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Preparative Columns

PREPARATIV E LC COLUMNS

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Introduction .......................................................................................................................................................................201Preparative LC Columns and Consumables ......................................................................................................................... 201

Waters OBD Column Design........................................................................................................................................... 202Column Stability and Reliability — Long, Predictable Lifetimes ......................................................................................202High Throughput in the Purification Laboratory.................................................................................................................203

XSelect OBD Preparative Columns................................................................................................................................ 206

XBridge BEH OBD Preparative Columns ...................................................................................................................... 207A Milestone in Chromatography .........................................................................................................................................207XBridge Prep C18 and C8 .....................................................................................................................................................207XBridge BEH Shield RP18 ...................................................................................................................................................207XBridge BEH Phenyl ............................................................................................................................................................208XBridge BEH Amide and XBridge BEH HILIC .....................................................................................................................208

SunFire OBD Preparative Columns .................................................................................................................................210Normal-Phase SunFire Silica OBD Preparative Columns .....................................................................................................212

Atlantis OBD Preparative Columns ................................................................................................................................213Atlantis dC18 .................................................................................................................................................................213Atlantis T3 .............................................................................................................................................................................213Atlantis HILIC Silica .............................................................................................................................................................. 214

XTerra OBD Preparative Columns ...................................................................................................................................215XTerra MS .............................................................................................................................................................................215XTerra RP ..............................................................................................................................................................................215

Symmetry Columns ..........................................................................................................................................................216SymmetryPrep .......................................................................................................................................................................216SymmetryShield ....................................................................................................................................................................216Symmetry300 .......................................................................................................................................................................216

µBondapak/Bondapak and µPorasil/Porasil Columns ................................................................................................217

Waters Spherisorb Columns ............................................................................................................................................218

Prep Nova-Pak HR Columns .............................................................................................................................................219

Preparative Bulk Material ...............................................................................................................................................219

Delta-Pak Columns .......................................................................................................................................................... 220

Radial Compression Modules ..........................................................................................................................................2218 x10 Holder ........................................................................................................................................................................ 221PrepLC 25 mm Module ........................................................................................................................................................ 221PrepLC Assemblies ............................................................................................................................................................... 221

PrepPak Cartridges.......................................................................................................................................................... 222

Purification and Isolation Cartridge Holders............................................................................................................... 222

Accessories and Spare Parts for Waters Radial Compression Cartridge Holders and Modules ............................ 222

How Do You Know Your Chromatographic System is in Proper Working Order? ......................................................224

TABLE OF CONT ENTS


Preparative Columns

Column Performance — Identifying the Problem

Laboratory-scale HPLC purification presents many challenges to the chromatographer. One of the most frustrating challenges relates to the preparative column itself. Inconsistencies in column-to-column performance and lifetimes often result in lost samples, repeat purification runs, and poor scalability from small- to large-volume columns. Scientists at Waters recognized this problem and, over a 3-year period, studied all aspects of the packing processes as well as the column design. Launched in 2003, the Waters proprietary* Optimum Bed Density (OBD) design is a direct result of these investigations.

For a column to remain stable during operation, the bed must be packed sufficiently dense to withstand the compressive fluid forces encountered during use. In the case of analytical column dimensions, the necessary packed-bed density can generally be achieved using traditional slurry packing methods. As the diameter and length of the column increases, it becomes increasingly difficult to reach the bed density required for stable, long-term performance when using small particles. Optimizing the bed density depends on the specific properties of the chromatographic particles and column design being used.

* US Patent # 7,399,410, UK Patent # GB2408469

Average Bed Density for Conventional Analytical LC-Column-Slurry-Packing Procedures vs. Column Aspect Ratio

0.550

0.575

0.600

0.625

0.650

0.675

0.700

Column Aspect Ratio — Length/Diameter

4.6 x 100 mm

19 x 100 mm

19 x 50 mm

50 x 100 mm

Standard Packing Procedures Insufficientto Achieve Optimum Packed-Bed Densitiesin Larger-Diameter Columns.

Optimum Packed-Bed Density

Pac

ked-

Bed

Den

sity

[g/c

m3 ]

2520151050

Preparative LC Columns

Analytical LC Columns

INTRODUCTION

Preparative LC Columns and Consumables

Productivity Comes From Predictability


With careful tuning of the packing process for each particle type and column geometry, the Preparative OBD design and process result in predictable, uniform density profiles throughout the column. During the final capping process, Waters Column Packing Operation follows carefully established procedures designed not to over-compress or disrupt, in any non-uniform way, this portion of the bed. Waters scientists have established that too much axial compression applied at the inlet can break particles, build bridges, and lower local bed permeability.

The Preparative OBD Column is designed to incorporate a pair of specially-designed distributors and chemically-inert seals made to prevent leaks at high operating pressures.

WATERS OBD COLUMN DESIGNWaters has combined high-pressure slurry packing with a carefully calculated axial compression element localized at the less-dense inlet end of the bed.

Packed-bedDensity Axial-Ram-Compressed

Slurry Packed

High-PressureSlurry Packed

Optimum Bed Density

Column OutletColumn Inlet

Effect of packing method on average bed density across column length.

High-Pressure Slurry PackingLocalized Bed Expansion Near Inlet

OBD ProcessOptimum Bed Density Throughout

Axial-Ram Slurry PackingLocalized Compression Near Inlet

Optimized bed density in an OBD Column is achieved by judicious application of a tuned amount of axial force only at the column inlet.

Column Stability and Reliability — Long, Predictable LifetimesThe demand for rapid high purity compound isolation places strong emphasis on the integrity and stability of the preparative column. Complex, sparingly-soluble starting materials are often dissolved with strong solvents, such as DMSO. This combination of poor solubility and pressure shocks associated with large injection volumes of pure organic solvent are the primary contributors to early column failure and chromatographic bed collapse.

The OBD design exhibits exceptional resistance to mechanical chromatographic bed failure and delivers consistent column-to-column performance, reducing cost through extended lifetimes.

Injection 1 Injection 7,000

Data from a High-Throughput Drug Discovery Laboratory: 7000 Injections on an XBridge BEH C18 OBD Prep Column, 5 µm, 19 x 50 mm

An exploded view of the elements of an empty OBD Column.


Preparative Columns

To try this tool, go to

www.waters.com/prepcalculator

Waters Preparative OBD Columns Calculator Convenient scale-up tool that provides:

Mass load scaling

Gradient scaling with appropriate flow rate scale-up and predicting volume consumption

Calculations for split flow ratios for those using mass spectrometer driven chromatography

Focused gradient UPLC to preparative method transfer

UPLC MethodColumn: ACQUITY UPLC CSH C18, 1.7 µm, 2.1 x 30 mmPart number: 186005295 Mobile phase A: 0.1% ammonium hydroxide in waterMobile phase B: 0.1% ammonium hydroxide in acetonitrileUPLC gradient: 2 to 98% B in 2.0 min., then 0.5 min. holdFlow rate: 0.9 mL/minTemp.: 30 °CUV detector: 210 and 254 nm ToF-MS (in ES+ mode)

HPLC Prep MethodColumn: XSelect CSH C18 OBD Prep, 5 µm, 19 x 100 mm Part number: 186005421 Gradient: 2 to 30% B in 0.81 min., 40% B at 10 min., 98% B at 11 min., hold for 1.0 min., and reset conditions for 3.0 min.Flow rate: 25.0 mL/minTemp.: AmbientPurification system: AutoPurification™ Prep HPLC with FractionLynx™Detection: UV at 210 and 254 nm and ESI+ MS

Compounds:Sample 4: 5-hydroxyindole acetic acid acetylation productsTarget peak (UPLC): 1.06 min., m/z 216Mass-directed purification (50 mg): target mono-acetylated product at m/z 216

0.0 0.5 1.0 1.5 2.0 2.5 min

AU

0.0

5.0e-1

1.0 1.07

Determine Prep HPLC Method and Collect Fraction XSelect CSH C18 0.1% Ammonium Hydroxide

Rapid Screening by UPLC ACQUITY UPLC CSH C18 0.1% Ammonium Hydroxide

0.0

0.0

0.5 1.0 1.5 2.0 2.5 min

AU

0.0

5.0e-1

1.0

1.5

1.06

0.51

0.67

0.78 1.27

Confirm Purity UPLC Screening Method 0.1% Ammonium Hydroxide

2 4 6 8 10 12 14 min

AU

0.0

1.0e+2

2.0e+2

4.75

1.52

1.02

1.67

10.72 7.10 11.45

m/z=216.0

m/z 216

Screening (top), purification (middle), and purity confirmation (bottom) for 5-hydroxy-indoleacetic acid reaction products. The UPLC screening result with the target peak at 1.06 minutes (m/z 216) was transferred to preparative HPLC using a focused-gradient (50 mg loading). Mass-directed purification of the target peak results in a pure product, confirmed by analyzing the purified product using the UPLC screening protocol.

High Throughput in the Purification LaboratoryImproving throughput in the purification laboratory is a constant goal. Many laboratories are moving to the ACQUITY UPLC System for faster screening and added resolution. In this example, the UPLC screening method (linear gradient) was scaled directly to a focused gradient prep run to create a fast and more efficient preparative separation. T he Waters Focused Gradient UPLC to Prep Calculator tool (www.waters.com/prepcalculator) assisted in scaling the method from sub-2-µm columns to prep HPLC columns. Laboratories can move quickly from analytical screening to shorter purification runs by using focused gradients and the same column chemistry in both analytical and prep column formats.

Confirm Purity by UPLC

Rapid Screening by UPLCUPLC linear gradient method for fast screening of purification samples. Determine elution time of target compound that is used to determine focused gradient method for purification run.

time

% o

rgan

ic

% o

rgan

ic

time

Determine HPLC Purification MethodCalculate Prep HPLC focused gradient method from the UPLC screen using the Waters Focused Gradient UPLC to Prep Calculator.

Collect FractionsPurification run with Prep HPLC focused gradient method.

¡Determine system volumes

¡Enter elution time from screening run

time

% o

rgan

ic

% o

rgan

ic

time

http://www.waters.com/waters/partDetail.htm?partNumber=186005295



Selecting the Column Packing

Waters column packing materials bring you the best in preparative chromatography options. For optimum flexibility, our column packing materials are available in a range of steel columns. A complete list of preparative packing materials and their characteristics are shown in the following table.

Packing ChemistryParticle

SizePore Size

Particle Shape

Carbon Load

End Capped

XSelect

CSH C18 5 µm, 10 µm 130Å Spherical 15% Yes

CSH Fluoro-Phenyl

5 µm, 10 µm 130Å Spherical 10% No

CSH Phenyl-Hexyl 5 µm 130Å Spherical 14% Yes

HSS C18 5 µm 100Å Spherical 15% Yes

HSS C18 SB 5 µm 100Å Spherical 8% No

HSS T3 5 µm 100Å Spherical 11% Yes

XBridge

C18 5, 10 µm 130Å Spherical 18% Yes


Shield RP18 5, 10 µm 130Å Spherical 17% Yes

Phenyl 5 µm 130Å Spherical 15% Yes

HILIC 5 µm 130Å Spherical N/A No

Amide 5 µm 130Å Spherical 12% No

SunFire



Silica 5, 10 µm 100Å Spherical N/A No

Atlantis

T3 5, 10 µm 100Å Spherical 14% Yes

dC18 5, 10 µm 100Å Spherical 11% Yes

HILIC 5 µm 100Å Spherical N/A No

XTerra MSC18 5, 10 µm 125Å Spherical 16% Yes


XTerra RPC18 5, 10 µm 125Å Spherical 15% Yes


SymmetryC18 5, 7 µm 100Å Spherical 19% Yes


Symmetry-Shield



Preparative Packing Characteristics

Packing ChemistryParticle

SizePore Size

Particle Shape

Carbon Load

End Capped

Symmetry 300

C18 5 µm 300Å Spherical 9% Yes

Prep Nova-Pak HR


Silica 6 µm 60Å Spherical N/A N/A

µBondapak

C18 10 µm 125Å Irregular 10% Yes

Phenyl 10 µm 125Å Irregular 8% Yes

CN 10 µm 125Å Irregular 6% Yes

NH2 10 µm 125Å Irregular 4% No

Bondapak

C18 15–20 µm 125Å Irregular 10% Yes



Bondapak HC18 HA


µPorasil Silica 10 µm 125Å Irregular N/A N/A

PorasilSilica 15–20 µm 125Å Irregular N/A N/A

Silica 37–55 µm 125Å Irregular N/A N/A

Delta-Pak





PrepC18 55–105 µm 125Å Irregular 11% Yes

Silica 55–105 µm 125Å Irregular N/A N/A

Waters AutoPurification SystemNo matter how you collect fractions, whether by MS, UV, ELS, or other detector signals, the Waters AutoPurification Systems will meet your needs. These systems provide simplicity, scalability, and speed with a variety of available configurations along with a standard to verify correct fraction collection (see page 430). The market leading FractionLynx Application Manager provides an intuitive interface to manage the purification process from sample acquisition to reporting.

Pictured here is the Waters AutoPurification System with a 2767 Sample Manager for injecting/collecting/re-injecting, 2545 Binary Gradient Module for high pressure mixing, System Fluidics Organizer for software-controlled valving, fluidics, and column management, 2998 PDA, 2424 ELS, and 3100 Mass Detectors.


Preparative Columns

STEP 3 Once the desired purified mass is established, some simple equations may be used to determine the required column size for purification.

In addition, preparative HPLC system maximum flow rate and backpressure need to be considered and can limit column size.

STEP 2Determine how much mass you need to purify or isolate.

STEP 1Once the analytical separation has been optimized, a loading study on the analytical column is performed to determine the capacity of the

particular packing material. Because the large-scale separation should be identical to the small-scale separation, the maximum sample load will be dependent upon the complexity of the analytical separation.

Scale-up Factor

Scale-up factor =(Diameter preparative)2 x Length preparative

(Diameter analytical)2 x Length analytical

Consider scaling up from a 4.6 x 150 mm column to a 19 x 150 mm column:

Scale-up factor =(19)2 x 150

= 17.1(4.6)2 x 150

Applying the scale-up factor, we can predict that approximately 17–135 mg of sample could be applied to the larger column (packed with the same material as the analytical column). This range is based on an analytical (4.6 mm I.D.) mass load of 1–8 mg.

Flow Rate

Flow rate (prep) = Flow rate (analytical) x

Diameter (prep)2

x

Particle Size (analytical)

Diameter (analytical)2

Particle Size (prep)

The calculated flow rate may be used for the larger column to ensure the same linear velocity of mobile phases as used in the analytical run. However, reasonable flow rates are based on column diameters. Systems will be limited by increasing backpressure with increasing column length and decreasing particle size.

Gradient Duration (GD)

GD (prep) =

GD (analytical) x Length (prep)

x

Diameter (prep)2

x

Flow rate (analytical)

Length (analytical)

Diameter (analytical)2

Flow rate (prep)

Many factors affect the mass capacity of preparative columns. The listed capacities represent an ‘average’ estimate.

Capacity is:

¡ Higher for strongly retained materials

¡ Higher for simple mixtures

¡ Lower where higher resolution is required

¡ Very strongly dependent on loading conditions – limited by loading volume – limited by diluent solvent strength

Reasonable flow rates are based on column diameter. Systems will be limited by increasing backpressure with increasing column length and decreasing particle size.

Reasonable injection volumes are based on column diameter at a length of 50 mm with relatively strong solvents. Increased length is compatible with larger injection, but not proportionately so. Weaker solvents significantly increase injection volume.

Mass loading capacities for peptide purifications depend strongly on the sequence and may be estimated at 5–20% of listed values.

Choosing Preparative Columns Mass Loading

Approximate Mass Loading Capacity (mg) for OBD Preparative Columns (Gradient Mode)

Diameter (mm)

Length (mm) 4.6 10 19 30 50

50 3 15 45 110 310

75 — — — 165 —

100 5 25 90 225 620

150 8 40 135 335 930

250 13 60 225 560 1550

Reasonable Flow Rate (mL/min) 1.4 6.6 24 60 164

Reasonable Injection Volume (µL) 20 100 350 880 2450

The calculated preparative gradient duration is entered into the pump’s gradient separation over the same number of column volumes as was used in the analytical run.

Did you know...Waters offers a Preparative Chromatography Mix Standard to help you benchmark the performance of your preparative/purification system.

For more information, see page 224.


10 min0.00

0.50

1.00

1.50

1 3 2.00

Prep Purification100 mg total load

Collected Fraction

AU

1.00

1.50

Pooled purified fractionsDiphenhydramine = 100% purity

0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 min

AU

1

3

0.00

0.50

1.00

1.50

AU

Crude mixDiphenhydramine = 56.3% purity

0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 min

11

3

Target Analyte

10 min0.00

0.500.5

1.00

1.500

1 3 00

Prep Puuuru ificatioooon100 mg ttttotal loaaaad

Collected Fraction

1.00

1.50

0.0 0.2 22 2 0.4

AU

1

3

Crude mixDiphenhydramine = 56.3% purity

8 2.0 min

3

2. Scale UPLC separation to preparative column scale

3. Run preparative separation using focused gradient

4. Collect and isolate fraction(s) of interest

5. Confirm fraction purity using UPLC Technology1. Develop method using UPLC Technology

XSELECT OBD PREPARATIVE COLUMNSCharged Surface Hybrid (CSH) Technology, especially when combined with the analytical method development capabilities of UPLC Technology and the industry-leading column lifetimes offered by OBD Columns, may have the greatest benefits to isolation and purification scientists. This is because CSH Technology offers the highest loading of basic compounds separated under the volatile, acidic, low pH mobile-phase conditions often preferred by isolation and purification scientists. This increased loadability allows for the use of a narrower (lower volume) preparative column, thereby reducing solvent consumption and fraction volumes.

¡ XSelect CSH C18

¡ XSelect CSH Phenyl-Hexyl

¡ XSelect CSH Fluoro-Phenyl

The scalability built into ACQUITY UPLC CSH and XSelect CSH Columns permits the use of UPLC Technology as a powerful method development tool for the isolation and purification scientist. How can chromatographic separation platforms that appear to be on opposite ends of the analysis scale spectrum (microbore vs. preparative) complement each other? Again, the answer lies in scalability and CSH Technology’s resistance to ‘pH switching’ column performance degradation. Fast, robust, analytical-scale separations can be easily and quickly developed using UPLC Technology, ACQUITY UPLC CSH Columns and simple mobile-phase additives and then straightforwardly scaled and transferred to an XSelect OBD Prep Column. In addition, the purity check of the isolated fraction(s) and/or structural analysis of isolated impurities can be determined using UPLC-MS.

No other manufacturer can offer the selectivity, loadability, and scalability of CSH Technology with the throughput, sensitivity, and transferable analytical-scale method development of UPLC Technology. XSelect HSS OBD Prep Columns (HSS C18, HSS C18 SB, and HSS T3) are available in 10 mm I.D. to assist scaling from UPLC to small scale Prep.

Using CSH Technology throughout the entire process, methods can be developed quickly with ACQUITY UPLC CSH Columns and UPLC Technology and then transferred to preparative-scale OBD XSelect Columns for isolation and purification. The purity of the isolated fraction(s) can then be measured/confirmed using ACQUITY UPLC CSH Columns and UPLC Technology.

LC ConditionsColumns: ACQUITY UPLC CSH C18, 1.7 μm, 2.1 x 50 mm Part number: 186005296Mobile phase A: 0.1% formic acid in waterMobile phase B: 0.1% formic acid in acetonitrile Gradient: Time Flow Profile (min) (mL/min) %A %B Curve Initial 0.9 95 5 Initial 2.00 0.9 5 95 6 2.50 0.9 5 95 6 2.51 0.9 95 5 6 3.50 0.9 95 5 6Injection vol.: 1 μLSample conc.: 800 µg/mL for crude mixSample diluent: 50/50 methanol/waterColumn temp.: 40 °CWeak needle wash: 95/5 water/acetonitrile Strong needle wash: 95/5 acetonitrile/waterDetection: UV @ 220 nmSampling rate: 20 points/secFilter time Constant: 0.1 secSystem: ACQUITY UPLC with ACQUITY UPLC PDA Detector

Preparative ConditionsColumn: XSelect CSH C18 OBD Prep, 5 μm, 19 x 100 mm Part number: 186005421Mobile phase A: 0.1% formic acid in waterMobile phase B: 0.1% formic acid in acetonitrile Gradient: Time Flow Profile (min) (mL/min) %A %B Curve 1.21 25 95 5 Initial 1.71 25 95 5 6 7.02 25 78.8 21.2 6 7.21 25 70.8 29.2 6 8.21 25 5 95 6 8.31 25 95 5 6 13.21 25 95 5 6

Injection vol.: 1.25 mLSample conc.: 80 mg/mL for crude mixDiphenhydramine: 40 mg/mLOxybutynin: 16 mg/mLTerfenadine : 24 mg/mLSample diluent: DMSOColumn temp.: 40 °CWeak needle wash: 95/5 water/methanol Strong needle wash: 95/5 methanol/waterDetection: UV @ 220 nmSampling rate: 1 point/secFilter time constant: 1 secSystem: Waters 2525 Binary Gradient Module, 2767 Sample Manager, Column Fluidics Organizer, 2996 Photodiode Array Detector, ZQ™ Mass Spectrometer

Columns Designed for Isolation and Purification

Compounds:1. Diphenhydramine2. Oxybutynin3. Terfenadine

XSelect Peptide CSH C18 5 µm Prep Columns can be found in the Peptide Purification section.




Preparative Columns

XSelect Preparative ColumnsDimension Type Particle Size CSH C18 CSH Fluoro-Phenyl CSH Phenyl-Hexyl HSS C18 HSS C18 SB HSS T3 CSH C18 10 µm

10 x 10 mm Guard 5 µm 1860054911 1860054981 1860055051 1860047761 1860047581 1860047951 1860072851

10 x 50 mm Column 5 µm 186005414 186005427 186005440 186004778 186004760 186004797 186007286

10 x 100 mm Column 5 µm 186005415 186005428 186005441 186004779 186004761 186004798 186007287

10 x 150 mm Column 5 µm 186005416 186005429 186005442 186004777 186004759 186004796 186007288

10 x 250 mm Column 5 µm 186005417 186005430 186005443 — — 186007515 186007289

19 x 10 mm Guard 5 µm 1860054182 1860054312 1860054442 — — — 1860072902

OBD 19 x 50 mm Column 5 µm 186005420 186005433 186005446 — — — 186007291

OBD 19 x 100 mm Column 5 µm 186005421 186005434 186005447 — — — 186007292

OBD 19 x 150 mm Column 5 µm 186005422 186005435 186005448 — — — 186007293

OBD 19 x 250 mm Column 5 µm 186005492 186005499 186005506 — — — 186007294

30 x 10 mm Guard 5 µm 1860068993 1860069003 1860069013 — — — 186007295

OBD 30 x 50 mm Column 5 µm 186005423 186005436 186005520 — — — 186007296

OBD 30 x 75 mm Column 5 µm 186005424 186005437 186005450 — — — 186007297

OBD 30 x 100 mm Column 5 µm 186005425 186005438 186005451 — — — 186007298

OBD 30 x 150 mm Column 5 µm 186005426 186005439 186005452 — — — 186007299

OBD 30 x 250 mm Column 5 µm 186005493 186005500 186005507 — — — 186007300

OBD 50 x 50 mm Column 5 µm 186005494 186005501 186005508 — — — 186007301

OBD 50 x 100 mm Column 5 µm 186005495 186005502 186005509 — — — 186007302

OBD 50 x 150 mm Column 5 µm 186005496 186005503 186005510 — — — 186007303

OBD 50 x 250 mm Column 5 µm 186005497 186005504 186005511 — — — 186007304 1 Requires 10 x 10 mm Prep Guard Holder, p/n: 2890007792 Requires 19 x 10 mm Prep Guard Holder, p/n: 1860007093 Requires 30 x 10 mm Prep Guard Holder, p/n: 186006912

XBRIDGE BEH OBD PREPARATIVE COLUMNS

A Milestone in ChromatographyFirst introduced in 1999, Waters patented Hybrid Particle Technology (HPT) surmounted significant limitations of silica-based, reversed-phase packing materials, particularly their hydrolytic instability at high pH. In 2005, the introduction of a second generation HPT, branded as XBridge OBD Prep Columns, marked a new milestone in chromatography. With an order of magnitude improvement in high pH stability and a higher level of chromatographic performance, XBridge OBD Prep Columns define the new benchmark for LC purification.

XBridge Prep C18 and C8 C18 and C8 phases remain to be the workhorse of the purification chemist. The second generation HPT phases deliver not only unmatched pH stability, but also an equivalent chromatographic performance and peak efficiency to that of the best silica-based product. This is a testament to the enhanced mass transfer characteristics of this particle. These attributes, combined with the OBD packing design, make the XBridge Prep C18 and C8 phases ideal for robust purification procedures.

XBridge BEH Shield RP18The XBridge BEH Shield RP18 phase combines the BEH particle with the Waters patented Shield Technology. T his embedded polar group reversed-phase material delivers maximum peak efficiency and a unique alternate selectivity, particularly useful in the isolation of phenolic analytes. An additional feature of this phase is the ability to routinely operate in 100% aqueous mobile phases without exhibiting the problems associated with phase dewetting.

XBridge Peptide BEH C18 130Å and 300Å and XBridge Protein BEH C4 300Å Prep Columns can be found in the Bioseparation section of the catalog.


































































































XBridge Preparative ColumnsDimension Type Particle Size C18 C8 Shield RP18 Phenyl HILIC Amide

10 x 10 mm Guard 5 µm 1860029721 1860029911 1860029831 1860033541 1860047201 1860065971

10 x 50 mm Column 5 µm 186002973 186003264 186003257 186003271 186004721 186006599

10 x 100 mm Column 5 µm 186003255 186003265 186003258 186003272 186004722 186006600

10 x 150 mm Column 5 µm 186002974 186003266 186003259 186003273 — 186006601

10 x 250 mm Column 5 µm 186003256 186003267 186003260 186003274 — 186006602

19 x 10 mm Guard 5 µm 1860029752 1860029922 1860029842 1860033552 1860047232 1860065982

OBD 19 x 50 mm Column 5 µm 186002977 186002993 186002985 186003356 186004724 186006603

OBD 19 x 100 mm Column 5 µm 186002978 186002994 186002986 186003357 186004725 186006604

OBD 19 x 150 mm Column 5 µm 186002979 186002995 186002987 186003358 186004726 186006605

OBD 19 x 250 mm Column 5 µm 186004021 186004023 186004022 186004024 186004730 186006606

30 x 10 mm Guard 5 µm 1860068933 1860068953 1860068983 1860068913 1860068963 1860068903

OBD 30 x 50 mm Column 5 µm 186002980 186002996 186002988 186003277 186004727 186006607

OBD 30 x 75 mm Column 5 µm 186002981 186003269 186003262 186003278 — 186006608

OBD 30 x 100 mm Column 5 µm 186002982 186002997 186002989 186003279 186004728 186006609

OBD 30 x 150 mm Column 5 µm 186003284 186003083 186002990 186003276 186004729 186006610

OBD 30 x 250 mm Column 5 µm 186004025 — — — 186004731 186006611

OBD 50 x 50 mm Column 5 µm 186003933 186003934 186003935 186003936 186004732 —

OBD 50 x 100 mm Column 5 µm 186003937 186003938 186003939 186003940 186004733 —

OBD 50 x 150 mm Column 5 µm 186003929 — — — 186004734 —

OBD 50 x 250 mm Column 5 µm 186004107 — — — 186004735 —

10 x 10 mm Guard 10 µm 1860038891 1860040031 1860039881 — — —

10 x 150 mm Column 10 µm 186003890 186004004 186003989 — — —

10 x 250 mm Column 10 µm 186003891 186004005 186003990 — — —

19 x 10 mm Guard 10 µm 1860038922 1860040062 1860039912 — — —

OBD 19 x 50 mm Column 10 µm 186003893 186004007 186003992 — — —

OBD 19 x 100 mm Column 10 µm 186003901 186004008 186003993 — — —

OBD 19 x 150 mm Column 10 µm 186003894 186004009 186003994 — — —

OBD 19 x 250 mm Column 10 µm 186003895 186004010 186003995 — — —

30 x 10 mm Guard 10 µm 1860068923 1860068943 1860068973 — — —

OBD 30 x 75 mm Column 10 µm 186004711 — — — — —

OBD 30 x 100 mm Column 10 µm 186003930 — — — — —

OBD 30 x 150 mm Column 10 µm 186003896 186004011 186003996 — — —

OBD 30 x 250 mm Column 10 µm 186003897 186004012 186003997 — — —

OBD 50 x 50 mm Column 10 µm 186003898 186004013 186003998 — — —

OBD 50 x 100 mm Column 10 µm 186003902 186004014 186003999 — — —

OBD 50 x 150 mm Column 10 µm 186003899 186004015 186004001 — — —

OBD 50 x 250 mm Column 10 µm 186003900 186004016 186004002 — — —1 Requires 10 x 10 mm Prep Guard Holder, p/n: 2890007792 Requires 19 x 10 mm Prep Guard Holder, p/n: 1860007093 Requires 30 x 10 mm Prep Guard Holder, p/n: 186006912

XBridge BEH PhenylPhenyl chromatographic phases have always been of interest to the chromatographer due to the alternative selectivity, particularly with respect to polyaromatic compounds. Unfortunately, routine use of these phases is not common due to their inherent poor stability and reproducibility.

A combination of the second generation HPT and a tri-functional bonding approach in the XBridge BEH Phenyl OBD Preparative Column delivers, for the first time, a truly pH stable and reproducible phase, ideal for drug purification.

XBridge BEH Amide and XBridge BEH HILICHydrophilic-Interaction Chromatography (HILIC) is a technique used to retain and separate very polar compounds that cannot be retained using reversed-phase chromatography. XBridge OBD Preparative HILIC Columns contain the rugged unbonded BEH particle that offers excellent chemical stability for long column lifetimes and is designed for the purification of very polar basic compounds.

XBridge BEH Amide OBD Preparative Columns should be considered for the purification of synthetic saccharides, synthetic sugars, oligosaccharide, glycopeptides, natural products, and synthetic highly polar compounds.




























































































































































Preparative Columns

Maximum Efficiency/30% Lower Backpressure

0 2 4 6

1 2

3

45

12

3

4

5

8 10 12 14 16 18 20 min

XBridge OBD Preparative Columns deliver the same high loading capacity and reliability expected of our XTerra Prep Products, with a significantly reduced column backpressure.

XTerra MS C18 OBD Prep, 5 µm, 19 x 50 mmPart number: 186001930Injection vol.: 660 µLTotal load: 198 mgMaximum backpressure: 1000 psiFlow rate: 23.8 mL/min

XBridge BEH C18 OBD Prep, 5 µm 19 x 50 mmPart number: 186002977Injection vol.: 660 µLTotal load: 198 mgMaximum backpressure: 700 psiFlow rate: 23.8 mL/min

Mobile phase A: 0.1% DEA in waterMobile phase B: 0.1% DEA in acetonitrileSample conc.: 300 mg/mL in DMSOSystem: AutoPurificationDetection: UV @ 260 nm

Compounds:1. Labetolol 50 mg/mL2. Quinine 50 mg/mL3. Diltiazem 50 mg/mL4. Verapamil 100 mg/mL5. Amitriptyline 50 mg/mL

Gradient: Time Profile (min) %A %B 0.00 95 5 1.79 95 5 6.79 5 95 7.79 5 95

Gradient: Time Profile (min) %A %B 0.00 95 5 1.79 95 5 6.79 5 95 7.79 5 95

A Particle Designed for Purification

Preparative packing materials are routinely offered with a much wider particle size distribution than the equivalent analytical particle. While this approach does reduce the cost of material manufacturing, the chromatographic performance is almost certainly compromised.

XBridge OBD preparative packing materials are fully optimized to deliver maximum efficiency and the highest loadability at the lowest possible backpressures. These attributes allow the purification chemist the flexibility to choose the best combination of particle size and column dimension to easily purify even the most complex of samples.

Waters has a wide range of products for peptide purification.

For more information, see page 236.

Did you know...




SUNFIRE OBD PREPARATIVE COLUMNSSunFire Columns set the standard for the state-of-the-art bonded C18 and C8 silica HPLC Columns. Benefiting from years of research and product development, SunFire Columns represent the best in particle and bonding expertise and deliver the industry-leading level of chromatographic performance.

Loadability by Design

The physicochemical characteristics of sorbents used for preparative separations must be designed and/or chosen not only to achieve the selectivity necessary for optimal separations but also the load capacity that enables maximum throughput. This becomes especially tricky for ionizable compounds in mobile phases with low to moderate pH. SunFire sorbents are made from an engineered synthetic silica with proprietary surface-chemistry modifications, all done under cGMP protocols, that extend loadability far beyond that of competitive packings.

Isolation of Nimodipine and Its ImpuritiesHigh Mass Loading of SunFire Sorbents Enables the Use of Smaller Preparative Column Dimensions

1

2

3

1

2

3

4

4

20 4 6 8 10 12 14 min

-1

100

%

-1

100

%

Mobile phase A: Water with 0.1% formic acid Mobile phase B: Acetonitrile with 0.1% formic acidAnalyte: Nimodipine at 30 mg/mL in DMSODetection: UV @ 290 nmSystem: AutoPurification

SunFire C18 OBD PREP, 5 µm,19 x 100 mmPart number: 186002567Total mass load: 102 mg

SunFire C18, 5 µm, 4.6 x 100 mmPart number: 186002558Total mass load: 6 mg

Flow rate: 1.4 mL/minGradient: Time Profile (min) %A %B 0.00 70 30 1.00 70 30 11.00 10 90 12.00 10 90 12.10 70 30 16.00 70 30Injection vol.: 200 µL

Flow rate: 23.9 mL/minGradient: Time Profile (min) %A %B 0.00 70 30 1.97 70 30 11.97 10 90 12.97 10 90 13.10 70 30 16.00 70 30Injection vol.: 3.4 mL

Impurity Peak % by Arealevels: 1 3.1 2 0.6 3 6.5 4 4.9

Rs: 2.77W1/2: 0.17

Rs: 2.31W1/2: 0.21

Rs: 1.92W1/2: 0.26

Rs: 0.81W1/2: 0.90

Rs: 1.01W1/2: 0.64

Rs: 1.19W1/2: 0.53

SunFire C18 Kromasil® C18

Rs: 1.87W1/2: 0.27

Rs: 0.78W1/2: 0.91

250 µg

500 µg

1000 µg

1250 µg

Column: SunFire C18, 5 µm, 4.6 x 150 mmPart number: 186002559Mobile phase A: 0.1% TFA in waterMobile phase B: 0.1% TFA in acetonitrileGradient: 15 min linear gradient from 20% B to 85% B, 2 min initial holding timeFlow rate: 1 mL/minInjection vol.: 10 µL Detection: UV @ 254 nmSystem: Alliance HT 2795

Compounds:1. Ketoconazole2. Econazole3. Miconazole

Sample Concentration: As indicated in the table, dissolved in DMSOAnalyte mg/mL mg/mL mg/mL mg/mL

Ketoconazole 25 20 10 5

Econazole 50 40 20 10

Miconazole 50 40 20 10

Total Concentration 125 100 50 25





Preparative Columns

SunFire Preparative Columns

Dimension TypeParticle

Size C18 C8 Silica Dimension Type

Particle Size

C18 C8 Silica

10 x 10 mm Guard 5 µm 1860025651 1860027501 1860034291 50 x 250 mm Column 5 µm 186003970 — —

10 x 50 mm Column 5 µm 186002561 186002746 186003425 10 x 10 mm Guard 10 µm 1860026631 1860027581 1860034411

10 x 100 mm Column 5 µm 186002562 186002747 186003426 10 x 50 mm Column 10 µm 186003840 186003841 Custom

10 x 150 mm Column 5 µm 186002563 186002748 186003427 10 x 150 mm Column 10 µm 186002664 186002759 186003442


19 x 10 mm Guard 5 µm 1860025692 1860027542 1860034342 19 x 10 mm Guard 10 µm 1860026662 1860027612 1860034442




19 x 250 mm Column 5 µm 186004027 186004028 186004029 30 x 10 mm Guard 10 µm 1860068843 1860068863 1860068883

30 x 10 mm Guard 5 µm 1860068853 1860068873 1860068893 30 x 50 mm Column 10 µm 186003854 186003853 186003855

30 x 50 mm Column 5 µm 186002570 186002755 186003435 30 x 100 mm Column 10 µm 186003971 — —




30 x 250 mm Column 5 µm 186003969 — — 50 x 100 mm Column 10 µm 186003972 — —



50 x 150 mm Column 5 µm 186003941 — — 100 x 250 mm Column 10 µm 186003928 — —1 Requires 10 x 10 mm Cartridge Holder, p/n: 2890007792 Requires 19 x 10 mm Cartridge Holder, p/n: 1860007093 Requires 30 x 10 mm Prep Guard Holder, p/n: 186006912

SunFire Preparative Scouting ColumnsDimension Particle Size C18 C8 Silica

4.6 x 150 mm 5 µm — — 186003453

4.6 x 250 mm 5 µm — — 186003454

4.6 x 150 mm 10 µm 186003390 Custom 186003467

4.6 x 250 mm 10 µm 186003391 Custom 186003468

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Purification of Diesters from Standard DCC/DMAP Protocol*

0 2 4 106 12 min8

12

3 4

O

O

O

O

O

O

O

O

O

O

HO

O

OH

O

O

HO

Compounds:1. Propyl p-phenylacetobenzoate2. Methyl p-phenylacetobenzoate3. Propyl paraben4. Methyl paraben

Column: SunFire Silica OBD Prep, 5 μm, 19 x 150 mmPart number: 186002568Mobile phase: 80/20 hexane/ethyl acetateFlow rate: 18 mL/minTotal load: 100 mg

Normal-Phase SunFire Silica OBD Preparative ColumnsSunFire Silica OBD Preparative Columns allow you to use normal-phase protocols. This gives you a significant selectivity alternative to reversed-phase preparative chromatography on bonded silicas. Analytes that are highly hydrophilic or insoluble/unstable in water are ideal candidates for normal-phase purification. Unlike the more viscous, higher-boiling aqueous-organic mobile phases used in reversed phase, typical normal-phase eluents are lower-viscosity, lower-boiling organic solvents. As a consequence, SunFire silica separations yield several key benefits:

¡ High efficiency (plate counts similar to those of analytical columns)

¡ Excellent scale-up capability

¡ Lower backpressure

¡ Faster analyte recovery (rapid solvent-evaporation rates)

SunFire Prep Columns, available in 5 µm and 10 µm particle sizes, exhibit enhanced column life and stability due to the OBD design, as well as excellent peak shapes and high-mass loading capacity.

Did you know...Recommended Column Cleaning and Regenerating Procedures

See the cleaning routine that matches the properties of the column and what you believe is contaminating it. Flush columns with 20 column volumes of HPLC-grade solvents (e.g. 80 mL total for 4.6 x 250 mm column). Run columns in reverse flow direction, slowly at first, with the outlet disconnected from the detector. Cleaning efficiency is increased by increasing mobile-phase temperature to 35–55 °C. If the column performance is poor after regenerating and cleaning, call Waters Chemistry Technical Support Service for additional assistance. The Preparative Chromatography Mix Standard (p/n: 186006703) is also available to help you benchmark and trend your system performance and cleaning efficiency.

Silica-based particles: Non-polar-bonded phases

Polar Samples Non-Polar Samples Proteinaceous Samples

1. Water 1. Isopropanol Option 1: Inject repeated aliquots of DMSO2. Methanol 2. THF

3. THF 3. Dichloromethane Option 2: Gradient of 10 to 90% B where: A = 0.1% TFA in water B = 0.1% TFA in CH3CN

Option 3: Flush column with 7 M guanidine HCl, or 7 M urea

4. Methanol 4. Hexane

5. Water 5. Isopropanol

6. Mobile phase 6. Mobile phase

Silica-based particles: Polar-bonded phases

Polar Samples (aqueous mobile phases)

Non-Polar Samples (non-aqueous mobile phases)

1. Water 1. Cloroform

2. Methanol 2. Methanol

3. THF 3. Dichloromethane

4. Methanol 4. Heptane or isoctane

5. Water 5. Isopropanol

6. Mobile phase 6. Mobile phase

Polymer-based particles (non-GPC)1. Flush column with mobile phase but omit buffers or salts (for example, just organic and water, acetonitrile is preferable)

2. Run a gradient to 100% organic

3. Flush with 20 column volumes of THF

4. Flush with 20 column volumes of acetonitrile

5. Run a gradient back to starting mobile-phase conditions, omitting buffers and salts

6. Re-equilibrate in mobile phase

*A base-catalyzed (DMAP) esterification reaction, driven to completion using a dehydrating agent (DCC). RCOOH + R'OH ↔ RCOOR' + H20

DMAP = 4-Dimethylaminopyridine; DCC = 1,3-Dicyclohexylcarbodiimide; THF = Tetrahydrofuran

DCC/DMAP in THF

Propyl paraben

HO

O

O

Methyl paraben

HO

O

O

Phenylacetic acidOH

O

Propyl p-phenylacetobenzoate

O

O

O

O

Methyl p-phenylacetobenzoate

O

O

O

O




Preparative Columns

Beta Blockers

0 2 4 6 8 10 12 min

AU

0.0

1.0

2.0

3.0

AU

0.0

1.0

2.0

3.0

AU

0.0

1.0

2.0

3.0

1

2

3

1

2

3

V0 = 1.5 min1

2

3

V0 = 1.5 min

V0 = 1.5 min

Atlantis T3 OBD Prep, 5 µm, 19 x 100 mmPart number: 186003697Flow rate: 17.06 mL/minInjection vol.: 256 µL (112.6 mg total mass load)

Atlantis T3 OBD Prep, 5 µm, 30 x 100 mmPart number: 186003702Flow rate: 42.53 mL/minInjection vol.: 638 µL (280.7 mg total mass load)

Analytical Gradient TableGradient: Time Profile (min) %A %B 0.0 100 0 1.0 100 0 2.0 82 18 6.0 28 72 8.0 28 72 8.1 0 100 10.0 0 100 10.1 100 0 15.0 100 0

Compounds:1. Atenolol 40 mg/mL in DMSO2. Metoprolol 200 mg/mL in DMSO3. Propranolol 200 mg/mL in DMSO

Mobile phase A: 0.1% formic acid in waterMobile phase B: 0.1% formic acid in acetonitrileDetection: UV @ 280 nmSystem: 2525 Binary Gradient Module, 2767 Sample Manager, 2996 PDA (AutoPurification flow cell)Temperature: Ambient

Preparative Gradient TableGradient: Time Profile (min) %A %B 0.0 100 0 2.0 100 0 3.0 82 18 7.0 28 72 9.0 28 72 9.1 0 100 11.0 0 100 11.1 100 0 16.0 100 0

Atlantis T3, 5 µm, 4.6 x 100 mmPart number: 186003746 Flow rate: 1.0 mL/minInjection vol.: 15 µL (6.6 mg total mass load)

ATLANTIS OBD PREPARATIVE COLUMNSFirst introduced in 2002, the Atlantis product line has rapidly gained acceptance as the industry standard for polar compound retention and purification. Available for both reversed-phase and HILIC modes of chromatography, these phases exhibit significantly increased retention characteristics as compared to conventional C18 or embedded polar group column chemistries.

Atlantis dC18

The first in the family to be introduced, Atlantis dC18 is a reversed-phase packing bonded with a difunctional-C18 ligand to an intermediate ligand density (1.6 µmoles/m2) and fully endcapped. For maximum column lifetime, the dC18 phase is recommended for mobile-phase pH values of 2.5–6.

Atlantis T3An evolution of the dC18 product, Atlantis T3 Columns retain all of the benefits of its predecessor but exhibits significantly improved stability in low-pH mobile phases as well as improved peak shape for amine containing bases at pH 7. T3 bonding utilizes a trifunc-tional-C18 ligand (1.6 µmoles/m2) and a proprietary end capping process, which have been proven to be much more effective than the traditional trimethyl silane (TMS) end capping.





Atlantis HILIC SilicaHILIC is a variation of normal-phase chromatography where a polar stationary phase is used with a mobile phase which contains a high concentration of organic (non-polar) solvent and a low concentration of aqueous (polar) solvent. In HILIC, the organic portion of the mobile phase is the weak solvent, the aqueous portion the strong solvent, and the compounds elute in the order of increasing hydrophilicity. Due to the high organic, low viscosity mobile phases that are employed, HILIC provides low column backpressure. Additionally, the collected fractions of interest are inherently high in organic, enabling faster solvent evaporation compared to reversed phase.

Nucleic Acid Bases Scale-up on Atlantis HILIC Columns

1 2 3 4 min

1

2

3

1

2

3

1 2 3 4 min

Atlantis HILIC, 5 µm, 4.6 x 50 mm

Atlantis Silica HILIC OBD Prep, 5 µm, 19 x 50 mm

Columns: Atlantis Silica HILIC, 5 µm, 4.6 x 50 mm Atlantis Silica HILIC Prep OBD, 5 µm, 19 x 50 mmPart numbers: 186002028 (analytical), 186003957 (preparative)Mobile phase A: 10 mM ammonium formate, pH 3 bufferMobile phase B: Acetonitrile/100 mM ammonium formate, pH 3 (90/10) v/vGradients: As indicated to the rightFlow rates: 1.4 mL/min (analytical); 23.9 mL/min (preparative)Sample conc.: 25 µg/mL each in acetonitrile/methanol (75/25) v/vInjection vol.: 60 µL (analytical); 1020 µL (preparative)Weak wash solvent: 95/5 acetonitrile/waterTemperature: AmbientDetection: UV @ 280 nmSystem: AutoPurification with 996 PDA Detector

Void is at 0.55 minutes analytical, 0.56 minutes preparative

Gradient: Time Profile (min) %A %B 0.00 0 100 5.00 10 90 6.00 10 90 6.10 0 100 10.00 0 100Injection vol.: 60 µLTotal loading: 4500 ng

Gradient: Time Profile (min) %A %B 0.00 0 100 0.74 0 100 5.74 10 90 6.74 10 90 6.84 0 100 10.74 0 100 Injection vol.: 1020 µL Total loading: 76.5 µg

Compounds:1. Uracil2. 5-fluorocytosine3. Cytosine

Atlantis Preparative Columns


SizeT3 HILIC dC18 Dimension Type

Particle Size

T3 HILIC dC18

10 x 10 mm Guard 5 µm 1860036951 — 1860023001 10 x 10 mm Guard 10 µm 1860037061 — 1860024521

10 x 50 mm Column 5 µm 186003691 — 186002298 10 x 150 mm Column 10 µm 186003704 — 186002453

10 x 100 mm Column 5 µm 186003692 — 186002299 10 x 250 mm Column 10 µm 186003705 — 186002454

10 x 150 mm Column 5 µm 186003693 — — 19 x 10 mm Guard 10 µm 1860037102 — 1860013632

10 x 250 mm Column 5 µm 186003694 — — OBD 19 x 50 mm Column 10 µm 186003707 — —

19 x 10 mm Guard 5 µm 1860036992 1860039562 1860013612 OBD 19 x 150 mm Column 10 µm 186003708 — 186001369

OBD 19 x 50 mm Column 5 µm 186003696 186003957 186001365 OBD 19 x 250 mm Column 10 µm 186003709 — 186001371

OBD 19 x 100 mm Column 5 µm 186003697 186003958 186001367 30 x 10 mm Guard 10 µm 1860068783 — 1860068753

OBD 19 x 150 mm Column 5 µm 186003698 186003959 186002800 OBD 30 x 75 mm Column 10 µm 186004712 — —

OBD 19 x 250 mm Column 5 µm 186004026 — 186004030 OBD 30 x 150 mm Column 10 µm 186003711 — 186002417

30 x 10 mm Guard 5 µm 1860068793 1860068773 1860068763 OBD 30 x 250 mm Column 10 µm 186003712 — 186002418


OBD 30 x 75 mm Column 5 µm 186003701 — 186002455 OBD 50 x 100 mm Column 10 µm 186004084 — —



OBD 50 x 50 mm Column 5 µm 186004080 — —

OBD 50 x 100 mm Column 5 µm 186004081 — —

OBD 50 x 150 mm Column 5 µm 186004082 — —1 Requires 10 x 10 mm Cartridge Holder, p/n: 2890007792 Requires 19 x 10 mm Cartridge Holder, p/n: 1860007093 Requires 30 x 10 mm Prep Guard Holder, p/n: 186006912






































































Preparative Columns

XTERRA OBD PREPARATIVE COLUMNS

XTerra MS XTerra MS C18 and MS C8 Columns were designed to be compatible with mass spectrometry applications and provide sharp peaks, good sensitivity, and large peak capacities. These chemistries exhibit peak shape usually only achievable with columns employing embedded polar group technology (see XTerra RP Columns). The tri-functional bonding chemistries of XTerra MS Columns deliver the longest lifetimes over the widest pH range (pH 1–12) and provide maximum throughput with excellent resolution and ultra-low bleed.

XTerra RP XTerra RP18 and RP8 Columns combine Hybrid Particle Technology with Shield Technology by incorporating an embedded polar group to deliver the best possible peak shape. These general purpose columns allow fast method development by offering unique selectivity. Like all XTerra Columns, the shielded XTerra RP Columns exhibit excellent water wettability, even in 100% aqueous mobile phases. T he combination of a greatly widened pH range (pH 2–12) for selectivity choices and the additional selectivity options of two ligands are often the only tools necessary for successful method development.

XTerra Preparative ColumnsDimension Type Particle Size MS C18 MS C8 RP18 RP87.8 x 10 mm Cartridge 5 µm 1860011684 1860011694 1860011704 1860011714

7.8 x 50 mm Column 5 µm 186001152 186001153 186001154 186001155

7.8 x 100 mm Column 5 µm 186001156 186001157 186001158 186001159

7.8 x 150 mm Column 5 µm 186001475 186001476 186001477 18600147810 x 10 mm Cartridge 5 µm 1860010011 1860010041 1860010061 1860010081

10 x 30 mm Column 5 µm 186001010 186001011 186001012 18600101310 x 50 mm Column 5 µm 186001014 186001015 186001016 18600101710 x 100 mm Column 5 µm 186001018 186001019 186001020 18600102110 x 150 mm Column 5 µm 186001479 186001480 186001481 18600148219 x 10 mm Cartridge 5 µm 1860011042 1860011052 1860011062 1860011072

OBD 19 x 50 mm Column 5 µm 186001930 186001931 186001932 186001933OBD 19 x 100 mm Column 5 µm 186001934 186001935 186001936 186001937OBD 19 x 150 mm Column 5 µm 186002379 186002380 186002381 18600238230 x 10 mm Guard 5 µm 1860069033 1860069043 1860069063 —OBD 30 x 50 mm Column 5 µm 186001938 186001939 186001940 186001941OBD 30 x 75 mm Column 5 µm 186002387 186002388 186002389 186002390OBD 30 x 100 mm Column 5 µm 186001942 186001943 186001944 186001945OBD 50 x 50 mm Column 5 µm 186002218 186002219 186002220 186002221OBD 50 x 100 mm Column 5 µm 186002222 186002223 186002224 186002225

7.8 x 10 mm Cartridge 10 µm 1860011724 1860011734 1860011744 1860011754

7.8 x 150 mm Column 10 µm 186001160 186001161 186001162 186001163

7.8 x 300 mm Column 10 µm 186001164 186001165 186001166 186001167

10 x 10 mm Cartridge 10 µm 1860010021 1860010051 1860010071 1860010091

10 x 150 mm Column 10 µm 186001022 186001023 186001024 186001025

10 x 250 mm Column 10 µm 186001026 186001027 186001028 186001029

10 x 300 mm Column 10 µm 186001030 186001031 186001032 186001033

19 x 10 mm Cartridge 10 µm 1860010342 1860010352 1860010362 1860010372

OBD 19 x 50 mm Column 10 µm 186002254 — — —

OBD 19 x 150 mm Column 10 µm 186002255 186002256 186002257 186002258

OBD 19 x 250 mm Column 10 µm 186002259 186002260 186002261 186002262

OBD 19 x 300 mm Column 10 µm 186002263 186002264 186002265 186002266

30 x 10 mm Guard 10 µm 1860069023 — 1860069053 —

OBD 30 x 150 mm Column 10 µm 186002267 186002268 186002269 186002270

OBD 30 x 250 mm Column 10 µm 186002271 186002272 186002273 186002274

OBD 30 x 300 mm Column 10 µm 186002275 186002276 186002277 186002278

OBD 50 x 50 mm Column 10 µm 186002279 186002280 186002281 186002282

OBD 50 x 150 mm Column 10 µm 186002843 186002844 186002845 186002846

OBD 50 x 250 mm Column 10 µm 186002847 186002848 186002849 1860028501 Requires 10 x 10 mm Cartridge Holder, p/n: 289000779 3 Requires 30 x 10 mm Prep Guard Holder, p/n: 1860069122 Requires 19 x 10 mm Cartridge Holder, p/n: 186000709 4 Requires 7.8 x 10 mm Cartridge Holder, p/n: 186000708
























































































































































SYMMETRY PREPARATIVE COLUMNSSymmetry Columns provide the highest standard of reproducibility for total confidence in the long-term compliance of your HPLC methods. Symmetry Columns demonstrate excellent peak symmetry for maximum sensitivity and accurate quantitation. Use SymmetryPrep Columns for lead generation and optimization for development of validated assays for stability testing and impurity profiles.

SymmetryPrepSymmetryPrep 7 µm Columns are manufactured for the chemist who needs to purify new chemical entities or isolate impurities or degradants. The SymmetryPrep 7 µm products are a family of high-capacity, high-efficiency, high-performance liquid chromatography (HPLC) columns that have identical selectivity to the Symmetry 3.5 and 5 µm particle size analytical columns. This guarantees direct method scale-up with no further development. SymmetryPrep Columns deliver the advantages of unmatched peak shape and resolution using simple volatile mobile-phase buffers such as TFA and ammonium acetate. All of these features result in a tremendous time reduction in your research and development programs.

SymmetryShieldSymmetryShield Columns with Shield Technology contain a patented chemistry for reversed-phase HPLC that delivers significant changes in selectivity for polar and basic compounds. SymmetryShield Columns deliver unique reversed-phase selectivity with significantly improved peak shape and resolution even for the most challenging analytes.

Symmetry300Symmetry300 Reversed-Phase Columns are designed specifically for high recoveries of peptides and proteins. T hey are the best solution for validation compliance regarding identity, purity, and stability.

SymmetryPrep, SymmetryShield, and Symmetry300 Columns for Purification and IsolationDimension Type Particle Size SymmetryPrep C18 SymmetryPrep C8 SymmetryShield RP18 SymmetryShield RP8 Symmetry300 C18

7.8 x 10 mm Cartridge 5 µm 1860007111 1860007121 — — —

7.8 x 50 mm Column 5 µm 186000208 186000214 — — —

7.8 x 100 mm Column 5 µm 186000209 186000215 — — —

19 x 10 mm Cartridge 5 µm 1860007152 1860007162 1860018352 1860018412 1860018472

19 x 50 mm Column 5 µm 186000210 186000216 186001836 186001842 186001848

19 x 100 mm Column 5 µm 186000211 186000229 186001837 186001843 186001849

19 x 150 mm Column 5 µm — — 186001838 186001844 186001850

30 x 50 mm Column 5 µm 186000235 186000237 — — —

30 x 100 mm Column 5 µm 186000236 186000238 — — —

7.8 x 10 mm Cartridge 7 µm 1860007131 1860007141 — — —

7.8 x 150 mm Column 7 µm WAT066288 WAT066285 — — —

7.8 x 300 mm Column 7 µm WAT066235 WAT066225 — — —

19 x 10 mm Cartridge 7 µm 1860007172 1860007182 — — —

19 x 150 mm Column 7 µm WAT066240 WAT066228 186001839 186001845 —

19 x 300 mm Column 7 µm WAT066245 WAT066230 186001840 186001846 —1 Requires 7.8 x 10 mm Cartridge Holder, p/n: 1860007082 Requires 19 x 10 mm Cartridge Holder, p/n: 186000709Symmetry300 is also available as a C4 chemistry and in a 7 µm particle size. Please inquire.































http://www.waters.com/waters/partDetail.htm?partNumber=WAT066288

















Preparative Columns

µBONDAPAK/BONDAPAK AND µPORASIL/PORASIL COLUMNSThe popular µBondapak C18 chemistry and µPorasil silica packing materials are offered in 10 µm particle size. Bondapak and Porasil are available in two different particle sizes, 15–20, and 37–55 μm, providing easy transfer of chromatography methodology from one particle size to another. You can utilize your existing 10 μm µBondapak or µPorasil chromatography as a starting point for scale-up or, if you are developing a new purification protocol, investigate which preparative particle size provides the best balance between resolution, throughput, and cost.

The preparative Bondapak HC18 HA (high-carbon load, high-activity silica) is a highly carbon-loaded packing that provides different selectivity over the standard Bondapak packing materials. The higher carbon load on the silica surface typically results in a higher loading capability. Bondapak HC18 HA is available in a 37–55 μm particle size.

The Preparative family of packing materials is based on a 55–105 μm Porasil silica and provides a cost effective means for scale-up of preparative processes. The selectivity of Prep silica is identical to 10, 15–20, and 37–55 μm Porasil, while Prep C18 has a slightly different selectivity than Bondapak packings.

µBondapak/Bondapak Preparative ColumnsDimension Type Particle Size Pore Size C18 CN NH2 Phenyl

7.8 x 300 mm Column 10 µm 125Å WAT084176 WAT084177 WAT084178 WAT084179

19 x 150 mm Column 10 µm 125Å WAT088500 — — —

19 x 300 mm Column 10 µm 125Å WAT025828 — — —

7.8 x 300 mm Column 15–20 µm 125Å WAT025832 — — —

19 x 300 mm Column 15–20 µm 125Å WAT025834 — — —

µPorasil/Porasil and Preparative ColumnsDimension Type Particle Size Pore Size µPorasil Porasil

7.8 x 300 mm Column 10 µm 125Å WAT084175 —

19 x 150 mm Column 10 µm 125Å WAT091648 —


3.9 x 300 mm Column 15–20 µm 125Å — WAT025874

19 x 300 mm Column 15–20 µm 125Å — WAT025835

Reversed-Phase Bulk PackingsDescription Particle Size Pore Size Qty. Part No.

Bondapak C18 15–20 µm 125Å 100 g WAT020739

Bondapak C18 15–20 µm 125Å 1 kg WAT020740


Bondapak C18 37–55 µm 125Å 100 g WAT030632



Bondapak HC18 HA 37–55 µm 125Å 100 g WAT035672

Bondapak HC18 HA 37–55 µm 125Å 1 kg WAT035674

Bondapak HC18 HA 37–55 µm 125Å 5 kg WAT035676

Prep C18 55–105 µm 125Å 100 g WAT020594

Prep C18 55–105 µm 125Å 1 kg WAT010001

Prep C18 55–105 µm 125Å 5 kg WAT020595

Prep C18 55–105 µm 125Å 25 kg WAT020596




























Waters Spherisorb Semiprep Columns


SizeODS1 ODS2 C8 C6 C1 NH2 Phenyl

CN Normal Phase

W Silica SAX SCX

10 x 250 mm Column 5 µm PSS830685 PSS831985 PSS831885 PSS831085 PSS832685 PSS831185 PSS830885 PSS830985 PSS830185 PSS832785 PSS837585




µBondapak/Bondapak Radial Compression Column Segments and PrepPak™ Cartridges*Dimension Type Particle Size Pore Size µBondapak C18 µBondapak NH2 µBondapak Phenyl Bondapak C18 Bondapak HC18 HA

8 x 100 mm Column 10 µm 125Å WAT085721 WAT085724 WAT085722 — —

8 x 100 mm Column 15–20 µm 125Å — — — WAT025841 —

8 x 100 mm Column 37–55 µm 125Å — — — — WAT036561

25 x 10 mm Guard-Pak Inserts, 2/pk 10 µm 125Å WAT038518 — — — —

25 x 10 mm Guard-Pak Inserts, 2/pk 15–20 µm 125Å — — — WAT038514 —

25 x 100 mm Column 10 µm 125Å WAT038505 — — — —



25 x 100 mm Column 37–55 µm 125Å — — — — WAT056970

40 x 10 mm Guard-Pak Inserts, 2/pk 10 µm 125Å WAT037839 — — — —

40 x 10 mm Guard-Pak Inserts, 2/pk 15–20 µm 125Å — — — WAT037833 —

40 x 100 mm Column 10 µm 125Å WAT037684 — — — —



*All column segments and cartridges require the appropriate holder/module, see page 221.

µPorasil/Porasil Radial Compression Column Segments and PrepPak Cartridges*Dimension Type Particle Size Pore Size µPorasil Porasil


25 x 10 mm Guard-Pak Inserts, 2/pk 10 µm 125Å WAT038516 —

25 x 10 mm Guard-Pak Inserts, 2/pk 15–20 µm 125Å — WAT038512


25 x 100 mm Column 15–20 µm 125Å — WAT038502

40 x 10 mm Guard-Pak Inserts, 2/pk 10 µm 125Å WAT037836 —

40 x 10 mm Guard-Pak Inserts, 2/pk 15–20 µm 125Å — WAT037830


40 x 100 mm Column 15–20 µm 125Å — WAT037672*All column segments and cartridges require the appropriate holder/module, see page 221.

WATERS SPHERISORB COLUMNSWaters Spherisorb Columns are the most widely referenced HPLC Columns in scientific literature. There are over 2,000 abstracts published on Spherisorb Columns, providing a tremendous range of validated methods and applications to assist you in your method development process.

http://www.waters.com/waters/partDetail.htm?partNumber=PSS830685






































































Preparative Columns

PREP NOVA-PAK HR COLUMNSPrep Nova-Pak HR 6 µm ultra high-efficiency packing materials can be used in shorter columns to obtain the same resolution as longer columns with larger particle size packing materials. Shorter columns mean faster separation, lower solvent consumption and more concentrated fractions. Prep Nova-Pak HR has the same selectivity and retention characteristics as the analytical Nova-Pak 4 µm material. The Prep Nova-Pak HR packing materials are ideal for the separation of a wide range of compounds such as organic synthesis intermediates or natural products.

Prep Nova-Pak HR Columns

Description Type Particle Size Pore Size Dimension Part No.

Prep Nova-Pak HR C18 Column 6 µm 60Å 3.9 x 300 mm WAT038500

Prep Nova-Pak HR C18 Column 6 µm 60Å 7.8 x 300 mm WAT025820

Prep Nova-Pak HR C18 Column 6 µm 60Å 19 x 300 mm WAT025822

Prep Nova-Pak HR Silica Column 6 µm 60Å 3.9 x 300 mm WAT038501

Prep Nova-Pak HR Silica Column 6 µm 60Å 7.8 x 300 mm WAT025821

Prep Nova-Pak HR Silica Column 6 µm 60Å 19 x 300 mm WAT025823

PREPARATIVE BULK MATERIALWaters offers a number of different bulk packing materials for lab-to-process-scale purifications. All our packing materials are manufactured under cGMP (current Good Manufacturing Practices) ensuring a reproducible material for long-term reproducibility. Our manufacturing facilities are ISO 9001-certified. Bulk materials are available packaged in quantities of 100 g to 5 kg. For larger quantity purchases, please inquire about pricing and availability.

All bulk packings are carefully quality controlled using a wide array of physical and chemical tests to ensure consistent batch-to-batch reproducibility.

Normal-Phase Bulk PackingsDescription Particle Size Pore Size Qty. Part No.

Porasil Silica 15–20 µm 125Å 100 g WAT020731

Porasil Silica 15–20 µm 125Å 1 kg WAT020732



Porasil Silica 37–55 µm 125Å 100 g WAT020721




Prep Silica 55–105 µm 125Å 100 g WAT020587

Prep Silica 55–105 µm 125Å 1 kg WAT010004






















DELTA-PAK COLUMNSDelta-Pak packing materials are ideal for the separation of peptides, proteins, and natural products. The isolation and purification of a peptide is usually a multi-step procedure with the fractions from the first run being re-chromatographed on the same preparative column to obtain pure product. Delta-Pak packing materials are based on a highly stable, bonded, endcapped 5 and 15 µm packing. The 5 µm packing is available in analytical-scale dimensions for preliminary preparative chromatographic studies, peptide mapping, and fraction purity assays. The chemistry characteristics of the packing materials are independent of the particle size.

Delta-Pak Preparative ColumnsDimension Type Particle Size Pore Size Delta-Pak C18 Delta-Pak C4

3.9 x 300 mm Column 15 µm 100Å WAT011797 WAT011807




19 x 300 mm Column 15 µm 100Å WAT011799 WAT011809





Delta-Pak Radial Compression Column Segments and PrepPak Cartridges*Dimension Type Particle Size Pore Size Delta-Pak C18 Delta-Pak C4





25 x 10 mm Guard, 2/pk 15 µm 100Å WAT038520 WAT038524

25 x 10 mm Guard, 2/pk 15 µm 300Å WAT038522 WAT038526



40 x 10 mm Guard, 2/pk 15 µm 100Å WAT037842 —

40 x 10 mm Guard, 2/pk 15 µm 300Å WAT037845 WAT037851*All column segments and cartridges require the appropriate holder/module, see page 221.

Did you know...Waters offers a wide range of applications to help you with your analyses.

Search by compound name, family, or class of compound at www.waters.com/applications





































Preparative Columns

PrepLC 25 mm ModulePrepLC 25 mm Module for 25 x 100 mm column segments

PrepLC AssembliesPrepLC Assembly for 40 x 100 mm and 25 x 100 mm column segments

PrepLC Assembly* 25 x 100 mm

PrepLC Assembly* 40 x 100 mm

8 x10 Holder8 x 10 Holder for 8 x 100 mm and 5 x 100 mm Radial-Pak Column segments

8 x10 HolderDimension Part No.

8 x 10 Holder WAT082887

8 x 10 Extension Kit (Includes 1 Extension Tube, Union, O-rings) WAT038846

Cartridge Union WAT038849

PrepLC 25 mm ModuleDescription Part No.

PrepLC 25 mm Module WAT015814

PrepLC 25 mm Extension Kit (Includes 1 Extension Tube, Union, O-rings) WAT022180

Extension Tube WAT019311

PrepLC AssembliesDescription Part No.

PrepLC 40 mm Assembly (Includes PrepLC Universal Base and PrepLC 40 mm Chamber) WAT022441

PrepLC Universal Base WAT027577

PrepLC 40 mm Chamber (Includes O-rings, Spacer, and Union) WAT027578

PrepLC 40 mm Extension Kit (Includes Extension Tube, Union, and O-rings) WAT022365

PrepLC 25 mm Chamber (Includes O-rings, Spacer, and Union) WAT033994

PrepLC 25 mm Extension Kit (Includes 1 Extension Tube, Union, and O-rings) WAT022180

PrepLC Scale-up Kit with Capability for 40 mm or 25 x 300 Length WAT022440

Includes: 1 - PrepLC Universal Base

2 - PrepLC Chambers (One Each of 40 mm and 25 mm)

2 - PrepLC 25 mm Extension Kits

2 - PrepLC 40 mm Extension Kits

RADIAL COMPRESSION MODULESWe carry a complete inventory of accessories and spare parts for the Waters patented Radial Compression Modules for use with the 5 mm and 8 mm I.D. Radial-Pak Column segments, the 25 mm and 40 mm I.D. PrepLC™ Column segments, and the 47 mm I.D. PrepPak Cartridges.















PREPPAK CARTRIDGESRequires PrepPak 1000 Module (p/n: WAT089592).

PURIFICATION AND ISOLATION CARTRIDGE HOLDERS

ACCESSORIES AND SPARE PARTS FOR WATERS RADIAL COMPRESSION CARTRIDGE HOLDERS AND MODULESWe carry a complete inventory of accessories and spare parts for Waters patented Radial Compression Modules for use with the 5 mm and 8 mm I.D. x 100 mm long Radial-Pak Column segments, 25 mm and 40 mm I.D. x 100 mm long PrepLC Column segments, and 47 mm I.D. x 300 mm long PrepPak Cartridges.

30 x 10 mm Prep Guard Holder and Cartridge

Purification and Isolation Cartridge HoldersDescription Part No.

7.8 x 10 mm Cartridge Holder 186000708

10 x 10 mm Cartridge Holder 289000779

19 x 10 mm Cartridge Holder 186000709

30 x 10 mm Prep Guard Holder 186006912

Replacement O-ring 7.8 mm, 2/pk 700001019

Replacement O-ring 10 mm, 2/pk 700001436



PrepPak Cartridges*(Requires PrepPak 1000 Module, p/n: WAT089592)Description Particle Size Pore Size Dimension Part No.

Bondapak C18 15–20 µm 125Å 47 x 300 mm WAT091784

Bondapak C18 15–20 µm 300Å 47 x 300 mm WAT038571

Bondapak HC18 HA 37–55 µm 125Å 47 x 300 mm WAT038570

Bondapak NH2A 55–105 µm 125Å 47 x 300 mm WAT091631

Delta-Pak C18 15 µm 100Å 47 x 300 mm WAT015401




Prep C18 55–105 µm 125Å 47 x 300 mm WAT025876

Porasil Silica (single) 37–55 µm 125Å 47 x 300 mm WAT025853

Porasil Silica (10/case) 37–55 µm 125Å 47 x 300 mm WAT025877

PrepPak 1000 Module for 47 x 300 mm PrepPak Cartridges WAT089592*All column segments and cartridges require the appropriate holder/module, see page 221.

PrepLC Spare PartsColumn Part No.

PrepLC Universal Base Spare Parts

O-ring Removal Tool WAT082853

O-ring for Pressure Piston WAT022281

O-ring for Filling Port WAT005129

Filling Port Plug WAT027509

Ferrules and Compression Fittings (stainless steel), 5/pk WAT025604

PrepLC 40 mm Chamber Spare Parts

Column Segment Union WAT033996

Cartridge Spacer WAT033997

O-ring, Base Plate (small) WAT022453

O-ring, Base Plate (large) WAT022454

O-ring, Chamber Top WAT022280

O-ring (Normal-phase) Cartridge, Top and Bottom, Spacers, and Unions WAT027519

O-ring (Normal-phase) Chamber, Bottom WAT022299

O-ring (Normal-phase) Inner Connector, Top and Bottom WAT022297

O-ring (Reversed-phase) Cartridge, Top and Bottom, Spacers, and Unions WAT027518

O-ring (Reversed-phase) Chamber, Bottom WAT022283

O-ring (Reversed-phase) Inner Connector, Top and Bottom WAT015835

O-ring, Extension Tube WAT022454

PrepLC 25 mm Chamber Spare Parts


Segment Spacer WAT015859

O-ring, Base Plate (small) WAT022276

O-ring, Base Plate (large) WAT015831

O-ring, Chamber Top WAT015833

O-ring (Normal-phase) Cartridge Top and Bottom, Spacers, and Unions WAT015848

O-ring (Normal-phase) Chamber Bottom WAT022298

O-ring (Normal-phase) Inner Connector, Top and Bottom WAT022297

O-ring (Reversed-phase) Cartridge, Top and Bottom, Spacers, and Union WAT015834

O-ring (Reversed-phase) Chamber Bottom WAT022282

O-ring (Reversed-phase) Inner Connector, Top and Bottom WAT015835

Tubing Fluid Path Kit* (PEEK) (Includes Inner Connectors, Tubing, Ferrules, and Compression Screws)

WAT022400

*For applications where a metal-free flow path is needed

19 x 10 mm Prep Guard Holder and Cartridge





















































Preparative Columns

Nova-Pak and Prep Nova-Pak Radial Compression Column Segments and PrepPak CartridgesDescription Particle Size Pore Size Dimension Part No.

Nova-Pak Radial-Pak Column Segments

Nova-Pak C18 4 µm 60Å 5 x 100 mm WAT080100

8 x 100 mm WAT086342

Nova-Pak C8 4 µm 60Å 5 x 100 mm WAT035890

8 x 100 mm WAT035884

Nova-Pak Phenyl 4 µm 60Å 5 x 100 mm WAT010657

8 x 100 mm WAT010658

Nova-Pak CN HP 4 µm 60Å 5 x 100 mm WAT010224

8 x 100 mm WAT010223

Nova-Pak Silica 4 µm 60Å 5 x 100 mm WAT010986

8 x 100 mm WAT010987

Prep Nova-Pak HR Radial-Pak Column Segments

Prep Nova-Pak HR C18 6 µm 60Å 8 x 100 mm WAT025843

Prep Nova-Pak HR Silica 6 µm 60Å 8 x 100 mm WAT025844

Prep Nova-Pak HR PrepLC 25 mm Column Segments



Prep Nova-Pak HR 25 x 10 Guard-Pak Inserts, 2/pk



Prep Nova-Pak HR PrepLC 40 mm Column Segments



Prep Nova-Pak HR 40 x 10 Guard-Pak Inserts, 2/pk



Resolve Radial Compression Column Segments and PrepPak Cartridges*Dimension Particle Size Pore Size C18 C8 Silica CN

8 x 100 mm 5 µm 90Å WAT0846241 — WAT084634 —

5 x 100 mm 10 µm 90Å WAT084620 WAT085672 WAT084630 WAT084626

8 x 100 mm 10 µm 90Å WAT084720 WAT085670 WAT084730 WAT0846361 Requires 8 x 100 cartridge holder, p/n: WAT082887* Recommended for Lanthanide Analysis, see page 196.

8 x10 Cartridge Holder, Parts and AccessoriesDimension Part No.

8 x 10 Holder WAT082887

8 x 10 Extension Kit (Includes 1 Extension Tube, Union, O-rings) WAT038846


O-ring for Extension Tube WAT038851

Connector Tubing Assembly (Non-metallic) WAT088919

Connector Assembly (Stainless Steel) WAT082892

Washer for Connectors, 10/pk WAT005147

Pressure Relief Plug WAT088027

Check Valve WAT082888

O-ring (Large) for Connector, 10/pk WAT005130

O-ring (Small) for Connector (Normal-phase), 4/pk WAT015797

O-ring (Small) for Connector (Reversed-phase), 10/pk WAT005129

O-ring for Filling Port, 10/pk WAT005129


Gripper Ring Replacement Kit (Includes 10 Gripper Rings, 20 Washers, 10 Ferrules, and Tool)

WAT021908

PrepLC 25 mm Module, Parts and AccessoriesDimension Part No.

PrepLC 25 mm Module WAT015814

PrepLC 25 mm Extension Kit (Includes 1 Extension Tube, Union, O-rings)

WAT022180

Extension Tube WAT019311

O-ring for Extension Tube WAT015831

O-ring (Large) for Connector WAT015833

O-ring (Small) for Connector (Normal-phase) WAT015848

O-ring (Small) for Connector (Reversed-phase) WAT015834

O-ring for Filling Port, 10/pk WAT005129


Union Coupling Assembly WAT015860

Union, 1/8 to 1/16” Tubing, 5/pk WAT005137


























































LITERATURE REFERENCESTitle Literature Code

Control Chart W hite Paper 720004535EN

Troubleshooting Common System Problems Using QCRM 720004635EN


Quality Control Reference Materials (QCRM)Product Name Intended Use Chromatographic Mode Systems Contents Part No.

Preparative Chromatography Mix Standard

Provides chromatographic performance information inclusive of mobile-phase pH using 1 void marker, 1 acidic, 1 basic, and 1 neutral probes.

Reversed-phase All Purification Systems

5 mg/mL each:

¡ Diclofenac sodium salt ¡ Diphenhydramine hydrochloride ¡ Flavone

In a 1 mL solution of DMSO. Store at room temperature.

186006703

AutoPurification System Standard

Tests the performance of fraction collectors, both UV and MS directed, using 3 dyes.

Reversed-phase All Purification Systems with Fraction Collectors

3 ampoules of test mix containing:

¡ 2500 μg/mL thionin ¡ 3000 μg/mL thioflavin ¡ 2500 μg/mL crystal violet

In a 10 mL solution of 25/75 water/methanol. Store at room temperature.

716000765

Search by Literature Code at www.waters.com

HOW DO YOU KNOW YOUR CHROMATOGRAPHIC SYSTEM IS IN PROPER WORKING ORDER?Quality Control Reference Materials (QCRMs) contain mixtures of standards specifically chosen to provide an easy and reliable way to monitor the performance of any chromatographic system. Using a QCRM, you can be assured that your column and system are ready to analyze your samples. Regular use of QCRMs also provides an opportunity to benchmark your chromatographic systems and trend performance over time, making it easier to proactively identify problems and resolve them faster.

Since chromatographic analyses are complex and depend on many different variables, such as mobile-phase composition, column type and detection method, Waters has formulated specific QCRM mixtures designed to test systems with these differences in mind.

To locate additional information for standards specific to calibration, qualification and tuning of instruments and detectors, as well as a more comprehensive listing of available standards and reagents, please see the Analaytical Standards and Reagents e-Catalog at asr.waters.com



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