presentation: avocado genetics and breeding - present and … · 2004-09-19 · 1 avocado genetics...
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AVOCADO GENETICS AND AVOCADO GENETICS AND BREEDING BREEDING –– PRESENT PRESENT
AND FUTUREAND FUTURE
U. Lavi,U. Lavi, D. D. Sa'adaSa'ada, I. , I. RegevRegev and E. Lahavand E. Lahav AROARO-- Volcani Center P. O. B. 6, Volcani Center P. O. B. 6,
Bet Bet -- DaganDagan 50250, Israel50250, Israel
Presented at World Avocado Congress V
Malaga, Spain19-24 October, 2003
Abstract A-42
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Why AvocadoWhy Avocado??
A very unique fruitA very unique fruitA relative newcomer to the international A relative newcomer to the international
commercecommerceA very difficult crop to breedA very difficult crop to breed
If you need justifications for being interested in avocado…
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Due toDue to::
Long juvenile periodLong juvenile periodLarge tree sizeLarge tree sizeHigh level of High level of HeterozygosityHeterozygosityNo controlled crosses No controlled crosses Limited genetic knowledgeLimited genetic knowledge
There are various reasons why avocados are considered difficult in terms of breeding.
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Avocado has been consumed in Mexico for 10,000Avocado has been consumed in Mexico for 10,000yearsyears
It was selected mainly for increasing fruit sizeIt was selected mainly for increasing fruit size
The subgenus The subgenus PerseaPersea consists of three speciesconsists of three species : :schiedeanaschiedeana, , parvifoliaparvifolia and and americanaamericana
P. P. americanaamericana consists of the three races: West Indianconsists of the three races: West Indian GuatemalanGuatemalan
MexicanMexican
History and TaxonomyHistory and Taxonomy
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The Main Advantage of Avocado The Main Advantage of Avocado for Breeding Purposes isfor Breeding Purposes is::
The very rich and diverse geneThe very rich and diverse gene--poolpool
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A fruit with a large seed.
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A fruit with a small seed.
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A chimeric fruit
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There is genetic variation in avocado such as fruits with long necks and various colors.
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Classical breeding is mainly based on the amount of genetic variation. This variation is demonstrated in mango fruits.
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A.A. Specific traitsSpecific traits
The genetics of fruitThe genetics of fruit--skin color, flowering group and skin color, flowering group and anise scent was found to be controlled by several anise scent was found to be controlled by several genes and several alleles in eachgenes and several alleles in each..
Various phenotypes result from various heterozygote Various phenotypes result from various heterozygote combinationscombinations
The relevance to breeding projectsThe relevance to breeding projects
Genetic AnalysisGenetic Analysis
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B. Quantitative Analysis: Variance ComponentsB. Quantitative Analysis: Variance Components
High level of High level of heterozygosityheterozygosity (demonstrated by(demonstrated byheterogeneity of avocado seedlings)heterogeneity of avocado seedlings)
Estimated by DNA markersEstimated by DNA markers
The The heterozygosityheterozygosity explains the high estimateexplains the high estimateof nonof non--additive genetic varianceadditive genetic variance..
Genetic AnalysisGenetic Analysis
The variance components, of most avocado traits, are non-additive.
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Is it Necessary to Perform Controlled Is it Necessary to Perform Controlled Crosses for BreedingCrosses for Breeding ? ?
In my opinion the answer In my opinion the answer is nois no..
As a result of quantitative genetic analyses, we do not think that one should make control crosses for avocado breeding. The reason is that the definition of the optimal avocado cultivar isquite wide (one can think of very different ladies that are bothbeautiful and smart…..)
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Conventional BreedingConventional Breeding::
Only a few avocado breeding projects existOnly a few avocado breeding projects exist(most current commercial cultivars are randomly (most current commercial cultivars are randomly selected seedlingsselected seedlings..))
The breeding process composed of: open The breeding process composed of: open pollinations (or controlled crosses); generation pollinations (or controlled crosses); generation of thousands of seedlings (and more); selection; of thousands of seedlings (and more); selection; grafting and testinggrafting and testing
The basics of classical breeding.
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A.A. The breeding orchard (not too dense)The breeding orchard (not too dense)Aiming at selection of the best 1Aiming at selection of the best 1--5% based on5% based onfruit characteristicsfruit characteristics
B.B. Assessment of the "interesting" seedlings Assessment of the "interesting" seedlings (commercial conditions)(commercial conditions) Aiming at testing for yield and storage capacities, Aiming at testing for yield and storage capacities, in duplicates, under various conditions, by in duplicates, under various conditions, by various experts, generation of stock for budvarious experts, generation of stock for bud--woodwood
We Suggest a Two Phase ProgramWe Suggest a Two Phase Program::
We recommend classical breeding to be carried out in two phases as explained.
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Fruit characteristics: The "wine" and the "bottleFruit characteristics: The "wine" and the "bottle""
““QualityQuality”” and and ““AppearanceAppearance””
Tree characteristics: mainly: YieldTree characteristics: mainly: Yield
Goals: The "Perfect LadyGoals: The "Perfect Lady""
Breeding is like searching for the perfect lady (perfect guys do not exist….). Thus, the goal is to combine beautiful appearance with high quality. In addition, some tree traits should also be considered.
.
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YieldYield
Fruit ShapeFruit Shape
Harvest SeasonHarvest Season
Conclusion:Conclusion: Focus on Goals Unobtainable by Focus on Goals Unobtainable by Horticultural MethodologiesHorticultural Methodologies
Present Examples: Present Examples: Nutritional Value; resistanceNutritional Value; resistance
Impact of new HorticulturalImpact of new Horticultural
Developments on BreedingDevelopments on Breeding
Development in agro technologies, have impact on breeding. If one can affect yield, shape and harvest season by agro technologies, one can focus in breeding on resistance to pests and diseases and nutritional values.
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New Avocado Cultivars New Avocado Cultivars Developed in the Last 15 YearsDeveloped in the Last 15 Years
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40/100 – Eden
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Eden
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Iriet
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Gwen