optimization of the abencor system to extract olive oil from irrigated orchards
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Research Article
Optimization of the Abencor system to extract olive oil fromirrigated orchards
Eric Ben-David1,2, Zohar Kerem2, Isaac Zipori1, Sebastian Weissbein1, Loai Basheer2,
Amnon Bustan1 and Arnon Dag1
1 Gilat Research Center, Agricultural Research Organization, M. P. Negev, Israel2 Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture,
Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Studying the composition of olive oil requires cold-press olive oil extraction. One of the most
common laboratorial mills is the Abencor system. However, its operation protocol was formulated
decades ago for Spanish olive varieties from traditionally rain-fed orchards. We modified this protocol
for use with ‘‘Barnea’’ and ‘‘Picual’’ olives from irrigated orchards that are characterized by high
water content. Independent effects of malaxation time, temperature, water addition and talc
addition on extraction efficiency, and major quality indices of virgin olive oil were studied.
Overall, addition of talc to the fruit paste was the most significant treatment in terms of yield and
quality of the oil although its effect was cultivar dependent. Improved oil yield was particularly significant
for ‘‘Picual.’’ Extended malaxation time was also effective in improving oil extractability. Addition of
talc generally improved oil-quality parameters, while water addition had the opposite effect. Yet,
quality parameters remained within the extra virgin level. Temperature increments reduced oil
quality. The need to adapt a modified protocol for use with fruits from irrigated orchards that will
facilitate critical comparison of results obtained from different agronomic theses and different
laboratories is highlighted. It is recommended that each laboratory develops an appropriate protocol
for the operation of the Abencor system in accordance to the characteristics of the olive fruit they are
working with.
Practical applications: Abencor system serves as the major laboratorial mill world-wide. Those
mills allow the researchers to characterize olive oil in accordance to the treatments received by
the trees. This cannot be done in commercial mills. The system operation protocol was established
decades ago for fruits from rain-fed orchards. In the past decade there was a rapid increase in the use of
irrigation in olive orchards and therefore it is crucial to optimize the operation protocol for fruit with
relatively high water content. In the current work we have evaluated the influence of a series of
technological parameters (i.e., talc and water addition, malaxation time, and temperature) on the
extraction efficiency and quality indices of olive oil. This allowed us to present a modified
protocol for the Abencor system operation suitable for olive fruit of irrigated orchards that will facilitate
a reliable representation of the influence of different treatments on the yield and characteristics of the
olive oil.
Keywords: Abencor / Irrigation / Malaxation / Protocol / Virgin olive oil
Received: February 17, 2010 / Revised: June 1, 2010 / Accepted: June 25, 2010
DOI: 10.1002/ejlt.201000056
1 Introduction
Olive (Olea europaea L.) oil is a basic constituent of the
Mediterranean diet [1]. Its consumption has significantly
increased in recent years as a result of its nutritional value
Correspondence: Dr. Arnon Dag, Gilat Research Center, Agricultural
Research Organization, M. P. Negev, 85280 Israel
E-mail: [email protected]
Fax: 972-8-9926485
Abbreviation: FA, free acidity
1158 Eur. J. Lipid Sci. Technol. 2010, 112, 1158–1165
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and recognized benefits for human health [2–4]. To meet the
increased demand, irrigation has been introduced to
traditionally rain-fed olive oil orchards and the plantation
of irrigated high-density orchards has intensified [5–6].
These trends have led to a substantial increase in the pro-
duction of olive oil [7–9].
Several studies have demonstrated the effects of irrigation
on oil quality [6–15]. Other factors, genetic, and agronomic,
such as fertilization, cultivar, cultivation technique, date and
method of harvest, transportation and storage of fruits, and
method of oil extraction, have all been reported to influence
the composition and organoleptic characteristics of virgin
olive oil [16–18].
Virgin olive oil production, unlike that of other vegetable
oils, is a mechanical process that consists of crushing, malax-
ation, and oil separation [18, 19]. Retaining the olive flavor
and aroma attributes throughout this process is of prime
importance with respect to the oil’s organoleptic, nutritional,
and oxidative-resistance properties [20]. Unfortunately, the
study of the relations between cultivation practices, yield,
and quality of oil in industrial olive mills is limited by the
continuous mode of operation, the requirement of large
amounts of fruit due to the size of the machinery, and the
lack of homogeneity among those fruits [18]. Consequently,
the development of laboratory-scale mills such as the
Abencor system have facilitated research into the effects of
various agronomic practices on quality indices of olive oil [14,
19–25] and yield [26–28] of olive oil. The Abencor system is
advantageous due to its batchmode of operation, the reduced
amount of fruit needed for statistically reliable results and its
convenient control of the operation parameters [21].
However, the recommended operation conditions of the
Abencor system were formulated several decades ago for
use with Spanish varieties of olives, cultivated in traditionally
rain-fed orchards [21]. The flesh of these fruit contains
considerably less water (�40%) than that of varieties grown
under intensively irrigated conditions in modern orchards
(�60% water). In addition to other parameters, the
water content of the raw material hence the olive paste
is a dominant factor determining the efficiency of the
extraction process and other oil quality parameters. There
is therefore a clear need to define major principles
for a flexible though standard Abencor protocol thus
providing necessary tools for the evaluation of oil yield
and quality from diverse origins (irrigated vs. rain-fed
groves, cultivars, etc.) on the laboratory scale. Noteworthy,
any attempts to relate lab- to industrial-scale of olive oil
yield and quality should be done with caution due to differ-
ences between the systems in the oxygen control, heat
exchange as well as in other parameters [29, 30]. Here
we demonstrate how the adjustment of the Abencor
laboratory scale system to the use with olive fruit of two
cultivars (‘‘Barnea’’ and ‘‘Picual’’) produced in commercial
irrigated orchards facilitates the oil production efficiency
while retaining optimal oil quality.
2 Materials and methods
2.1 Oil extraction
Olive fruits were obtained from a commercially irrigated
orchard in the southern part of Israel in the Negev Desert.
The average annual rainfall in that area is around 100 mm.
Plants were distanced 3 m � 7 m. Due to the high evapo-
transpiration and low rainfall, total amount of water supplied
to the trees is ca. 1000 mm/year.
Fruits from two cultivars, ‘‘Barnea’’ and ‘‘Picual,’’ were
mechanically harvested using trunk-shakers, on January 23,
2007 and January 28, 2008, respectively. The degree of ripe-
ness, estimated by fruit color according to Uceda and Frıas
[31], was within the acceptable range for harvest in Israel [3.79
in ‘‘Barnea’’ (2007) and 3.61 in ‘‘Picual’’ (2008)]. Average
water content in the crude pastes was 59 and 68% in ‘‘Barnea’’
and ‘‘Picual,’’ respectively. Percentage of stone in the pulp was
15.4 and 12.4% ‘‘Barnea’’ and ‘‘Picual,’’ respectively.
For each treatment, four 1.0-kg replicates were randomly
sampled from the harvested fruits. Oil was extracted using an
Abencor system small-quantity mill, simulating commercial
oil-extraction systems (MC2 Ingenierıa Sistemas, Seville,
Spain). The olives were crushed with an Abencor hammer
mill equipped with a 4-mm sieve and 700 g paste were proc-
essed using the Abencor system’s malaxer and centrifuge.
The recommended conditions specified by Abencor’s
operation protocol were used as the baseline [21]. Three treat-
ments: malaxation temperature (25, 30, 35, and 40 8C),
malaxation time (30, 60, 90, and 120 min), and amount of
water added to the paste (0, 100, 150, 225, and 300 cm3) were
evaluated with and without talc addition. In ‘‘Barnea’’, the
effect of malaxation temperature was tested only with talc
addition. Fixed conditions were applied according to the
Mc2 Ingenieria Sistemas’s suggested protocol: temperature
of the paste was fixed at 35 8C when studying malaxation
time and water addition; malaxation time was fixed at
30 min when studying temperature and water addition, and
water addition was fixed at 300 cm3 water per 700 g paste in
the malaxation time and temperature treatments. The talc
treatment consisted of adding 40 g of talc (Talcoliva,
Bonar Leon, Spain) to the paste. Water was added 20 min
after the start of malaxation in accordance with Martinez et al.
[21]. Extracted oil percentage was determined according to
the formula [21]:
Oil percentage ð%Þ ¼ cm3 of obtained oil � 0:915
weight of the paste
� �� 100
2.2 Oil analysis
Determination of free acidity (FA) and peroxide values was
carried out following the analytical methods described in ISO
(International Organization for Standardization) 660 and
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3960, respectively. FA, given as percentage of oleic acid, was
determined by titration of a solution of oil in ethanol–diethyl
ether (1:1 v/v) with an ethanolic solution of 0.01 N KOH.
Phenolphthalein solution in ethanol (1% w/v) was used as an
indicator. Peroxide value, expressed as milliequivalents of
active oxygen per kilogram oil (meq O2 kg), was determined
by sodium thiosulfate titration (0.01 N) of free iodine from a
mixture of oil and iso-octane:acetic acid (2:3 v/v) left to react
with potassium iodide solution in the dark for 1 min. Starch
solution (1% w/v) was used as an indicator.
Phenolic compounds were isolated from a solution of oil
in hexane by double-extraction with methanol–water (60:40
v/v). Total phenols, expressed as tyrosol equivalents (mg/kg),
were determined with a UV–Visible spectrophotometer
(Beckman Coulter, Inc. Fullerton, CA) at 735 nm using
Folin–Ciocalteu reagent according to Gutfinger [32].
2.3 Statistical analysis
Data were analyzed using JMP 5.0 software (SAS institute,
Inc., Cary, NC). Effect of the various treatments on oil yield
and quality were examined by ANOVA, and whenever the F
statistics was significant, differences between treatments were
determined using Tukey–Kramer honestly significant differ-
ence test (at p � 0.05). Due to the low extraction efficiency of
‘‘Picual’’ samples without talc addition, replicates were
merged and the statistical significance of their quality indices
was not evaluated.
3 Results and discussion
3.1 Duration of malaxation
The duration of malaxation has a major influence on the
yield of extracted oil as well as on the oil’s quality properties
[25, 33–36]. Malaxation time has been shown to relate posi-
tively to the total content of volatiles in virgin olive oil, and
negatively to total phenol concentration [18, 36]. In the
current study, the effect of malaxation time, with and without
addition of talc, on the yield and properties of olive oil was
evaluated in ‘‘Barnea’’ and ‘‘Picual’’ oils (Tables 1 and 2).
Results of factorial ANOVA indicated that the independent
effect of malaxation time on the percentage of oil extracted
was highly significant in ‘‘Barnea’’ and ‘‘Picual’’ samples,
while the added talc only had a substantial effect on
‘‘Picual’’ (Table 1). Increased malaxation time, without talc
addition, resulted in a significant increase in the percentage of
extracted oil, concomitant with a substantial effect on its
quality parameters (Table 2). Increased levels of extracted
oil concurrent with increased malaxation time have also been
noted by Di Giovacchino et al. [34]. Increasing the malax-
ation time of ‘‘Barnea’’ samples from 30 to 90 min, without
added talc, almost doubled the oil yield: 92 g/kg of pasteas
compared to 174 g/kg of paste. However, in ‘‘Barnea’’
samples with added talc, the samemalaxation time increment
corresponded with only a modest increase in the yield of
extracted oil (from 128 to 159 g/kg of paste). On the contrary,
the addition of talc had a remarkable positive effect on the
yield of oil extracted from ‘‘Picual’’ fruits, 135 to 179 g/kg of
paste compared with 19 to 84 g/kg of paste, where no talc was
included. The combination of talc addition with the longest
malaxation time, 120 min, led to the highest yield (179 g/kg
of paste). The independent effects of malaxation time and
talc addition on the yield of oil from ‘‘Picual’’ were highly
significant (p<0.0001; Table 1).
The operation of the original Abencor protocol with high
water content fruit (68% in fruit flesh) from irrigated
‘‘Picual’’ orchards yielded extremely low oil levels that are
also sub-representative in terms of their quality indices, and
are potentially far from economical in a commercial mill. Our
Table 1. Combined effect of Abencor operating conditions (temperature, malaxation time, and amount of water added), with and without talc
addition, on olive-oil extraction efficiency and quality parameters
Oil percentage (%) ‘‘Barnea’’
‘‘Picual’’ ‘‘Barnea’’ Acidity (%)
Peroxide value
(meq O2 kg/oil)
Polyphenol
concentration (mg/kg)
Talc ��� NS ��� ��� ���
Malaxation time ��� ��� � �� ��
Talc � malaxation time NS �� �� �� �
Talc ��� ��� ��� ��� ��
Amount of water ��� �� � � ���
Talc � amount of water ��� � �� �� NS
Temp ���
Talc ��
Talc � Temp ��
One way ANOVA and post-hoc Tukey–Kramer multiple comparison test were conducted individually for different parameters. NS ¼ not
significant;� p � 0.05; �� p � 0.01; ��� p � 0.001.
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results suggest that addition of talc and increasing the
duration of malaxation are indispensible for that type of fruit.
In commercial mills, the addition of talc to the centrifugal
decanter has been shown to improve its efficiency and to
increase the oil yield [34, 37, 38]. Conversely, in fruits with
lower water content such as ‘‘Barnea’’ (59%), practical
extraction efficiency may be achieved without the addition
of talc. Still, the extension of the malaxation time significantly
improved the efficiency of oil extraction as compared with the
original Abencor protocol.
Water content in the fruit flesh is a significant parameter
of difference between the two cultivars hence the
adjustments required for the Abencor protocol. Talc, func-
tioning as a water absorber, modifies the ‘‘difficult paste’’ of
high water content fruit thus smoothing the extraction
process.
Factorial ANOVA revealed that the independent effects
of malaxation time, talc addition, and their interaction on the
oil composition of the ‘‘Barnea’’ samples were statistically
significant (Table 1). In ‘‘Picual’’ samples, the combined
effect of a long malaxation time (120 min) and talc addition
produced the highest FA values (Table 2). Surprisingly,
reduced FA levels in ‘‘Barnea’’ samples were associated with
a malaxation time increment from 30 to 90 min in samples
without talc (Table 2). Addition of talc led to lower peroxide
values in ‘‘Barnea’’ and ‘‘Picual’’ oils relative to the no-talc
treatments, particularly at long malaxation time (Table 2).
These results may again demonstrate the uniqueness of
results achieved using olive fruits from irrigated orchards,
when compared to other studies that did not find any sig-
nificant association between malaxation times ranging from
15 to 90 min or talc addition and oil quality parameters [34,
38]. The effect of talc addition on oil properties was highly
significant, while malaxation time induced only mild changes
in the oil’s composition (Table 1). Malaxation time corre-
lated negatively to polyphenol content in ‘‘Picual’’ oils, but
surprisingly, showed an opposite trend in ‘‘Barnea’’ oils,
irrespective of the talc treatment (Table 2). Increased poly-
phenol content in ‘‘Barnea’’ oils was associated with a 30–
60 min malaxation time increment while in ‘‘Picual’’ oils,
polyphenol content decreased when malaxation time was
raised above 60 min. Factorial ANOVA revealed significant
effects of malaxation time, talc addition, and their interaction
on ‘‘Barnea’’ oil polyphenol content (Table 1). Increased
malaxation time has been reported to decrease polyphenol
content by up to 20% in commercial mills [36, 25] and by up
to 60% in a small laboratory apparatus, as compared with
commercial mills [36]. A similar trend was found in the
present study for ‘‘Picual’’ but not for ‘‘Barnea.’’ Talc
addition does not significantly influence polyphenol content
according to Cert et al. [38]. Our results suggest, however,
that polyphenol levels in oils from the two tested cultivars are
significantly, albeit inconsistently, influenced by the addition
of talc. Nevertheless in general, all of the oils produced in the
present study were rated extra virgin in terms of peroxide and
FA values. All the differences even when statistically signifi-
cant remained within the extra virgin quality level and there-
fore, had only minor practical relevance.
3.2 Water addition
The addition of water to the paste during malaxation to
improve oil extractability was suggested several decades
ago by Martinez et al. [21]. Their recommended protocol
specified the addition of two portions of boiling water, the
first (300 cm3) after 20 min of malaxation, and the second
Table 2. Influence of malaxation time and talc addition on percentage of extracted oil, oil acidity, oil peroxide value, and oil polyphenol
concentrationa)
Treatment
Malaxation
time (min)
Oil percentage
(%)
FA (expressed
as % oleic acid)
Peroxide value
(meq O2 kg/oil)
Polyphenols
content (mg/kg)
No talc (‘‘Barnea’’) 30 9.20b 0.52a 13.94b 36c
60 15.11a 0.43ab 13.66b 60bc
90 17.42a 0.36b 20.01a 107ab
Talc (‘‘Barnea’’) 30 12.81ab 0.32b 12.55bc 83bc
60 14.62a 0.32b 10.35c 152a
90 15.93a 0.35b 11.17bc 153a
No talc (‘‘Picual’’) 30 1.87A 0.29 4.76 239
60 3.52AB 0.30 3.00 238
90 3.25AB 0.38 5.21 199
120 8.38B 0.51 7.19 121
Talc (‘‘Picual’’) 30 13.47C 0.38A 5.21A 199A
60 15.28C 0.34A 3.79A 224A
90 15.44CD 0.39A 3.28A 90B
120 17.91D 0.69B 4.87A 65B
a) Values are means of four replicates. Different letters indicate significant differences between treatments, p � 0.05.
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(100 cm3) after the first centrifugation. They further
suggested the addition of talc to the paste to improve oil
extractability in the system [21]. However, the addition of
water to olive paste, typically associated with a ‘‘three-phase’’
decanter, has been reported to be negatively related to total
phenol and o-diphenol contents compared with the ‘‘two-
phase’’ decanter, which uses less water [39]. Our results, in
agreement with previous studies, demonstrated a significant
increase in oil extractability following the addition of talc;
conversely, we found reduced oil extractability associated
with water addition treatments of �150 cm3 and no talc
addition in both cultivars (Table 3). Factorial ANOVA tests
revealed high significance of the effects of talc and water
addition and their interaction on oil extractability in both
cultivars (Table 1). In light of the negative effect of water
addition during malaxation on oil extractability, it is
suggested that this practice be avoided for fruits from irri-
gated orchards as they already contain considerable water
levels.
Water addition had a significant effect on the quality
indices of the ‘‘Barnea’’ oils (Table 3). FA was significantly
reduced in treatments that included talc addition (0.29–
0.39%) as compared to those without talc (0.46–0.61%).
Interestingly, increasing the amounts of added water in pastes
with added talc was associated with a slight increase in acidity
values for both cultivars. Peroxide values for ‘‘Picual’’ were
approximately one-third those of ‘‘Barnea’’ and did not
respond to the amount of added water (Table 3). In
‘‘Barnea’’, however, peroxide values were significantly higher
for treatments that included a higher amount of water (150–
225 cm3) and talc addition and lowest for the oil treated with
150 cm3 of water without talc addition. Factorial ANOVA
results suggested that the effects of talc and water additions
and their interaction significantly influence the acidity and
peroxide values of the ‘‘Barnea’’ oils (Table 1). Increasing the
levels of added water led to a significant decrease in poly-
phenol content in ‘‘Barnea’’ oils. Conversely, the polyphenol
content in ‘‘Picual’’ oils increased significantly when the
water level was increased to 300 cm3 (Table 3). Addition
of 300 cm3, according to Abencor’s protocol, to ‘‘Barnea’’
oils without talc led to very low levels of polyphenols. The
highest polyphenol levels were found in ‘‘Barnea’’ oils with
added talc and the lowest amount of added water (100 cm3),
in agreement with previous reports [34, 36]. It has been
suggested that phenols of a hydrophilic nature decrease as
a function of the amount of water added [40]. Again, most of
the changes in quality parameter due to the addition of water
were within the range of extra virgin olive oil level. Practically
therefore, where no significant advantage occurs, water
addition to the paste of irrigated olive fruit should be avoided.
3.3 Temperature
Olive paste kneading can be improved by heating, which
reduces the oil’s viscosity, helping the oil drops coalesce
and leading to more readily extractable oil from the vegetable
tissue [25]. Unfortunately, increasing the malaxation
temperature degrades overall oil quality as the more volatile
Table 3. Influence of water and talc addition on percentage of extracted oil, oil acidity, oil peroxide value, and oil polyphenol concentrationa)
Treatment
Water
addition (cm3)
Oil percentage
(%)
Acidity
(% oleic acid)
Peroxide value
(meq O2 kg/oil)
Polyphenols
content (mg/kg)
No talc (‘‘Barnea’’) 100 13.80b 0.61a 14.21abc 216a
150 12.65b 0.46b 12.97c 124bc
225 9.36c 0.50ab 13.24bc 105c
300y 9.20 0.52 13.94 36
Talc (‘‘Barnea’’) 0y 18.40 0.32 13.25 276
100 17.09a 0.30c 13.25bc 237a
150 16.10a 0.29c 15.73ab 178ab
225 16.26a 0.39bc 16.56a 144bc
No talc (‘‘Picual’’) 0 9.69A 0.21 2.94 125
100 9.04A 0.23 3.57 159
150 8.71A 0.24 3.28 260
225 2.96B 0.20 4.00 292
300 1.87B 0.17 4.08 136
Talc (‘‘Picual’’) 0 13.96C 0.21A 5.66A 129A
100 12.81C 0.15A 4.47A 115A
150 14.29C 0.26A 4.98A 129A
225 14.46C 0.32A 4.81A 105A
300 13.47C 0.38A 5.21A 199B
a) Values are means of four replicates. Different letters indicate significant differences between treatments, p � 0.05.
y Statistical data analysis was not performed.
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aromas are lost, the rate of oil oxidation is increased and the
polyphenol content decreases [41–43]. Talc addition can be
used to improve the efficiency of the oil extraction by enabling
a reduction in both mixing time and temperature [38]. In the
present study, factorial ANOVA revealed significant effects of
temperature and talc addition and their interaction on oil
yield of ‘‘Picual’’ (Table 1). Increasing malaxation tempera-
ture from 25 to 40 8C did not improve oil yield in samples
without talc, irrespective of cultivar type. However, in
‘‘Picual’’ samples with added talc, the temperature increment
produced a significant effect, increasing the oil yield by 44%
from 106.8 of paste to 154.4 g/kg paste (Table 4). This effect
was already reflected by the 28% rise in oil yield when
temperature was increased from 20 to 40 8C at a fixed malax-
ation time of 60 min [25].
Changes in malaxation temperature had a significant
effect on the quality-related analytical indices considered
(Table 4). Initial assessment of the effect of malaxation
temperature on ‘‘Barnea’’ oil samples without added talc
demonstrated a decrease in polyphenol level when the
temperature was raised from 25 to 40 8C (Table 4). The
influence ofmalaxation temperature on oil quality was further
evaluated in ‘‘Picual’’ oil samples with talc addition
(Table 4). Likewise, raising the temperature from 25 to
40 8C reduced the level of polyphenols in ‘‘Picual’’ oils while
concomitantly inducing an increase in FA and peroxide val-
ues (Table 4).
4 Conclusions
The major difference between fruit of traditional rain-fed
and irrigated oil olive trees is in the water content of the fruit
flesh, about 40% versus 60%, respectively. In the original
Abencor method, which was developed for rain-fed olives,
water addition was required to improve oil extraction [21],
while for irrigated olives, the removal of excess water from
the paste is essential to achieve considerable efficiency of
the extraction process, much closer to levels obtained by
commercial mills. In the present study, we modified the
traditional, laboratory-scale Abencor method and attempted
to adjust it to fruit of irrigated olive orchards. The addition of
talc in order to absorb excess water, smooth the malaxation
process and consequently improve the oil yield is the main
technical adjustment required. The addition of water
should be avoided. The malaxation time should be
extended according to the water characteristics of the raw
material. These modifications increase the oil yield without
significant practical changes in the oil quality parameters.
Increased temperature may contribute to the efficiency of
the extraction process, yet it might affect quality parameters
such as the polyphenols content and therefore should
not exceed 35 8C. Different cultivars may require ad hoc
fine-tuning of the technological parameters. Differences in
the varieties response may relate to differences in their
respective average water content, percentage of stone in
the fruit, and flesh consistency. The work with an adjusted
cultivar dependent protocol suitable for fruit of irrigated
orchards will facilitate reliable representation of the influence
of different treatments on the yield and characteristics of the
olive oil.
The authors have declared no conflict of interest.
References
[1] Trichopoulou, A., Critselis, E., Mediterranean diet and lon-gevity. Eur. J. Cancer Prev. 2004, 13, 453–456.
[2] Keys, A., Mediterranean diet and public health: Personalreflections. Am. J. Clin. Nutr. 1995, 61, 1321S–1323S.
Table 4. Influence ofmalaxation temperature and talc addition on percentage of extracted oil, oil acidity, oil peroxide value, and oil polyphenol
concentrationa)
Treatment
Temperature
(8C)
Oil percentage
(%)
Acidity
(% oleic acid)
Peroxide value
(meq O2 kg/oil)
Polyphenols
content (mg/kg)
No talc (‘‘Barnea’’) 25 10.51a 0.45ab 12.97a 75a
30 9.53a 0.37a 13.94a 30b
35 9.20a 0.48b 13.94a 36b
40 9.20a 0.36a 14.63a 18b
No talc (‘‘Picual’’) 25 1.54A 0.59 8.15 109
30 2.96A 0.22 3.17 107
35 1.87A 0.17 4.08 136
40 1.48A
Talc (‘‘Picual’’) 25 10.68B 0.29A 4.76A 239A
30 11.83BC 0.30A 3.00A 238A
35 13.47BC 0.38AB 5.21A 199A
40 15.44C 0.51B 7.19A 121B
a) Values are means of four replicates. Different letters indicate significant differences between treatments, p � 0.05.
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