pierre jaccard1 chl-a fluorescence measurements 10.06.2013 - myocean tutorial, nersc, bergen
TRANSCRIPT
Pierre Jaccard 1
Chl-a Fluorescence Measurements
10.06.2013 - MyOcean Tutorial, NERSC, Bergen
Pierre Jaccard 210.06.2013 - MyOcean Tutorial, NERSC, Bergen
Differences from Physical Sensors
• Living species• Measurement principle differs• Chl-a Fluoresence is not Chl-a• Proxy• Neither really bad or good• Rather better or worse• Correct values from HPLC
K. Sørensen, M. Norli and A. Folkestad
3
Fluctuations in CHLAFL / CHLACONC
• Problem for using Chla_fl as phytoplankton biomass estimation
• Search for “solutions” in Ferrybox data series and other dataset/time-series from other investigations using data on:– Sun light– Day length– Temperature– Species composition– ...
Pierre Jaccard 4
Measurements In Practice
10.06.2013 - MyOcean Tutorial, NERSC, Bergen
HPLC(SPECTRO)
COEFFS
HPLC(SPECTRO)
Chl-a FluorescenceFluorometerChl-a
in situ
in vitro
COEFFSDelayed modeFluorometerChl-a
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Fluorescence high at small concentrations
Fluorescence low at high concentrations
Ferrybox Chl_a time-series 2002-2008
No yearly calibration of the fluorescence
Better match at high concentrations
With yearly calibration of the fluorescence
K. Sørensen, M. Norli and A. Folkestad
6
• Most months show the same trend:• High ratio at night• Lower ratio at daytime
FerryBox seasonal and night and day variation
Yearly calibration of the Chl-a fluorescense using all the Chl-a_hplc water samples
K. Sørensen, M. Norli and A. Folkestad
7
Variation of Chl-a_Fl/Chl-a for algal culture
1 2 3 4 5 6 7 8 9
0
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Nr. Algekulturer1 Chrysochromulina polylepis2 Dunaliella tertiolecta3 Emiliania huxleyi4 Oscilatoria agardii5 Prorocentrum minimum6 Prymnesium parvum7 Phaeodactylum tricornutum8 Selenastrum capricornutum9 Skeletonema costatum
Algal culture
Chl-
a_F
l/C
hl-
a
Determined during dark adapted algal from a exponetial growing algal culture
Factor 6 all species tested
Factor 2.5- 3 for Norw. waters
K. Sørensen, M. Norli and A. Folkestad
8
Validation of the chlorophyll-a fluorescence with monitoring data
K. Sørensen, M. Norli and A. Folkestad