playing cards for memory and total recall pages 2 to 4: keyword-cards pages 5 to 7: keyword-cards...
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Playing cards for Memory and Total recall
Pages 2 to 4: keyword-cards
Pages 5 to 7: keyword-cards with corresponding explanation-cards
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Restriction Restriction Star fragment endonuclease activity Isoschizo- Gel
mer electrophoresis Slot
Growth Ethidium Electrophoretic
rate bromide mobility
Double Origin of Selectable digestion replication marker
pBR 322 MCS Polylinker
Complex -Galacto- High copy media sidase plasmid
Low copy Defined DNA plasmid media ligases Competent Escherichia pUC 19 cells coli Electro- Ligation OD poration
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EcoR I lac-Operon Plasmid
Cloning Electropho- Base vector resis pair
Antibiotic Blunt end Sticky end resistance enzymes enzymes Restriction Endo- map nucleases Palindrome
DNA Recognition Target band site sequence Restriction Compatible enzyme Ampicillin ends
Electropho- Restriction Salt resis marker buffer competent lacZ gene ORI Heat shock Bromo- Xylen- Type I phenol blue xyanol enzyme
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Hybridi- Probe Label sation Denatura- Southern RBS tion Blot Target Promoter Terminator sequence Expression Southern Western vector Blot Blot Agarose PCR Primer
Annealing Synthesis Cycle
Cassette
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DNA modifying enzymes that recognise specific motifs of double stranded DNA and cleave it into fragments of different lengths
type II restriction enzyme: recognises a specific palindrome sequence motif (GAATTC)
sequence motif with an axis of rotational symmetry, the sequence in 5`3`-direction is equal on the both strands
small rings of double stranded extrachromosomal and independently reproduced DNA, natural types are important for antibiotic resistance and sexual reproduction of bacteria
vehicle of genetic engineering
shuttle that allows reintroducing of foreign DNA into a host organism
Endonucleases
Palindrome
Plasmids
EcoRI
Vector
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bacterial plasmids with features for reintroducing of small foreign DNA-fragments into host organisms with the purpose of producing significant amounts of recombinant DNA
Cloning vector
diagram showing sites of cleavage of a DNA molecule, for example of a plasmid vector
Restriction map
Cleavage of DNA with this type of endonucleases creates a short complementary single stranded overhang in each cleaved end
Sticky end enzymes
Blunt end enzymes
Endonucleases that form no 5’or 3’ overhangs after cleavage
Competent cellsBacterial cells which are able to incorporate foreign DNA
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lac-OperonBacterial expression unit of genes for lactose utilisation
Agarose gel electrophoresis
Method that separates DNA fragments in an electric field as a function of their lengths
PolylinkerStaged recognition sites of different restriction enzymes, a feature of vectors for variable insertion of foreign DNA
IsoschizomerRestriction enzymes that recognise the same target sequence
Ethidium bromide (EtBr)
Stain that intercalates into double-stranded DNA. Under UV-light the complex of DNA and the stain fluoresces pink