SDHAPolyclonal ANTIBODYCatalog Number:14865-1-AP 13 Publications

For Research Use Only

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Basic Information Catalog Number:14865-1-APSize:270 μg/mlSource:RabbitIsotype:IgGPurif ication Method:Antigen aff inity purif icationImmunogen Catalog Number:AG6677

GenBank Accession Number:BC001380GeneID (NCBI):6389Full Name:succinate dehydrogenase complex, subunit A,f lavoprotein (Fp)Calculated MW:73 kDaObserved MW:62-73 kDa

Recommended Dilutions:WB 1:500-1:1000 IP 0.5-4.0 ug for IP and 1:500-1:1000 for WBIHC 1:100-1:400 IF 1:50-1:500

Applications Tested Applications:IF, IHC, IP, WB, ELISACited Applications:WBSpecies Specif icity:human,mouse,ratCited Species:human, mouse, rat

Positive Controls:

WB : HEK-293 cells; HeLa cells, HepG2 cells,mouse brain tissue

IP : mouse brain tissue;

IHC : human liver cancer tissue; human hearttissue, human kidney tissue

IF : HepG2 cells; mouse liver tissue, mouse hearttissue, mouse brain tissue

Background Information SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the f lavoproteinsubunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferringelectrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) canbe removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.

Notable Publications Author Pubmed ID Journal Application

Jing Zhang 26492917 EMBO J WB

Xiaoyu Ma 25301052 Nat Commun WB

Yingyan Han 27239088 Dis Markers WB

Storage Storage:Store at -20ºC. Stable for one year af ter shipment.Storage Buffer:PBS with 0.1% sodium azide and 50% glycerol pH 7.3.Aliquoting is unnecessary for -20ºC storage

Selected Validation Data

HEK-293 cells were subjected to SDS PAGEfollowed by western blot with 14865-1-AP(SDHAantibody) at dilution of 1:800 incubated at roomtemperature for 1.5 hours

Immunofluorescent analysis of ( 4% PFA) f ixedHepG2 cells using 14865-1-AP (SDHA antibody)at dilution of 1:50 and Alexa Fluor 488-conjugatedAff iniPure Goat Anti-Rabbit IgG(H+L)

IP Result of anti-SDHA (IP:14865-1-AP, 4ug;Detection:14865-1-AP 1:500) with mouse braintissue lysate 4000ug.

Immunohistochemistry of paraff in-embeddedhuman liver cancer tissue slide using 14865-1-AP( SDHA Antibody) at dilution of 1:200 (under10x lens). heat mediated antigen retrieved withTris-EDTA buffer(pH9).

Immunohistochemistry of paraff in-embeddedhuman liver cancer tissue slide using 14865-1-AP( SDHA Antibody) at dilution of 1:200 (under40x lens). heat mediated antigen retrieved withTris-EDTA buffer(pH9).