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PHYSICAL METHODS FOR KILLING MICROBES • Sterilization methods – Incineration – Autoclave – Dry heat oven – Filter sterilization – Ultra violet light – Gamma radiation

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Page 1: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

PHYSICAL METHODS FOR KILLING MICROBES

• Sterilization methods– Incineration– Autoclave– Dry heat oven – Filter sterilization– Ultra violet light– Gamma radiation

Page 2: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

The direct flame represents a rapid sterilization method

Incineration

Page 3: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

The autoclave uses moist heat and pressure

Page 4: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

Boiling water involvesmoist heat

• This is not a reliable method for sterilization unless tyndallization is used.

• Why not?

Page 5: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

Pasteurization does not sterilize

• It is used to kill unwanted or harmful microbes

• Why doesn’t it sterilize?

Page 6: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

Hot-air (dry-heat) oven

Time and Temperature: 338F for 11/2 hours

Page 7: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

Filter Sterilization

Page 8: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

Filtration traps microorganisms

Page 9: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

Ultraviolet light can be used to control microbial growth

UV light attacks the cell’s DNA

What are some disadvantages in the use of UV for sterilization?

Page 10: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

The Ionizing and Electromagnetic Spectrum of Energies

Page 11: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

Thymine Dimers of DNA form when exposed to UV light

Page 12: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

Thymine Dimers

Page 13: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

CHECK MOLES FOR THESE CHANGES

Page 14: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

GAMMA RADIATION

A widely used method for sterilizing materials that would be adversely

altered by heat (plastics, lensed equip. heart-lung machines etc).

Page 15: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

Logo for gamma radiationLook for this logo on those foods that have been treated with gamma

radiation, such as meats, and produce.

Page 16: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

PRESERVATION METHODS

Drying (dehydration) Method

Salting Method

Low pH Method

Do these methods sterilize the food? Explain.

Page 17: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

General Principles ofChemical Control

Page 18: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

Resistance of microbes to chemical agents

Page 19: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

Disinfectants

Page 20: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

Killing Action of certain metals

Page 21: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation

Killing Action of Some Antiseptics


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