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Petiveria alliacea L. Extract for the Treatment of Gastroesophageal Reflux Disease Edwin J. Alvarado

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Page 1: petiveria alliacea l

Petiveria alliacea L. Extract for the Treatment of

Gastroesophageal Reflux Disease

Edwin J. Alvarado

Page 2: petiveria alliacea l

Petiveria alliacea L.

Petiveria alliacea L., also known as Anamú or garlic weed, is a very influential plant in traditional medicine.

Proved to possess antibacterial1 and analgesic2 properties, among others.

Both contribute to the plant’s reputation for calming GERD symptoms.

Page 3: petiveria alliacea l

Question/Problem

Can the extract from the Petiveria alliacea L. affect the stomach’s pH or, more ideally, the stomach’s acid irrigation?

Page 4: petiveria alliacea l

Hypothesis

If the contents of the stomach are irrigated with the plant’s extract, the pH levels of the acid will increase, inhibiting the stomach’s acid irrigation over a long term exposition, sparing damage to the organs involved.

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Chemical Experiment

Prepare a solution of HCl, KCl and NaCl, among others, add consecutive doses of extract and record pH.

To determine if extract acts as a buffer solution.

If true, determine buffer region.

Page 6: petiveria alliacea l

In Vivo Experiment

Organism: Mice / Rats

Quantity: 20 per group (80 in total)

Variables: Presence of extract in ingestion

Presence of acid promoters

Prowess over time

Period: 4 weeks

Page 7: petiveria alliacea l

Experimental Groups

Group A

P. alliacea extract

No acid promoter foods

Group B

P. alliacea extract

Acid promoter foods

Group C

No P. alliacea extract

Acid promoter foods

Page 8: petiveria alliacea l

Control Group

Group D (NEGATIVE CONTROL)

No P. alliacea extract

No acid promoter foods

Page 9: petiveria alliacea l

Methodology

Strict, standardized diets, varying only aforementioned factors.

After each week, collect five specimens from each group and extract stomach contents.

Analyze pH, acid concentration, gastric component concentration.

Interpret results.

Page 10: petiveria alliacea l

Objectives

To determine if the extract “neutralizes” the stomach’s pH and in what capacity.

To determine if the extract affects the stomach’s acid irrigation and in what capacity.

To determine efficiency, if any, in abnormal stomach conditions.

To determine the efficiency, if any, of short term treatment versus long term.

Page 11: petiveria alliacea l

Goal

Provide those afflicted with GERD an alternative treatment that both relieves pain and controls the disease

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References

1. Kim S, Kubic R, Musah RA. Antibacterial and antifungal activity of sulfur-containing compounds from Petiveria Alliacea L. 2005. Available from: http://www.sciencedirect.com. Accessed: 2012 June 21.

2. Lopes-Martins RAB, Pegoraro DH, Woisky R, Penna SC, Sertié JAA. The anti-Inflammatory and analgesic effects of a crude extract of Petiveria Alliacea L. (Phytolaccaceae). 2004. Available from: http://www.sciencedirect.com. Accessed: 2012 June 21.

Page 13: petiveria alliacea l

Thank you all for your time and consideration.

Are there any questions, comments or suggestions?


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