preamble: 2.0 purpose: 3.0 scope: 4.0 … · all personnel that work under animal use protocols...

STANDARD OPERATING PROCEDURE

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TITLE: Bio-security at Fish Hatchery Site - Equipment Disinfection & Hand Hygiene Practices

ISSUE DATE: June 13, 2013

APPROVED BY: Vancouver Island University Animal Care Committee Date: 03-25-2014

THIS DOCUMENT IS ONLY VALID AT TIME OF PRINTING, ANY COPIES MADE ARE

CONSIDERED UNCONTROLLED.

Printed on: Wednesday, October 05,

2016

1.0 PREAMBLE:

Premise biosecurity and the biocontainment of animals and potential pathogens are an essential component to the culture of finfish species to ensure proper animal practice. Specific procedures for the disinfection of equipment and personnel hygiene are outlined and practiced in accordance with the Salmonid Health Management Plan recommendations outlined in Appendix IX of the VIU Freshwater/Land-based Aquaculture License as per standards developed by Fisheries and Oceans Canada.

2.0 PURPOSE:

To describe cleaning and disinfection procedures for the following: i) Fish husbandry equipment and tanks ii) Protective clothing iii) Hands iv) Transport equipment v) Trout egg disinfection

3.0 SCOPE:

This procedure applies to all scientists, technicians, animal care and veterinary staff and students at Vancouver Island University. Proper cleaning and disinfection procedures for hands, all protective clothing and fish husbandry equipment, and tanks will greatly reduce the exposure and transfer of parasites and pathogens. Disinfection procedures for equipment and personal protective clothing used off-site must be carefully followed before use in any on-campus aquatic fish culture site (and vice-versa). These procedures apply to any equipment and personal protective clothing used among campus wet labs and tank farms

4.0 RESPONSIBILITIES:

Principal Investigators and/or Responsible Managers have overall responsibility for: ensuring that personnel are aware of and understand this policy/procedure.

All Personnel that work under Animal Use Protocols (AUPs) that cite this SOP are responsible for: a. Familiarizing themselves with this policy/procedure;


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b. Obtaining training for this procedure prior to conducting the procedure (if unsupervised).

5.0 APPROVED PROCEDURE:

5.1 Title of procedure:

Biosecurity at Fish Hatchery Sites - Equipment Disinfection and Hand Hygiene Practices.

5.2 Definition of terms used in the Standard Operating Procedure

PPM = parts per million. 1 ppm = 1 mg L-1 = 1 µl L-1 = 1 g m-3

5.3 Supplies and Equipment:

Virkon™ disinfectant, Ovidine™ disinfectant, Parasite S™, and appropriate containers for disinfectant and cleaning brushes.

5.4 Pre-Procedural Preparations and Examination of Animal:

Hands and equipment must be disinfected before: • Handling Fish • Feeding Fish • Using water quality instruments • Egg Removal • Anaesthetic use for Fish • Euthanasia and Fish Disposal. • Exiting VIU FIsheries and Aquaculture Facilities

5.5 Procedure

On-Campus Equipment Cleaning and Disinfection Procedures a) Hands and Boots: Hands should be disinfected with foaming alcohol hand-rub (dispensers provided at the entry gates to the facilities) and boots disinfected by stepping in disinfectant foot mats when entering or leaving hatcheries and when working in multiple tanks. The soles of boots or shoes should be disinfected prior to entering and exiting hatchery areas by stepping in the disinfectant mats (filled with 1% Virkon™ (Appendix 1) supplied near the entrances to facilities). Directions: For boots: Step in the disinfectant mats which are filled with 1% Virkon™ . For personal hand hygiene: Use disinfectant as required throughout the day or as part of your cleansing routine. Apply 2-3 ml. to clean, dry hands. (Waterless instant


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antiseptics should not be used as a complete substitute for regular hand washing, but as a supplement.) b) Tanks: All tanks must be disinfected with 250 ppm Ovadine™ (Appendix 2) between uses. Tanks must be thoroughly clean of organic debris before disinfection with Ovadine™. Shut off water in-flow to the tank. Ensure that effluent from cleaning and disinfectant application flows to sewer and does not enter the re-circulating water system. Tanks should be scrubbed clean using a brush, abrasive pad or sponge. Apply Ovadine™ using a scrub brush, sponge or spray and allow a one-hour contact time. Spray down tank with de-chlorinated water. Allow tank to air dry before use. c) Nets and Tank Cleaning Brushes: • All nets and tank cleaning brushes must be disinfected after each use with 1% Virkon™. • Handles of all nets and brushes must be labelled with a tank number. • Place nets and brushes into disinfection buckets after use. Allow a 10-minute contact time or keep in Virkon™ disinfection bath overnight. • After disinfection, carefully shake off Virkon™ and rinse nets with de-chlorinated water. All disinfection buckets must be clearly labelled and covered when not in use. A change in Virkon™ color from pink to white indicates a loss in disinfection activity. Ensure that fresh Virkon™ solution is available for daily use. Disinfection buckets for nets and brushes will be available for use in the following areas: Tank Farm # 1 – Hatchery Rearing Area : One large disinfection bucket (60 L plastic garbage can) and one small bucket (20L) will be allocated for the two fresh water re-circulation systems (Cells A and B). One small disinfection bucket (20 L plastic pail) will be allocated for the salt water system (Cell C). Tank Farm #2 : One large disinfection bucket (60 L plastic garbage can) and one small bucket (20L). Chase River S.E.P.: One large disinfection bucket (60 L plastic garbage can). Building 375 – 103 – Tropical Fish Wet Lab: One small disinfection bucket (10 L plastic pail). Building 375 – 107 – Wet Lab: One small disinfection bucket (10 L plastic pail). Building 391 – Sturgeon Hatchery:


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Two small disinfection buckets (20 L plastic pails). a) Fish Holding Buckets, Containers, Heath Trays, Measuring Boards and Weigh Scales. All fish holding buckets and containers must be disinfected after each use with 250 ppm Ovadine. Apply Ovadine™ using a scrub brush, sponge or spray and allow a 20 minute contact time. Rinse well with de-chlorinated water. b) Fish Transport Tank and Truck Following each fish transport (Fish Transportation – SOP) all equipment, transport tank(s), and transport vehicle must be cleaned and disinfected. The transport tank, equipment (nets, buckets) and truck bed must be disinfected with 250 ppm Ovadine™. Apply Ovadine™ using a scrub brush, sponge or spray and allow a 20 minute contact time. Rinse all equipment and the truck with de-chlorinated water. Miscellaneous Equipment Any equipment that may be used in multiple tanks, or among campus wet labs and farm tanks, or used off-site in the field, must be cleaned and disinfected between uses. Care should be taken with making cleaning and disinfection choices for sensitive scientific equipment. Equipment such as DO meters, refractometers, or digital thermometers should be cleaned with a soft cloth sprayed with 1% Virkon™ and allowed to air dry. Whenever possible, equipment should be designated for specific wet lab or tank farm use. Avoid wooden measuring boards as these can accumulate micro organisms – plastic measuring boards are preferred. c) Building 375-102 & 103 – Aquaria and Equipment Disinfection All aquaria and aquarium components must be disinfected with 1500 ppm Parasite S™ (Appendix 3) between uses. Aquaria must be thoroughly clean of organic debris before disinfection with 1500 ppm Parasite S™. Tanks should be scrubbed clean using a brush, abrasive pad or sponge. Apply 1500 ppm™ Parasite S™ using a scrub brush, sponge or spray and allow a one-hour contact time. Rinse tank with de-chlorinated water. Allow aquaria to air dry before use. All nets and tank cleaning brushes must be disinfected after each use with 1500 ppm Parasite S™. Place nets and brushes into Parasite S™ disinfection bath after use. Allow a 20-minute contact time or keep in Parasite S™ disinfection bath overnight. 2) On-Campus Protective Clothing Cleaning and Disinfection Procedures


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All protective clothing or rain gear (boots, waders, and gloves) must be disinfected following use in any campus aquatic wet lab or tank farm. Rain gear requires disinfection with the application of 250ppm Ovidine™ spray. Allow a 10 min. contact time before rinsing off with water. Ovadine™ spray bottles will be readily available in all campus aquatic wet labs, tank farms and at the Chase River SEP Hatchery at times when rain gear or waders will be used. Precaution should be taken to ensure that all protective clothing and rain gear used at off-campus practicum fisheries and aquaculture sites is disinfected prior to use at any campus wet lab, tank farm or the Chase River SEP Hatchery. 3) On-Campus Hand Cleaning and Disinfection Procedures Hands must be cleaned with foaming alcohol handrub (72% ethanol) as a supplement to regular hand-washing before and after handling or feeding fish. Hand sanitizer stations are located in Building 375 – 107(Incubation Room and Wet Lab), Building 375 – 102 (Wet Lab), entrances to Tank Farms #1 and #2, Building 391 (sturgeon rearing area), Building 380-103 (teaching lab), and near the door at Chase River Hatchery. 4) Trout Egg Disinfection Procedure After fertilization, add 100ml of Ovidine ™ to incubator (heath) tray of water (no flow). Restore flow to incubator tray after 10 minutes to flush away Ovidine ™ solution.

5.6 Post- procedural Follow-Up:

Hands and equipment must be disinfected after: • Entering VIU Fisheries and Aquaculture facilities • Handling fish • Feeding fish • Using water quality instruments • Egg removal • Anaesthetic use for fish • Euthanasia and fish disposal

5.7 Safety Equipment and Procedures:

Hand Sanitizer: For external use only. Avoid contact with eyes; if this occurs, flush immediately with water.


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If irritation develops or increases discontinue use of the product. (Advise Technicians and OH&S). If irritation persists for more than five days, consult a doctor. Keep out of reach of children. In case of accidental ingestion, consult a physician or poison control center. MSDS References from Syndel Chemicals web-site: Ovadine MSDS: http://www.syndel.com/msds/ovadine_msds.html Virkon MSDS: http://www.syndel.com/msds/virkon_msds.html Parasite-S MSDS: http://www.syndel.com/msds/parasite-S_msds.htm

MSDS links courtesy of Syndel Laboratories Ltd.

5.8 Non-Conformance:

Any personnel determined to be improperly applying biosecurity procedures will be considered in violation of the associated AUP and will be subject to VIU disciplinary procedure outlined in VIU Procedure 31.01.001 – Animal Care Committee - Section 5.

The VIU Animal Care Committee is authorized by the President of Vancouver Island University to halt any procedure where the committee considers that unnecessary or unforeseen pain or distress is being experienced by an animal, or where use deviates significantly from an approved protocol. Authority extends to humane destruction of the animal if it is considered that pain or distress of the animal cannot be alleviated. The institutional veterinarian is empowered to take the foregoing actions without reference to the researcher or the committee if his/her professional judgement so warrants. (Section 2 of VIU Procedure 31.01.001).

6.0 RECORDS

Records to be completed include: Record of disinfection container recharge is to be completed whenever disinfectant containers are refilled (Appendix 4).


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7.0 FORMS

Forms to be completed include:

n/a

8.0 REFERENCES:

• Canadian Council on Animal Care. 2005. Guidelines on: The care and use of fish in research, teaching and testing. 86 p. • Fish Transportation S.O.P. , Vancouver Island University Further information on the chemicals referred to in this S.O.P. is available at: • Virkon Information Sheet, Syndel Laboratories. Accessed Sept.10, 2008 http://www.syndel.com/d_p_f_s/virkon_info_sheet.html • Ovadine Information Sheet, Syndel Laboratories. Accessed Sept. 10, 2008. http://www.syndel.com/d_p_f_s/ovadine_info_sheet.html • Parasite-S Information Sheet. Syndel Laboratories. Accessed Sept. 10, 2008. http://www.syndel.com/d_p_f_s/parasite-s_info_sheet.htm ACC Terms of Reference: The animal care committee is authorized by the President of Vancouver Island University to halt any procedure where the committee considers that unnecessary or unforeseen pain or distress is being experienced by an animal, or where use deviates significantly from an approved protocol. Authority extends to humane destruction of the animal if it is considered that pain or distress of the animal cannot be alleviated. The institutional veterinarian is empowered to take the foregoing actions without reference to the researcher or the committee if his/her professional judgement so warrants.

9.0 AUTHOR CONTACT INFORMATION:

Daniel Fox - Technician - Fisheries and Aquaculture Department


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REVISION LOG

Revision Date Authority Reviser Revision Details

The

AQUATIC LIFE SCIENCES

Companies

Available in Canada from Syndel Laboratories Ltd (800) 663-2282 www.syndel.com

Available in the USA from Western Chemical Inc (800) 283-5292 www.wchemical.com

Virkon ® Aquatic

Virkon ® Aquatic - Directions for General Use

A 1% Virkon® Aquatic solution is recommended for the cleaning and disinfection of surfaces associated with aquaculture including: vehicles, boats, nets, boots, waders, dive suits & other equipment. Mix the Virkon® Aquatic powder with clean water according to the dilution instructions in the following table. For heavily soiled surfaces, it is recommended to clean with an appropriate detergent prior to disinfection.

Dilution Instructions

% Dilution Required

0.5% (1:200) 1.0% (1:100) 2.0% (1:50)

Final Disinfectant Solution Required

Quantity of Virkon® Aquatic Powder Required

1 liter 5 grams 10 grams 20 grams

5 liters 25 grams 50 grams 100 grams



1. Do not apply Virkon® Aquatic powder directly on surfaces you are trying to disinfect, always mix with

water first.

2. Always make your solution in a clean container of known volume.

3. Measure the correct amount of Virkon® Aquatic powder using the calibrated measuring cup provided.

4. Stir the mixture to dissolve the Virkon® Aquatic powder.

5. Apply the solution to the surfaces to be disinfected, wait for the recommended contact time, and

follow with a clean water rinse.

�� One litre of solution is sufficient to disinfect approximately 4 sq meters.

6. Virkon® Aquatic solutions are stable for up to 7 days. Test strips are available to determine the mixed

solution's strength.

Virkon® Aquatic is US EPA (Reg. No. 71654-6) and Health Canada (DIN # 02276356) approved and is available in 10 lb. (4.53 kg) plastic tubs in the US and in 22 lb (10 kg) plastic tubs in Canada

The


Companies

Ovadine™



DIN 02305712 Ovadine™ is exclusively distributed for fish cultur e by Syndel Laboratories Ltd in Canada and Western Chemical Inc in the USA Ovadine™ is an easy to use, environmentally friendly, general disinfectant. Ovadine™ is a specially buffered, non-staining, non-corrosive, aqueous iodine solution used by fish and shrimp farm personnel as a general disinfectant on equipment, tanks, nets, hands and clothing in hatcheries and at farm sites. Ovadine™ may also be used to disinfect fish and shrimp eggs and shrimp nauplii. Ovadine™ is a fast acting disinfectant that has been shown to be effective against many gram-positive and gram-negative bacteria and fungi. Recommended Dosage & Administration Method General Disinfectant - A 250 ppm available iodine solution is made by diluting 25 ml Ovadine™ to 1 litre with clean water.

Use as a dip or bath. - Wash items that are heavily contaminated with soil or organic debris before disinfecting with

Ovadine™. - A change in the solution colour from dark brown to light yellow indicates loss of activity. Ideally, the

free iodine concentration should be monitored during treatment. Renew by using a fresh solution of Ovadine™.

Fish Egg Disinfectant - Conditions such as the organic content of water and the mass of the fish eggs vary, thus the number

of eggs treated can vary widely. - Place eggs into a 100 ppm free iodine solution of Ovadine™ for ten minutes. A suitable ratio is 1

volume of eggs to 4 volumes of this solution. - A 100 ppm free iodine solution is made by diluting 10 ml Ovadine™ to 1 litre with clean water. Presentation - Ovadine™ is a liquid available in 4 litre, 20 litre and 200 litre containers. Composition - Ovadine™ is a buffered 10% polyvinylpyrrolidone iodine (PVPI) solution in water. The dark brown

liquid contains minimum 1% available iodine. Stability and Storage - PVPI solutions may be stored at room temperature (20-30°C) for periods greater than two years if

containers are kept tightly closed and away from direct sunlight.

The


Companies

Ovadine™



Safety Precautions - PVPI solutions have very low toxicities and are non-irritating to skin, mucosa and wounds. PVPI

solutions are classified as practically non-toxic to humans. There is no evidence of adverse effects from continued use of PVPI solutions.

- Care should be taken when discarding PVPI solutions. For weak solutions, dilute with several volumes of water before discarding.

- For strong solutions, neutralize with sodium thiosulfate before discarding. - Ovadine™ has low toxicity to fish eggs. However, it may be toxic to fish. Therefore, thoroughly rinse

all disinfected surfaces before re-use. - The 100 ppm free iodine solution may be painted on lesions, although it may burn the exposed skin of

some marine fish and smooth skinned fish.

"... .-....

PARASITE-S DANGER 'ft POISON

Formalin (aqueous formaldehyde solution) USER SAFETY WARNINGS

For control of External Protozoa and Monogenetic Trematodes on all Finfish and External

Protozoans on Penaeid Shrimp: and for control of Fungi on all Finfish eggs.

DESCRIPTION

PARAsrTE-S is the aqueous solution of formaldehyde gas (this is equivalent to formalin 37% or

37 grams of formaldehyde in 100mLof solution). U.S.P. grade PARASITE-S contains not less than37%, (by weight) of formaldehyde gas per weight of water and 6 to 14% methanol. In solution,formaldehyde is present chiefly as HO(CH ,o)H. Its molecular weight IS30.93. PARASITE-S isreadily miscible with water, methanol, and ethanol and is slightly soluble in ether. It is a clear,colorless liquid (Heyden Newport Chemical Corporation, 1961).

FISH AND SHRIMP TOXICITY STUDIES

The tOXICityof PARASITE-S was measured by standard methods in laboratory bioassays withrainbow trout, Atlantic salmon, lake trout, black bullhead, channel catfish, green sunfish, bluegill.smallmouth bass. largemouth bass and striped bass. The 3.6,24 and 96-hour LC", (lethalconcentration for 50% of the animals) values for trout range from 1.230 to 100 ).lUL (455 to 37 ppm

tormaldehyde); for catfish, from 495 to 65.8 pUL (183 to 24 ppm formaldehyde); for bluegill, from2,290 to 100 pUL (847 to 37 ppm formaldehyde); for largemouth bass, the values for 6 to 96-hourLC",rangefrom 1,030to 143pUL (381to 53 ppmformaldehyde) (Bill etal. 1977)and forsfnped bassthe values for 6 to 96-hour LC", range from 940 to 30 pUL (347 to 11 ppm formaldehyde) (Bills,Marking & Howe-1993). The 24, 48, 72, and 96-hour LCso values for penaeid shrimp range fro~ 712to 235 ~UL (ppm) (Johnson, 1974 and Williams, 1980).

INDICATIONS FOR USE:

1, Parasiticide lor Finlish: for the control of external protozoa (Chi/odone"a spp., Costia spp.,Epistylis spp., Ichthyophthlrius spp., Scyphidia spp. and Trichodina spp.), and themonogenetic trematode parasites (C/eidodiscus spp., Dacty/ogyrus spp., and Gyrodactylusspp.).

Exposure to high concentrations of formaldehyde vapor causes severe respiratory irritation whichcan be life-threatening. Lowervapor levels can cause irritation to the eyes, respiratory tract. and skin.Swallowing formaldehyde can be life-threatening. Formaldehyde is an irritant when splashed on skinor into the eyes. It can cause severe eye damage, even blindness.

Keep out of reach of children,

Use only with adequate ventilation.

Keep container tightly closed when not in use.

May aggravate a pre-existing asthmatic condition and allergic rhinitis.

Moderate fire and explosion hazard exists when exposed to heat or flame.

Contains methanol - cannot be made non-poisonous. Prolonged exposure to methanol has beenassociated With reproduction disorders.

Potential Cancer Hazard: Formaldehyde vapor may be carcinogenic if inhaled. Use applicable safefyprotection. (Note: This drug, used as labeled, does not cause formaldehyde tissue residues in fish).

Employers: Refer to Occupational Safefy and Health Administration (OSHA) regulation 29 CFR1910.1048 for human safety guidance that may be applicable to your specific operation. OSHA's"action level" concentration for airborne formaldehyde is 0.5 part per million (ppm), calculated as an8-hour time-weighted average (TWA), Use respiratory, skin, and eye protection when needed (referto OSHA's regulation 29 CFR 1910.1048). OSHA's airborne exposure limits (without use of arespirator) for formaldehyde shall not exceed 1) 0.75 part per million (ppm) as an 8-hour,the-weighted average (TWA) or 2) 2 parts per million (ppm) as a 15-minute, short term exposure limit(STEL). NOTE: The odor of formaldehyde in the air can generally be detected at about 0.5 to 0.8ppm (range about 0.05 to 1 ppm).

USER EXPOSURE EMERGENCY AID

1, Parasiticide lor Finlish

Concentrations of Formalin

3, Fungicide lor Finlish Eggs

DIRECTIONS FOR USE:

Store PARASITE-S indoors away from direct sunlight, heat, sparks, and open flames, and ventilatestorage area. Do not subject PARASiTE-S to temperatures below 40'F (4.4'C). PARASITE-Ssubjected to temperatures below 40'F causes the formation of paraformaldehyde, a substancewhich is toxic to fish. Paraformaldehyde can be recognized as a white precipitate at fhe bottom oron the walls of the container.

Tolerance to PARASITE-S may vary with strain and species of fish, eggs and shrimp. While theindicated concentrations are considered safe for fhe indicated use, a small number of each lot to betreated should be used to check for any unusual sensitivity to PARASITE-Sbefore proceeding.

Under some conditions, fish or penaeid shrimp may be stressed by normal treatment concentrations.Heavily parasitized or diseased fish or penaeid shrimp Often have a greatly reduced tolerance toPARASITE-S. Such animals do not tolerate the normal tank treatment regimen the first time they aretreated, Therefore, time and dosage may need to be reduced. If they show evidence of distress (bypiping at the surface), the solution should be removed and replaced with fresh, well aerated water.Careful observations should always be made throughout the treafment periOd whenever tank orraceway treatments are made. Treatment should never exceed 1 hour/or fish or 4 hours for penaeidshrimp (even if they show no sign of distress), nor should it exceed 15 minutes for fish eggs.

Do not apply PARASITE-S to fish ponds, fanks or raceways with water warmer than 27'C (80°F),when a heavy bloom of phytoplankton is present, or when the concentration of dissolved oxygen isless than 5 mg/L (5 ppm). Do not apply to penaeid shrimp ponds when the concentration of thedissolved oxygen is less than 3 to 4 mg/L (ppm). PARASITE-S may kill phytoplankton and can causedepletion of dissolved oxygen. If an oxygen depletion occurs, add fresh, well-aerated water to dilutethe solution and to provide oxygen.

Because formalin may harm a biofilter, biofilters shouid be bypassed during treatment, and thesystem should be flushed and replaced with untreated water before reconnecting the biolilter.

Do not use PARASITE-S in a tank, pond or raceway in which methylene blue, or other dyes whichare absorbed, have been recently used.

INHALATION (Breathing): Vacate exposure area and go to area of fresh air. If removing a victimfrom area of very high vapor concentrations, use a sell-containing breathing apparatus. If the victimis not breathing, give artificial respiration, preferably mouth-to-mouth. Seek medical help immediateiy.

INGESTION (Swallowing): If the person is conscious, dilute, inactivate, or absorb the formaldehydeby giving milk, activated charcoal, or water. Get medical help immediately. If vomiting occurs, keephead lower than hips.

EYE CONTACT: Immediately flush eye(s) with large amounts of water lor at least 15 minutes, liftingthe lower and upper eyelids occasionally, until no evidence 01chemical remains. Seek medicalattention immediately.

SKIN CONTACT: Remove contaminated clothing (including shoes) immediately. Wash affectedarea of body with soap and large amounts of water until no evidence of chemical remains (at least15 minutes). If there are chemical burns, or appreciable eye or respiratory irritation, get medical helpimmediately.

PRECAUTIONS

Administer in Earthen

Ponds indefinitely (pUL)'

25'"

Administer in Tanks and

Raceways for up to 4 hours (J..1UL)·

50 to 100"

Aquatic species

Shrimp

Aquatic species

I Administer in Tanks &

Administer in EarthenRaceways for up to 1 hr

Ponds Indefinitely (pULr(pUL)'

Salmon & troutabove SOc F

up to 170 15-25" •••below 50°F

up to 250 15-25" •••

All other finfish

Iup to 250 ~15-25"'"I

- Mlcrolifer per lifer (pUL) = parts per million (ppm).•• Treat for up to 4 hours daily. Treatment may be repeated daily until parasite control is

achieved. Use the lowerconcentration when ponds, tanks or racewaysare heavily loadedwith phytoplankton, or shrimp, to avoid oxygen depletion due to the biological oxygendemand created by decay of dead phytoplankton. Alternatively, a higher concentrationmight be used if dissolved oxygen is strictly monitored.

•.• Treatment may be repeated in 5 to 10 days, if needed

2. Parasiticide lor Penaeid Shrimp: for fhe control of external protozoan parasites (Bodo spp.,Epistylis spp., and Zoothamnium spp.).

3. Fungicide for Finfish Eggs: for the control of fungi of the family Saprolegniaceae.

Microliter per liter (j.lUL) = parts per million (ppm).Use the lowerconcentration when ponds, tanks or raceways are heavily loadedwith phytoplankton, or fish, to avoid oxygen depletion due to the biological oxygen demand created by decayof dead phytoplankton. Alternatively. a higher concentration might be used if dissolved oxygen

---,s-s-fr~lctry monltorea:-

••• Although the indicated concentrations are considered safe for cold and warm water finfish, asmall numberof each lot or pond to be treated should always be used to check for any unusualsensitivity to formalin before proceeding.

2, Parasiticide lor Penaeid Shrimp



Aquatic species Administer in Hatchery Systems (pULr

Eggs of all finfish except Acipenseriformes I 1000-2000 tor 15 minutes"

Eggs of Acipenseriformes I up to 1500 for 15 minut_e._s·_· _

Microliter per liter (fIUL) = parts per million (ppm).Apply in con~tant flow wfl:tersupply of incubating faCIlities.A preliminary bIoassay shouldbe conducted on a small subsample of fish eggs to determine sensitivity before treatingan entire group. This is necessary for all species because egg sensitivity can vary withspecies or strain and the unique conditions at each facility.

METHODS OF APPLICATION

APPLICATION TO TANKS AND RACEWA YS - Turn off watersupply, provide aeration, applyappropriate amount of PARASITE-S, and thoroughiy dilute and mix to assure equal distribution of PARASITE-S. Treat for up to 1 hour for fish and up to 4 hours for penaeid shrimp, thendrain the solution and refill the tank with fresh, well-aerated water. While tank is undertreatment, adequate oxygen must be present to maintain the fish or shrimp. If needed,aeration should be provided fo prevent oxygen depletion. Treatments may be repeated dailyuntil parasife control is achieved.

APPLICATION TO PONDS - Apply greatly dilufed PARASITE-S to the pond evenly using apump, sprayer, boat bailer, or other suitable device to assure even distribution. Allow

PARASITE-S to dissipate naturally. Singie treatments usually control most parasites, but maybe repeated in 5 to 10 days if needed. Treatmenfs for Ichthyophthirius should be made at2-day intervals until control is achieved.

APPLICATION TO EGG INCUBATORS - Apply PARASITE-S into a constant water supplyflowing around the eggs. A drip or pressure system should be used and timed. ApplyPARASITE-S under the surface of the water flow,

WARNING

ENVIRONMENTAL PRECAUTIONS

Do not discharge the contents of fish treatment tanks into natural streams or ponds withouf thoroughdilution (greater than or equal to lOX). Do not discharge the contents of egg treatment tanks withouta 1OOXdilution. This will avoid damage fo PARASITE-S sensitive phytoplankton, zooplankton, andfish populations and avoid depletion of dissolved oxygen.

Formaldehyde is identified by the U.S. Environmental Protection Agency (EPA) as a toxic pollutantand hazardous substance and is required by regulation (40 CFR, Part 122) to be identified as adischarge for NPDES permits for aquatic animal production laCilities~aquaculture projects and otherfacilities, Formaldehyde is subject to SARA Title III, Secfion 313 reporfing.

Use, storage, and disposal 01this product must be handled in accordance with applicable local, stateand Federal laws.

STORAGE

Recommended storage temperature 59'F (15'C). DO NOT EXPOSE TO DIRECT SUNLIGHT,Store PARASITE-S indoors away from direct sunlighf, heat, spark, and open flame, and ventilatestorage area. Do not subject PARASITE-S to temperatures below 40'F (4.4'C).

Manufactured for:

Western Chernicallnc.

1269 Lattimore Rd., Ferndale, WA 98248(360) 384-5898

NADA 140-989, Approved by FDA

Striped bass have been demonsfrated to be hypersensitive fo formalin; lethal toxicity hasbeen noted to occur at levels approximately 2-3 times the recommended therapeuticconcentration.

Record of Disinfectant Recharge - Vancouver Island University Hatchery Sites

Legend:

A - disinfectant baths (60L trash can and 20L bucket) Building 375 Tank Farm 1

B - disinfectant baths (60L trash can and 20L bucket) Building 375 Tank Farm 2

C - Foot mats Building 375 Tank Farm1, Tank Farm 2, Room 103, Room 108, Building 391 (greenhouse)

Note: All disinfectant baths are to be recharge with 1% Virkontm solution

For 60L buckets: Use 400 g Virkon for 40L of water

For 20L buckets: Use 200 g Virkon for 20L of water

For foot mats: Use 60 g for 6L of water (each mat)

Date Recharged (use legend) Initials Comments

preamble: 2.0 purpose: 3.0 scope: 4.0 … · all personnel that work under animal use protocols...

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