Photodynamic perforation of cell membrane on micro channel array toward intracellular technology

Advisor ： Cheng-Hsien Liu Reporter ： Yu-Sheng LinDate ： 2007.06.20

K. Iso1, T. K. Saito2, H. Muguruma3, H. Tabata2, S. Konishi1

1Ritsumeikan University, 2Osaka University 3Shibaura Institute of Technology, Japan MEMS 2007 pp.445~448

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Outline

IntroductionOperational procedureDesign and fabricationExperiment and resultsConclusion

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Ref: Wikipedia

Patch-clamp

Introduction

Cell Figure 1.

Schematic drawing of whole-cell patch-clamp

Ref ： T.K. Saito, 2002

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Introduction (cont.)

Ref ： T.K. Saito, 2002

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Operational procedure

Medium filling into the device

Clamping the cell

Exchange to the PS incl. medium

Excite PS with UV to perforate

membrane

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FabricationSOI

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Fabrication results

Upper structure

(Top view)

Upper structure

(Back view)

Lower structure

Implemented device

(Top view)

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Experiment setup

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Results

Micro channel in focus Cell in focus

Before BAT excitation After BAT excitation

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Results (cont.)

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Conclusion

Photodynamic perforation of cell membrane performed successfully.Use MEMS technology to improve the patch-clamp technique.The perforation method is expected to improve intracellular technologies.

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Thank you for your attention !