 300 days with 4.0 % butterfat. Testing of crossbred bulls by evaluationof sires on the basis of performance ofprogenies under field condition.Conservation and genetic improvement ofsome important indigenous breeds of cattlelike Ongole, Gir, Kankrej and Sahiwal in theirnative tract through associated herd testingprogramme. Conduct of basic and appliedresearch programmes related to breeding,feeding, semen freezing and reproduction ofcattle.

105



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SECTION-4

Description and Definition of Success Indicators and Proposed MeasurementMethodology

Objective 1: The Directorate is undertakinga mega project of National importance incollaboration with 37 Military Farms of thecountry located in various agro climaticzones. The semen of meritorious bulls isbeing collected, frozen and distributed toMilitary Farms for AI and up gradation of theFrieswal herds and to stabilize the Frieswalbreed at stipulated targets. Number of semendoses produced, preserved and distributedto Military Farms and others would be the keyto success of propagation of breed. Themeritorious crossbred bulls are progenytested on large scale in fields located at

various parts of the country. By this approachthe bulls are progeny tested under fieldcondition and milk yield of the adoptedfarmers’ cattle is improved by introduction ofsuperior germplasm. The increase in milkyield of the progenies under field conditionshall be the indicator of genetic improvementof farmers’ cattle.

Objective 2: Indigenous breeds of cattle arebeing conserved and improved in their nativetracts in collaboration with SAUs and NGOsthrough associated herd progeny testingprogram.

SECTION-5

Specific Performance Requirement from other Departments

1. The support from Directorate of Frieswaland Military Farms is required in executionof the technical program of FrieswalProject at various farms. Similarly, theselection of young bull calves at variousMilitary Farms is to be taken upappropriately so that required numbers ofbull calves are received at Bull Rearing

Unit for further rearing and semencollection.

2. The required number of history sheets ofFrieswal cows indicating theirperformance with milk yield and otherparameters must be received fromMilitary Farms for analysis of variousbreeding and performance data.



110

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112

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