ozone testing on honey bee equipment
DESCRIPTION
Ozone Testing on Honey Bee Equipment. By Elmer James Martin James Karla James Shelton Of Slide Ridge Honey Mendon, Utah June 9, 2008. Assisted by:. O3 Company, Aberdeen, Idaho USDA-ARS Bee Research Lab, Beltsville Utah Dept. of Agriculture, Salt Lake City - PowerPoint PPT PresentationTRANSCRIPT
1
Ozone Testing on Honey Bee Equipment
By
Elmer James
Martin James
Karla James Shelton
Of
Slide Ridge Honey
Mendon, Utah
June 9, 2008
2
Assisted by:
• O3 Company, Aberdeen, Idaho
• USDA-ARS Bee Research Lab, Beltsville
• Utah Dept. of Agriculture, Salt Lake City
• USDA-ARS Pollinating Insect Research Unit, Logan, Utah
• Ozone Research Equipment, Akron, Ohio
3
Lab Facilities
• Small Test Chamber at Ozone Research Equipment in Akron, Ohio
• Lab Testing at USDA-ARS Beltsville Bee Lab in Beltsville, Maryland.
• Lab Testing at Utah Dept of Agriculture in Salt Lake City, Utah
• Lab Assistance from USDA-ARS Bee Lab in Logan, Utah.
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Full Scale Field Testing
• Ozone room constructed at Slide Ridge Honey in Mendon, Utah
• Size: 20 ft. X 15 ft. X 10 ft.• Large ozone generator unit 2050-088,
double chamber type manufactured by O3 company in Aberdeen, Idaho.
• Monitor with range 0 to 2000 PPM provided by O3 company.
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Ozone Room
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Ozone Room Sealed
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Ozone Generator
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Ozone Generator Inside
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Ozone Monitor
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Ozone Test Start up Number 1Ozone Start-Up Number 1
0
20
40
60
80
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120
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268.
200
285.
000
310.
200
357.
000
367.
800
394.
800
408.
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536.
400
666.
000
670.
200
672.
000
676.
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1278
.000
1336
.800
1380
.000
Minutes
PP
M
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Ozone Test Start up Number 2Ozone Start up number 2
0
50
100
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25.8
00
33.6
00
96.0
00
154.
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175.
200
203.
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258.
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278.
400
360.
000
419.
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445.
800
648.
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671.
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673.
000
Minutes
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Ozone Test Start up Number 3Ozone Start up Number 3
0.00
100.00
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400.00
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700.00
800.00
0.00
4.00
12.0
013
.00
15.0
017
.00
25.0
047
.00
54.0
055
.00
90.0
0
126.
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175.
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181.
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209.
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Minutes
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Ozone Test Start up Number 4Ozone Start Up Number 4(End of Batch 301)
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0.000
7.000
22.0
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35.0
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72.0
00
121.
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145.
000
175.
000
254.
000
324.
000
491.
000
645.
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1120
.000
1312
.000
1510
.000
1539
.000
1582
.000
1791
.000
1923
.000
Minutes
PP
M
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Ozone Test Run Number 2Run Number 2 (150 Boxes w/Frames)
0
500
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0.000
4.800
11.4
00
36.6
00
130.
800
173.
400
207.
000
273.
000
325.
800
529.
200
1096
.200
1140
.600
1150
.800
1698
.000
Minutes
PP
M
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Ozone Test Run Number 3Run Number 3 (216 Boxes W/Frames)
0
200
400
600
800
1000
1200
1400
1.800
82.8
00
868.
800
1144
.800
1215
.600
1381
.800
1602
.600
1606
.200
1672
.200
1793
.400
1949
.400
2019
.000
2052
.600
2347
.800
3057
.000
3179
.400
3324
.000
3526
.800
Minutes (58.8 Hours Run time)
PP
M o
f O
zon
e
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Ozone Test Run Number 4Test Run Number 4 (221 W/Comb, 127 W/O, 12 Pallets)
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800
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1200
1400
0.000
90.6
00
244.
200
364.
200
405.
600
466.
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532.
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609.
000
625.
800
718.
800
730.
800
882.
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988.
800
1538
.400
1741
.200
1869
.600
1984
.200
2063
.400
2272
.200
Minutes (37.9 Hours)
Ozo
ne
in P
PM
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Part of Ozone Test Run Number 4 (USU Data Logger)
Part of Test Run #4 (USU Data Logger)
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04/1
7/08
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30:0
0.0
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7/08
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40:0
0.0
04/1
7/08
09:
50:0
0.0
04/1
7/08
11:
00:0
0.0
04/1
7/08
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10:0
0.0
04/1
7/08
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20:0
0.0
04/1
7/08
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30:0
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04/1
7/08
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40:0
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04/1
7/08
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50:0
0.0
04/1
7/08
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00:0
0.0
04/1
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10:0
0.0
04/1
7/08
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20:0
0.0
04/1
7/08
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30:0
0.0
04/1
7/08
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40:0
0.0
04/1
7/08
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50:0
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8/08
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00:0
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10:0
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20:0
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30:0
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40:0
0.0
04/1
8/08
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50:0
0.0
Time
Ozo
ne
in P
PM
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Ozone Test Run Number 5Test Run Number 5 (SRH Data Record)
0
500
1000
1500
2000
2500
Minutes (54.2 Hours)
Ozo
ne
in P
PM
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Ozone Test Run Number 5 (USU Data Logger Record)
Test Run Number 5 (USU Data Logger)
0
500
1000
1500
2000
2500
Time
PP
M
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Observations
• Pesticides residue destroyed.
• Herbicides residue destroyed.
• Wax moth destroyed, inactive remains.
• Gasoline residue destroyed on outer surface during run #5 on a customer’s Hives.
• Comb readily accepted by Honey Bees.
• Dry Rot fungus may be stopped.
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Observations Con’t.
• Foundation purchased several years ago was never acceptable to our Honey Bees. Now that this foundation has been submitted to Ozone they seem to readily move on to it and start building comb.
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Items not Subdued
• FOULBROOD
• More testing required & with different techniques.
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State of Utah Lab Tests
• 2008-8 Control Sample of Beeswax & Pollen with no Pesticides applied after Ozone treatment.
• 2008-9 Beeswax & Pollen with Fluvalinate applied and then treated with Ozone.
• 2008-10 Beeswax & Pollen with Amitraz applied and then treated with Ozone.
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State of Utah Sample 2008-8
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State of Utah Sample 2008-9
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State of Utah Sample 2008-10
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Wax Moth Damage
• Comb with severe wax moth damage has never been acceptable to our Honey Bees, therefore these frames were disposed of in our operation.
• Same severe damaged comb, Ozone treated, is very acceptable, the bees move right on to it and starting cleaning out the cells for reuse. Refuse dumped on bottom board by the bees, tip hive and scrap out.
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Typical Wax Moth Damage
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Typical Damaged Frame After Ozone Treatment
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Drying out Equipment
• The Ozone process seems to dry out all of the equipment, boxes, frames, comb, pallets, etc.
• O3 CO theorizes that dry air input as the carrier for the Ozone gas is absorbing the moisture from the equipment.
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Room Pressure Exhaust With Charcoal Filter
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Air Circulation In Ozone Room, NO Rubber or Urethane Belts
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Air Circulation in Ozone Room Requires Direct Drive Blower
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Coding of Equipment Processed Through Ozone
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Coded Hive Box & Frames
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Restack Hive Boxes to Allow Better Air Circulation
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Locate Ozone Sensor Near Top of Equipment Being Processed
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Depressurize ducting, route outside of building
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Cost
• Ozone generator $3,000.00 per month lease.
• Electric power $194.94.
• Frames processed 8,880.
• Labor $0.00.
• Cost per brood frame = $0.36 each.
• Not included, improvement to boxes, pallets, lids, etc.
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End Result of Ozone Treated Equipment. (30 Day Nuc)
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Honey Bee Care
• Bees shown in slides have been treated for Mites, Nosema, Foulbrood, and Chalkbrood.
• Their diet has been supplemented with Bee-Pro & other additives.
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Conclusions by Slide Ridge Honey
• All “Dead Out Hives” should be processed in an Ozone Chamber before attempting to raise Honey Bees in them.
• We consider placing Honey Bees in unprocessed “Dead Out Hives”, a waste of time and money.
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Conclusions Continued
• Why does Ozone Treatment Work?
• This was the first full scale Ozone Test under field conditions, and we know a number of items that are being destroyed, however we may have wiped out other detrimental elements that need to be known and requires further Lab Research.
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Comments
• We wish to acknowledge the USU Bee Lab and their staff for coming up with the idea of using Ozone to purify Bee Hives, and now that we have proven that the idea is economically feasible by Bee Keepers at the commercial level, we encourage USU Bee Lab to enlarge this research program at the earliest opportunity.
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Comments Continued
• We believe that with Ozone Processing and proper nutrition, CCD may disappear from the Bee Keeping operation.
• We believe that our two biggest problems in bee keeping are: pesticides/herbicides being returned to the hive & poor honey bee nutrition.
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Factor
• We need for the Lab to establish a factor that relates:Time in Ozone chamber to PPM of Ozone
This would allow us to cut cost on power consumption and/or equipment size.
We are also of the opinion that the Logan Bee Lab is in the best position to conduct this Research.
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Distribution
• USDA-ARS Bee Lab
5310 Old Main Hill
Room 253
Logan, Utah 84322-5310
Dr. Rosalind James
Craig Huntzinger
Ellen Klinger
• State of Utah
Dept. of Agriculture & Food
350 N. Redwood Road
Salt Lake City, Utah 84114-6500
Danielle Downey
Dr. David Clark
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Distribution Con’t.
• Beltsville Bee Lab
Room 211
10300 Baltimore AV.
Building 476 BARC-East
Beltsville, MD 20705
Bart Smith
Jeffery Pettis
• O3 Manufacturing
2775 West 1800 South
Aberdeen, Idaho 83210
Lynn Johnson
208-397-3033
208-220-1288 cell
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Distribution Con’t.
• Ozone Research Equipment Company
221 Beaver Street
Akron, Ohio 44304
Ron Brainard
800-742-8535
• Bee Keepers:
Newswander Brothers Honey
Pendell Apiaries
Homan McMaster Apiaries
Mike Brockman
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Distribution Con’t.
• Suppliers:
Mann Lake Ltd.
Stuart Volby
501 1st. St. South
Hackensack, MN
800-880-7694