photosynthesis
DESCRIPTION
Photosynthesis. ‘Life is a tiny electric current kept moving by the sun’ Svent Gorki. Protocista. Algae. Plants. Photosynthesis Equation. carbon dioxide + water glucose + oxygen 6CO 2 + 6H 2 0 C 6 H 12 0 6. White Light. Electro magnetic spectrum. So Why Green?. - PowerPoint PPT PresentationTRANSCRIPT
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Photosynthesis
‘Life is a tiny electric current kept moving by the sun’
Svent Gorki
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Protocista
Algae Plants
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Photosynthesis Equation
carbon dioxide + water glucose + oxygen
6CO2 + 6H20 C6H1206
![Page 5: Photosynthesis](https://reader035.vdocuments.net/reader035/viewer/2022081604/56816618550346895dd96746/html5/thumbnails/5.jpg)
White Light
![Page 6: Photosynthesis](https://reader035.vdocuments.net/reader035/viewer/2022081604/56816618550346895dd96746/html5/thumbnails/6.jpg)
Electro magnetic spectrum
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So Why Green?
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Chloroplasts
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Chlorophyll
Chlorophyll plays an important role in photosynthesis as it
absorbs the energy form the sun
Fluorescence of extracted chlorophyll
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Absorption and action spectrum of chlorophyll
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Light IndependentStage
6 CO2
6H2OC6H12O6
24 H 6O2
Oxygen produced
12H2O
SUN
Chlorophyll
Water used
Lightdependent
Stage
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Measuring the rate of photosynthesis
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Extraction of chlorophyll and absorption spectrum
Assessment - Data Collection and Processing, Conclusion and Evaluation, manipulative skills
Extraction of chlorophyll
• Dip fresh leaf material into boiling water first to kill the cells and to make the cell membrane fully permeable to the solvent.
• Finely cut about 10g of leaf material (excluding the midrib and petiole) into a mortar and add a small amount of washed and dried sand.
• Grind up the leaf matter with 20cm3 of propanone, using a pestle, to make a concentrated leaf extract.
• Either filter the pigment solution or decant it (using a glass rod to hold back the debris), and place in a small glass centrifuge tube. Centrifuge the extract briefly, in order to clear all suspended organic matter.
• Pour the supernatant solution into a small glass tube, seal with a bung, label it, and stand this chlorophyll extract in the dark, an ice bath, until required.
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Measuring absorption of light by chlorophyll
• You will be shown how to use either a colorimeter or a
spectrophotometer to measure the absorption spectrum of your extract. Once you have done this you will need to plot a graph of absorption against wavelength for the data you collected.
• • Compare this to the absorption and action spectrums of chlorophyll.
How good is your match and how could the experiment be altered to improve your results.