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Introduction: Up to now there are not studies on comparative response to cardiopul-monary exercise testing (CPET) during transient left bundle branch block (LBBB) andnormal intra-ventricular conduction (NIVC) in female athletes.Objectives: The aim of this study is to fill the gap existing to date for comparative studieson the response to CPET during transient LBBB and NIVC in female athletes.Methods: From January 2004 to April 2013 two cases of transient LBBB were detected on10107 (0.02%) consecutive female athletes referred for cardiac preventive screening tocompetitive sports activity,. According to protocol both athletes, respectively a 25 years oldroller skater and a 27 years old water-polo player, performed a CPET, and were invited to comeback every sixmonths for control.Havebeenmade twenty-sixCPET, respectively twelveduringLBBB and fourteen during NIVC. The rest and peak oxygen uptake (VO2), oxygen pulse (O2pulse), heart rate (HR), blood pressure, and the peak work load (PWL) sustained, measuredduring CPET performed in LBBB and NIVC were compared using the primer software.Results: The CPET data collected during LBBB and normal conduction expressed as meanplus/minus standard deviation (SD), and the P value were shown on the table. During thefollow up the LBBB spontaneously disappear after fifty-four months in both females.

Table 1. CPET results during LBBB vs NIVC

CPET measure LBBBvsNIVC atRest Pvalue LBBBvsNIVCatPeak ofexercise Pvalue

Heart Rate 73.9+/-8.6 vs 73.1+/-7.9 P¼0.820 176.7+/-4.02 vs 176.3+/-3.7 P¼0.798

SBP 128.1+/-2.3 vs 128.2 +/-2.3 P¼0.915 182.2+/-2.3 vs 221.7+/-2.3 P¼0.000

DBP 83.18+/-2.3 vs 81.07 +/-1.9 P¼0.019 102.7+/-4.2 vs 91.4+/-3.3 P¼0.000

VO2 ml/Kg/min 8.3+/-1.9 vs 8.2+/-1.8 P¼0.863 50.3+/-9.06 vs 50.1+/-8.05 P¼0.954

O2 pulse ml/beat 6.01+/-1.3 vs 5.87+/-1.4 P¼0.802 13.06+/-2.3 vs 12.8+/-2.4 P¼0.790

PWL 166.8+/-2.3 vs 167.1+/-2.3 P¼0.749

Conclusion: This study is the first to compare the CPET response in female athletes withtransient LBBB during the transitory conduction disturbance and NIVC. The above resultssupport the notion that except the minor increase in peak SBP, the peak HR as well as theoverall functional capacity expressed as peak VO2 achieved and PWL sustained in NIVCremain unchanged during LBBB.Disclosure of Interest: None Declared

PM023

Aerobic Exercise Altered Electrocardiogram And Cardiac Biomarkers In CachecticTumor-Bearing Rats

Aline Toneto1, Luiz Alberto F. Ramos*1, Emiliane Salomão1, Miguel A. Areas1,Maria Cristina C. Gomes-Marcondes11Biology Structural and Physiology, STATE UNIVERSITY OF CAMPINAS - UNICAMP,Campinas, Brazil

Introduction: Cancer-cachexia state promotes intense involuntary weight loss, wastingskeletal muscle protein due to higher proteolysis and/or decreased protein synthesis.Physical exercise can delay the onset of anorexia and cachexia, inhibiting tumor growth andmaintain the body sources associated to less fatigue and skeletal muscle mass, being analternative of co-adjuvant cancer treatment1.Objectives: This work evaluated exercise effects on electrocardiogram (ECG) and cardiacbiomarkers in the cachectic rats bearing Walker-256 tumor (W) subjected to mild aerobicexercise.Methods: Adult rats were distributed into 4 groups: C–control rats; W- tumor-bearing;EC–exercised rats (swimming for 9 weeks) and EW–exercised tumor-bearing rats (5 ani-mals per group). The electrocardiogram was evaluated after anesthesia (ketamine 100mg/Kg. plus xelazine 7mg/Kg intramuscular) during pre-agonic state assayed by LabChartsoftware. Cardiac biomarkers (MPO, TIMP, PAI-total) were evaluated in heart musclehomogenate analysed by multiplex assay (Millipore-Luminex). Difference among groupsassayed using one-way ANOVA followed by Bonferroni’s test (P<0.05).Results: ECG parameters: Heart rate decrease in W and WE groups. QT-c interval tendedtoincreased only in W rats (P¼0.109); recovering in EW. T wave amplitude increased inEW group.Cardiac biomarkers: MPO and TIMP increasedespecially in W group; PAI-totalincreased in both tumor-bearing groups, especially in EW rats.

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Conclusion: Aerobic exercise has benefic effects in Walker tumor-bearing rats partlyrestoring the ventricular function (similar QT-c), but did not prevent the increased cardiacbiomarkers related to thrombogenesis processes.Reference

1. SALOMÃO et al, Nutrition and Cancer, 2010, 62(8), 1095.

Disclosure of Interest: None Declared

PM024

The Common Genotypes of Long Qt Syndrome And The Role Of ElectrocardiogramPrediction—The China Experience

Cuilan Li1, Yuanfeng Gao2, Wenling Liu*2, Li Zhang3, Dayi Hu2, and For ChineseChannelopathy Register Investigators1Heart Center, 2Peking University People’s Hospital, Beijing, China, 3Lankenau Medical Center,Lankenau Institute for Medical Research, Jefferson Medical College, Philadelphia, United States

Introduction: LQTS is an inherited cardiac disorder characterized by QT prolongation andincreased risks of sudden death. Mutations in 13 genes have been identified in hereditaryLQTS. However, only 60% to 70% of the individuals affected with LQTS could be detectedto have mutations in these genes. Approximately 90% of known mutations were onLQTS1-3 causing genes.Objectives: Since the vast majority of mutation carriers of LQT1-3 presented with geno-type-specific ECG patterns, in this study we tested its utility in targeted genotyping in alarge cohort of Chinese patients with LQTS.Methods: The ECG-pattern-guided genotyping was conducted in 74.3% (107/144) pro-bands with LQTS enrolled in the Chinese Channelopathy Register Study. Probands withatypical ECG patterns were excluded from this study (n¼37). The initial 12-lead ECG ofeach patient was evaluated. Individuals were considered affected if they have a prolongedQT interval (QTc > 450ms in male and > 470 ms in females). Patients were predicted aspossible LQT1, 2 or 3 based on the presence of ECG patterns typical to each genotype.Based on the ECG predictions, mutational screening were performed using PCR and directDNA sequence analysis. DNA samples from 50 healthy Han Chinese served as controls.Results: A total of 67 mutations, including 29 novel mutations, were identified. Of which 26were KCNQ1, 39 KCNH2 and 2 SCN5Amutations, respectively. Among the 107 patients, theECG-diagnosis results showed that there were 38 LQT1, 65 LQT2 and 4 LQT3, while genescreening results showed that 26, 48 and 2 patients could be diagnosed genetically as LQT1,LQT2 and LQT3, respectively. The mutational positive rate was 71.0% (76/107) for allpatients. To be specific, the ECG predicted genotype matched the mutation results in 68.4%(26/38), 73.8% (48/65) and 50.0% (2/4) for LQT1, LQT2 and LQT3, respectively.Conclusion: This study shows thatmost mutations harbored by LQTS patients are on LQT1-3 causing genes while LQT2 the most common in Chinese. ECG prediction-guidedgenotyping is proven time and economically efficient, except for a low predictable rate of ECG-diagnosis in LQT3. Our experience sheds lights in LQTS studies in developing countries.Disclosure of Interest: None Declared

PM025

A Severe Type of Long QT Syndrome and Cardiovascular Anomaly in a HomozygousCarrier of a Novel KCNH2 Mutation and Findings in Family Screening

Khalfan S. Al-Senaidi*1, Guoliang Wang2, Li Zhang3, Dominik A. Beer4,Abdullah M. Al-Farqani5, Salim N. Al-Maskaryi5, Daniel J. Penny2, Peter R. Kowey3,Yuxin Fan21Cardiology, Sultan Qaboos Univ. Hospital, Muscat, Oman, 2Section of Cardiology, Departmentof Pediatrics, John Welsh Cardiovascular Diagnostic Laboratory, Baylor College of Medicine,Houston, 3Cardiology, Lankenau Medical Center, Lankenau Institute for Medical Research,Jefferson Medical College, 4Philadelphia College of Osteopathic Medicine, Philadelphia, UnitedStates, 5Cardiology, Royal Hospital, Muscat, Oman

Introduction: Patients with inherited long QT syndrome (LQTS), especially if carryinghomozygous mutations, are prone to torsade de pointes (TdP) and sudden death (SD).Identifying individuals with LQTS is of particular importance for SD prevention.

GHEART Vol 9/1S/2014 j March, 2014 j POSTER/2014 WCC Posters