The world leader in serving science Proprietary & Confidential

Brett Dalman, B.sc

Senior Bioscience Account Manager

Overview of Plant Solutions

2 Proprietary & Confidential

Overview of Product Portfolio

Agricultural biotechnology: www.thermofisher.com/agbio

3 Proprietary & Confidential

• MagMAX purification kits

• GeneJET purification kits

• MagJET purification kits

• PureLink purification kits

• Microbiome DNA purification

• KingFisher automated extraction system

• NanoDrop spectrophotometer

• Qubit fluorometer

• Nucleic acid purification: www.thermofisher.com/nap

Nucleic acid isolation and purification

4 Proprietary & Confidential

Nucleic acid isolation and purification – Key Products

KingFisher Flex Purification System

NanoDrop One

Spectrophotometer

MagMAX Plant DNA Isolation Kit

Plant RNA Reagent

5 Proprietary & Confidential

• Protein extraction

• Protease and phosphatase inhibitors

• Zeba desalting resins and columns

• Antibody purification

• Fusion purification

• Biotin affinity purification

• Protein immobilization and purification

• Protein biology: www.thermofisher.com/proteinbiology

Protein isolation and purification

6 Proprietary & Confidential

Protein isolation and purification – Key Products

P-PER Plant Protein Extraction Reagent

Dynabeads His-Tag Isolation and Pulldown

Pierce Protein Concentrators

HisPur Ni-NTA Agarose Resin

7 Proprietary & Confidential

• Western blotting

• BCA protein assays

• ELISA

• Mass spectrometry: sample preparation and quantification

• Antibodies

• E-PAGE high-throughput precast gel system

• iBright imaging system

• Protein biology: www.thermofisher.com/proteinbiology

Protein analysis

8 Proprietary & Confidential

Protein analysis – Key Products

E-PAGE Protein Gels

iBright FL1000 Imager

Coming soon

Custom ELISA

Western Workflow

9 Proprietary & Confidential

• SimpliAmp thermal cycler

• ProFlex thermal cycler

• Automated thermal cycler

• Platinum Taq DNA polymerase

• Direct PCR kits and master mixes

• Oligos

• Reverse transcriptases

• E-Gel electrophoresis system

• Molecular biology applications:

www.thermofisher.com/thermoscientificmolbio

PCR, RT-PCR, and electrophoresis

10 Proprietary & Confidential

PCR, RT-PCR, and electrophoresis – Key Products

E-Gel Agarose Gels Superscript IV cDNA synthesis kit

Phire Plant Direct PCR Mastermix

Platinum Superfi PCR Mastermix

11 Proprietary & Confidential

PCR, RT-PCR, and electrophoresis – Key Products con’t

Automated Thermal Cycler

(ATC) 96 and 384 well system

ProFlex

PCR System SimpliAmp

Thermal Cycler

Veriti Thermal

Cycler

E-Gel system for routine DNA or RNA

electrophoresis

(8 to 16 samples per gel)

E-Gel system for high-throughput

DNA screening

(48 to 96 samples per gel)

12 Proprietary & Confidential

• Plasmid purification kits

• Competent cells

• Gateway cloning

• TOPO cloning

• Seamless cloning

• Restriction enzymes

• Bac-to-Bac expression system

• PichiaPink expression system

• BL21 E. coli expression system

• Cloning: www.thermofisher.com/cloning

Cloning and protein expression

13 Proprietary & Confidential

Cloning and protein expression – Key Products

Gateway Recombination Cloning Technology

Anza Restriction Enzymes

14 Proprietary & Confidential

• GeneArt Strings DNA fragments

• GeneArt gene synthesis

• GeneArt Genes-to-Proteins service

• RNAi technologies

• CRISPR-Cas9 genome editing

• TALEN-based genome editing

• Transfection reagents

• Neon transfection system

• Genome editing: www.thermofisher.com/genomeediting

Synthetic biology, genetic engineering, and services

15 Proprietary & Confidential

Synthetic biology, genetic engineering, and services - Key Products

• ElectroMAX™ A. tumefaciens LBA4404 Cells

• Neon Transfection System

• GeneArt PerfectMatch TALEN

• GeneArt Platinum Cas9 Nuclease

• GeneArt™ Precision gRNA Synthesis Kit

• Custom Guide RNA

• GeneArt Genomic Cleavage Detection Kit

• Genome sequencing

• SNP genotyping

• Gene expression analysis

16 Proprietary & Confidential

• EVOS imaging systems

• Attune NxT flow cytometer

• Countess II cell counters

• High-content screening

• Click-iT Plus reagents

• Antibodies

• Alexa Fluor dyes

• ReadyProbes reagents

• Cellular imaging and analysis: www.thermofisher.com/cellimaging

Cellular imaging and analysis

17 Proprietary & Confidential

Cellular imaging and analysis – Key Products

CellInsight™ CX5 High Content Screening (HCS) Platform Countess II FL Automated Cell Counter

Attune NxT Flow Cytometer EVOS Cell Imaging Systems

18 Proprietary & Confidential

• QuantStudio real-time PCR systems

• Ion Torrent sequencers

• Capillary electrophoresis

• SuperScript VILO master mixes

• TaqMan real-time PCR master mixes

• TaqMan Sample-to-SNP kit

• TaqMan Assays

• SYBR Green master mixes

• Genotyping by sequencing

• Metagenomics

• Microarrays

• Real-time PCR: www.thermofisher.com/taqman

Real-time PCR and sequencing

19 Proprietary & Confidential

Real-time PCR and sequencing – Key Products

Applied Biosystems QuantStudio real-time

PCR and digital PCR system family

Ion S5 Sequencing Systems

TaqMan Assays

PowerUp Sybr Mastermix

and

TaqMan GTXpress Master Mix

20 Proprietary & Confidential

• Agriculture resource guide

• Tools for protein research

• Cell and protein isolation technical handbook

• The Molecular Probes™ Handbook

• Real-time PCR handbook

• Molecular biology workflow solutions

• Protein expression handbook

• Transfection and genome engineering

• Molecular Probes™ flow cytometry products and resource guide

• Thermo Scientific™ Pierce™ products for mass spectrometry sample

preparation

• For webinars on plant research, go to thermofisher.com/plantwebinars

Resources