power pt on cell structure
TRANSCRIPT
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
PowerPoint Lectures for Biology, Seventh Edition
Neil Campbell and Jane Reece
Lectures by Chris Romero
Chapter 6Chapter 6
A Tour of the Cell
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Overview: The Importance of Cells
• All organisms are made of cells
• The cell is the simplest collection of matter that can live
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• Cell structure is correlated to cellular function
10 µm Figure 6.1
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• Concept 6.1: To study cells, biologists use microscopes and the tools of biochemistry
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Microscopy
• Scientists use microscopes to visualize cells too small to see with the naked eye
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• Light microscopes (LMs)
– Pass visible light through a specimen
– Magnify cellular structures with lenses
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• Different types of microscopes
– Can be used to visualize different sized cellular structures
Una
ide
d e
ye
1 m
0.1 nm
10 m
0.1 m
1 cm
1 mm
100 µm
10 µ m
1 µ m
100 nm
10 nm
1 nm
Length of somenerve and muscle cells
Chicken egg
Frog egg
Most plant and Animal cells
Smallest bacteria
Viruses
Ribosomes
Proteins
Lipids
Small molecules
Atoms
NucleusMost bacteriaMitochondrion
Lig
ht m
icro
sco
pe
Ele
ctro
n m
icro
sco
pe
Ele
ctro
n m
icro
sco
pe
Figure 6.2
Human height
Measurements1 centimeter (cm) = 102 meter (m) = 0.4 inch1 millimeter (mm) = 10–3 m1 micrometer (µm) = 10–3 mm = 10–6 m1 nanometer (nm) = 10–3 mm = 10–9 m
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– Use different methods for enhancing visualization of cellular structures
TECHNIQUE RESULT
Brightfield (unstained specimen). Passes light directly through specimen. Unless cell is naturally pigmented or artificially stained, image has little contrast. [Parts (a)–(d) show a human cheek epithelial cell.]
(a)
Brightfield (stained specimen). Staining with various dyes enhances contrast, but most staining procedures require that cells be fixed (preserved).
(b)
Phase-contrast. Enhances contrast in unstained cells by amplifying variations in density within specimen; especially useful for examining living, unpigmented cells.
(c)
50 µm
Figure 6.3
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Differential-interference-contrast (Nomarski). Like phase-contrast microscopy, it uses optical modifications to exaggerate differences indensity, making the image appear almost 3D.
Fluorescence. Shows the locations of specific molecules in the cell by tagging the molecules with fluorescent dyes or antibodies. These fluorescent substances absorb ultraviolet radiation and emit visible light, as shown here in a cell from an artery.
Confocal. Uses lasers and special optics for “optical sectioning” of fluorescently-stained specimens. Only a single plane of focus is illuminated; out-of-focus fluorescence above and below the plane is subtracted by a computer. A sharp image results, as seen in stained nervous tissue (top), where nerve cells are green, support cells are red, and regions of overlap are yellow. A standard fluorescence micrograph (bottom) of this relatively thick tissue is blurry.
50 µm
50 µm
(d)
(e)
(f)
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• Electron microscopes (EMs)
– Focus a beam of electrons through a specimen (TEM) or onto its surface (SEM)
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• The scanning electron microscope (SEM)
– Provides for detailed study of the surface of a specimen
TECHNIQUE RESULTS
Scanning electron micro-scopy (SEM). Micrographs takenwith a scanning electron micro-scope show a 3D image of the surface of a specimen. This SEM shows the surface of a cell from a rabbit trachea (windpipe) covered with motile organelles called cilia. Beating of the cilia helps moveinhaled debris upward toward the throat.
(a)
Cilia1 µm
Figure 6.4 (a)
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• The transmission electron microscope (TEM)
– Provides for detailed study of the internal ultrastructure of cells
Transmission electron micro-scopy (TEM). A transmission electron microscope profiles a thin section of a specimen. Here we see a section through a tracheal cell, revealing its ultrastructure. In preparing the TEM, some cilia were cut along their lengths, creating longitudinal sections, while other cilia were cut straight across, creating cross sections.
(b)
Longitudinalsection ofcilium
Cross sectionof cilium
1 µm
Figure 6.4 (b)
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Isolating Organelles by Cell Fractionation
• Cell fractionation
– Takes cells apart and separates the major organelles from one another
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• The centrifuge
– Is used to fractionate cells into their component parts
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Cell fractionation is used to isolate(fractionate) cell components, based on size and density.
First, cells are homogenized in a blender tobreak them up. The resulting mixture (cell homogenate) is thencentrifuged at various speeds and durations to fractionate the cellcomponents, forming a series of pellets.
• The process of cell fractionation
APPLICATION
TECHNIQUE
Figure 6.5
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Tissuecells
Homogenization
Homogenate1000 g(1000 times theforce of gravity)
10 min Differential centrifugationSupernatant pouredinto next tube
20,000 g20 min
Pellet rich innuclei andcellular debris
Pellet rich inmitochondria(and chloro-plasts if cellsare from a plant)
Pellet rich in“microsomes”(pieces of plasma mem-branes andcells’ internalmembranes)
Pellet rich inribosomes
150,000 g3 hr
80,000 g60 min
Figure 6.5
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In the original experiments, the researchers used microscopy to identify the organelles in each pellet, establishing a baseline for further experiments. In the next series ofexperiments, researchers used biochemical methods to determine the metabolic functions associated with each type of organelle. Researchers currently use cell fractionation to isolate particular organelles in order to study further details of their function.
RESULTS
Figure 6.5
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Concept 6.2: Eukaryotic cells have internal membranes that compartmentalize their functions
• Two types of cells make up every organism
– Prokaryotic
– Eukaryotic
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Comparing Prokaryotic and Eukaryotic Cells
• All cells have several basic features in common
– They are bounded by a plasma membrane
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– They contain a semifluid substance called the cytosol
– They contain chromosomes
– They all have ribosomes
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• Prokaryotic cells
– Do not contain a nucleus
– Have their DNA located in a region called the nucleoid
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(b) A thin section through the bacterium Bacillus coagulans (TEM)
Pili: attachment structures onthe surface of some prokaryotes
Nucleoid: region where thecell’s DNA is located (notenclosed by a membrane)
Ribosomes: organelles thatsynthesize proteins
Plasma membrane: membraneenclosing the cytoplasm
Cell wall: rigid structure outsidethe plasma membrane
Capsule: jelly-like outer coatingof many prokaryotes
Flagella: locomotionorganelles ofsome bacteria
(a) A typical rod-shaped bacterium
0.5 µmBacterial
chromosome
Figure 6.6 A, B
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• Eukaryotic cells
– Contain a true nucleus, bounded by a membranous nuclear envelope
– Are generally quite a bit bigger than prokaryotic cells
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• The logistics of carrying out cellular metabolism sets limits on the size of cells
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• A smaller cell
– Has a higher surface to volume ratio, which facilitates the exchange of materials into and out of the cell
Surface area increases whiletotal volume remains constant
5
11
Total surface area (height width number of sides number of boxes)
Total volume (height width length number of boxes)
Surface-to-volume ratio (surface area volume)
6
1
6
150
125
12
750
125
6
Figure 6.7
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• The plasma membrane
– Functions as a selective barrier
– Allows sufficient passage of nutrients and waste
Carbohydrate side chain
Figure 6.8 A, B
Outside of cell
Inside of cell
Hydrophilicregion
Hydrophobicregion
Hydrophilicregion
(b) Structure of the plasma membrane
Phospholipid Proteins
TEM of a plasmamembrane. Theplasma membrane,here in a red bloodcell, appears as apair of dark bandsseparated by alight band.
(a)
0.1 µm
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A Panoramic View of the Eukaryotic Cell
• Eukaryotic cells
– Have extensive and elaborately arranged internal membranes, which form organelles
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• Plant and animal cells
– Have most of the same organelles
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• A animal cell
Rough ER Smooth ER
Centrosome
CYTOSKELETON
Microfilaments
Microtubules
Microvilli
Peroxisome
Lysosome
Golgi apparatus
Ribosomes
In animal cells but not plant cells:LysosomesCentriolesFlagella (in some plant sperm)
Nucleolus
Chromatin
NUCLEUS
Flagelium
Intermediate filaments
ENDOPLASMIC RETICULUM (ER)
Mitochondrion
Nuclear envelope
Plasma membrane
Figure 6.9
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• A plant cell
In plant cells but not animal cells:ChloroplastsCentral vacuole and tonoplastCell wallPlasmodesmata
CYTOSKELETON
Ribosomes (small brwon dots)
Central vacuole
Microfilaments
Intermediate filaments
Microtubules
Rough endoplasmic reticulum Smooth
endoplasmic reticulum
ChromatinNUCLEUS
Nuclear envelope
Nucleolus
Chloroplast
PlasmodesmataWall of adjacent cell
Cell wall
Golgi apparatus
Peroxisome
Tonoplast
Centrosome
Plasma membrane
Mitochondrion
Figure 6.9
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Concept 6.3: The eukaryotic cell’s genetic instructions are housed in the nucleus and carried out by the ribosomes
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The Nucleus: Genetic Library of the Cell
• The nucleus
– Contains most of the genes in the eukaryotic cell
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• The nuclear envelope
– Encloses the nucleus, separating its contents from the cytoplasm
Figure 6.10
Nucleus
NucleusNucleolus
Chromatin
Nuclear envelope:Inner membraneOuter membrane
Nuclear pore
Rough ER
Porecomplex
Surface of nuclear envelope.
Pore complexes (TEM). Nuclear lamina (TEM).
Close-up of nuclearenvelope
Ribosome
1 µm
1 µm0.25 µm
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Ribosomes: Protein Factories in the Cell
• Ribosomes
– Are particles made of ribosomal RNA and protein
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– Carry out protein synthesis
ER
Endoplasmic reticulum (ER)
Ribosomes Cytosol
Free ribosomes
Bound ribosomes
Largesubunit
Smallsubunit
TEM showing ER and ribosomes Diagram of a ribosome
0.5 µm
Figure 6.11
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Concept 6.4: The endomembrane system regulates protein traffic and performs metabolic functions in the cell
• The endomembrane system
– Includes many different structures
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The Endoplasmic Reticulum: Biosynthetic Factory
• The endoplasmic reticulum (ER)
– Accounts for more than half the total membrane in many eukaryotic cells
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• The ER membrane
– Is continuous with the nuclear envelope
Smooth ER
Rough ER
ER lumenCisternae
RibosomesTransport vesicle
Smooth ER
Transitional ER
Rough ER 200 µm
Nuclearenvelope
Figure 6.12
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• There are two distinct regions of ER
– Smooth ER, which lacks ribosomes
– Rough ER, which contains ribosomes
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Functions of Smooth ER
• The smooth ER
– Synthesizes lipids
– Metabolizes carbohydrates
– Stores calcium
– Detoxifies poison
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Functions of Rough ER
• The rough ER
– Has bound ribosomes
– Produces proteins and membranes, which are distributed by transport vesicles
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• The Golgi apparatus
– Receives many of the transport vesicles produced in the rough ER
– Consists of flattened membranous sacs called cisternae
The Golgi Apparatus: Shipping and Receiving Center
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• Functions of the Golgi apparatus include
– Modification of the products of the rough ER
– Manufacture of certain macromolecules
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Golgiapparatus
TEM of Golgi apparatus
cis face(“receiving” side ofGolgi apparatus)
Vesicles movefrom ER to Golgi Vesicles also
transport certainproteins back to ER
Vesicles coalesce toform new cis Golgi cisternae
Cisternalmaturation:Golgi cisternaemove in a cis-to-transdirection
Vesicles form andleave Golgi, carryingspecific proteins toother locations or tothe plasma mem-brane for secretion
Vesicles transport specificproteins backward to newerGolgi cisternae
Cisternae
trans face(“shipping” side ofGolgi apparatus)
0.1 0 µm16
5
2
3
4
• Functions of the Golgi apparatus
Figure 6.13
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Lysosomes: Digestive Compartments
• A lysosome
– Is a membranous sac of hydrolytic enzymes
– Can digest all kinds of macromolecules
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• Lysosomes carry out intracellular digestion by
– Phagocytosis
Figure 6.14 A(a) Phagocytosis: lysosome digesting food
1 µm
Lysosome containsactive hydrolyticenzymes
Food vacuole fuses with lysosome
Hydrolyticenzymes digestfood particles
Digestion
Food vacuole
Plasma membraneLysosome
Digestiveenzymes
Lysosome
Nucleus
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• Autophagy
Figure 6.14 B(b) Autophagy: lysosome breaking down damaged organelle
Lysosome containingtwo damaged organelles 1 µ m
Mitochondrionfragment
Peroxisomefragment
Lysosome fuses withvesicle containingdamaged organelle
Hydrolytic enzymesdigest organellecomponents
Vesicle containingdamaged mitochondrion
Digestion
Lysosome
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Vacuoles: Diverse Maintenance Compartments
• A plant or fungal cell
– May have one or several vacuoles
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• Food vacuoles
– Are formed by phagocytosis
• Contractile vacuoles
– Pump excess water out of protist cells
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• Central vacuoles
– Are found in plant cells
– Hold reserves of important organic compounds and water
Central vacuole
Cytosol
Tonoplast
Centralvacuole
Nucleus
Cell wall
Chloroplast
5 µmFigure 6.15
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The Endomembrane System: A Review
• The endomembrane system
– Is a complex and dynamic player in the cell’s compartmental organization
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Plasma membrane expandsby fusion of vesicles; proteinsare secreted from cell
Transport vesicle carriesproteins to plasma membrane for secretion
Lysosome availablefor fusion with anothervesicle for digestion
4 5 6
Nuclear envelope isconnected to rough ER, which is also continuous
with smooth ER
Nucleus
Rough ER
Smooth ERcis Golgi
trans Golgi
Membranes and proteinsproduced by the ER flow in
the form of transport vesiclesto the Golgi Nuclear envelop
Golgi pinches off transport Vesicles and other vesicles
that give rise to lysosomes and Vacuoles
1
3
2
Plasmamembrane
• Relationships among organelles of the endomembrane system
Figure 6.16
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• Concept 6.5: Mitochondria and chloroplasts change energy from one form to another
• Mitochondria
– Are the sites of cellular respiration
• Chloroplasts
– Found only in plants, are the sites of photosynthesis
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Mitochondria: Chemical Energy Conversion
• Mitochondria
– Are found in nearly all eukaryotic cells
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• Mitochondria are enclosed by two membranes
– A smooth outer membrane
– An inner membrane folded into cristae
Mitochondrion
Intermembrane space
Outermembrane
Freeribosomesin the mitochondrialmatrix
MitochondrialDNA
Innermembrane
Cristae
Matrix
100 µmFigure 6.17
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Chloroplasts: Capture of Light Energy
• The chloroplast
– Is a specialized member of a family of closely related plant organelles called plastids
– Contains chlorophyll
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• Chloroplasts
– Are found in leaves and other green organs of plants and in algae
Chloroplast
ChloroplastDNA
RibosomesStroma
Inner and outermembranes
Thylakoid
1 µm
Granum
Figure 6.18
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• Chloroplast structure includes
– Thylakoids, membranous sacs
– Stroma, the internal fluid
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Peroxisomes: Oxidation
• Peroxisomes
– Produce hydrogen peroxide and convert it to water
ChloroplastPeroxisome
Mitochondrion
1 µm
Figure 6.19
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Concept 6.6: The cytoskeleton is a network of fibers that organizes structures and activities in the cell
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• The cytoskeleton
– Is a network of fibers extending throughout the cytoplasm
Figure 6.20
Microtubule
0.25 µm MicrofilamentsFigure 6.20
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Roles of the Cytoskeleton: Support, Motility, and Regulation
• The cytoskeleton
– Gives mechanical support to the cell
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– Is involved in cell motility, which utilizes motor proteins
VesicleATP
Receptor formotor protein
Motor protein(ATP powered)
Microtubuleof cytoskeleton
(a) Motor proteins that attach to receptors on organelles can “walk”the organelles along microtubules or, in some cases, microfilaments.
Microtubule Vesicles 0.25 µm
(b) Vesicles containing neurotransmitters migrate to the tips of nerve cell axons via the mechanism in (a). In this SEM of a squid giant axon, two vesicles can be seen moving along a microtubule. (A separate part of the experiment provided the evidence that they were in fact moving.)Figure 6.21 A, B
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Components of the Cytoskeleton
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• There are three main types of fibers that make up the cytoskeleton
Table 6.1
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Microtubules
• Microtubules
– Shape the cell
– Guide movement of organelles
– Help separate the chromosome copies in dividing cells
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Centrosomes and Centrioles
• The centrosome
– Is considered to be a “microtubule-organizing center”
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– Contains a pair of centrioles
Centrosome
Microtubule
Centrioles
0.25 µm
Longitudinal sectionof one centriole
Microtubules Cross sectionof the other centrioleFigure 6.22
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Cilia and Flagella
• Cilia and flagella
– Contain specialized arrangements of microtubules
– Are locomotor appendages of some cells
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• Flagella beating pattern
(a) Motion of flagella. A flagellum usually undulates, its snakelike motion driving a cell in the same direction as the axis of the flagellum. Propulsion of a human sperm cell is an example of flagellatelocomotion (LM).
1 µm
Direction of swimming
Figure 6.23 A
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• Ciliary motion
(b) Motion of cilia. Cilia have a back- and-forth motion that moves the cell in a direction perpendicular to the axis of the cilium. A dense nap of cilia, beating at a rate of about 40 to 60 strokes a second, covers this Colpidium, a freshwater protozoan (SEM).
Figure 6.23 B
15 µm
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• Cilia and flagella share a common ultrastructure
(a)
(c)
(b)
Outer microtubuledoublet
Dynein arms
Centralmicrotubule
Outer doublets cross-linkingproteins inside
Radialspoke
Plasmamembrane
Microtubules
Plasmamembrane
Basal body
0.5 µm
0.1 µm
0.1 µm
Cross section of basal body
Triplet
Figure 6.24 A-C
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• The protein dynein
– Is responsible for the bending movement of cilia and flagella
Microtubuledoublets ATP
Dynein arm
Powered by ATP, the dynein arms of one microtubule doublet grip the adjacent doublet, push it up, release, and then grip again. If the two microtubule doublets were not attached, they would slide relative to each other.
(a)
Figure 6.25 A
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Outer doubletscross-linkingproteins
Anchoragein cell
ATP
In a cilium or flagellum, two adjacent doublets cannot slide far because they are physically restrained by proteins, so they bend. (Only two ofthe nine outer doublets in Figure 6.24b are shown here.)
(b)
Figure 6.25 B
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Localized, synchronized activation of many dynein arms probably causes a bend to begin at the base of the Cilium or flagellum and move outward toward the tip. Many successive bends, such as the ones shown here to the left and right, result in a wavelike motion. In this diagram, the two central microtubules and the cross-linking proteins are not shown.
(c)
1 3
2
Figure 6.25 C
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Microfilaments (Actin Filaments)
• Microfilaments
– Are built from molecules of the protein actin
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– Are found in microvilli
0.25 µm
Microvillus
Plasma membrane
Microfilaments (actinfilaments)
Intermediate filaments
Figure 6.26
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• Microfilaments that function in cellular motility
– Contain the protein myosin in addition to actin
Actin filament
Myosin filament
Myosin motors in muscle cell contraction. (a)
Muscle cell
Myosin arm
Figure 6.27 A
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• Amoeboid movement
– Involves the contraction of actin and myosin filaments
Cortex (outer cytoplasm):gel with actin network
Inner cytoplasm: sol with actin subunits
Extendingpseudopodium
(b) Amoeboid movementFigure 6.27 B
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• Cytoplasmic streaming
– Is another form of locomotion created by microfilaments
Nonmovingcytoplasm (gel)
Chloroplast
Streamingcytoplasm(sol)
Parallel actinfilaments Cell wall
(b) Cytoplasmic streaming in plant cellsFigure 6.27 C
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Intermediate Filaments
• Intermediate filaments
– Support cell shape
– Fix organelles in place
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Concept 6.7: Extracellular components and connections between cells help coordinate cellular activities
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Cell Walls of Plants
• The cell wall
– Is an extracellular structure of plant cells that distinguishes them from animal cells
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• Plant cell walls
– Are made of cellulose fibers embedded in other polysaccharides and protein
– May have multiple layersCentral vacuoleof cell
PlasmamembraneSecondarycell wallPrimarycell wall
Middlelamella
1 µm
Centralvacuoleof cell
Central vacuole CytosolPlasma membrane
Plant cell walls
PlasmodesmataFigure 6.28
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The Extracellular Matrix (ECM) of Animal Cells
• Animal cells
– Lack cell walls
– Are covered by an elaborate matrix, the ECM
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• The ECM
– Is made up of glycoproteins and other macromolecules
Collagen
Fibronectin
Plasmamembrane
EXTRACELLULAR FLUID
Micro-filaments
CYTOPLASM
Integrins
Polysaccharidemolecule
Carbo-hydrates
Proteoglycanmolecule
Coreprotein
Integrin
Figure 6.29
A proteoglycan complex
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• Functions of the ECM include
– Support
– Adhesion
– Movement
– Regulation
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Intercellular Junctions
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Plants: Plasmodesmata
• Plasmodesmata
– Are channels that perforate plant cell walls
Interiorof cell
Interiorof cell
0.5 µm Plasmodesmata Plasma membranes
Cell walls
Figure 6.30
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Animals: Tight Junctions, Desmosomes, and Gap Junctions
• In animals, there are three types of intercellular junctions
– Tight junctions
– Desmosomes
– Gap junctions
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• Types of intercellular junctions in animals
Tight junctions prevent fluid from moving across a layer of cells
Tight junction
0.5 µm
1 µm
Spacebetweencells
Plasma membranesof adjacent cells
Extracellularmatrix
Gap junction
Tight junctions
0.1 µm
Intermediatefilaments
Desmosome
Gapjunctions
At tight junctions, the membranes ofneighboring cells are very tightly pressedagainst each other, bound together byspecific proteins (purple). Forming continu-ous seals around the cells, tight junctionsprevent leakage of extracellular fluid acrossA layer of epithelial cells.
Desmosomes (also called anchoringjunctions) function like rivets, fastening cellsTogether into strong sheets. IntermediateFilaments made of sturdy keratin proteinsAnchor desmosomes in the cytoplasm.
Gap junctions (also called communicatingjunctions) provide cytoplasmic channels fromone cell to an adjacent cell. Gap junctions consist of special membrane proteins that surround a pore through which ions, sugars,amino acids, and other small molecules maypass. Gap junctions are necessary for commu-nication between cells in many types of tissues,including heart muscle and animal embryos.
TIGHT JUNCTIONS
DESMOSOMES
GAP JUNCTIONS
Figure 6.31
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The Cell: A Living Unit Greater Than the Sum of Its Parts
• Cells rely on the integration of structures and organelles in order to function
5 µ
m
Figure 6.32