onsite bio medical waste treament
TRANSCRIPT
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Biomedical waste management
Farid khan
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Biomedical wastes are treated onsiteutilizing autoclaves
Autoclaving provides an economical process of killingpathogenic microorganisms through saturation withmoist heat (steam) under pressure
This process makes the microorganisms nonviable bydestroying (denaturing) essential proteins andstructures
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Waste Handling Continued
Bags should be left to cool forseveral minutes beforeremoving from autoclave
Once cool, securely closebiohazard bag and place intoopaque black bag which haslarge white trash bin assecondary containment
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Autoclave Use Log
Record
date wasteis treated
Give a
description
of waste
treated
Record
weight of
material
in pounds
Always record
conditions for the
treatment of Waste
Record duration
waste is treated
and the cycle
number
Recordname of
the person
running
the cycle
Record
initials of
person
running
cycle
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Quality Control
Methods for Quality Control: Autoclave temperature tape (only tells desired
temperature reached not time or pressure)
Parameter monitoring (pressure, time and temperature)
Record every load on autoclave use log Perform Efficacy monitoring
0 - 100 lbs/month - monthly
100 - 200 lbs/month - biweekly
>200 lbs/month - weekly
Routine parameter monitoring maybe substituted for
biological monitoring for autoclaves which have a
continuous readout and record of operating parameters
(example: autoclave with receipt tape style printout)
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Parameter Monitoring
Parameter monitoring is necessaryto ensure efficacy of autoclave
Parameter can be performed byusing Verify integrator strips
Autoclaves that use pinwheel plotsrequire biological monitoring withVerify biological monitoring vialscontaining live GeoBacillus
Stearothermophilus
Verify Self Contained Biological
Indications
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Autoclave Parameter Log
Staple the Verify
indicatorstrip from cyclehere.
Print the name
and initials of
person running
the cycle.
Record
the date
Indicator
Strip was
run
Please
record the
temperature,
pressure,
duration, and
the cyclenumber
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Autoclave Rooms are BSL-2 Rooms
It is important to rememberthat all autoclave rooms areBSL-2 (biosafety level 2) rooms
Indicates a biohazard is
present such as Hepatitis Bvirus, HIV, the salmonellae,and Toxoplasma spp. Alsoincludes human-derived blood,body fluids, tissues, or primaryhuman cell lines where thepresence of an infectious agentmay be unknown
Indicates that NO FOOD /
DRINKS or cosmeticsareallowed in the room
Typical Door Posting
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Personal Protective Equipment
When selecting PPE consider the highest level hazard present,the source of the hazard, and the potential forsimultaneous exposures.
Face protection Goggles or safety glasses with side shields
Clothing Lab coats, scrub suits, gowns (long pants only, no open toed
shoes) Clothing should be replaced immediately when contaminated
Gloves Verify gloves are compatible with your specific application,
process and materials before using Gloves should be replaced immediately if torn Gloves should not be worn outside the lab area Always wear heat resistant gloves and keep your face away
from the door when loading and unloading the autoclave
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Employee Safety Practices
Avoid compressing bags which may create aerosols
Biological waste should be stored in rigid leak proofcontainers such as biohazard boxes, bins, or secondarycontainment pans
Waste should be stored inside the autoclave room andnot in the hallways
Biological waste containing hazardous chemicals
(flammables, chemotherapeutic agents) or Sharpsshould not be autoclaved. Call EH&S at 713-500-5837 for collection of these items
Biological Waste should not be left for someone elseto autoclave
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Good Employee Work Practices
Be a conscientious worker Keep work areas clean and free of hazards
Use Standard Precautions
Treat all human blood and body fluids as ifknown to be infectious
No eating, drinking, or applying cosmetics in the workarea
Frequent hand washing
Decontaminate work surfaces
decontaminate surfaces daily
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Common Biomedical Waste AutoclaveDeficiencies
EH&S performs semiannual surveys ofautoclaves utilized for biomedical wastetreatment
Common deficiencies include: Food or drink consumed or stored in
autoclave area Autoclave log incomplete Sterility monitoring not performed Training records incomplete Minors in the work place
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In Summary
Autoclave Use Guidelines to follow:
3. Place sterilizing indicator tape on the autoclave bag. Make sure bags
designated for autoclaving are used
2. Place waste inside autoclave. Use secondary containment (pan) under the bag.
If waste does not contain any moisture, put cup of water inside bag for
steam generation
3. Autoclave according to manufacturers instructions for a minimum 30 minutes,
at a minimum operating temperature and pressure of 250 deg. F and 15 psig
4. When cycle is finished, inspect sterilizing tape, and visually check bags to
ensure autoclave was working correctly5. Place waste in black opaque bags. Seal bag with tape or tie wrap
6. Place waste in trash cans labeled Autoclaved Waste Only Log activities in the autoclave usage log Perform routine efficacy monitoring and record results in logbook