pathogens agrobacterium tumefaciens agrobacterium rhizogenes pseudomonas syringeae pseudomonas...

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Pathogens Agrobacterium tumefaciens Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes nematodes Symbionts N-fixers Endomycorrhizae Ectomycorrhizae

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Pathogens Agrobacterium tumefaciens Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes nematodes Symbionts N-fixers Endomycorrhizae Ectomycorrhizae. Mineral Nutrition Macronutrients: CHOPKNSCaFeMg - PowerPoint PPT Presentation

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Page 1: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Pathogens• Agrobacterium tumefaciens • Agrobacterium rhizogenes• Pseudomonas syringeae• Pseudomonas aeruginosa• Viroids• DNA viruses• RNA viruses• Fungi• oomycetes• nematodes

Symbionts• N-fixers• Endomycorrhizae• Ectomycorrhizae

Page 2: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Mineral NutritionMacronutrients: CHOPKNSCaFeMg

• Ca: signaling, middle lamella, cofactor• Fe: cofactor• Mg: cofactor• mobile in plant, so shows first in old leaves

Page 3: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Mineral NutritionMicronutrients: BNaCl others include Cu, Zn, Mn

• B: cell elongation. NA metabolism• Na: PEP regeneration, K substitute• Cl: water-splitting, osmotic balance• Cu: cofactor• immobile in plant, so shows first in young leaves

Page 4: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Mineral NutritionSoil nutrients•Amounts & availability vary•Many are immobile, eg P, Fe

Page 5: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Mineral NutritionNutrients in soil•Plants alter pH @ roots to aid uptake• Also use symbionts

• Mycorrhizal fungi help: especially with P

Page 6: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Mineral NutritionAlso use symbionts•Mycorrhizal fungi help: especially with P• P travels poorly: fungal hyphae are longer & thinner

• Fungi give plants nutrients• Plants feed them sugar

• Ectomycorrhizae surround root: only trees, esp. conifers

Page 7: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Mineral NutritionEctomycorrhizae surround root: trees•release nutrients into apoplast to be taken up by rootsEndomycorrhizae invade root cells: Vesicular/Arbuscular

• Most angiosperms, especially in nutrient-poor soils

Page 8: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

RhizosphereEndomycorrhizae invade root cells: Vesicular/Arbuscular

• Most angiosperms, especially in nutrient-poor soils• May deliver nutrients into symplast• Or may release them when arbuscule dies

Page 9: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

RhizosphereEndomycorrhizae invade root cells: Vesicular/Arbuscular

• Most angiosperms, especially in nutrient-poor soils• Deliver nutrients into symplast or release them

when arbuscule diesAlso find bacteria, actinomycetes, protozoa associated with root surface = rhizosphere

Page 10: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

RhizosphereAlso find bacteria, actinomycetes, protozoa associated with root surface = rhizosphere• Plants feed them lots of C!

Page 11: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

RhizosphereAlso find bacteria, actinomycetes, protozoa associated with root surface = rhizosphere• Plants feed them lots of C!• They help make nutrients available

Page 12: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

RhizosphereAlso find bacteria, actinomycetes, protozoa associated with root surface = rhizosphere• Plants feed them lots of C!• They help make nutrients available• N-fixing bacteria supply N to many plant spp

Page 13: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

N assimilation by N fixersExclusively performed by prokaryotesDramatically improve the growth of many plants

Page 14: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

N assimilation by N fixersExclusively done by prokaryotesMost are free-living in soil or water

Page 15: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

N assimilation by N fixersExclusively done by prokaryotesMost are free-living in soil or waterSome form symbioses with plants

Page 16: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

N assimilation by N fixersExclusively done by prokaryotesMost are free-living in soil or waterSome form symbioses with plantsLegumes are best-known, butmany others including mosses,ferns, lichens

Page 17: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

N assimilation by N fixersExclusively done by prokaryotesMost are free-living in soil or waterSome form symbioses with plantsLegumes are best-known, butmany others including mosses,ferns, lichensAlso have associations where N-fixers form films on leaves orroots and are fed by plant

Page 18: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

N assimilation by N fixersExclusively done by prokaryotesAlso have associations where N-fixers form films on leaves orroots and are fed by plantAll must form O2-free environment for nitrogenase

Page 19: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

N assimilation by N fixersAll must form O2-free environment for nitrogenaseO2 binds & inactivates electron-transfer sites

Page 20: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

N assimilation by N fixersO2 binds & inactivates electron-transfer sitesHeterocysts lack PSII, haveother mechs to lower O2

Page 21: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

N assimilation by N fixersHeterocysts lack PSII, haveother mechs to lower O2 Nodules have specialstructure + leghemoglobinto protect from O2

Page 22: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nodule formationNodules have special structure + leghemoglobin to protect

from O2 Bacteria induce the plant to form nodules

Page 23: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nodule formationBacteria induce the plant to form nodules1. Root hairs secrete chemicals that attract N-fixers

Page 24: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nodule formationBacteria induce the plant to form nodules1. Root hairs secrete chemicals that attract N-fixers2. Bacteria secrete Nod factors that induce root hair to

coil up. Nod factors determine species-specificity

Page 25: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nodule formation1. Root hairs secrete chemicals that attract N-fixers2. Bacteria secrete Nod factors that induce root hair to

coil up. Nod factors determine species-specificity3. Nod factors induce degradation of root cell wall

Page 26: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nodule formation3. Nod factors induce degradation of root cell wall4. Plant forms "infection thread"=internal protusion of

plasma membrane that grows into cell5. When reaches end of cell bacteria are released into

apoplast and repeat the process on inner cells

Page 27: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nodule formation5. When reaches end of cell bacteria are released into

apoplast and repeat the process on inner cells6. Cortical cells near xylem form a nodule primordium

Page 28: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nodule formation5. When reaches end of cell bacteria are released into

apoplast and repeat the process on inner cells6. Cortical cells near xylem form a nodule primordium7. When bacteria reach these cells the infection thread

breaks off, forming vesicles with bacteria inside

Page 29: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nodule formation7. When bacteria reach these cells the infection thread

breaks off, forming vesicles with bacteria inside8. Vesicles fuse, form the peribacteroid membrane and

bacteria differentiate into bacteroids.

Page 30: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nodule formation8. Vesicles fuse, form the peribacteroid membrane and

bacteria differentiate into bacteroids.9. Plant cells differentiate into nodules

Page 31: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nodule formation8. Vesicles fuse, form the peribacteroid membrane and

bacteria differentiate into bacteroids.9. Plant cells differentiate into nodules: have layer of cells

to exclude O2 & vasculatureto exchange nutrients

Page 32: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nodule formationPlant cells differentiate into nodules: have layer of cells to

exclude O2 & vasculature to exchange nutrientsComplex process that is difficult to engineer: 21 non-

legume plant genera have N-fixers

Page 33: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nitrogen fixationN2 + 8H+ + 8e− + 16 ATP → 2NH3 + H2 + 16ADP + 16 Pi

Catalysed by nitrogenase, a very complex enzyme!

Page 34: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nitrogen fixationN2 + 8H+ + 8e− + 16 ATP → 2NH3 + H2 + 16ADP + 16 Pi

Catalysed by nitrogenase, a very complex enzyme!Also catalyzes many other reactionsUsually assayed by acetylene reduction

Page 35: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nitrogen fixationN2 + 8H+ + 8e− + 16 ATP → 2NH3 + H2 + 16ADP + 16 Pi

Usually assayed by acetylene reductionSequentially adds 2 H per cycle until reach NH3

Page 36: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nitrogen fixationSequentially adds 2 H per cycle until reach NH3

May then be exported to cytosol & assimilated by GS/GOGAT or assimilated inside bacteroid

Page 37: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nitrogen fixationSequentially adds 2 H per cycle until reach NH3

May then be exported to cytosol & assimilated by GS/GOGAT or assimilated inside bacteroidAre then converted to amides or ureides& exported to rest of plant in the xylem!

Page 38: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nutrient uptakeMost nutrients are dissolved in water

Page 39: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nutrient uptakeMost nutrients are dissolved in water

• Enter root through apoplast until hit endodermis

Page 40: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nutrient uptakeMost nutrients are dissolved in water

• Enter root through apoplast until hit endodermis• Then must cross plasma membrane

Page 41: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Crossing membranesA) Diffusion through bilayer B) Difusion through protein poreC) Facilitated diffusionD) Active transportE) Bulk transport

1) Exocytosis2) Endocytosis

Selective

Active

Page 42: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nutrient uptakeThen must cross plasma membrane

• Gases, small uncharged & non-polar molecules diffuse

Page 43: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nutrient uptakeThen must cross plasma membrane

• Gases, small uncharged & non-polar molecules diffusedown their ∆ [ ]• Important for CO2, auxin & NH3 transport

Page 44: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Nutrient uptakeThen must cross plasma membrane

• Gases, small uncharged & non-polar molecules diffusedown their ∆ [ ]• Polar chems must go through proteins!

Page 45: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Selective Transport1) Channelsintegral membrane proteins with pore that specific ions diffuse through

Page 46: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Selective Transport1) Channelsintegral membrane proteins with pore that specific ions diffuse through• depends on size & charge

Page 47: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Channelsintegral membrane proteins with pore that specific ions diffuse through• depends on size & charge• O in selectivity filter bindion (replace H2O)

Page 48: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

Channelsintegral membrane proteins with pore that specific ions diffuse through• depends on size & charge• O in selectivity filter bindion (replace H2O)• only right one fits

Page 49: Pathogens Agrobacterium tumefaciens  Agrobacterium rhizogenes Pseudomonas syringeae Pseudomonas aeruginosa Viroids DNA viruses RNA viruses Fungi oomycetes

ChannelsO in selectivity filter bindion (replace H2O)• only right one fits• driving force?electrochemical