presentation: 1. bioen research program 2. sugarcane
TRANSCRIPT
Presentation:
1. BIOEN research program
2. Sugarcane genome sequence
• M. A. Van Sluys
• Botânica – IB – USP
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Non-Renewable Renewable
En
erg
y s
ou
rces i
n B
razil,
2006
Brazil: in 2009 47% energy fromrenewable sources; sugarcane 18%
2
cane
18%
Brasil: 47%; Mundo: 13%; OECD: 7,2%
Foto Capa: Leo Ramos
Energy Security
Sugarcane bioethanol contributes to 20% of
the Brazilian liquid fuels matrix
Biomass cogeneration can contribute with up to 18% of Brazil’s electricity demand
Sustainable Development
The sugarcane industry contributes
to agriculture modernization, rural
development, improved education and the creation of
jobs
Opportunities for innovation
Environmental Security
The use of Sugarcane bioethanol can reduce CO2 emissions by 80%
when compared to gasoline
Biofuel certification can contribute to the
reinforcement of agroecological zoning
Food Security
Sugarcane production for energy did no
decrease food production
Expansion is occuring mainly in pasture land
Only 0.5% of brazilian land used to produce
bioethanol
BIOENERGY DRIVERS
BIOEN DIVISIONS
BIOMASSContribute with knowledge and technologies for Sugarcane ImprovementEnable a Systems Biology approach for Biofuel Crops
BIOFUEL TECHNOLOGIESIncreasing productivity (amount of ethanol by sugarcane ton), energysaving, water saving and minimizing environmental impacts
ENGINESFlex-fuel engines with increased performance, durability and decreased consumption, pollutant emissions
BIOREFINERIESComplete substitution of fossil fuel derived compoundsSugarchemistry for intermediate chemical production and alcoholchemistry as a petrochemistry substitute
SUSTAINABILITY AND IMPACTSStudies to consolidate sugarcane ethanol as the leading technology path to ethanol and derivatives productionHorizontal themes: Social and Economic Impacts, Environmental studies and Land Use
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BIOEN Program : 5 Divisions
Fundamental knowledge & New technologies
for a bio-based society
Academic Basic and Applied Research (US$ 40 million)
Since 2008, 136 grants, 400 brazilian researchers, collaborators
from 15 countries
Regular, Theme and Young Investigator Awards
Open to foreign scientists who want to come to Brazil
State of São Paulo Bioenergy Research Center (US$ 90 million)
FAPESP, USP, UNICAMP, UNESP, State of São Paulo
Government (80 new faculty positions for bioenergy
researchers)
Creation of a Bioenergy PhD Program
Partnerships
United States, United Kingdom and The Netherlands, Brazil
Oak Ridge National Laboratories, UKRC, BBSRC, BE-Basic,
GSB, LACAF, BOEING, BP, Braskem, Dedini, ETH, Microsoft,
Oxiteno, PSA Peugeot Citroën, Vale
Innovation Technology, Joint Industry-University research (5 years)
Innovation Centers, Joint Industry-University centers (10 years)
Company Subject
Oxiteno Lignocellulosic materials
Braskem Alcohol-chemistry
Dedini Processes
ETH Agricultural practices
Microsoft Computational development
Vale Ethanol technologies
Boeing Aviation Biofuels
BP Processes and sustainability
PSA Engines
FAPESP Bioenergy Research Program BIOEN
AustraliaAustriaBelgium ChinaDenmarkFinlandFranceGermanyGuatemalaItalyPortugalSpainThe NetherlandsUnited KingdomUnited States 5
15 PITEs and 12 PIPEs
Biomass Research
Precision agricultureTractorsPrediction of sugarcane yieldPest managementAssembly of the sugarcane polyploid genome
Biofuel Technologies
New Saccharomyces cerevisiae strains from the Brazilian biodiversityGrinding processBagasse delignificationBagasse hydrolysisHigh performance fermentation
Biorefineries
Nanofibers from renewable sourcesBiodegradable polymersAcrylic and propionic acid productionGlycerol transformationGlycerol hydrogenolysisGlycerinLactic acidSyngasRubber
Engines
Flex-fuel enginesEthanol spray in internal combustion engines
Sustainability
Atmospheric CO2 mitigationAgricultural GHG emissionsWaste machinesWaste germination and plantation tubes
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SCOPE BIOENERGY & SUSTAINABILITY
Land UseFeedstocks Technologies Impacts
Policy recommendationsMuch Needed Science
Bioenergy numbers
Land use changesBiomass potentialBiomass supplyLogisticsCertificationGHG emissionsLignocellulosicsCosts FinancingSocial aspectsSoil and waterBiodiversityRural developmentEnergy Access
Global assessment of Bioenergy & Sustainability: FAPESP BIOEN, BIOTA and Climate Change Programs in collaboration with SCOPE
International Workshop: December 2-6, 2013, UNESCO, ParisIUPAP Energy Committee
Food Security
Energy Security
Environmental Security and Climate Security
Sustainable Development and Innovation
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SUCESTGenes
ADRESSING THE SUGARCANE GENOME
Markers
Allelic variation for breeding
BIOMASSContribute with knowledge and technologies for Sugarcane ImprovementEnable a Systems Biology approach for Biofuel Crops
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sugarcane polyploid genome
Structural regions (centromere-telomeres)Euchromatin & Heterochromatin
GenesRegulatory regionsRegulatory sequences
Repetitive sequences (Transposable elements, SSR)Translational machinery (ribossomal genes, tRNAs)
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317 BAC sequenced 1,400 protein-coding genes
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Gene evolution
RPA1a
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Gene regulation
RPA1a
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Starch & Sucrose metabolism
EC 5.4.2.2(15413 reads)
EC 2.7.7.9(2358 reads)
EC 2.7.7.273 loci1. Sb09g029610 (1295-5065 reads)
2. Sb01g008940 (443-501 reads)3. Sb03g028850 (4-79 reads)
UDP-glucose
SUCROSE
ADP-glucose
STARCH
maltose
β-D-fructose
α-D-gluocse-6P
Sucrose - 6P
β-D-glucose
AmyloseSh2Cell Wall
components
D - xylose Pectate
D - galacturonate
EC 2.4.1.21(547 reads)
EC 2.4.1.18(3625 reads)
cellulose
EC 2.4.1.12(6237 reads)
EC 2.4.1.13(1698 reads)
EC 2.4.1.14(174-341 reads)
EC 3.1.3.24(109 reads)
EC 3.2.1.2(311 reads)
EC 3.2.1.37(1894 reads)
EC 3.1.1.11(24 reads)
EC 3.2.1.15(23 reads)
EC 3.2.1.26(2 reads)
α-D-gluocse-1P
SUCROSE
STARCH
CELL WALL PARTS
CELLULOSE
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sugarcane hybrid (11)
S. spontaneum (2)
S. offcinarum (1)
Miscanthus sp. (1)
S. bicolor (genome)
single sequence
one substitution
3K2K 4K 5K
SHCRBa_104_G22
1K0 6K
S6PP S6PP
2995F 4500R
4231R
3404F 4144R
3618R
3223F
3811F
Gene innovation
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Initiatives around the globe:SUGESI (Sugarcane Genome Sequencing Initiative) > Strategies
for Sequencing a Highly Complex Genome
1,2,3,5,6,7,8 2,6
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1,4
1,4
1,2,3,7,8
1. BAC-sequencing2. Shotgun sequencing3. Methyl-filtration-NGS4. 11,000 tilling BAC seq5. Sequenom-SNP6. Transcriptome initiatives7. Spont & Off shotgun seq8. Assembly algorithms
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miRNA precursormapping
pathogen treatmentRNAseq mapping
Metilfiltrated gDNA
Other cultivarsgDNA
Integrating DATA around the globe: Euchromatic BAC
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Van Sluys FW Munich 2014
GaTE Lab
Cushla Metcalfe (PD FAPESP)Guilherme M. Q. Cruz (DD FAPESP)Edgar Andres Ochoa (DD CNPq)Andreia Prata (DD FAPESP)Tatiane Correa (TS-USP)Jonas Gaiarsa (DD-FAPESP) – Infra Bioinf
BrasilKatia C Scortecci (UFRN)Douglas da Silva Domingues (IAPAR)
BIOEN
Glaucia M Souza (BIOEN Pgm Coord)
Marcos Buckeridge (INCT coord)Renato Vicentini (CBMEG- UNICAMP)Fabio Nogueira (IB-UNESP Botucatu)Michel Vincentz (CBMEG- UNICAMP)Anete Prereira de Souza (CBMEG- UNICAMP)Monalisa Sampaio (UFSCar – Breeding Pgm)Antonio Augusto Garcia (ESALQ)Helaine Carrer (ESALQ)Maria Lucia Carneiro (ESALQ)Daniel Scherer Moura (ESALQ)Marcio Castro Silva Filho (ESALQ)
Claudia Monteiro-Vitorello (ESALQ - Bioinf)Joao Paulo Kitajima (IEAE-SP – Bioinf)Nathalia de Setta (UFABC)
Team
Partners
InternationalAngelique D’Hont (CIRAD, Fr)Helene Bergers (CNRGV, Fr)Ray Ming (U. Illinois, US)A Paterson (U Georgia, US)K Aitken (CSIRO-Aus)
Financial Support
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The present release of sugarcane genomic sequences will advance our understanding of sugarcane genetics and contribute to the development of molecular tools for breeding purposes and gene discovery. In paralell, tools to assemble the sugarcane genome are beingdeveloped.
Thank you!18