Ole OlsenDir. Raw Materials

Carlsberg Research LaboratoryBarley Breeding Unlimited

Th

e 27th N

ord

ic Meetin

g o

n B

rewin

g Tech

no

log

y ― 19 M

ay 2016

Johan Kjeldahl@ Carlsberg: 1876-1900

Emil Chr. Hansen@ Carlsberg: 1879-1909

S.P.L. Sørensen@ Carlsberg: 1900-1939

Three shining stars

Strasbourg and Copenhagen

• 45 Project managers and technicians

• 4 Focus areas

- Packaging

- Liquid

- Equipment and technology

- Implementation and optimization

• Short and medium-term focus

Copenhagen

• 85 Scientists and technicians

• 4 Focus areas

- Raw materials

- Yeast and fermentation

- New ingredients

- Brew.tech./quality

• Medium and long-term focus

Carlsberg Group Commercial

Raw materials

Improved raw mat.

New traits

Freshness

Climate tolerance

Sustainability

Yeast and fermentation

Freshness

Fast fermentation

Robustness

New flavour

Functionalities

Brew.tech. and technology

Sustainability

Cost savings

New brewing processes

NAB

New ingredients

Cereal bases,

Ingredients platform

Health & Wellness,

Functionalities

Carlsberg Research Laboratory

• Beer freshness and quality

• Malting quality

• Trait development

• Barley breeding

• Sustainability

• Climate tolerance

• Yield

Consumer benefits

Cost savings

Raw material activities @ Carlsberg

In-field and greenhouse

Carlsberg's focus

remains traditional

breeding

Breeding runs like a clockwork:

Modern, yet traditional

1

2

3

4

56

7

8

i

1 Ideation

2 Translation

3 Bioinformatics

4 Assays

5 Tests of new lines

6 Greenhouse tests

7 Brewing tests

8 Field tests

- Climate tolerance in general

- Heat and drought stress

- Water use efficiency

- Soil management

- Water risks

Standard analyses,

@ Carlsberg: tests, tests …

Bio-

energy

+10 >80%

US corn

GMO

World

population

+50%

2050

Climate

trends

− 4%

World Bank

?

Barley

prices

Barley only

~1% of world's

cereal production

Pressure on tomorrow's barley

Conventional breeding

Brewing benefits

Consumer benefits

Carlsberg and Heineken teamed up 2001-08

Serious support by

recent EPO decision

on plant patentability

plants can be patented –even if the methodsof producing them are not."

Louise Aagaard and Nadine Eckert-BouletHøiberg A/S, patent attorneys

"

Nailing the thing of aged beer's flavour

Efficacious breeding:

Meet a living barley concept

Generation of mutants

Traditional mutagenesis

Mutant finding by high-througput

biochemical screens

Pipeline potential :

A library of +20,000 barley grains

Most grains

with a unique fingerprint

null–LOX-1 barley

and the lipoxygenase pathway

Two null–LOX-1 mutants,

two different gene mutations

null–LOX-1 characteristics

Wild-type Mutant

± null-LOX malt

» Same taste «

Consumer tests

with fresh beers

0.08 ppb T2N

0.05 ppb T2N

0.04 ppb T2N

± null-LOX malt

» null-LOX better«+ null-LOX– null-LOX

Consumer tests

with 10-months-stored beers

Competitive harvest yield

Top barley and malt specifications

Varieties adapted to local climates

Protected supply, Carlsberg's own

null-LOX barley :

Our kind of summit

Joining the club?

Please contact

C a r l s b e r g

Birgitte.Skadhauge@carlsberg.com

H e i n e k e n

Albert.Doderer@heineken.com

Why null–LOX-2 activity

should worry brewers

null–LOX-1

null–LOX-1

null–LOX-2

Wild-type

null–LOX-2

Can double null-LOX

make it better than null–LOX-1?

Beer of double null-LOX malt :

Promising low T2N potential

Forced development of

free , not bound, T2N off-flavour

- Expect 20-80 ppb DMS in beer

- Taste threshold is ~30 ppb

- Malt spec is [DMSP] <4.5 ppm

How two targets

in the SMM cycle regulate DMS levels

ID of one null-SMM plant,

and there are more

Mutant 8063 :

null-SMM, null-MMT, null-DMS

UPLC-based

amino acid

analysis Splice

site

mutationNot

in

beer

Brewing with null-MMT malt

is a better way to beer with no DMS

Heated work:

null-MMT barley

clears several

DMS hurdles

Added benefit:

DMS is a

climate gas!

And next, already ongoing,

xxxxxxxxxxxxxxxxxxxxxxxxxxxx

New era, shaping things:

Building the future by boundary pushing

• Barley genome sequence

• Molecular technologies

• Reduced breeding time

• Trait discovery

and development

Credits

The International Barley Genome Sequencing Consortium

Nature 491, 711, 2012

Søren

Knudsen

Gustav

Hambræus

Finn

Lok

Birgitte

Skadhauge

DLG

Lene

Bech

Preben

Riis

Klaus

Breddam

DMG

?