pharmacology designing experiment

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Pharmacology designing experiment

Effects of Diazepam

by oral administration with water, milk, grapefruit juice, alcohol, tea

Group member: Christine Tiffany Terry HomerTutor: Professor lv, Professor Xiong


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Presentation Outline

1. Introduction

1.1 case

1.2 questions2. Experiment Procedure3. Results (Data Analyses and Statistics)

aaa3.1. Sleeping latency

3.2. Sleeping duration

3.3. Open-field4. Conclusion


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Case

Alcohol +hypnotics=toxin？

Introduction


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Questions about administration of hypnotics are raised up

Why dose hypnotics with alcohol will produce toxin?

Isn’t it better to dose hypnotics with milk than with water?

Why it’s not proper to dose hypnotics with tea?

It’s reported that Grapefruit juice will sufficiently increase the hypnotic potency, true?

alcohol

tea


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Experiment procedures

1.grouping　 5　groups

5　mice　each　group　　　　　

2.giving　drug 　　　　　　

　 0.1ml/10g　solvent water　 grapefruit　juice alcohol tea milk

　 0.2ml/10g　drugdiazepa

mdiazepam

diazepam

diazepam

diazepam

　　　　　　　

3.observationawake　

spontaneously　　　　　

　 sleeping　latency 　　　　　

　 sleeping　duration 　　　　　

　 Open-field 　　　　　

　　　　　　　

　 giving　caffeine 　　　　　

　 sleeping　latency 　　　　　

　 sleeping　duration 　　　　　

　 open-field 　　　　　


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3.Results

3.1. Sleeping latency

20

5

25

25

8

0 5 10 15 20 25 30

water

al cohol

mi l k

tea

grapef rui t j ui ce

sl eepi ng l atency(mi ns)


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3.2. Sleeping duration

50

20

50

72

48

0 15 30 45 60 75

water

al cohol

mi l k

tea

j ui ce

sl eepi ng durati on(mi ns)

3.Results


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States Water Milk Tea Alcohol Grapefruit Juice

Aftergiving Diazepam

More and more sedative in 20mins

LongerSleepinglatency thanwater group

Longer sleepinglatency thanwater group

One of themis dead.Others fallasleep verysoon

Very active!Even doinggymnastics.

Sleepingcondition

Non—rapideyes-movement

No sufficientmovement

No sufficientmovement

unconscious Sleep like a log;

Very longlatency

Open FieldCondition

Walkingnormally

Walkingnormally

Walkingnormally

Very exciting;Walking fast

Stay still,Sometimeswalk slowly

Observation of mice’s action

3.Results


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3.3. Open-field

open-fi el d

23

66

99

33

49

166

1620

1

14

24

17

2 80102030405060708090

100

1 2 3record t i me(mi n)

grid

s

water al cohol mi l k tea grapef rui t j ui ce

Water vs. alcohol

Grapefruit juice,water and alcohol

3.Results


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4.Conclusions

(1)Oral administration with milk will lower the drug potency (2)Oral administration with Grapefruit juice will sufficiently increase the

hypnotic potency and cause adverse effect.

(3)Oral administration with tea will lower the hypnotic potency.

(4)Oral administration with alcohol will sufficiently increase the hypnotic potency and cause severe adverse effect.

pharmacology designing experiment

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