P15080: Flow Culture SystemMulti-Disciplinary Senior Design

Problem Definition Review

September 11, 2014Collin Burkhart – Lead Mechanical Engineer

Katelyn Busse – Lead Biomedical EngineeringMichelle Garofalo – Project Manager

Robert Repetti – Lead EDGE FacilitatorMorgan Stoessel - Treasurer

Sarah Tran – Note Taker

Agenda

● Project Background● Project Statement● Stakeholders● Use Scenarios ● Customer Needs and

Constraints● Engineering

Requirements

● House of Quality● Benchmarking ● Top Risks● Deliverables ● Project Plan● Questions

Project Background

● Cell cultureo What is it?

● Motivation for this project

Problem Statement

● Current Statuso 2D - Statico 3D - Static

● Current Limitationso Static Conditionso No current deviceo Micro vs. Macrofluidics

● The Main Objective

Stakeholders ● Dr. Jennifer Bailey● Biomedical engineering students taking

Advanced Cell Culture Class● Dr. Dan Phillips● Biomedical Engineering Department● Rochester Institute of Technology● MSD Team (and potential future MSD Teams)

Communication Plan● Follow up meeting with Dr. Bailey every two

weeks● E-mail customer with questions and

concerns at any time● Discuss budget with Dr. Phillips● Reach out to Dr. Man and Dr. Gaborski for

endothelial expertise

Use Scenario Flow Culture Lab Experiment to Grow Endothelial Cells

RIT

Instructor

Student(s)

Advanced Cell Culture class

offered

Retrieves flow culture system from storage

Inspects components for damage

Has students perform

experiment

Assembles components

of system

Sterilizes empty system in autoclave

Transport system to

biosafety hood

Replaces damaged

components

Damaged?

Yes

No

Use Scenario Flow Culture Lab Experiment to Grow Endothelial Cells

RIT

Instructor

Student(s)Add sterile

media to media reservoir

Seed growth plate

Add seeded growth plate to

growth chamber

Transfer to incubator

Power pump and sensory equipment

Activate pump to initiate

media flow

Ensure flow is continuous and free of bubbles

Use Scenario Flow Culture Lab Experiment to Grow Endothelial Cells

RIT

Instructor

Student(s)Adjust pump flow rate for

desired shear stress

Adjust incubator for desired pH

value

Adjust incubator temperature as

desired

Allow cells to grow

Cycle through new media

every 3-4 days

Disconnect growth chamber

to view cells

Reconnect growth chamber

after viewing

Use Scenario Flow Culture Lab Experiment to Grow Endothelial Cells

RIT

Instructor

Student(s) End experiment after two weeks

Deactivate pump

Cut power to pump and

sensory equipment

Transfer system to biosafety

hood

Disconnect growth

chamber

Drain remaining media from

system

Observe cells in growth chamber on microscopes

Use Scenario Flow Culture Lab Experiment to Grow Endothelial Cells

RIT

Instructor

Student(s)

Advanced Cell Culture class

ends

Students have completed experiment

Drain growth chamber

Autoclave all equipment

Return equipment to

instructor

Returns equipment to

storage

Process repeats for additional experiments

Customer Requirements

Importance: 1=Essential, 2=Customer Want, 3=Preference

Essential Customer Requirements

Sterility, Temperature, pH

Constraints

● It is assumed that the flow culture system will be using only animal endothelial cell lines

● This system may need to sit in an incubator to control temperature, media pH, and relative humidity

● Chamber that holds cells needs to fit on defined microscope stages

● System must be sterilized by autoclave● Budget of ~$1550

Engineering Requirements

Importance: 1=Essential, 2=Customer Want, 3=Preference

Essential Engineering Requirements

Essential Engineering RequirementsDescription and Tests

House of Quality

● Every customer requirement has been satisfied by at least one engineering requirement.

● Diagram can be found at the following location on EDGE:http://edge.rit.edu/edge/P15080/public/Problem%20Definition%20Documents/Additional%20Documents P15080_House_of_Quality document

Benchmarking● Perform cell culture in BME Lab with Dr.

Bailey● Research and contact representatives of

current companies that market cell culture flow devices

● Confer with Dr. Gaborski and Dr. Man

Top RisksBudget: ● Materials too expensive

Schedule: ● Team member delays● Unexpected labor hours

Technical:● Unable to keep materials sterile● Material malfunction● Pump inadequacies● Lack of knowledge in electrical engineering spectrum for sensor

configuration

Project Plan

Deliverables for Next Cycle

● Functional Decomposition 9/18● Concept Generation 9/23● Morph Table 9/23● Function/Engineering Analysis 9/23● Feasibility Analysis 9/25● System Design Review 9/30 or 10/2

Questions,Comments,

orConcerns?

Thank you!