Plant-virus interactionsPlant-virus interactionsINRA Bordeaux-AquitaineINRA Bordeaux-Aquitaine

IBVM, IBVM, INRA Bordeaux-AquitaineINRA Bordeaux-Aquitaine

History of the groupHistory of the group

• Fruit tree viruses: started in the 60’sFruit tree viruses: started in the 60’s

• In the 90’s: 3 focusesIn the 90’s: 3 focuses

– Virus identification, detection and populations

– Virus resistance using transgenic plants

– Natural virus resistance: host-virus interactions

• This last item was re-focusedThis last item was re-focused

– Nepoviruses decreased

– Lettuce mosaic virus started and took over in 1999

Plant-potyvirus interactionsPlant-potyvirus interactions

• Identify virus determinantsIdentify virus determinants– Necrosis in the lettuce cultivar « Trocadéro »: HcPro

– Ability to overcome mo1: central region of LMV genome

• mo1 mo1 encodes eIF4Eencodes eIF4E– 2 alleles in 2 types of genotypes in crop lettuce

– 1 more haplotype in crop lettuce

– 1 more haplotype in weed lettuce

• How does it work?How does it work?– Quality / structural aspects of the interactions

• Yeast two-hybrid, ELISA, spectrofluorescence

– Other partners?

• LMV HcPro inhibits the proteasome’s RNaseLMV HcPro inhibits the proteasome’s RNase

Plant-potyvirus interactions: geneticsPlant-potyvirus interactions: genetics

• Genetic approach implemented in 2 hosts:Genetic approach implemented in 2 hosts:– Apricot

– Arabidopsis

• In Arabidopsis:In Arabidopsis:– Screen ecotype collections with virus collections (PPV, LMV)

– Identify many sources of resistance• Local – Systemic – Symptoms• Monogenic – Oligogenic• Dominant – Recessive

– Begin to clone some of these resistance sources:• rlm1• rpv1

Next needs: genetic ressources to go back to Next needs: genetic ressources to go back to crop speciescrop species