Katherine Triantafillidou, Georgios Karakinaris

Departme nt of Oral and Maxillofa cial Surgery, Aristotle Univers ity ofThessaloniki , Greece

Purpose : The treatmen t with bisphosphon ates is establishe d incontrolling bone erosion by inhibition of osteocla st action. They are recognized to be useful in the management of osteoporos is, bone metastat ic malignanc y, multiple myelom a and Paget’s disease. The aim of this review is to present the role of BP’s in the manage ment of other intraosseous lesions that cause bone resorpti on.

Materia ls and methods: Compreh ensive reviewing of the literature ,regarding bone lesions, revealed a number of studies where bisphos- phonates could be useful as primary or as adjunctive therapy.

Results : The use of bisphosp honates therapy proved effective for fibrous dysplasia, Gaucher’s disease, chronic recurre nt multifocal osteomy elitis, osteogenesi s imperfec ta and orthopedic implants improvin g osseointegra tion and stabilizati on of implants.

Conclusi ons: BPs are pharmacol ogical agents that inhibit osteo- clastic bone resorption and may be useful in the primary or adjunc- tive treatment of many intraosseou s lesions . Although all the reported studies, in the English literature , concern ing the treatment of such lesions with BPs contain small number of patients, and the most of these are uncontro lled, the results suggest that BPs may have a significant role for the therapy of these lesions. Further inves- tigations are needed to determine the potential role of bisphospho -nates for the treatment of bone lesions.

doi:10.1016/j.oraloncolo gy.2013.03.351

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Mast cells are not related with vascular microd ensity and angi- ogenesis in epithelial dysplasia and oral squamous cell carcinoma sLuciana Maria Pedreira Ramalho, Clarissa Araújo, Gurgel Rocha,Renata Oliveira de Souza, Carolin e Brandi Schlaepfer Sales,Ludmila de Faro Valverde, Jean Nunes dos Santos, Eduardo Antônio,Gonçalves Ramos

Faculdad e de Odontolo gia da UFBA, Canela, Salvador, BA, Brazil

Purpose : Malignant tumors locate d in oral cavity represent 5% ofall human cancers and oral squamous cell carcinoma s (OSCC)correspon d to 90% of these cases. Oral epithelial dysplasia (OED)has a biological potential to progres s to OSCC. The mast cells are well known for stimulating and maintaining the vascular network formation. These cells release factors that stimulate angi- ogenesis such as VEGF-A. Thus, the objective of this study was toevaluate the distribution of mast cell and angiogenesis in OED and OSCC.

Materia l and method s: Fourteen OED and 56 OSCC were immuno -stained for the proteins CD34, VEGF-A, and mast cell tryptase using Envision Advance™ System. Mast cells density (MD-cell/mm 2), and the vascular microde nsity (VMD-vessel/mm 2) were determined bycounting the number of cells and positive vessels for CD34, respec- tively, in five hot spot areas. The expressi on of VEGF-A was evaluate daccordin g to immun ohistochem ical score (Sinicrope et al., 1995). All studied proteins were associat ed with clinical parameters and histo- logical grade of OED and OSCC.

Results : MD, VMD and VEGF-A expressi on were not associat edwith clinical paramet ers and histologi cal grades in OED, and OSCC.The MD and VMD were significantly higher between cases of OED than OSCC (p = 0.01, and p = 0.0003, respectivel y). In OED, there were no association between MD, VMD, and VEGF-A expressi on. In OSCC,

VEGF-A expressi on was positivel y associated with VMD (r = 0.39,p = 0.0299).

Conclusion: In our study, mast cells seem do not contribut e toblood vessel s density and VEGF-A expressi on in OED and OSCC.

doi:10.1016/j.oraloncology.20 13.03.352

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Toluidine blue can be useful to identify severe oral dysplasia with high Ki-67 Labeling index Taı́se Santos , Rebeca Vasconcelos, Bráulio Carneiro,Maria Cristina Cangus sú, Jean Nunes dos Santos,Clarissa Araújo Gurgel, Flávia Caló Xavier de Aquino,Luciana Ramalho

Faculdad e de Odontolo gia da UFBA,Canela , Salvador, BA, Brazil

Purpose: Oral leukoplak ias are oral potentially malignant disor- ders in which toluidine blue can be used to identify important sites to be perform ed a biopsy as these sites could show epithelial dysplas ia.

Material and methods: Thirteen patients with white patches located in the oral mucosa were included in this study. Thus, todeterm ine sites that could show epithelia l dysplasia, toluidin blue was used on the leukopl akias to better guide their biopsies. In addi- tion, all of cases exhibiting epithelia l dysplasia were investigat ed, byimmun ohistochem istry, for Ki-67 and Cyclin d1.

Results: Toluidine blue identified leukoplak ias showing mild,moderat e and severe epithelial dysplasia. Those cases with severe epithelia l dysplasia showed particularly toluidine blue staining (p = 0.061) and increased Ki-67 index (p < 0.001). The histologi cal grading of epithelia l dysplasia and immunosta innig for Cyclin d1were not associat ed (p = 0.506) in this study. It is important to state that all those patien ts have been followed for three years and up todate one case marked as severe dysplasia previously treated bymeans of surgery had changed into an oral squamou s cell carcinoma.

Conclusion: Toluidine blue seems to be useful to guide oral leuko- plakias biopsies showing variables grades of epithelia l dysplasia,especially severe grading, as these alteration s contributed toincrease Ki-67 index.

doi:10.1016/j.oraloncology.20 13.03.353

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Myofibroblasts immunop rofile in the stroma of oral squamous cell carcinoma Lia Pontes Arruda Porto a, Luciana Maria Pedreira Ramalho b,Gardênia Matos Paraguassú a, Fernanda Caires Borba a,Jean Nunes dos Santos b, Adna Conceição Barros a,Maria Cristina Teixei ra Cangussu c, Flávia Caló Aquino Xavier b

a School of Dentistry , Federal University of Bahia, Salvador, BA, Brazil b Departme nt of Propaede utics and Integrat ed Clinic, School of Dentistry,Federal University of Bahia, Salvador, BA, Brazil c Departme nt of Pediatric and Social Dentistry , School of Dentistry,Federal University of Bahia, Salvador, BA, Brazil

Myofibroblasts are known to induce migration and invasion in anumber of contexts both in normal developm ent and tumorige nesis.They have been associated with increased lymph node metastasis as

Asbtracts / Oral Oncology 49 (2013) S93–S156 S131