pfdgat1-1 pfdgat1-2 atdgat1 rcdgat1 pfdgat1-1 pfdgat1-2 atdgat1 rcdgat1 pfdgat1-1 pfdgat1-2 atdgat1...

PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 Figure S1. Protein sequence alignment among PfDGAT1-1 (isotig11157), PfDGAT1-2 (isotig11156), AtDGAT1 (At2g19450), and RcDGAT1 (XP_002514132). Black shading indicates identical amino acids. Gray shading indicates similar amino acids and no shading indicates dissimilar amino acids. Dashes indicate gaps in alignment.

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PfDGAT1-1PfDGAT1-2AtDGAT1RcDGAT1

PfDGAT1-1PfDGAT1-2AtDGAT1RcDGAT1PfDGAT1-1PfDGAT1-2AtDGAT1RcDGAT1

Figure S1. Protein sequence alignment among PfDGAT1-1 (isotig11157), PfDGAT1-2 (isotig11156), AtDGAT1 (At2g19450), and RcDGAT1 (XP_002514132). Black shading indicates identical amino acids. Gray shading indicates similar amino acids and no shading indicates dissimilar amino acids. Dashes indicate gaps in align-ment.

PfDGAT2AtDGAT2RcDGAT2

Figure S2. Protein sequence alignment among PfDGAT2 (isotig19956), AtDGAT2 (At3g51520), and RcDGAT2 (XP_002528531).

Page 3: PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1

PfDGAT3AtDGAT3AhDGAT3

PfDGAT3AtDGAT3VADGAT3

PfDGAT3AtDGAT3AhDGAT3

Figure S3. Protein sequence alignment among PfDGAT3 (isotig08903), AtDGAT3 (At1g48300), and AhDGAT3 (AAX62735).

Page 4: PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1

PfPDAT1-1PfPDAT1-2

PDAT1-1F1

PDAT1-1R1

PDAT1-2F1

PDAT1-2R1

PfPDAT1-1PfPDAT1-2

Figure S4. C-terminal coding region of nucleotide sequence alignment between PfPDAT1-1 (isotig08780) and PfPDAT1-2 (isotig08781). Locations of qRT-PCR primer sequences for each gene are indicated with red arrows.

Page 5: PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1

Amino Acid Substitution per 100 residues

28.8

510152025

AtPDCT

PfPDCT

RcPDCT

PfPDCTAtPDCTRcPDCT

Figure S5. A. Phylogenetic tree showing relatedness among PfPDCT (isotig25038) (shaded), AtPDCT (At3g15820) RcPDCT (XP_002517643). B. Amino acid sequence alignments among the same three sequences. PfPDCT is partial cDNA sequence.

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