poster-next generation self-healing concrete-infusing bacteria into engineered cementitious...
TRANSCRIPT
Purpose & Objectives
ROL ♜ S p o r o s a r c i n a p a s t e u r i i
♜ Cult ivat ion v 3 0 ° C S h a k i n g t a b l e v 2 4 h v à petr i d ish
♜ R e p e a t e d i n v a t
♜ S o l i d m e d i a v Y e a s t : 2 . 0 0 g v Ammonium sul fate ���
(NH4) 2 SO 4 : 1 .00 g v Tr is ���
(HOCH 2 ) 3 (CNH 2 ) : ���1 . 5 7 g
v Agar : 2 .00 g
♜ Liquid media v Y e a s t : 2 0 . 0 0 g i n 4 0 0
m L v Ammonium sul fate : ���
1 0 . 0 0 g i n 3 0 0 m L v 5 . 7 3 g o f t r i s in ���
3 0 0 m L
♜ Derived f rom ECC R0 ���(L i , 2004)
♜ Changes to Formula: v Less superplast ic izer v W/C = 0 .395
♜ Amounts v Cement : 446.39 g
♜ F 1 1 0 s i l i c a s a n d :4 4 6 . 0 5 g
♜ Polyvinyl a lcohol (PVA) f ibers : 11 .90 g
♜ Water/medium:1 7 8 . 9 0 g ( f o r E C C : 1 . 6 g o f superplast ic izer )
Bacteria Culturing
Nutrient Mediums
Mix
Cubes: 5.08 x ���5.08 x ���5.08 cm ���
Beams: 12.7 x ���2.54 x 2.54 cm ���
(Note that each beam was in i t ia l ly cast in a
3 0 . 4 c m m o l d s b u t w a s b i s e c t e d a f t e r o n e w e e k )
1: Laboratory v Control v Lime water v 3 0 ° C
3: Underground v Buried 21.6 cm
2: Exposed v Dry, l i t t le ra in (Angel , 2013)
0% 2% 4% 6% 8% 10% 12% 14%
Control: ECC
Control: ECC + Medium
Control: ECC + Bacteria
Exposed: ECC
Exposed: ECC + Medium
Exposed: ECC + Bacteria
Undergound: ECC
Undergound: ECC + Medium
Undergound: ECC + Bacteria
R.F. % Increase
Envi
ron
men
tal G
rou
p: T
ype
Self-Healing in all Groups
-‐5.00%
0.00%
5.00%
10.00%
15.00%
20.00%
0.00% 5.00% 10.00% 15.00% 20.00% 25.00% Perc
ent
incr
ease
in R
.F. a
fter
rec
ove
ry
Percent reduction in R.F. from damage
Damage Healing Correla.on
Laboratory Environment Underground Environment
Exposed Environment Linear (Laboratory Environment)
Linear (Underground Environment) Linear (Exposed Environment)
0%
2%
4%
6%
8%
10%
12%
ECC ECC+ Medium ECC + Bacteria
R.F
. % in
crea
se
Type
Healing amongst ECC Types
0%
2%
4%
6%
8%
10%
12%
14%
Control Exposed Underground
R.F
% in
crea
se
Environment
Healing by Environment
0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80
OPC
-‐R
OPC
-‐M
OPC
-‐B
ECC-‐R
ECC-‐M
ECC-‐B
Exponential Rate of Water
Uptake
0
10000
20000
30000
40000
50000
60000
70000
Pasc
als
req
uir
ed fo
r Fa
ilu
re
Individual ECC specimen ECC type average
Compressive Strength
0.00%
2.00%
4.00%
6.00%
8.00%
10.00%
12.00%
14.00%
0 10 20 30 40 50 60
Mas
s u
pta
ke b
y %
Time [hrs]
Sorptivity
OPC-‐R
OPC-‐M
OPC-‐B
ECC-‐R
ECC-‐M
ECC-‐B
Natural Self-Healing
Bacterial Concrete
ECC
ECC in the Field
Specimen Preparation
Environments
RF Testing
Comp. & Sorpt. Testing + Stats
General Trends
Type Healing
Field Healing
Comp. & Sorpt. Results
Hypothesis My Role Conclusions
Methods Results
I would l ike to thank my mentor , Dr . Paramita M o n d a l , he r do cto r al cand idat es , Pet e S t y n o s k i and Bin Zh an g al ong with gra duate s t udent J e e v a k a S o m a r a t n a, my sc ience research advisors : Mr . David Keith , Mr . Ken Kaplan, and Ms. Stephanie Greenwald, my parents : Dr . Howard Kap lan an d M s. J e nni fer L acks Kapl a n, my s te p m other : J anet S h i m e r, and last l y m y grandfather : Dr . Sanford Lac k s .
Acknowledgements