PP 1-24

PP 25-48

FP 1-24

FP 25-48

SP 1-24

SP 25-48

RP 1-24

RP 25-48

CP 1-24

CP 25-48

DP 1-24

DP 25-48

(A)

(B) PP 4 17 22 11 24 26 38 30 46 47FP 1 19 21 37 38 28 40 42 46 36RP 4 20 9 25 27 28 32 35 47SP 18 20 23 24 38 40 36 48CP 13 16 18 19 39 30 28 31 47DP 14 18 8 9 24 26 41 33 46 47

(C) Marker p-umc1658 amplified DNA fragments in PP46, FP40, SP32, RP38, CP30, and DP24. PP + FP = 46 + 40 = 86 - 48 = RP38 PP + SP = 46 + 32 = 88 - 48 = CP30 FP + SP = 40 + 32 = 72 - 48 = DP2448 is subtracted from any equation total >48 to determine the correct pool number since there are only 48 pools per dimension.Lab Convert translated this clone’s address to 0276H08.

#umc1658(10minimum / 16 candidates /11 resolved)+ indicates unique pool; ? indicates occlusionpp fp sp rp cp dp +? CloneName46 40 32 38 30 24 + b0276H0846 42 32 40 30 26 ? b0276J0846 42 20 40 18 14 + b0275J2038 46 28 36 18 26 ? b0228N0438 28 28 18 18 8 + b0226L0447 37 20 36 19 9 ? b0281E2047 21 20 20 19 41 + b0279E2047 1 32 48 31 33 ? b0278A084 19 27 23 31 46 + b0022C0311 37 20 48 31 9 + b0065E2022 1 25 23 47 26 + b0128A0130 38 9 20 39 47 + b0179F0924 42 4 18 28 46 + b0143J0417 19 47 36 16 18 + b0100C2326 46 35 24 13 33 + b0156N1126 42 4 20 30 46 ? b0155J04

(D)