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Bio Basic Inc.

NEXT GENERATION SEQUENCING ORDER FORM(DNA SAMPLES)

1. BASIC INFORMATION

NAME OF COMPANY CONTACT PERSON TELEPHONE NUMBER

EMAIL ADDRESS ADDRESS LAB NAME GROUP / P.I. RESEARCH OBJECTIVE

2. EXPERIMENTAL INFORMATION

BIORESEARCH FIELD

GenomeTranscriptome LevelEpigeneticsOther (Please specify below)

Other: EXPERIMENTAL DESIGN De Novo Sequencing Genome Survey

Whole Genome Re-sequencing

Exome Sequencing

Whole Genome Bisulfite Sequencing

ChIP Sequencing

Metagonomic Sequencing GenotypingTarget Region Sequencing MeDIP

SequencingOther:

20 Konrad Cres, Markham Ontario L3R 8T4 CanadaTel: (905) 474 4493, (800) 313 7224 Fax: (905) 474 5794Email: [email protected] Web: www.biobasic.com

A world leader in serving science

Bio Basic Inc.

3. SAMPLE INFORMATION

TYPE OF SAMPLE Genomic DNAPCR ProductsBroken Short FragmentsChIP-Seq DNA SamplesOther (Please Specify Below)

Other: STATE OF SAMPLE Dissolved in Water

Dissolved in TE BufferDissolved in Pure EthanolOther (Please Specify Below)

Other:

4. SAMPLE DETAILS

Sample Name

Species # of Tubes

Concentration (ng/µl)

Volume (µl)

Total Quantity (µg)

Fragment Size

Please send the completed form to: [email protected]

SAMPLE CONDITION: DNA Samples without degradation or RNA contaminationSAMPLE QUANITITY: m≥6µgSAMPLE CONCENTRATION: 50 ng/µlSAMPLE PURITY: OD 260/280 = 1.8 ~ 2.0



Bio Basic Inc.

5. SAMPLE REQUIREMENTS

Library Types Sample Types Quantitative Methods

Quantitative ResultQubit

≤800 bp Insert

Sample With Single Band or Main Apparent Band in

Electrophoresis (genome, plasmid, PCR product)

Qubit m≥6µg c≥30µg

Sample for Meta-genomics Sequencing

Qubit m≥4µg c≥30µgSamples With Smear in

Electrophoresis: Not Fragmented

Qubitm≥3µg c≥30µg

Samples With Smear in Electrophoresis: Fragmented

Qubit m≥4µg c≥30µg2K Insert Genomic DNA Qubit m≥40µg c≥133µg

5-6K Insert Genomic DNA Qubit m≥40µg c≥133µg10K Insert Genomic DNA Qubit m≥60µg c≥133µg

20/40K Insert Genomic DNA Qubit m≥120µg c≥133µg

PCR Free Genomic DNA Qubit m≥20µg c≥30µgPCR Product with Single Band Qubit m≥6µg c≥30µg

ChIP-Seq ChIP-Seq DNA Samples Qubit, Agilent 2100 m≥20µg

MeDIP/Bisulfite Genomic DNA Qubit m≥10µg c≥50µgEXON Agilent, Nimblegen EZ,

Nimblegen Array (pooling)

Genomic DNAQubit

m≥6µg c≥50µg

EXON Nimblegen Array (one sample for

Hybridization)Genomic DNA Qubi m≥30µg c≥50µg

Genotyping Genomic DNA Qubit, m≥2µg c≥50µg



Bio Basic Inc.

6. Terms and Conditions

TermsBio Basic Inc. will send electronically DNA sequencing results to customers. All results will be kept in Bio Basic Inc.’s data base for 6 months. During this period, customer may request sequencing results. After 6 months, DNA sequencing results are automatically destroyed without further notification.

DNA sequencing tests are not considered confirmed without the customer providing a P.O. number or credit card number. Bio Basic Inc. accepts credit card (Visa or MasterCard), cheque, or wire for payment.

CancellationsBuyer may not cancel any order or return any samples without Bio Basic Inc.’s consent. Cancellation and return charges may be charged. Buyer must contact Bio Basic Inc. to obtain a return material authorization number.

WarrantyBio Basic Inc. does not guarantee any downstream application(s) or usage of the DNA sequencing results. Any claims or dispute must be submitted within 2 weeks of the results being sent. At the time of shipment, Bio Basic Inc. keeps a database for unexpected incidents and/or disputes. Bio Basic Inc. reserves the right to refuse handling disputes after a period of 3 months.

PricesAll prices are subject to correction of errors; Bio Basic Inc. reserves the right to adjust prices on orders during production due to changes in cost of materials, transportation or wages. Any tax, customs, surcharge or duty, howsoever denominated, imposed upon the sale, importation, delivery or use of products shall be the responsibility of the Buyer.



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