practical part · 2019-04-06 · associate professor of anatomy and histology bone tissue and...
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Dr. Heba Kalbouneh Associate Professor of Anatomy and Histology
Bone tissue and Ossification Practical part
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Long bone
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Plate ( )
Flat bone
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Decalcified bone
Ground bone
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Compact bone
(Dense or cortical bone)
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Alternating bright and dark bands indicate that collagen fibers in successive lamellae
have different orientations.
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Compact bone
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VOLKMANN’S CANAL
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Cancellous
( trabecular or spongy) bone
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Bone cells
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• Osteoblast Osteocyte
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A: OSTEOBLAST
B: OSTEOCYTE
C: OSTEOID
E: OLD BONE
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Osteocyte
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Osteocytes
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Osteoclasts Howship’s lacuna
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Periosteum: an outer fibrous above the yellow line and inner cellular below the yellow line.
Blue arrow: osteoclast. Red arrow: osteoblast
Green arrow: calcified cartilage. Yellow arrow: Osteocyte
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Osteoid
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A: OSTEOCLAST
B: OSTEOBLAST
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Osteoclasts:
1. Are housed in Howship’s
lacunae. T/F
2. Give rise to osteoblasts. T/F
3. Are located at the site of a
bone resorption. T/F
4. Are derived from
osteoprogenitor cells. T/F
5. Are large multinucleated
cells. T/F
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What tissue type is this? Name the structures indicated by the letters.
Name the ring of matrix at the pointer.
How was this slide prepared?
A
C
B
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What does this space contain?
Are Haversian Canals connected to each other? If so, by what?
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Identify the large black space indicated by the yellow arrow
Identify the small black space indicated by the red arrow
In living tissue, what would occupy this space?
Identify the structure indicated by the yellow circle
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Identify the structure indicated by the pink arrow
Identify the structure indicated by the green arrow
Identify the diagonal black line indicated by the blue arrow
In living tissue, what would occupy this space
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What type of bone is indicated by the pink star?
How many osteons would you expect to find in this particular type of bone?
What type of bone is indicated by the green arrow?
Identify the structure indicated by the black arrow
What previously occurred here?
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Bone Ossification Practical part
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Intramembranous Ossification (prenatal)
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Trabeculum
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trabeculum
osteocyte
Osteoblast
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Hyaline cartilage
Bone collar
Primary
ossification
center
Endochondral Ossification
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Bone collar
E
E
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Zone of ossification
Zone of reserve cartilage
(resting cartilage)
Zone of hypertrophy and
calcification
Zone of growth
(proliferation)
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1
2
3
4
Bone collar
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2
3
4
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1 2 3 4
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Ossification zone
Note bone matrix is acidophilic (red/pink)
while cartilage matrix is basophilic (blue)
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2 3 4
1
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3
2 4
Cartilage at the head of the
bone remains – this is the
articular cartilage (no perichondrium!)
Direction of growth (due to cartilage proliferation)
Shaft (diaphysis) becomes compact bone
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Secondary
center of
ossification
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Growth in the Epiphyseal Plate
48
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What type of bone formation is taking place?
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What type of bone formation is taking place?