patronin regulates micro tubule network
TRANSCRIPT
Patronin Regulates thePatronin Regulates theMicrotubule Network byMicrotubule Network by
Protecting Microtubule Minus Protecting Microtubule Minus EndsEnds
Sarah S. Goodwin and Ronald D. ValeCell 143, 263–274, October 15, 2010
Tubulin assembles into polar filaments with dynamic plus and minus ends.
However, in living cells, most microtubule minus ends are static.
Goodwin and Vale identify Patronin as a protein that stabilizes minus ends and protects them from depolymerization in vivo.
They further show that purified Patronin binds selectively to minus ends and shields them from a microtubule depolymerase, Kinesin-13.
Patronin contributes to • proper organization of the microtubule cytoskeleton and • formation of the mitotic spindle,
indicating that these structures are regulated by competing actions of destabilizing and stabilizing proteins acting on minus ends
• Patronin protects microtubule minus ends from depolymerases in vivo
• Without Patronin, microtubule organization is defective in interphase and mitosis
• In vitro Patronin can protect minus ends from Kinesin-13-catalyzed depolymerization
• Similar to plus ends, stabilizing and destabilizing proteins regulate minus ends
Brian P. O'Rourke David J. Sharp Microtubule Dynamics: Patronin, Protector of the Minus EndCurrent Biology, Volume 21, Issue 2, R77-R79, 25 January 2011
