plant variation data - what’s available, which new ... · plant variation data - what’s...
TRANSCRIPT
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Plant variation data - what’s available, whichnew projects are expected
Martin Mascher
IPK Gatersleben
4th transPLANT Workshop, Hinxton, UKJuly 1st, 2015
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IPK Gatersleben
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Ex situ genebanks around the world
I Conserve, characterize and utilize plant genetic variation
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Barley diversity at IPK Gatersleben
slide 4
Photos by Michael Grau, IPK genebank
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Types of variation
I Phenotypic variation
• ”Visible” traits: plant height,flowering time, pathogen resistance,. . .
• Molecular phenotypes: metabolites,hormone, ionome, . . .
• Ecological data: geographic origin,environmental parameters, . . .
I Genetic variation
SNPs, Indels, structural variation,karyotypes, ploidy, genome size
I Inter-specific variation
Phylogeny, comparative genomics
slide 5
http://nativetraits.blogspot.de
![Page 6: Plant variation data - what’s available, which new ... · Plant variation data - what’s available, which ... . Genotyping strategies ... I Mission statement:](https://reader031.vdocuments.net/reader031/viewer/2022022508/5aced6137f8b9a8b1e8bf28d/html5/thumbnails/6.jpg)
Types of variation
I Phenotypic variation
• ”Visible” traits: plant height,flowering time, pathogen resistance,. . .
• Molecular phenotypes: metabolites,hormone, ionome, . . .
• Ecological data: geographic origin,environmental parameters, . . .
I Genetic variation
SNPs, Indels, structural variation,karyotypes, ploidy, genome size
I Inter-specific variation
Phylogeny, comparative genomics
slide 5
http://nativetraits.blogspot.de
Badaeva et al., PLOS ONE, 2015
![Page 7: Plant variation data - what’s available, which new ... · Plant variation data - what’s available, which ... . Genotyping strategies ... I Mission statement:](https://reader031.vdocuments.net/reader031/viewer/2022022508/5aced6137f8b9a8b1e8bf28d/html5/thumbnails/7.jpg)
Types of variation
I Phenotypic variation
• ”Visible” traits: plant height,flowering time, pathogen resistance,. . .
• Molecular phenotypes: metabolites,hormone, ionome, . . .
• Ecological data: geographic origin,environmental parameters, . . .
I Genetic variation
SNPs, Indels, structural variation,karyotypes, ploidy, genome size
I Inter-specific variation
Phylogeny, comparative genomics
slide 5
http://nativetraits.blogspot.de
Badaeva et al., PLOS ONE, 2015
![Page 8: Plant variation data - what’s available, which new ... · Plant variation data - what’s available, which ... . Genotyping strategies ... I Mission statement:](https://reader031.vdocuments.net/reader031/viewer/2022022508/5aced6137f8b9a8b1e8bf28d/html5/thumbnails/8.jpg)
Applications of variant data
I Population genetics
Nucleotide diversity, demographicinference, targets of selection
I Assocation genetics
Linking phenotype to genotype,GWAS
I Conservation management in situ andex situ
Molecular passport data
I (Pre)-breeding
Management of breeding programs,characterization of exotic donors
slide 6
Lyu et al., Nat. Comm., 2013
![Page 9: Plant variation data - what’s available, which new ... · Plant variation data - what’s available, which ... . Genotyping strategies ... I Mission statement:](https://reader031.vdocuments.net/reader031/viewer/2022022508/5aced6137f8b9a8b1e8bf28d/html5/thumbnails/9.jpg)
Applications of variant data
I Population genetics
Nucleotide diversity, demographicinference, targets of selection
I Assocation genetics
Linking phenotype to genotype,GWAS
I Conservation management in situ andex situ
Molecular passport data
I (Pre)-breeding
Management of breeding programs,characterization of exotic donors
slide 6
Lyu et al., Nat. Comm., 2013
Romay et al., Genome Biology, 2013
![Page 10: Plant variation data - what’s available, which new ... · Plant variation data - what’s available, which ... . Genotyping strategies ... I Mission statement:](https://reader031.vdocuments.net/reader031/viewer/2022022508/5aced6137f8b9a8b1e8bf28d/html5/thumbnails/10.jpg)
Applications of variant data
I Population genetics
Nucleotide diversity, demographicinference, targets of selection
I Assocation genetics
Linking phenotype to genotype,GWAS
I Conservation management in situ andex situ
Molecular passport data
I (Pre)-breeding
Management of breeding programs,characterization of exotic donors
slide 6
Lyu et al., Nat. Comm., 2013
Romay et al., Genome Biology, 2013
![Page 11: Plant variation data - what’s available, which new ... · Plant variation data - what’s available, which ... . Genotyping strategies ... I Mission statement:](https://reader031.vdocuments.net/reader031/viewer/2022022508/5aced6137f8b9a8b1e8bf28d/html5/thumbnails/11.jpg)
Applications of variant data
I Population genetics
Nucleotide diversity, demographicinference, targets of selection
I Assocation genetics
Linking phenotype to genotype,GWAS
I Conservation management in situ andex situ
Molecular passport data
I (Pre)-breeding
Management of breeding programs,characterization of exotic donors
slide 6
Lyu et al., Nat. Comm., 2013
Romay et al., Genome Biology, 2013
![Page 12: Plant variation data - what’s available, which new ... · Plant variation data - what’s available, which ... . Genotyping strategies ... I Mission statement:](https://reader031.vdocuments.net/reader031/viewer/2022022508/5aced6137f8b9a8b1e8bf28d/html5/thumbnails/12.jpg)
Genotyping strategies
I SNP arrays
Genotyping of known variants,ascertainment bias
I Whole-genome shotgun
Most comprehensive, most expensive
I Genotyping-by-sequencing, RADseq
Cheap, tens of thousands of markers
I RNAseq / Exome capture
Comprehensive discovery andtyping of variants in genes
slide 7
http://www.ibbl.lu
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Genotyping strategies
I SNP arrays
Genotyping of known variants,ascertainment bias
I Whole-genome shotgun
Most comprehensive, most expensive
I Genotyping-by-sequencing, RADseq
Cheap, tens of thousands of markers
I RNAseq / Exome capture
Comprehensive discovery andtyping of variants in genes
slide 7
http://www.ibbl.lu
http://www.illumina.com
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Genotyping strategies
I SNP arrays
Genotyping of known variants,ascertainment bias
I Whole-genome shotgun
Most comprehensive, most expensive
I Genotyping-by-sequencing, RADseq
Cheap, tens of thousands of markers
I RNAseq / Exome capture
Comprehensive discovery andtyping of variants in genes
slide 7
http://www.ibbl.lu
http://www.illumina.com
![Page 15: Plant variation data - what’s available, which new ... · Plant variation data - what’s available, which ... . Genotyping strategies ... I Mission statement:](https://reader031.vdocuments.net/reader031/viewer/2022022508/5aced6137f8b9a8b1e8bf28d/html5/thumbnails/15.jpg)
Genotyping strategies
I SNP arrays
Genotyping of known variants,ascertainment bias
I Whole-genome shotgun
Most comprehensive, most expensive
I Genotyping-by-sequencing, RADseq
Cheap, tens of thousands of markers
I RNAseq / Exome capture
Comprehensive discovery andtyping of variants in genes
slide 7
http://www.ibbl.lu
http://www.illumina.com
![Page 16: Plant variation data - what’s available, which new ... · Plant variation data - what’s available, which ... . Genotyping strategies ... I Mission statement:](https://reader031.vdocuments.net/reader031/viewer/2022022508/5aced6137f8b9a8b1e8bf28d/html5/thumbnails/16.jpg)
Plant resequencing projects
Species # acc strategy publication
Rice 3000 WGS 3000 RGP, 2014, GigaScience
Maize 2815 GBS Romay et al., 2013, Genome Biol
Rice 1529 WGS Huang et al., 2012, Nature
A. thaliana 1135 WGS Cao et al., 2011, Nat Genet [and others]
Sorghum 971 GBS Morris et al., 2013, PNAS
P. trichocarpa 544 WGS Evans et al., 2014, Nat Genet
Maize 503 RNAseq Hirsch et al., 2014, Plant Cell
Soybean 302 WGS Zhou et al., 2005, Nat Biotech
Barley 270 EC, GBS unpublished
Maize 113 WGS Chia et al., 2012, Nat Genet
Wheat 62 EC, GBS Jordan et al., 2015, Genome Biol
Mimulus spp. 19 WGS Brandvain et al., 2014, PLOS Genet
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Plant resequencing projects
Species # acc strategy publication
Rice 3000 WGS 3000 RGP, 2014, GigaScience
Maize 2815 GBS Romay et al., 2013, Genome Biol
Rice 1529 WGS Huang et al., 2012, Nature
A. thaliana 1135 WGS Cao et al., 2011, Nat Genet [and others]
Sorghum 971 GBS Morris et al., 2013, PNAS
P. trichocarpa 544 WGS Evans et al., 2014, Nat Genet
Maize 503 RNAseq Hirsch et al., 2014, Plant Cell
Soybean 302 WGS Zhou et al., 2005, Nat Biotech
Barley 270 EC, GBS unpublished
Maize 113 WGS Chia et al., 2012, Nat Genet
Wheat 62 EC, GBS Jordan et al., 2015, Genome Biol
Mimulus spp. 19 WGS Brandvain et al., 2014, PLOS Genet
slide 9
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Plant resequencing projects
Species # acc strategy publication
Rice 3000 WGS 3000 RGP, 2014, GigaScience
Maize 2815 GBS Romay et al., 2013, Genome Biol
Rice 1529 WGS Huang et al., 2012, Nature
A. thaliana 1135 WGS Cao et al., 2011, Nat Genet [and others]
Sorghum 971 GBS Morris et al., 2013, PNAS
P. trichocarpa 544 WGS Evans et al., 2014, Nat Genet
Maize 503 RNAseq Hirsch et al., 2014, Plant Cell
Soybean 302 WGS Zhou et al., 2005, Nat Biotech
Barley 270 EC, GBS unpublished
Maize 113 WGS Chia et al., 2012, Nat Genet
Wheat 62 EC, GBS Jordan et al., 2015, Genome Biol
Mimulus spp. 19 WGS Brandvain et al., 2014, PLOS Genet
slide 10
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Plant resequencing projects
Species # acc strategy publication
Rice 3000 WGS 3000 RGP, 2014, GigaScience
Maize 2815 GBS Romay et al., 2013, Genome Biol
Rice 1529 WGS Huang et al., 2012, Nature
A. thaliana 1135 WGS Cao et al., 2011, Nat Genet [and others]
Sorghum 971 GBS Morris et al., 2013, PNAS
P. trichocarpa 544 WGS Evans et al., 2014, Nat Genet
Maize 503 RNAseq Hirsch et al., 2014, Plant Cell
Soybean 302 WGS Zhou et al., 2005, Nat Biotech
Barley 270 EC, GBS unpublished
Maize 113 WGS Chia et al., 2012, Nat Genet
Wheat 62 EC, GBS Jordan et al., 2015, Genome Biol
Mimulus spp. 19 WGS Brandvain et al., 2014, PLOS Genet
slide 10
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Plant resequencing projects
Species # acc strategy publication
Rice 3000 WGS 3000 RGP, 2014, GigaScience
Maize 2815 GBS Romay et al., 2013, Genome Biol
Rice 1529 WGS Huang et al., 2012, Nature
A. thaliana 1135 WGS Cao et al., 2011, Nat Genet [and others]
Sorghum 971 GBS Morris et al., 2013, PNAS
P. trichocarpa 544 WGS Evans et al., 2014, Nat Genet
Maize 503 RNAseq Hirsch et al., 2014, Plant Cell
Soybean 302 WGS Zhou et al., 2005, Nat Biotech
Barley 270 EC, GBS unpublished
Maize 113 WGS Chia et al., 2012, Nat Genet
Wheat 62 EC, GBS Jordan et al., 2015, Genome Biol
Mimulus spp. 19 WGS Brandvain et al., 2014, PLOS Genet
slide 11
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Plant resequencing projects
Species # acc strategy publication
Rice 3000 WGS 3000 RGP, 2014, GigaScience
Maize 2815 GBS Romay et al., 2013, Genome Biol
Rice 1529 WGS Huang et al., 2012, Nature
A. thaliana 1135 WGS Cao et al., 2011, Nat Genet [and others]
Sorghum 971 GBS Morris et al., 2013, PNAS
P. trichocarpa 544 WGS Evans et al., 2014, Nat Genet
Maize 503 RNAseq Hirsch et al., 2014, Plant Cell
Soybean 302 WGS Zhou et al., 2005, Nat Biotech
Barley 270 EC, GBS unpublished
Maize 113 WGS Chia et al., 2012, Nat Genet
Wheat 62 EC, GBS Jordan et al., 2015, Genome Biol
Mimulus spp. 19 WGS Brandvain et al., 2014, PLOS Genet
slide 12
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Plant resequencing projects
Species # acc strategy publication
Rice 3000 WGS 3000 RGP, 2014, GigaScience
Maize 2815 GBS Romay et al., 2013, Genome Biol
Rice 1529 WGS Huang et al., 2012, Nature
A. thaliana 1135 WGS Cao et al., 2011, Nat Genet [and others]
Sorghum 971 GBS Morris et al., 2013, PNAS
P. trichocarpa 544 WGS Evans et al., 2014, Nat Genet
Maize 503 RNAseq Hirsch et al., 2014, Plant Cell
Soybean 302 WGS Zhou et al., 2005, Nat Biotech
Barley 270 EC, GBS unpublished
Maize 113 WGS Chia et al., 2012, Nat Genet
Wheat 62 EC, GBS Jordan et al., 2015, Genome Biol
Mimulus spp. 19 WGS Brandvain et al., 2014, PLOS Genet
slide 13
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BRIDGE: genotyping the IPK barley collection
slide 14
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BRIDGE: biodiversity informatics
slide 15
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New information infrastructures for genotypic datasets
slide 16
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What about the phenotypes?
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What about the phenotypes?
slide 17
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DivSeek: Harnessing crop diversity to feed the future
I Who is DivSeek?
Currently 58 partners worldwide:Gene banks, breeders,plant scientists, database andcomputational experts
I Aim:
Understanding and utilizing genetic variation in plantimprovement
I Mission statement:
”Characterize crop diversity and develop a unified, coordinatedand cohesive information management platform to provideeasy access to genotypic and phenotypic data associated withgenebank germplasm.”
http://www.divseek.org
slide 18
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DivSeek: Harnessing crop diversity to feed the future
I Who is DivSeek?
Currently 58 partners worldwide:Gene banks, breeders,plant scientists, database andcomputational experts
I Aim:
Understanding and utilizing genetic variation in plantimprovement
I Mission statement:
”Characterize crop diversity and develop a unified, coordinatedand cohesive information management platform to provideeasy access to genotypic and phenotypic data associated withgenebank germplasm.”
http://www.divseek.org
slide 18
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DivSeek: Harnessing crop diversity to feed the future
I Who is DivSeek?
Currently 58 partners worldwide:Gene banks, breeders,plant scientists, database andcomputational experts
I Aim:
Understanding and utilizing genetic variation in plantimprovement
I Mission statement:
”Characterize crop diversity and develop a unified, coordinatedand cohesive information management platform to provideeasy access to genotypic and phenotypic data associated withgenebank germplasm.”
http://www.divseek.org
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Acknowledgments
I Andreas Graner
I Nils Stein
I Uwe Scholz
I Andreas Borner
I Helmut Knupffer
I Michael Grau
I Robbie Waugh
I Joanne Russell
I Ian Dawson
I Gary Muehlbauer
slide 19