Please refer disclaimer Overleaf.

Potato Dextrose Agar w/Chloramphenicol M1941

Composition**Ingredients Gms / LitrePotatoes, infusion from 200.000Dextrose (Glucose) 20.000Agar 15.000Chloramphenicol 0.050Final pH ( at 25°C) 5.6±0.2**Formula adjusted, standardized to suit performance parameters

DirectionsSuspend 39.05 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Mix well before dispensing. In specific work, when pH 3.5 is required, acidify the medium with sterile 10% tartaric acid. The amount of acid required for 100 ml. of sterile, cooled medium is approximately 1 ml. Do not heat the medium after addition of the acid.

Principle And InterpretationPotato Dextrose Agar is recommended by APHA (7) and FDA (2) for plate counts of yeasts and moulds in the examination of foods and dairy products (8). Potato Dextrose Agar is also used for stimulating sporulation, for maintaining stock cultures of certain dermatophytes and for differentiation of typical varieties of dermatophytes on the basis of pigment production (6). Potato Dextrose Agar with chloramphenicol is recommended for the selective isolation of fungi.Potato infusion and dextrose promote luxuriant fungal growth. Adjusting the pH of the medium by tartaric acid to 3.5, inhibits the bacterial growth. Heating the medium after acidification should be avoided as it may hydrolyse the agar which can render the agar unable to solidify. Chloramphenicol inhibits a wide range of Gram-positive and Gram-negative bacteria which makes the medium selective for fungi (5).

Intended Use:Recommended for the selective isolation and enumeration of yeasts and moulds from dairy and other food products.

Type of specimen Food and dairy samples

For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (1,7,8). After use, contaminated materials must be sterilized by autoclaving before discarding.

Specimen Collection and Handling:

Warning and Precautions :Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations :1. Heating the medium after acidification should be avoided as it may hydrolyse the agar which can render the agar unable to

solidify.

expiry period

Performance and EvaluationPerformance of the medium is expected when used as per the direction on the label within thewhen stored at recommended temperature.

Quality ControlAppearance

HiMedia Laboratories Technical Data

# Aspergillus brasiliensis ATCC 16404 (00053*)

50-100 good-luxuriant

Candida albicans ATCC10231 (00054*)

50-100 good-luxuriant >=50%

Escherichia coli ATCC25922 (00013*)

>=104 inhibited 0%

Lactobacillus casei ATCC334

>=104 inhibited 0%

Saccharomyces cerevisiae ATCC 9763 (00058*)

50-100 good-luxuriant >=50%

Trichophyton rubrum ATCC28191

50-100 good-luxuriant

Escherichia coli NCTC 9002 >=104 inhibited 0%Escherichia coli ATCC 8739 (00012*)

>=104 inhibited 0%

Cream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gelColour and clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in Petri platesReactionpH of 3.91% w/v aqueous solution at 25°C. pH : 5.6±0.2pH5.40-5.80Cultural ResponseCultural Response was observed at 20-25°C for 2-7 day's. Recovery rate is considered as 100% for fungus growth on Sabouraud Dextrose Agar

Organism Inoculum(CFU)

Growth Recovery

Storage and Shelf Life Store dehydrated powder and the prepared medium in a tightly closed container between 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Use before expiry date on the label. Product performance is best if used within stated expiry period.

Please refer disclaimer Overleaf.

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).

Disposal

Reference

1. American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., WashingtonD.C.

2. FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.

3. Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.4. Jorgensen, J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)

Manual of Clinical Microbiology, 11th Edition. Vol. 1.

5. Lorian (Ed.),1980, Antibiotics In Laboratory Medicine, Williams and Wilkins, Baltimore

6. MacFaddin J. F., 1985, Media for the Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol.1,Williams and Wilkins, Baltimore.

Key : (*) Corresponding WDCM numbers. (#) - Formerly known as Aspergillus niger

Disclaimer :

User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

HiMedia Laboratories Technical Data

8. Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C.

Revision : 01/ 2019

7. Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination ofFoods, 5th Ed., American Public Health Association, Washington, D.C.

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