personalising crispr genome editing || edward perello || disruptor stories

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@EdwardPerello | @DesktopGenetics © Desktop Genetics Ltd. 2016 An Illumina- backed company PERSONALISING CRISPR GENOME EDITING August 2016 Edward Perello| CBO & Founder

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Page 1: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics© Desktop Genetics Ltd. 2016 An Illumina-backed company

PERSONALISING CRISPRGENOME EDITING

August 2016

Edward Perello| CBO & Founder

Riley Doyle
Insert GUIDE PICKER GIF
Page 2: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

DESKTOP GENETICSHELPING YOU MANIPULATE GENOMES

LONDONEST. 2012

BIOINFORMATICS TOOLS

GENE EDITING SERVICES

BACKED BY LARGEST SEQUENCING PROVIDER

Edward Perello
Keep it simple. Lay groundwork for complexity. Credential company.
Victor Dillard
you can now add Twist and Vectalys by the way
Page 3: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

TWO PATHS TO DESKTOP GENETICSPRODUCTS & SERVICES DATA

Edward Perello
This is what we sell. All of this creates data.
Page 4: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics 4

TEACH AI TO PERFORM GENETIC SURGERYMACHINE INTELLIGENCE IS THE ONLY WAY TO EDIT THE GENOME SAFELY

Edward Perello
The data feeds the machine. INTRODUCE concept of AI for genomic surery.INFORM people that AI is the only way to do genome surgery
Page 5: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

AGENDA

1. Brief intro to CRISPR

2. Our story so far

3. Taking CRISPR to the clinic

Page 6: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics© Desktop Genetics Ltd. 2016 An Illumina-backed company

Editing the Genome with CRISPR

Page 7: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

GENE THERAPY TACKLES DISEASESCRISPR is used to modify the genome of a patient’s cells

Page 8: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

CRISPR OVERVIEWProgrammable two-component system

CAS9 NUCLEASE

RNACOMPONENT

Page 9: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

CRISPR OVERVIEWProgrammable two-component system

CAS9 NUCLEASE

RNACOMPONENT

VARIABLE20 BP GUIDE RNA

CONSTANTREGION

Page 10: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

CRISPR OVERVIEWProgrammable two-component system

CAS9 NUCLEASE

RNACOMPONENT

ACTIVE RNA-GUIDED CAS9

COMPLEX

Page 11: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

CRISPR OVERVIEWMolecular find and replace for DNA

Source: McGovern Brain Institute Youtube Channel

Victor Dillard
who is the audience? I feel like a nice comparison would be to put side by side the CRISPR system and a pair of scissors for example.
Page 12: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

WHY EDIT GENOMES?Research & development → Clinical interventions

- Degenerative blindness- Custom disease models

- Humanization of heart valves- Swine fever resistance

- HIV eradicated in vitro- Immuno-oncology

- Clinical trials cured cancer - Clinical trials cured HIV

Page 13: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

WHY START A BUSINESS IN THIS SPACE?Huge biotechnology, life science and clinical prospects

75M PATIENTS3M RESEARCH EXPERIMENTS

Page 14: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics© Desktop Genetics Ltd. 2016 An Illumina-backed company

Our Story

Page 15: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

2011 – MEETING AT CAMBRIDGEMPHIL BIOSCIENCE ENTERPRISE DEGREE

Page 16: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

2012 – SYNTHBOT, CUTEC, SEEDTHE BIOTECH NESPRESSO

Page 17: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

2013 – AUTOCLONE, TSB , RAISEHARDWARE OUT, SOFTWARE IN

Page 18: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

2014 – HORIZON CRISPR TOOL & TSBINTERNAL CRISPR R&D TOOL

Page 19: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

2014 - GUIDEBOOKCO-BRANDED CRISPR DESIGN & SALES PORTAL

Page 20: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

2015 – DESKGEN, CLASS, PARTNERSUNITING & OPTIMISING OUR TOOLSET

Page 21: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

CRISPR EXPERIMENTS MODE, RICHER UI2015 – DESKGEN, CLASS, PARTNERS

Page 22: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

2015 – DESKGEN, CLASS, PARTNERSPARTNERSHIPS MAKE LITERAL DESKTOP GENETICS POSSIBLE

Page 23: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics© Desktop Genetics Ltd. 2016 An Illumina-backed company

Taking CRISPR into the clinic

Page 24: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

INTERNATIONAL ENDEAVOURCRISPR is a global phenomenon

Page 25: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

2016 – GUIDE PICKER, NGS, BEYONDMULTIDIMENSIONAL GUIDE SELECTION

Page 26: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

2016 – GUIDE PICKER, NGS, BEYONDUSING BIOLOGICAL DATA TO TAKE OUR AI FURTHER

Page 27: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

HUMANS VS. MACHINESMACHINES OUT-PERFORM MANUAL DESIGN

CRIS

PR S

ucce

ss R

ate

Page 28: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics 28

PERFORM GENETIC SURGERY WITH AIDesign CRISPR for every lab, teach our algorithms, go to the clinic

Edward Perello
Speak aspirationally about the mission. Make them feel it.
Page 29: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

LIFE AT DESKTOP GENETICSRecognition in tech, biology, and everything in between

Edward Perello
Remind them that its fun.MENTION THE TEAM AND HOW GREAT THEY ARE. Its not just a business.
Victor Dillard
add the LaBiotech award logo?
Page 30: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics

WE’RE HIRINGJoin us in Shoreditch; tell your friends!

Edward Perello
We have a kickass office. We are going to have a lot more events. We are ACTIVELY hiringMention the meetup I am hosting on the 14th. TELL people to speak to people they think might be suitable for us. INVITE people to speak with you.
Page 31: Personalising CRISPR Genome Editing || Edward Perello || Disruptor Stories

@EdwardPerello | @DesktopGenetics© Desktop Genetics Ltd. 2015© Desktop Genetics Ltd. 2

www.deskgen.com

[email protected]

@edwardperello