Online FCM explains more!Stefan Zimmermann

SIGRIST-PHOTOMETER AG

Switzerland

What is online FCM?

Content here

Online Sampling Device

• Mobile 14.5 kg

• Variable interval

• 30 min. to 6 hours

• 1’000 measurements

Measurement

1. Pump sample through flow cell

2. Measure simultaneously with 3 detectors

1. Channel 1 – Side Scattering (SSC)

2. Channel 2 – Fluorescence 1 (FL1)

3. Channel 3 – Fluorescence 2 (FL2)

Raw Signals

• Every cell generates signal peaks on the 3 channels SSC, FL1 and FL2

Example of gating

• Gate := Region of dot plot with cells

• Dotplot shows1. Noise

2. LNA-cells

3. HNA-cells

4. Debris

Communication

• USB

• Ethernet

• 2 x mA

• 4 x digital output

Result Evian fresh

Result Evian after 1 day

Biofilm in the tubing

Biofilm detection

Detected through%HNAP

Pump trial: TCC, HNAC, pumprate, particle counter

FL1/FL2 fingerprint

Desinfection a

Desinfection b (after 1h)

Desinfection c (after 6h)

Rain / TCC correlation

TCC, HNA at Bern airport

Bern airport

Peaks 4 to 8 times higher

• mp4 file: «Koniz-04-to-11_09.mp4»

Nymphal form gets adults

Presentation today

• In collaboration with bNovate: Startup company, Lausanne

• Some slides originated from Prof. emeriti Thomas Egli (eawag) andJonas Amstutz, SIGRIST-PHOTOMETER AG

• Data and photos are taken from the test locations Basel, Ennetbürgen, Köniz and Zürich in Switzerland

Thank You for your attention