Group Members

•Adan Fatima Javed

•Sehrish Shoukat

•Zunaira Khalil

•Basharat Khan

•Syed Haider Ali

•Salimullah Khan Ayubi

PROJECT INSTRUCTOR: MISS ARISH NASEEM

Isolation and Identification of

Pesticide Degrading Bacteria in

Microbiology LAB

Pesticides Pesticides are substances meant for

preventing, destroying or mitigating any pest.

75%

14%

9%

2%Insecticides

Herbicides

Fungicides

Acricides

Types of pesticides/Classes breakup

Why Pesticides are used?

• Population pressure

• Food security

• Higher yield

• Province wise distribution

KPK 2%Sindh 8%Punjab 90%

Pesticide Use in Pakistan

Direct impact on humans

• Adversely impact on Central Nervous System (CNS)

• Toxic

Toxicity potential of Pesticides

Fate of Pesticide in Environment

Profenofos

Selected Pesticide-Profenofos

• Widely used in Pakistan

• Hazardous to health

• Among top ten used pesticides in Pakistan

– Used on cotton

– Commonly found as groundwater contaminants

– More effective

Reasons

• Microorganisms

• Use compound as carbon and energy source

Biodegradation

Methodology

Enrichment Acclimatization

Serial DilutionsSpreadingIncubation

Plate Count Isolation Characterization

Sampling

Methodology Steps

Media

Mineral Salt Media (MSM)

Use Distilled Water

For 100 ml of media

(0.15g/100 ml)

Autoclave

•Media

•Glass wares

e.g petri plates etc….

Soil Sample

Media

+

Orbital Shaker

For 2 Days

Enrichment

Acclimatization

Media

Concentration of

profenofos

100 ml 100 ml 100 ml 100 ml

control 50 ppm 100 ppm 150 ppm

Inoculum 1 ml 1 ml 1 ml

Serial Dilutions

Spreading

Incubation

For 2 days (48 hours) at 37 oC.

Plate Count

•Morphological Characterization

Colony Morphology (Microscopy) Cell Morphology (Through staining)

•Physiological Characterization

•Biochemical Characterization

•Molecular Characterization

Characterization

•Specific growth of pesticide degrading microorganisms

•Planning for isolation and Characterization in the next step

Conclusion

•Very good experience

•Applications of Microbiology in Environmental Engineering

•Would like to continue the research in the next semesters

Experience

Facilities in IESE Microbiology Lab

•Sterile Environment

•Well equipped

•Very cooperative and trained Staff

•Students/Researchers are always welcomed

Suggestions

• Separate lab for Bioremediation

• HEPA Filters

Professor Dr. Imran Hashmi

Miss Arish Naseem

Acknowledgement